Sessile Legionella pneumophila is able to grow on surfaces and generate structured monospecies biofilms

Currently, models for studying Legionella pneumophila biofilm formation rely on multi-species biofilms with low reproducibility or on growth in rich medium, where planktonic growth is unavoidable. The present study describes a new medium adapted to the growth of L. pneumophila monospecies biofilms in vitro. A microplate model was used to test several media. After incubation for 6 days in a specific biofilm broth not supporting planktonic growth, biofilms consisted of 5.36 ± 0.40 log (cfu cm−2) or 5.34 ± 0.33 log (gu cm−2). The adhered population remained stable for up to 3 weeks after initial inoculation. In situ confocal microscope observations revealed a typical biofilm structure, comprising cell clusters ranging up to 300 μm in height. This model is adapted to growing monospecies L. pneumophila biofilms that are structurally different from biofilms formed in a rich medium. High reproducibility and the absence of other microbial species make this model useful for studying genes involved in biofilm formation

La fouille de textes au service de la documentation

Article de vulgarisation scientifiqueNational audienceLes masses de données textuelles aujourd'hui disponibles engendrent un problème spécifique lié à leur traitement automatique. Des méthodes de fouille de textes et de traitement automatique du langage peuvent en partie répondre à cette difficulté. Approche des procédés et des nouveaux défis à relever présentés par deux chercheurs du Cirad, centre de recherche français qui répond, avec les pays du Sud, aux enjeux internationaux de l'agriculture et du développement

Can Facebook's community standards keep up with legal certainty? Content moderation governance under the pressure of the Digital Services Act

Content moderation by social media companies is a challenge for regulators around the world. The European Union is trying to tackle this challenge with its Digital Services Act (DSA). Notably, Article 14 DSA aims to impose language requirements based on the principle of legal certainty to social media companies' terms and conditions, of which community standards (CS) are a part. The principle of legal certainty is one of the building blocks of international human rights law, and its inclusion in the DSA illustrates the human rights-based approach anchored in this European regulation. Based on a content analysis of Facebook's CS, this paper shows that their standards do not meet European requirements for legal certainty and argues that important changes in content moderation governance would be needed for proper compliance. Such changes could generate a domino effect beyond the European Union and on Facebook's content moderation governance itself. At the same time, the DSA could also generate a boomerang effect on the legal certainty principle as such. From this perspective, the paper contributes to the literature on regulatory governance studies, and on international human rights law in social media studies from a European Union perspective

Benefits and limitations of data assimilation for discharge forecasting using an event-based rainfall–runoff model

Mediterranean catchments in southern France are threatened by potentially devastating fast floods which are difficult to anticipate. In order to improve the skill of rainfall-runoff models in predicting such flash floods, hydrologists use data assimilation techniques to provide real-time updates of the model using observational data. This approach seeks to reduce the uncertainties present in different components of the hydrological model (forcing, parameters or state variables) in order to minimize the error in simulated discharges. This article presents a data assimilation procedure, the best linear unbiased estimator (BLUE), used with the goal of improving the peak discharge predictions generated by an event-based hydrological model Soil Conservation Service lag and route (SCS-LR). For a given prediction date, selected model inputs are corrected by assimilating discharge data observed at the basin outlet. This study is conducted on the Lez Mediterranean basin in southern France. The key objectives of this article are (i) to select the parameter(s) which allow for the most efficient and reliable correction of the simulated discharges, (ii) to demonstrate the impact of the correction of the initial condition upon simulated discharges, and (iii) to identify and understand conditions in which this technique fails to improve the forecast skill. The correction of the initial moisture deficit of the soil reservoir proves to be the most efficient control parameter for adjusting the peak discharge. Using data assimilation, this correction leads to an average of 12% improvement in the flood peak magnitude forecast in 75% of cases. The investigation of the other 25% of cases points out a number of precautions for the appropriate use of this data assimilation procedure

Hydrothermal synthesis of nanosized BaTiO3 powders and dielectric properties of corresponding ceramics

BaTiO3 fine powders were synthesized by hydrothermal method at 150 °C or 250 °C for 7 h, starting from a mixture of TiCl3 + BaCl2 or TiO2 + BaCl2. The size of the crystallites is close to 20 nm whatever the starting mixture and the reaction temperature. These powders are well crystallized and constituted of a mixture of the metastable cubic and stable tetragonal phases. The ceramics obtained after uniaxial pressing and sintering at 1250 °C for 10 h or 20 h present high densification (up to 99.8%). The Curie temperature (Tc) and the electrical permittivity ( r) of the ceramics strongly depend on the type of titanium source that has been used for preparing the powder and on the sintering dwell time. Particularly, Tc is shifted towards lower temperature when TiCl3 is used. The permittivity value at Tc of BaTiO3 sintered at 1250 °C for 10 h reaches 7000 and 11,000 with respectively TiCl3 and TiO2 used as titanium source

Correcting the radar rainfall forcing of a hydrological model with data assimilation: application to flood forecasting in the Lez Catchment in Southern France

The present study explores the application of a data assimilation (DA) procedure to correct the radar rain- fall inputs of an event-based, distributed, parsimonious hy- drological model. An extended Kalman filter algorithm was built on top of a rainfall-runoff model in order to assimilate discharge observations at the catchment outlet. This work fo- cuses primarily on the uncertainty in the rainfall data and considers this as the principal source of error in the sim- ulated discharges, neglecting simplifications in the hydro- logical model structure and poor knowledge of catchment physics. The study site is the 114 km2 Lez catchment near Montpellier, France. This catchment is subject to heavy oro- graphic rainfall and characterised by a karstic geology, lead- ing to flash flooding events. The hydrological model uses a derived version of the SCS method, combined with a Lag and Route transfer function. Because the radar rainfall in- put to the model depends on geographical features and cloud structures, it is particularly uncertain and results in signifi- cant errors in the simulated discharges. This study seeks to demonstrate that a simple DA algorithm is capable of ren- dering radar rainfall suitable for hydrological forecasting. To test this hypothesis, the DA analysis was applied to estimate a constant hyetograph correction to each of 19 flood events. The analysis was carried in two different modes: by assimi- lating observations at all available time steps, referred to here as reanalysis mode, and by using only observations up to 3 h before the flood peak to mimic an operational environment, referred to as pseudo-forecast mode. In reanalysis mode, the resulting correction of the radar rainfall data was then com- pared to the mean field bias (MFB), a corrective coefficient determined using rain gauge measurements. It was shown that the radar rainfall corrected using DA leads to improved discharge simulations and Nash-Sutcliffe efficiency criteria compared to the MFB correction. In pseudo-forecast mode, the reduction of the uncertainty in the rainfall data leads to a reduction of the error in the simulated discharge, but un- certainty from the model parameterisation diminishes data assimilation efficiency. While the DA algorithm used is this study is effective in correcting uncertain radar rainfall, model uncertainty remains an important challenge for flood fore- casting within the Lez catchment

Les mécanismes sous-jacents aux effets pathologiques cardiaques de l’angiotensine II dans le remodelage électrique et contractile entre les sexes

L’insuffisance cardiaque (IC) est associée à un taux de mortalité et d’hospitalisations élevé causant un fardeau économique important. Les deux causes majeures de décès de l’IC sont les arythmies ventriculaires létales et les sidérations myocardiques. Il est maintenant reconnu que l’angiotensine II (ANGII) est l'un des principaux médiateurs de l’IC. Ses effets délétères découlent de l’activation du récepteur de type 1 de l’ANGII (AT1) et entraînent le développement d’hypertrophie. Toutefois, son rôle dans la genèse d’arythmies demeure incompris. De ce fait, l'étude des mécanismes électriques et contractiles sous-jacents aux effets pathologiques de l’ANGII s’avère essentielle afin de mieux comprendre et soigner cette pathologie. Il est souvent perçu que les femmes sont protégées envers les maladies cardiovasculaires. Cependant, le nombre total de femmes décédant d’IC est plus grand que le nombre d’hommes. Également, l’impact des facteurs de risque diffère entre chaque sexe. Ces différences existent, mais les mécanismes sous-jacents sont encore peu connus. De plus, les femmes reçoivent fréquemment un diagnostic ou un traitement inapproprié en raison d’un manque d’information sur les différences entre les sexes dans la manifestation d’une pathologie. Ce manque de données peut découler du fait que les sujets de sexe féminin sont souvent sous-représentés dans les essais cliniques ou la recherche fondamentale ce qui a grandement limité l’avancement de nos connaissances sur ~50 % de la population. Ainsi, il semble plus que nécessaire d’approfondir notre compréhension des différences entre les sexes, notamment dans la progression de l’IC. L’utilisation d’un modèle de souris transgénique surexprimant le récepteur AT1 (souris AT1R) a permis d’étudier les changements électriques, structurels et contractiles avant et après le développement d’hypertrophie. Premièrement, chez les souris AT1R mâles, un ralentissement de la conduction ventriculaire a été observé indépendamment de l’hypertrophie. Ce résultat était expliqué par une réduction de la densité du courant Na+, mais pas de l’expression du canal. Ensuite, le rôle des protéines kinases C (PKC) dans la régulation du canal Na+ par l’ANGII a été exploré. Les évidences ont suggéré que la PKCα était responsable de la modulation de la diminution du courant Na+ chez les souris AT1R mâles et dans les cardiomyocytes humains dérivés de cellules souches induites pluripotentes (hiPSC-CM) en réponse à un traitement chronique à l’ANGII. Ensuite, les différences entre les sexes ont été comparées chez la souris AT1R. Une plus grande mortalité a été constatée chez les femelles AT1R suggérant qu’elles sont plus sensibles à la surexpression de AT1R. Le remodelage électrique ventriculaire a donc été comparé entre les souris AT1R des deux sexes. Les courants ioniques étaient altérés de façon similaire entre les sexes excluant ainsi leur implication dans la mortalité plus élevée chez les femelles. Ensuite, l’homéostasie calcique et la fonction cardiaque ont été étudiées. Il a été démontré que les femelles développaient une hypertrophie et une dilatation ventriculaire plus sévère que les mâles. De plus, les femelles AT1R avaient de petits transitoires calciques, une extrusion du Ca2+ plus lente ainsi qu’une augmentation de la fréquence des étincelles Ca2+ pouvant participer à des troubles contractiles et à la venue de post-dépolarisations précoces. En conclusion, l’ANGII est impliquée dans le remodelage électrique, structurel et calcique associé à l'émergence de l’IC. De surcroît, ces altérations affectent plus sévèrement les femelles soulignant la présence de différences entre les sexes dans le développement de l’IC.Heart failure (HF) is associated with a high mortality and hospitalisations rate causing an important economic burden. The two main causes of mortality in HF are severe ventricular arrhythmias and myocardial stunning. It is now recognized that angiotensin II (ANGII) is one of the main effectors of HF and its deleterious roles are mediated through the activation of the ANGII type 1 (AT1) receptor. Even though it has been shown that ANGII can induce cardiac hypertrophy, its impact on cardiac arrhythmias remains incompletely understood. Therefore, it seems essential to better understand the underlying electrical and contractile mechanisms associated with a chronic ANGII stimulation in order to improve HF therapy. There is a misperception that women are protected against cardiovascular diseases. Nevertheless, a higher number of women die of HF than men. Risk factors also impact differently men and women. The mechanisms underlying these differences are still poorly understood. Furthermore, women frequently receive an inappropriate diagnosis or treatment because of unacknowledged sex differences. This lack of knowledge may result from the fact that women and female animals are too often underrepresented in clinical trials or basic research studies even though they represent 50 % of the population. As a consequence, our knowledge on women pathology is significantly limited. Hence, these observations warrant further investigation to better comprehend sex differences especially in the manifestation and progression of HF. Transgenic mice with a cardiomyocyte specific overexpression of the AT1 receptor (AT1R) have been used to study the electrical, structural and contractile changes occurring before and after the development of hypertrophy. It was first observed in AT1R male mice that ventricular conduction was slowed independently of hypertrophy. This result was due to a reduction in Na+ current density, but not channel expression. Consequently, the involvement of protein kinase C (PKC) in the regulation of the Na+ current by ANGII was studied and results obtained from mouse and human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CM) suggest that PKCα is responsible for the reduction in Na+ current observed in AT1R male mice and after chronic ANGII exposure. Subsequently, sex differences were also investigated in AT1R mice. We observed that female AT1R mice have a higher mortality rate compared to males suggesting they are more sensitive to AT1R overexpression. Since ventricular electrical remodeling was similarly altered between AT1R mice of both sexes it could not explain the higher mortality rate of AT1R females. Accordingly, cardiac function and Ca2+ dynamics were evaluated next and the data showed that AT1R females develop more severe ventricular hypertrophy and dilation than males. In addition, AT1R females had a small Ca2+ transients, slower Ca2+ reuptake along with a higher Ca2+ spark frequency which could contribute to poor contraction and cardiac arrhythmias through the promotion of delayed after depolarization. In conclusion, ANGII is implicated in electrical, structural and Ca2+ handling remodeling that promotes poor prognosis associated with HF. Furthermore, these alterations affected female mice more severely highlighting important sex differences in the manifestation of HF