In Vivo Dissection of the Intrinsically Disordered Receptor Domain of Tim23

In the intermembrane space (IMS) of mitochondria, the receptor domain of Tim23 has an essential role during translocation of hundreds of different proteins from the cytosol via the TOM and TIM23 complexes in the outer and inner membranes, respectively. This intrinsically disordered domain, which can even extend into the cytosol, was shown, mostly in vitro, to interact with several subunits of the TOM and TIM23 complexes. To obtain molecular understanding of this organizational hub in the IMS, we dissected the IMS domain of Tim23 in vivo. We show that the interaction surface of Tim23 with Tim50 is larger than previously thought and reveal an unexpected interaction of Tim23 with Pam17 in the IMS, impairment of which influences their interaction in the matrix. Furthermore, mutations of two conserved negatively charged residues of Tim23, close to the inner membrane, prevented dimerization of Tim23. The same mutations increased exposure of Tim23 on the mitochondrial surface, whereas dissipation of membrane potential decreased it. Our results reveal an intricate network of Tim23 interactions in the IMS, whose influence is transduced across two mitochondrial membranes, ensuring efficient translocation of proteins into mitochondria. (C) 2020 The Author(s). Published by Elsevier Ltd

Search for hidden-photon dark matter with the FUNK experiment

Many extensions of the Standard Model of particle physics predict a parallel sector of a new U(1) symmetry, giving rise to hidden photons. These hidden photons are candidate particles for cold dark matter. They are expected to kinetically mix with regular photons, which leads to a tiny oscillating electric-field component accompanying dark matter particles. A conducting surface can convert such dark matter particles into photons which are emitted almost perpendicularly to the surface. The corresponding photon frequency follows from the mass of the hidden photons. In this contribution we present a preliminary result on a hidden photon search in the visible and near-UV wavelength range that was done with a large, 14 m2 spherical metallic mirror and discuss future dark matter searches in the eV and sub-eV range by application of different detectors for electromagnetic radiation.Comment: Contribution to the 35th International Cosmic Ray Conference ICRC2017, 10 to 20 July, 2017, Bexco, Busan, Korea. arXiv admin note: text overlap with arXiv:1711.0296

BLUF Domain Function Does Not Require a Metastable Radical Intermediate State

BLUF (blue light using flavin) domain proteins are an important family of blue light-sensing proteins which control a wide variety of functions in cells. The primary light-activated step in the BLUF domain is not yet established. A number of experimental and theoretical studies points to a role for photoinduced electron transfer (PET) between a highly conserved tyrosine and the flavin chromophore to form a radical intermediate state. Here we investigate the role of PET in three different BLUF proteins, using ultrafast broadband transient infrared spectroscopy. We characterize and identify infrared active marker modes for excited and ground state species and use them to record photochemical dynamics in the proteins. We also generate mutants which unambiguously show PET and, through isotope labeling of the protein and the chromophore, are able to assign modes characteristic of both flavin and protein radical states. We find that these radical intermediates are not observed in two of the three BLUF domains studied, casting doubt on the importance of the formation of a population of radical intermediates in the BLUF photocycle. Further, unnatural amino acid mutagenesis is used to replace the conserved tyrosine with fluorotyrosines, thus modifying the driving force for the proposed electron transfer reaction; the rate changes observed are also not consistent with a PET mechanism. Thus, while intermediates of PET reactions can be observed in BLUF proteins they are not correlated with photoactivity, suggesting that radical intermediates are not central to their operation. Alternative nonradical pathways including a keto–enol tautomerization induced by electronic excitation of the flavin ring are considered

Search for dark photons as candidates for Dark Matter with FUNK

An additional U(1) symmetry predicted in theories beyond the Standard Model of particle physics can give rise to hidden (dark) photons. Depending on the mass and density of these hidden photons, they could account for a large fraction of the Dark Matter observed in the Universe. When passing through an interface of materials with different dielectric properties, hidden photons are expected to produce a tiny flux of photons. The wavelength of these photons is directly related to the mass of the hidden photons. In this contribution we report on measurements covering the visible and near-UV spectrum, corresponding to a dark photon mass in the eV range. The data were taken with the FUNK experiment using a spherical mirror of ~14m2 total area built up of 36 aluminum segments

Large scale cosmic-ray anisotropy with KASCADE

The results of an analysis of the large scale anisotropy of cosmic rays in the PeV range are presented. The Rayleigh formalism is applied to the right ascension distribution of extensive air showers measured by the KASCADE experiment.The data set contains about 10^8 extensive air showers in the energy range from 0.7 to 6 PeV. No hints for anisotropy are visible in the right ascension distributions in this energy range. This accounts for all showers as well as for subsets containing showers induced by predominantly light respectively heavy primary particles. Upper flux limits for Rayleigh amplitudes are determined to be between 10^-3 at 0.7 PeV and 10^-2 at 6 PeV primary energy.Comment: accepted by The Astrophysical Journa

Primary Proton Spectrum of Cosmic Rays measured with Single Hadrons

The flux of cosmic-ray induced single hadrons near sea level has been measured with the large hadron calorimeter of the KASCADE experiment. The measurement corroborates former results obtained with detectors of smaller size if the enlarged veto of the 304 m^2 calorimeter surface is encounted for. The program CORSIKA/QGSJET is used to compute the cosmic-ray flux above the atmosphere. Between E_0=300 GeV and 1 PeV the primary proton spectrum can be described with a power law parametrized as dJ/dE_0=(0.15+-0.03)*E_0^{-2.78+-0.03} m^-2 s^-1 sr^-1 TeV^-1. In the TeV region the proton flux compares well with the results from recent measurements of direct experiments.Comment: 13 pages, accepted by Astrophysical Journa

A comparison of the cosmic-ray energy scales of Tunka-133 and KASCADE-Grande via their radio extensions Tunka-Rex and LOPES

The radio technique is a promising method for detection of cosmic-ray air showers of energies around $100\,$PeV and higher with an array of radio antennas. Since the amplitude of the radio signal can be measured absolutely and increases with the shower energy, radio measurements can be used to determine the air-shower energy on an absolute scale. We show that calibrated measurements of radio detectors operated in coincidence with host experiments measuring air showers based on other techniques can be used for comparing the energy scales of these host experiments. Using two approaches, first via direct amplitude measurements, and second via comparison of measurements with air shower simulations, we compare the energy scales of the air-shower experiments Tunka-133 and KASCADE-Grande, using their radio extensions, Tunka-Rex and LOPES, respectively. Due to the consistent amplitude calibration for Tunka-Rex and LOPES achieved by using the same reference source, this comparison reaches an accuracy of approximately $10\,\%$ - limited by some shortcomings of LOPES, which was a prototype experiment for the digital radio technique for air showers. In particular we show that the energy scales of cosmic-ray measurements by the independently calibrated experiments KASCADE-Grande and Tunka-133 are consistent with each other on this level

Dissecting the knee - Air shower measurements with KASCADE

Recent results of the KASCADE air shower experiment are presented in order to shed some light on the astrophysics of cosmic rays in the region of the knee in the energy spectrum. The results include investigations of high-energy interactions in the atmosphere, the analysis of the arrival directions of cosmic rays, the determination of the mean logarithmic mass, and the unfolding of energy spectra for elemental groups

KASCADE: Astrophysical results and tests of hadronic interaction models

KASCADE is a multi-detector setup to get redundant information on single air shower basis. The information is used to perform multiparameter analyses to solve the threefold problem of the reconstruction of (i)the unknown primary energy, (ii) the primary mass, and (iii) to quantify the characteristics of the hadronic interactions in the air-shower development. In this talk recent results of the KASCADE data analyses are summarized concerning cosmic ray anisotropy studies, determination of flux spectra for different primary mass groups, and approaches to test hadronic interaction models. Neither large scale anisotropies nor point sources were found in the KASCADE data set. The energy spectra of the light element groups result in a knee-like bending and a steepening above the knee. The topology of the individual knee positions shows a dependency on the primary particle. Though no hadronic interaction model is fully able to describe the multi-parameter data of KASCADE consistently, the more recent models or improved versions of older models reproduce the data better than few years ago.Comment: to appear in Nucl. Phys. B (Proc. Suppl.), Proc. of the XIII ISVHECRI, Pylos 2004 - with a better quality of the figure