Knowledge and attitude of physicians about cupping in Chaharmahal va Bakhtiari province, 2007

چکیده: زمینه و هدف: از جمله روشهای اصلی در طب تکمیلی حجامت است. با توجه به افزایش رویکرد جدید به انجام حجامت، این تحقیق به بررسی آگاهی و نگرش پزشکان استان چهارمحال و بختیاری نسبت به حجامت پرداخته است. روش بررسی: در این مطالعه توصیفی تحلیلی 152 پزشک به روش تصادفی سیستماتیک انتخاب شدند. اطلاعات با استفاده از پرسشنامه خود ساخته که شامل مشخصات دموگرافیک، سوالات آگاهی و نگرش بود جمع آوری و داده ها با استفاده از آزمون های آماری ضریب همبستگی اسپیرمن، t، آزمون تعقیبی دانکن و آنالیز واریانس مورد تجزیه و تحلیل قرار گرفت. یافته ها: از مجموع 152 نفر پزشکان مورد بررسی 78 نفر (3/51) زن و بقیه مرد بودند. میانگین سن پزشکان 6±9/33 سال بود. 16 افراد آگاهی خوب و 18 نمونه ها نگرش بالا (مثبت) نسبت به حجامت داشتند. بین سن، جنس و محل خدمت پزشکان با میزان آگاهی آنان ارتباط آماری معنی داری وجود نداشت. همچنین میزان آگاهی پزشکان عمومی و متخصص در خصوص حجامت با هم فرقی نداشت. بین نگرش نسبت به حجامت با وضعیت استخدامی پزشکان ارتباط معنی داری وجود داشت به گونه ای که پزشکان طرحی نگرش پایین تری نسبت به سایر پزشکان داشتند (001/0

DFNB59 Gene Mutations and its Association with Deafness in Schoolchildren in Kohgilooyeh & Boyerahmad Province

Introduction & Objective: Hearing loss is a common disease affecting millions of people worldwide. Hearing loss can be caused due to genetic or environmental factors or even both. The genetic of hearing defect is highly heterogeneous and more than 100 genes are predicted to cause this disorder in humans. A newly identified gene (DFNB59) has been shown to cause deafness in some populations. Here we report mutation analysis for DFNB59 gene in 88 genetic non-syndromic hearing loss subjects. Materials & Methods: In this descriptive-lab based study which was conducted at the Cellular and Molecular Research Center of Shahrekord University of Medical Sciences, DNA was extracted from the peripheral blood samples using standard phenol chloroform procedure. Mutation analysis for DFNB59 gene was performed using PCR-SSCP/HA protocol. The suspected DFNB59 which was detected as shifted bands on PAGE were then confirmed by direct sequencing strategy. Results: Two DFNB59 polymorphisms including c.793C>G and c.793C>T were detected in 8 and 1 deaf subjects respectively. Conclusion: We conclude that there is no association between DFNB59 mutations and deafness in the studied patients in the region

DFNB59 Gene Mutation Screening Using PCR-SSCP/HA Technique in Non-syndromic Genetic Hearing Loss in Bushehr Province

Background: Hearing impairment (HI) is the most prevalent Neurosensory disorder which is heterogenous and can also occur due to environmental causes. The majority of hearing deficiencies are of genetic origin affecting about 60% of the HI cases. A novel gene DFNB59 encodes pejvakin has been recently shown to cause deafness. This study aims to determine the frequency of DFNB59 gene mutations in coding region the gene in Bushehr province. Methods: In this descriptive experimental study, we investigated the presence of DFNB59

DFNB59 gene mutations screening in non syndromic deaf subjects in Chaharmahal va Bakhtiari province

زمینه و هدف: وقوع ناشنوایی پیش زبانی حدود 1 در 1000 تولد است که بیش از 60 موارد آن ارثی هستند. ناشنوایی اختلالی هتروژن محسوب می شود و ممکن است به علل محیطی، ژنتیکی یا هر دو رخ دهد. اخیراً جهش های ژن DFNB59 که رمز کننده پروتئین پژواکین است به عنوان عامل ناشنوایی نوع عصبی معرفی شده اند. این مطالعه با هدف بررسی نوع و فراوانی جهش های ژن DFNB59 در 100 ناشنوای غیرسندرومی، در استان چهارمحال و بختیاری انجام شد. روش بررسی: در این مطالعه توصیفی- آزمایشگاهی، فراوانی جهش های ژنDFNB59 در کل اگزون های کد کننده این ژن بررسی گردید. DNA از نمونه های خون محیطی به روش استاندارد فنل کلروفرم استخراج شد. وجود جهش های DFNB59 با روش غربالگری /Heteroduplex Analysis (HA) PCR- SSCP بررسی گردید. سپس جهش های مشاهده شده در اگزون های 2 و 4 با استفاده از روش PCR- RFlP اگزون شماره 6 با استفاده ار تکنیک Nested PCR و اگزون شماره 7 به کمک تعیین توالی تایید گردید. یافته ها: در این تحقیق 3 نوع پلی مورفیسم ژنی (793C>T 793C>G و 874G>A) و یک نوع جهش 988delG به ترتیب با فراوانی 7، 5، 2 و 1 شناسایی شد. نتیجه گیری: مطالعه حاضر و تحقیقات قبلی حاکی از نقش اندک جهش های ژن پژواکین در ایجاد ناشنوایی در استان چهارمحال و بختیاری است و نتیجه اینکه از نظر بالینی جهش های ژن DFNB59 اهمیت چندانی در این منطقه ندارند

Evaluation of the Effectiveness of the Carbon Dioxide (CO2) Laser in Minor Oral Surgery: A Systematic Review: CO2 laser and minor oral surgery

Introduction: Lasers in oral surgery have been extensively studied in recent years. Laser treatment is now a well-known technology that is frequently employed on oral soft tissues. The carbon dioxide (CO2) laser was one of the first soft tissue removal lasers. Because of the strong affinity of the CO2 laser to water, it is best used for removing, vaporizing, and coagulating these tissues. In minor oral surgery, CO2 laser therapy has shown advantages. Therefore, this study examined the CO2 laser used in minor oral soft tissue surgery.Methods: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards were followed in this study. A question for research encompassing the inclusion criteria for the participants, intervention, comparison, outcome, and study design (PICOS) was formulated. The search queries were entered into the PubMed/Medline, Scopus, and Embase databases. Consideration was given to publications published between January 1, 2018, and March 15, 2023.Results: The research included 37 studies after narrowing search results, eliminating duplicate titles,    and conducting an eligibility review (three animal studies, seven case reports, three case series, and twenty-four clinical studies). CO2 lasers alone or in combination with other therapies successfully treated oral potentially malignant disorders (OPMDs), oral tumors, oral fibrous-epithelial lesions, gingival melanin hyperpigmentation, pyogenic granuloma, socket preservation, mucocele, high labial frenulum attachment, and so on. CO2 lasers reduced intra- and postoperative complications and adverse effects, improved postoperative functional results, ablated tissues with precision, and minimized disease recurrence and malignant transformation.Conclusion: Our study found that the CO2 laser in oral minor surgeries is successful, but further randomized clinical trials and multicenter studies are recommended to compare CO2 laser surgery to other treatments

Celecoxib or Prednisolone for Treatment of Medication Overuse Headache: A Randomized, Double-Blind Clinical Trial in Migrainous Patients

Background:Treatment of Medication Overuse Headache (MOH) is yet under debate and Celecoxib as a Cyclooxygenase 2 (COX2) -inhibitor has not been tried widely as a pain relief drug for this type of headaches in migrainous patients.  Objectives: comparing the efficacy of celecoxib versus prednisolone for withdrawal period of MOH. Materials & Methods: A double-blind, randomized clinical trial was carried out, on 75 patients with MOH who visited the Isfahan Neurology Clinic in 2016. They were assigned into two groups of oral prednisolone and celecoxib prescribed for 15 days. Any changes in the duration (average hours of daily headache), frequency, and severity of headaches, and intake of rescue medication and their side effects were recorded after the 15-day intervention period. Data were analyzed by independent t, paired t, Mann-Whitney, Wilcoxon test, and chi-square tests in SPSS software version 20. Results: Average duration of headache in both groups significantly decreased after treatment (P<0.001). It decreased significantly in celecoxib group (P=0.04). Headache frequency decreased more but not significantly in the celecoxib group (P=0.08). Considering MIGSEV (Migraine Severity) and VAS (Visual Analogue Scale) scales, headache severity alleviated significantly after treatment in both groups (P<0.001). The decrease was more noticeable in the celecoxib group. Need for rescue medications (P=0.048), and side effect appearance (P=0.001) was also lower in patients who took celecoxib, compared to the other group. Conclusion: Celecoxib showed higher efficacy and fewer side effects, compared to prednisolone in treatment of medication overuse headache in migrainous patients