TREATMENT OF HEMOPHILIA WITH HUMAN FACTORIX PRODUCED IN MAMIMARY TISSUE OF TRANSGENIC MAMMALS

Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5\u27-untranslated and 3\u27-un-translated region, which is substituted with a 5- and 3\u27-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described

Localizing Project

Like many extension information offices, the Virginia Tech office prepares subject-matter news releases and sends them weekly to all 130 newspapers in the state

TREATMENT OF HEMOPHILIA WITH HUMAN FACTORIX PRODUCED IN MAMIMARY TISSUE OF TRANSGENIC MAMMALS

Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5\u27-untranslated and 3\u27-un-translated region, which is substituted with a 5- and 3\u27-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described

Nutrition and pregnancy

"MP 461""Prepared by Dr. Mary Jean Baker in collaboration with Dr. Evelyn H. Johnson, Dr. Rhonwyn Lowry, and Jean Brand, with consultation of a committee of county and State Extension workers. Extension Service, U.S. Department of Agriculture.

Water

"MP 459""Prepared by Dr. Mary Jean Baker in collaboration with Dr. Evelyn H. Johnson, Dr. Rhonwyn Lowry, and Jean Brand, with consultation of a committee of county and State Extension workers. Extension Service, U. S. Department of Agriculture.

Nutrition and drugs

"MP 460""Prepared by Dr. Mary Jean Baker in collaboration with Dr. Evelyn H. Johnson, Dr. Rohnwyn Lowry, and Jean Brand, with consultation of a committee of county and State Extension workers. Extension Service, U.S. Department of Agriculture.

Calcium

"MP 457""Prepared by Dr. Mary Jean Baker in collaboration with Dr. Evelyn H. Johnson, Dr. Rhonwyn Lowry, and Jean Brand, with consultation of a committee of county and State Extension workers. Extension Service, U. S. Department of Agriculture.

Vitamin A : how does vitamin A affect your driving?

"MP 453""Prepared by Dr. Mary Jean Baker in collaboration with Dr. Evelyn H. Johnson, Dr. Rhonwyn Lowry, and Jean Brand, with consultation of a committee of county and State Extension workers. Extension Service, U.S. Department of Agriculture.

"Bag of tricks" for EFNEP youth program

"November, 1972