Application of nanotechnology in the agro-food sector

Nanotechnology is an emerging field of research that has been widely applied in different scientific and engineering areas. The agro-food sector is not an exception, which considers its applicability in several areas of major interest for both consumers and producers. This review considers major concepts related to nanostructures and nano-based instruments used in the food sector, as well as their applications in agro-food products. Food safety through the use of nanosensors for pathogen detection, smart packaging, and valorisation of food products by nanoencapsulation/nanodelivery of food ingredients (e.g. flavours) are examples of important areas of nanotechnology. Consumers' apprehension regarding food stability and safety issues is also considered.info:eu-repo/semantics/publishedVersio

Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability

© The Animal Consortium 2011In the meat industry, the manipulation of fat deposition in cattle is of pivotal importance to improve production efficiency, carcass composition and ultimately meat quality. There is an increasing interest in the identification of key factors and molecular mechanisms responsible for the development of specific fat depots. This study aimed at elucidating the influence of breed and diet on adipose tissue membrane permeability and fluidity and their interplay on fat deposition in bovines. Two Portuguese autochthonous breeds, Alentejana and Barrosã, recognized as late- and early-maturing breeds, respectively, were chosen to examine the effects of breed and diet on fat deposition and on adipose membrane composition and permeability. Twenty-four male bovines from these breeds were fed on silage-based or concentrate-based diets for 11 months. Animals were slaughtered to determine their live slaughter and hot carcass weights, as well as weights of subcutaneous and visceral adipose depots. Mesenteric fat depots were excised and used to isolate adipocyte membrane vesicles where cholesterol content, fatty acid profile as well as permeability and fluidity were determined. Total accumulation of neither subcutaneous nor visceral fat was influenced by breed. In contrast, mesenteric and omental fat depots weights were higher in concentrate-fed bulls relative to silage-fed animals. Membrane fluidity and permeability to water and glycerol in mesenteric adipose tissue were found to be independent of breed and diet. Moreover, the deposition of cholesterol and unsaturated fatty acids, which may influence membrane properties, were unchanged among experimental groups. Adipose membrane lipids from the mesenteric fat depot of ruminants were rich in saturated fatty acids, and unaffected by polyunsaturated fatty acids dietary levels. Our results provide evidence against the involvement of cellular membrane permeability to glycerol on fat accumulation in mesenteric fat tissue of concentrate-fed bovines, which is consistent with the unchanged membrane lipid profile found among experimental groups.This study was supported by Fundação para a Ciência e a Tecnologia (FCT) through grant PTDC/CVT/2006/66114 and individual fellowships to Ana P. Martins (SFRH/BD/2009/65046), Ana S. H. Costa (SFRH/BD/2009/61068) and Susana V. Martins (SFRH/BPD/2009/63019). Paula A. Lopes is a researcher from the program ‘‘Ciência 2008’’ from FC

Costs and externalities of road transport in Portugal

Urban dispersion (sprawl) is a reality, however unplanned it may be. Its supporters advocate contact with nature, space and intimacy, however disadvantages include land consumption, public infrastructure and mobility costs and housing prices. The Research Project “Costs and Benefits of Urban Dispersion on a local scale” seeks to contribute to the debate with an objective approach based on the quantification of costs, externalities and benefits of different urban settlement patterns. This paper presents one of the Project’s tasks, the one concerning mobility costs, including externalities. Quantified costs include investment, inspection, insurance, energy and maintenance, as well as external social and environmental costs for road transport, the most significant transport mode operating on a local scale. Different methods are combined depending on available data sources in order to achieve figures for each of the cost components per vehicle-km, ton-km and passenger-km at prices of 2005. Preliminary results for direct costs suggest that in light vehicles investment costs are responsible for the largest share of the totals, while energy costs are the most relevant cost component in heavy vehicles. Heavy duty passenger transport is significantly more expensive than their counterparts. Externalities may mount up to around half of the total costs for some road vehicles.publishe

Total Tumor Load Assessed by One-Step Nucleic Acid Amplification Assay as an Intraoperative Predictor for Non-Sentinel Lymph Node Metastasis in Breast Cancer

BACKGROUND: This study aimed to determine the relationship between CK19 mRNA copy number in sentinel lymph nodes (SLN) assessed by one-step nucleic acid amplification (OSNA) technique, and non-sentinel lymph nodes (NSLN) metastization in invasive breast cancer. A model using total tumor load (TTL) obtained by OSNA technique was also constructed to evaluate its predictability. METHODS: We conducted an observational retrospective study including 598 patients with clinically T1-T3 and node negative invasive breast cancer. Of the 88 patients with positive SLN, 58 patients fulfill the inclusion criteria. RESULTS: In the analyzed group 25.86% had at least one positive NSLN in axillary lymph node dissection. Univariate analysis showed that tumor size, TTL and number of SLN macrometastases were predictive factors for NSLN metastases. In multivariate analysis just the TTL was predictive for positive NSLN (OR 2.67; 95% CI 1.06-6.70; P = 0.036). The ROC curve for the model using TTL alone was obtained and an AUC of 0.805 (95% CI 0.69-0.92) was achieved. For TTL >1.9 × 105 copies/μL we got 73.3% sensitivity, 74.4% specificity and 88.9% negative predictive value to predict NSLN metastases. CONCLUSION: When using OSNA technique to evaluate SLN, NSLN metastases can be predicted intraoperatively. This prediction tool could help in decision for axillary lymph node dissection.info:eu-repo/semantics/publishedVersio

TOFtracker: combination of time-of-flight and high-accuracy bidimensional tracking in a single gaseous detector

A 5-gap timing RPC equipped with patterned electrodes coupled to both charge-sensitive and timing circuits yields a time accuracy of 77 ps along with a position accuracy of 38 μm. These results were obtained by calculating the straight-line fit residuals to the positions provided by a 3-layer telescope made out of identical detectors, detecting almost perpendicular cosmic-ray muons. The device may be useful for particle identification by time-of-flight, where simultaneous measurements of trajectory and time are necessary

O sonho – a comprehensive intervention building on poverty fighting

“O Sonho” (The dream) is a Portuguese NGO that works in Setúbal – a city 45 Km from Lisbon. Since 2005 “O Sonho” has been expanding its participation in community settings. In October 2007 “O Sonho” made a protocol with the Portuguese Social Security System, to work for a national poverty ending program – Rendimento Social de Inserção (Social Integration Income), aiming to promote labor, social and community integration of low income population. This program intends to build with families new tracks for their development, on employment, education, housing, health and civil rights and duties. Intending to build a comprehensive intervention, we have been growing in the areas of microcrédit – building a coalition to give credit to promote self-employment; training – doing and promoting training on health promotion and prevention; food supplies and clothes – building coalitions in order to address serious hunger and poverty situations; and housing – promoting and developing mutualist solutions with families for access to houses.We stand for the crucial proactive role of individuals, families and communities in their own development, so we assume as core principles for intervention the trust relationship building; and the family’s capacity and autonomy in their integration process – promoting families’ participation and empowerment –, we base our efforts on finding ways to support people on creating new opportunities for using their strengths to grow out of poverty.We assume an active role on poverty ending and community development, focusing on social entrepreneurship and serving people in its most important development issues.We believe that this way of creating new supporting structures for and with the community helps to promote community development and systems change

Percutaneous Closure of Patent Foramen Ovale after Anterior Spinal Cord Infarction

In patients with a patent foramen ovale (PFO) who have had a cryptogenic ischemic stroke, percutaneous closure reduces its recurrence risk. However, its role in spinal cord infarction (SCI) is less well-established. A few case reports describe the putative causative role of PFO in SCI. We present a case of a teenager with cryptogenic anterior SCI in the setting of a deep vein thrombosis and a high risk-PFO who underwent successful percutaneous closure.info:eu-repo/semantics/publishedVersio

O sonho – a comprehensive intervention building on poverty fighting

“O Sonho” (The dream) is a Portuguese NGO that works in Setúbal – a city 45 Km from Lisbon. Since 2005 “O Sonho” has been expanding its participation in community settings. In October 2007 “O Sonho” made a protocol with the Portuguese Social Security System, to work for a national poverty ending program – Rendimento Social de Inserção (Social Integration Income), aiming to promote labor, social and community integration of low income population. This program intends to build with families new tracks for their development, on employment, education, housing, health and civil rights and duties. Intending to build a comprehensive intervention, we have been growing in the areas of microcrédit – building a coalition to give credit to promote self-employment; training – doing and promoting training on health promotion and prevention; food supplies and clothes – building coalitions in order to address serious hunger and poverty situations; and housing – promoting and developing mutualist solutions with families for access to houses.We stand for the crucial proactive role of individuals, families and communities in their own development, so we assume as core principles for intervention the trust relationship building; and the family’s capacity and autonomy in their integration process – promoting families’ participation and empowerment –, we base our efforts on finding ways to support people on creating new opportunities for using their strengths to grow out of poverty.We assume an active role on poverty ending and community development, focusing on social entrepreneurship and serving people in its most important development issues.We believe that this way of creating new supporting structures for and with the community helps to promote community development and systems change