EVALUATION OF ANTIOXIDANT ACTIVITY AND CHROMATOGRAPHIC PROFILE OF EXTRACTS FROM THE FALSE JABORANDI (PIPER ADUNCUM)

http://dx.doi.org/10.5902/223611704201Evaluation of the antioxidant activity and chromatographic profile of the Piper aduncum (falsejaborandi) extracts. This paper describes the investigation of the chemical composition using theleaves of Piper aduncum (Piperaceae) through methods of extraction envolvind decoction,maceration and ultrasonic bath. The investigation was made by the gas chromatography-massspectrometry/SPME. From these extracts, the antioxidant activity in the presence of a free radicalDPPH was evaluated.http://dx.doi.org/10.5902/223611704201 Evaluation of the antioxidant activity and chromatographic profile of the Piper aduncum (false jaborandi) extracts. This paper describes the investigation of the chemical composition using the leaves of Piper aduncum (Piperaceae) through methods of extraction envolvind decoction, maceration and ultrasonic bath. The investigation was made by the gas chromatography-mass spectrometry/SPME. From these extracts, the antioxidant activity in the presence of a free radical DPPH was evaluated.

Severe form of lymphocutaneous sporotrichosis: a case report

Sporotrichosis is the most frequent subcutaneous mycosis in Latin America. It is caused by species of the genus Sporothrix. Infection in humans occurs through the entry of the fungus into the skin. Zoonotic outbreaks involving cats in the transmission of the disease have been frequently reported. The lymphocutaneous form is the most commonly observed and the upper limbs are the most affected sites. We report a case of a 64-year-old healthy female patient with a lymphocutaneous form with rapid progression of lesions, which was refractory to initial treatment with itraconazole. Treatment with liposomal amphotericin B was performed with a satisfactory resolution, but aesthetic and functional sequelae in the left upper limb were installed

Tratamento em massa para controle das helmintíases intestinais em área endêmica na Amazônia Brasileira

The objective of the present study was to estimate the prevalence of soil-transmitted helminthiasis and evaluate the sanitary conditions and the role of a mass treatment campaign for control of these infections in Santa Isabel do Rio Negro. A cross-sectional survey was carried out in 2002, to obtain data related to the sanitary conditions of the population and fecal samples for parasitological examination in 308 individuals, followed by a mass treatment with albendazole or mebendazole with coverage of 83% of the city population in 2003. A new survey was carried out in 2004, involving 214 individuals, for comparison of the prevalences of intestinal parasitosis before and after the mass treatment. The prevalences of ascariasis, trichuriasis and hookworm infection were 48%; 27% and 21% respectively in 2002. There was a significant decrease for the frequency of infections by Ascaris lumbricoides (p < 0.05; OR / 95% CI = 0.44 / 0.30 - 0.65), Trichuris trichiura (p < 0.05; OR / 95% CI = 0.37 / 0.22 - 0.62), hookworm (p < 0.05; OR / 95% CI = 0.03 / 0.01 - 0.15) and helminth poliparasitism (p < 0.05; OR / 95% CI = 0.16 / 0.08 - 0.32). It was also noticed a decrease of prevalence of infection by Entamoeba histolytica / dispar (p < 0.05; OR / 95% CI = 0.30 / 0.19 - 0.49) and non-pathogenic amoebas. It was inferred that a mass treatment can contribute to the control of soil-transmitted helminthiasis as a practicable short-dated measure. However, governmental plans for public health, education and urban infrastructure are essential for the sustained reduction of prevalences of those infections.O presente trabalho objetivou avaliar a prevalência e o papel de um tratamento em massa das helmintíases intestinais em Santa Isabel do Rio Negro, Estado do Amazonas, Brasil. Foi realizado em 2002 um estudo seccional, incluindo inquérito copro-parasitológico, objetivando a obtenção das prevalências das parasitoses intestinais e dados sobre as condições sanitárias do local, estudando-se uma amostra de 308 indivíduos. Em 2003 foi realizada intervenção para tratamento em massa das helmintíases intestinais com administração de albendazol (ou mebendazol para crianças entre 12 e 24 meses) na sede do município, alcançando-se 83% de cobertura. Novo inquérito copro-parasitológico foi realizado em 2004, para comparação das prevalências antes a após o tratamento. As prevalências das infecções por Ascaris lumbricoides, Trichuris trichiura e ancilostomídeos foram 48%, 27% e 21%, respectivamente em 2002. Em 2004 observou-se redução significativa das infecções por Ascaris lumbricoides (p < 0,05; OR / 95% IC = 0,44 / 0,30 - 0,65), Trichuris trichiura (p < 0,05; OR / 95% IC = 0,37 / 0,22 - 0,62), ancilostomídeos (p < 0,05; OR / 95% IC = 0,03 / 0,01 - 0,15) e poliparasitismo por helmintos intestinais (p < 0,05; OR / 95% IC = 0,16 / 0,08 - 0,32). Foi também observada redução da prevalência de infecção por Entamoeba histolytica/dispar (p < 0,05; OR / 95% CI = 0,30 / 0,19 - 0,49). Concluiu-se que o tratamento em massa pode auxiliar o controle das helmintíases intestinais, porém ações governamentais em infraestrutura urbana e educação são essenciais para uma redução sustentada das prevalências destas infecções

Perfil nutricional e consumo de suplemento alimentar de atletas de futebol em um clube profissional de São Luís-MA

A nutrição adequada não pode compensar a falta de habilidade ou condicionamento físico deficiente, mas pode ajudar o jogador a potencializar sua habilidade e rendimento. Um atleta bem nutrido rende mais, se recupera mais rápido e consegue melhores resultados. O presente estudo tem como objetivo avaliar o perfil nutricional e o consumo de suplementos de atletas em um clube de futebol de São Luís-MA. A população do estudo foi constituída por 26 atletas do naipe masculino com idade entre 18 a 35 anos, entretanto apenas 21 atletas foram recrutados de acordo com os critérios de seleção. Para coleta de dados fora aplicado o recordatório de 24h, para a mensuração da massa corporal total e a estatura foi utilizada a balança com estadiomêtro (Welmy – modelo W300).  As variáveis foram avaliadas através do software BioEstat 5.0. Para definição do o perfil nutricional foi utilizado o Software Avanutri 9.0. Os atletas apresentaram idade de 25,67 ±3,83 anos, estatura de 1,76± 0,08 metros; massa corporal de 74,86 ± 6,53 Kg; IMC de 24,07 ± 1,08 Kg/ m, os atletas apresentaram um déficit na proporção entre gasto energético total e o valor energético Gasto Energético total de 3.574,35 ± 505,99 Kcal; Valor Energético Total de 2.926,86 ± 420,59 Kcal. O consumo de suplementos alimentares é significante chegando a 100%, os suplementos mais utilizados pela amostra foram: Whey Protein e Maltodextrina. ABSTRACTNutritional profile and food supplement intake of football athletes in a professional club of São Luís-MAProper nutrition can not compensate lack of skill or poor physical conditioning, but can help the player to potentiate his ability and performance. A well-nourished athlete earns more, recovers faster, and gets better results. The present study aims to evaluate the nutritional profile and consumption of athlete supplements in a football club in São Luís-MA. The study population consisted of 26 male athletes from 18 to 35 years of age, but only 21 athletes were recruited according to the selection criteria. For the data collection, the 24h recall was used to measure the total body mass and stature. The scale was used with Weli - W300 model. The variables were evaluated using BioEstat 5.0 software. To define the nutritional profile, the Avanutri 9.0 Software was used. The athletes had an age of 25.67 ± 3.83 years, height of 1.76 ± 0.08 meters; Body mass of 74.86 ± 6.53 kg; BMI of 24.07 ± 1.08 kg / m, the athletes presented a deficit in the proportion between total energy expenditure and the energy value. Total Energy Expenditure of 3,574.35 ± 505.99 Kcal; Total Energy Value of 2,926.86 ± 420.59 Kcal. The consumption of food supplements is significant reaching 100%, the supplements most used by the sample were: Whey Protein and Maltodextrin

Fatal Brazilian spotted fever in a healthy military man during field training in Rio de Janeiro city, southeastern Brazil

Brazilian spotted fever, a zoonotic disease transmitted by ticks, is caused by Rickettsia rickettsii. We report a fulminant case of this zoonosis in a healthy 46-year-old military man in the urban region of Rio de Janeiro city, in October, 2021. Ticks and capybaras (Amblyomma sculptum, Hydrochoerus hydrochaeris, respectively) were identified in the military fields, pointing to the participation of this large synanthropic rodent, recognized as an efficient amplifier host of Rickettsia rickettsii in Brazil. As the military population is considered a risk group for spotted fever, it is necessary to alert health professionals to the importance of the early detection of the disease and its adequate management, mainly in populations that are particularly at risk of exposure to ticks, in order to avoid fatal outcomes

Accuracy of saliva for SARS-CoV-2 detection in outpatients and their household contacts during the circulation of the Omicron variant of concern.

BACKGROUND: While nasopharyngeal (NP) swabs are considered the gold standard for severe acute respiratory coronavirus 2 (SARS-CoV-2) real-time reverse transcriptase-polymerase chain reaction (RT-PCR) detection, several studies have shown that saliva is an alternative specimen for COVID-19 diagnosis and screening. METHODS: To analyze the utility of saliva for the diagnosis of COVID-19 during the circulation of the Omicron variant, participants were enrolled in an ongoing cohort designed to assess the natural history of SARS-CoV-2 infection in adults and children. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and Cohen's kappa coefficient were calculated to assess diagnostic performance. RESULTS: Overall, 818 samples were collected from 365 outpatients from January 3 to February 2, 2022. The median age was 32.8 years (range: 3-94 years). RT-PCR for SARS-CoV-2 was confirmed in 97/121 symptomatic patients (80.2%) and 62/244 (25.4%) asymptomatic patients. Substantial agreement between saliva and combined nasopharyngeal/oropharyngeal samples was observed with a Cohen's kappa value of 0.74 [95% confidence interval (CI): 0.67-0.81]. Sensitivity was 77% (95% CI: 70.9-82.2), specificity 95% (95% CI: 91.9-97), PPV 89.8% (95% CI: 83.1-94.4), NPV 87.9% (95% CI: 83.6-91.5), and accuracy 88.5% (95% CI: 85.0-91.4). Sensitivity was higher among samples collected from symptomatic children aged three years and older and adolescents [84% (95% CI: 70.5-92)] with a Cohen's kappa value of 0.63 (95% CI: 0.35-0.91). CONCLUSIONS: Saliva is a reliable fluid for detecting SARS-CoV-2, especially in symptomatic children and adolescents during the circulation of the Omicron variant

Oral manifestations of COVID-19 in unvaccinated patients: a cross-sectional study

Abstract Background Early studies have highlighted the possible development of dysgeusia and anosmia in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and these manifestations should be considered a potential indication of coronavirus disease 19 (COVID-19). As potential contributors to these symptoms, dentists should perform careful oral and oropharyngeal examinations and document suspicious oral lesions in patients with COVID-19, especially in those who complain of loss of taste and smell. The study’s objective was to assess the prevalence of oral manifestations among ambulatory unvaccinated symptomatic patients with suspected COVID-19 during the acute phase of the disease. Methods This cross-sectional study evaluated oral manifestations in adults (aged ≥ 18 years) with suspected and confirmed SARS-CoV-2 infection. Chi-square and Fisher’s exact tests were used to compare data between the groups (rRT-PCR-positive and rRT-PCR-negative patients). Results One hundred thirty-six participants were included. Most were female (n = 79; 58.1%), with a mean age of 39.53 (± 14.17) years. Of these, 54 (39.7%) had a positive rRT-PCR test, and 82 (60.3%) had negative rRT-PCR results. Oral manifestations were observed in 40 participants (74.1%) in the rRT-PCR-positive group and 67 participants (81.7%) in the rRT-PCR-negative group. The most common oral manifestations were xerostomia (n = 85; 62.5%) and dysgeusia/ageusia (n = 57; 41.9%). Different rates of gingivitis (n = 12; 22.2% vs. n = 5; 6.1%; p = 0.005) and halitosis (n = 7; 13.0% vs. n = 1; 1.2%; p = 0.007) were observed between the rRT-PCR-positive and -negative groups, respectively. Mouth ulcers, glossitis, tongue coating, and petechiae were reported in both groups without significant differences. Conclusions A high prevalence of oral manifestations was observed in symptomatic patients with suspected or confirmed COVID-19. Clinical Relevance This study highlights the importance of routine oral examinations by dentists as part of the multidisciplinary care of COVID-19 patients

Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis

Submitted by Nuzia Santos ([email protected]) on 2015-03-02T11:47:49Z No. of bitstreams: 1 2014_117.pdf: 707401 bytes, checksum: 0f71bb7341baea2f96798e66b6aaca36 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2015-03-02T11:47:57Z (GMT) No. of bitstreams: 1 2014_117.pdf: 707401 bytes, checksum: 0f71bb7341baea2f96798e66b6aaca36 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2015-03-02T11:52:54Z (GMT) No. of bitstreams: 1 2014_117.pdf: 707401 bytes, checksum: 0f71bb7341baea2f96798e66b6aaca36 (MD5)Made available in DSpace on 2015-03-02T11:52:54Z (GMT). No. of bitstreams: 1 2014_117.pdf: 707401 bytes, checksum: 0f71bb7341baea2f96798e66b6aaca36 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofisica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento Clinico e Cirurgico Veterinario. Laboratorio de Patologia e Toxicologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.With the purpose of discovery leishmanicidal secondary metabolites from natural products, crude ethanolic extract (EE) from stems of the Caesalpinia echinata was assayed to verify its in vitro leishmanicidal activity. The EE showed in vitro growth inhibition activities of 90% against amastigote-like of Leishmania (Leishmania) amazonensis. The EE was then submitted to fractionation by Gel Permeation Chromatography (GPC) yielding fifteen fractions (F1 to F15). The same biological assay was performed for the fifteen fractions and the fractions F9 to F11 showed in vitro growth inhibition activities around 80%. The fractions F9 to F11 were pooled to produce an enriched fraction named EF. Evaluation of the acute toxicity of the EE and EF were carried out with Swiss-Webster mice, orally treated by a single oral dose of 300mg of the samples (EE and EF)/kg of body to verify changes in hematological and biochemical profiles and 5.0g of the samples (EE and EF)/kg body to verify the toxicity and safety in using EE and EF as therapeutic agents in the treatment of the leishmaniasis. After preliminary results, the LD50 concentration was estimated to be greater than 5.0g/kg body for both samples (EE and EF) by oral route. The EE and EF of the C. echinata were actives in vitro experiments and nontoxic for mice, moreover these experiments proved to be the first steps towards the development of leishmanicidal agents from C. echinata

Perfil nutricional e consumo de suplemento alimentar de atletas de futebol em um clube profissional de São Luís-MA

Proper nutrition can not compensate lack of skill or poor physical conditioning, but can help the player to potentiate his ability and performance. A well-nourished athlete earns more, recovers faster, and gets better results. The present study aims to evaluate the nutritional profile and consumption of athlete supplements in a football club in São Luís-MA. The study population consisted of 26 male athletes from 18 to 35 years of age, but only 21 athletes were recruited according to the selection criteria. For the data collection, the 24h recall was used to measure the total body mass and stature. The scale was used with Weli - W300 model. The variables were evaluated using BioEstat 5.0 software. To define the nutritional profile, the Avanutri 9.0 Software was used. The athletes had an age of 25.67 ± 3.83 years, height of 1.76 ± 0.08 meters; Body mass of 74.86 ± 6.53 kg; BMI of 24.07 ± 1.08 kg / m, the athletes presented a deficit in the proportion between total energy expenditure and the energy value. Total Energy Expenditure of 3,574.35 ± 505.99 Kcal; Total Energy Value of 2,926.86 ± 420.59 Kcal. The consumption of food supplements is significant reaching 100%, the supplements most used by the sample were: Whey Protein and Maltodextrin.A nutrição adequada não pode compensar a falta de habilidade ou condicionamento físico deficiente, mas pode ajudar o jogador a potencializar sua habilidade e rendimento. Um atleta bem nutrido rende mais, se recupera mais rápido e consegue melhores resultados. O presente estudo tem como objetivo avaliar o perfil nutricional e o consumo de suplementos de atletas em um clube de futebol de São Luís-MA. A população do estudo foi constituída por 26 atletas do naipe masculino com idade entre 18 a 35 anos, entretanto apenas 21 atletas foram recrutados de acordo com os critérios de seleção. Para coleta de dados fora aplicado o recordatório de 24h, para a mensuração da massa corporal total e a estatura foi utilizada a balança com estadiomêtro (Welmy – modelo W300). As variáveis foram avaliadas através do software BioEstat 5.0. Para definição do o perfil nutricional foi utilizado o Software Avanutri 9.0. Os atletas apresentaram idade de 25,67 ±3,83 anos, estatura de 1,76± 0,08 metros; massa corporal de 74,86 ± 6,53 Kg; IMC de 24,07 ± 1,08 Kg/ m, os atletas apresentaram um déficit na proporção entre gasto energético total e o valor energético Gasto Energético total de 3.574,35 ± 505,99 Kcal; Valor Energético Total de 2.926,86 ± 420,59 Kcal. O consumo de suplementos alimentares é significante chegando a 100%, os suplementos mais utilizados pela amostra foram: Whey Protein e Maltodextrina