Two titans finally meet each other under nitrogen deficiencies: FERONIA-TORC1 activation promotes plant growth

Plant growth is determined by well-defined developmental processes that integrate cell-intrinsic factors and external environmental cues, and it is largely dependent on the assimilation of macro- and micro-nutrients from the environment. Between the macro-nutrients, the inorganic nitrogen (e.g., nitrate and ammonium) and amino acids are essential for plant survival and productivity. Beyond acting as macro-nutrients and structural components of macro-molecules, these nitrogen-containing molecules could also act as signaling molecules to orchestrate diverse genetic programs (Wang et al., 2018). Plant nutritional cues that rapidly change over time and space in the soils are tightly linked to signaling pathways that execute fast cellular programs to adjust to a challenging environment. Plant Rapid Alkalinization Factors (RALFs) are secreted peptides that function as extracellular signals and bind to Catharanthus roseus receptor-like kinase 1-like family members such as FERONIA (FER) (Liao et al., 2017). RALF1–FER complexes are central regulators of plant growth that allow plants to respond to environmental changes (Du et al., 2016; Zhu et al., 2020). This interaction triggers the recruitment of RPM1-induced protein kinase (RIPK) and the phosphorylation of both FER and RIPK in a mutually dependent manner (Du et al., 2016), followed by the recruitment and activation via phosphorylation of an early translation initiation factor (eIF4E1) (Zhu et al., 2020). These findings highlighted that the RALF1–FER–RIPK pathway is an important hub to control plant cell growth under specific conditions.Fil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Andrés Bello; Chile. Millennium Nucleus for the Development of Super Adaptable Plants; Chil

Autocrine regulation of root hair size by the RALF-FERONIA-RSL4 signaling pathway

Root hair (RH) size has vital physiological implications, since it influences the surface area of the root and thus the ability of the plant to absorb water and nutrients from the soil. Arabidopsis ROOT HAIR DEFECTIVE 6-LIKE 4 (RSL4), a bHLH transcription factor, controls the expression of hundreds of RH genes, and RSL4 expression itself can trigger ectopic RH growth. Recent studies reveal an autocrine mechanism governing plant RH cell growth in which the extracellular peptide RAPID ALKALINIZATION FACTOR 1 (RALF1) and receptor FERONIA (FER) act as a central hub between the cell surface and downstream signaling events. RALF1-FER promotes the phosphorylation of eIF4E1. Then, phosphorylated eIF4E1 further regulates the synthesis of RH proteins, including RSL4, to promote RH growth. High levels of RSL4 exert a negative feedback on RALF1 expression via directly binding to the RALF1 gene promoter, slowing RH growth and determining final RH cell size.Fil: Zhu, Sirui. Hunan University; ChinaFil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Estevez, Jose Manuel. Universidad Andrés Bello; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Yu, Feng. State Key Laboratory Of Hybrid Rice; China. Hunan University; Chin

Potato peels waste as a sustainable source for biotechnological production of biofuels: Process optimization

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGPotato peel waste (PPW) is a starchy by-product generated in great amounts during the industrial processing of potatoes. It can be used as a low cost alternative, and renewable feedstock for the production of second generation bioethanol. In order to intensify this process, Saccharomyces cerevisiae Ethanol Red®, a robust and thermotolerant yeast strain, was selected and two experimental designs and response surfaces assessment were conducted to enable very high gravity fermentations (VHGF) using PPW as feedstock. The first one focused on the optimization of the liquefaction and enzymatic hydrolysis stages, enabling a maximum ethanol concentration of 116.5 g/L and a yield of 80.4 % at 72 h of fermentation; whereas, the second one, focus on the optimization of the pre-saccharification and fermentation stages, which further increased process productivity, leading to a maximum ethanol concentration of 108.8 g/L and a yield of 75.1 % after 54 h of fermentation. These results allowed the definition of an intensified pre-saccharification and simultaneous saccharification and fermentation (PSSF) process for ethanol production from PPW, resorting to short liquefaction and presaccharification times, 2 h and 10 h respectively, at an enzyme loading of 80 U/g PPW of Viscozyme and 5 UE/g PPW of SAN Super and a higher fermentation temperature of 34 ◦C due to the use of a thermotolerant yeast. Overall, with these conditions and solely from PPW without any supplementation, the outlined PSSF process allowed reaching a high ethanol concentration and yield (104.1 g/L and 71.9 %, respectively) standing at high productivities with only 54 h of fermentation.Xunta de Galicia | Ref. ED431F 2020/03Xunta de Galicia | Ref. GRC ED431C 2022/08Xunta de Galicia | Ref. GRC ED431C 2021/46Ministerio de Economía y Competitividad | Ref. PID2020-116717RB-I00Ministerio de Economía y Competitividad | Ref. PID2019- 110031RB-I00Ministerio de Economía y Competitividad | Ref. RYC2018-026177-IXunta de Galicia | Ref. UIDB/ 04469/2020Fundação para a Ciência e a Tecnologia | Ref. UIDB/ 04469/202

A comparison of Clinical Risk Index for babies (CRIB-II), Score for Neonatal Acute Physiology (SNAP-II) and SNAPPE-II in predicting parenteral nutrition necessity in low birth weight preterm neonates.

Advances in perinatal care have made it possible to improve survival of low birth weight neonates. Clinical risk index for babies (CRIB-II), score for neonatal acute physiology (SNAP-II), and SNAP-perinatal extension-II (SNAPPE-II) have been used as mortality predictors for preterm infants. Feeding intolerance is very frequent in preterm neonates, and the development of an early effective biomarker for its prediction could be useful for carrying out a proper feeding strategy. Our aim was to compare the ability of CRIB-II, SNAP-II and SNAPPE-II in predict the feeding intolerance and parenteral nutrition necessity in preterm neonates. Methods: A retrospective cohort study on preterm neonates’ born at Jaen Hospital Complex with low birth weight and ≤ 36 weeks of gestation was done. Epidemiological, clinical and clinical scores CRIB II, SNAP-II and SNAPPE-II were recorded. Results: 255 low birth weight preterm neonates, 131 males (51.4%), aged ≤32 weeks of gestation (71%), were enrolled at our hospital. Parenteral nutrition needed were significantly higher in preterm neonates weighed 2500-1500 g (73.3%) and ≤ 1000g (87%). CRIB-II, SNAP-II and SNAPPE-II mean values were higher in neonates group subjected to parenteral nutrition compared with oral nutrition (p<0.05). CRIB-II and SNAPPE-II scores significantly correlated with parenteral nutrition days (p<0.05). Overall mortality rate was 11%. The 78.6% of all deceased infants needed parenteral nutrition. Conclusion: Clinical Risk Index for babies (CRIB-II) better than SNAPPE-II correlated with the feeding intolerance and thus the parenteral nutrition days in preterm neonates with low birth weight.Subvencionado: Ayuda del Plan Propio de Investigación de la UMA. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

A DEM approach for simulating flexible beam elements with the Project Chrono core module in DualSPHysics

This work presents a novel approach for simulating elastic beam elements in DualSPHysics leveraging functions made available by the coupling with the Project Chrono library. Such numerical frameworks, belonging to the Meshfree Particle Methods family, stand out for several features, like complex multiphase phenomena, moving boundaries, and high deformations which are handled with relative ease and reasonable numerical stability and reliability. Based on a co-rotating rigid element structure and lumped elasticity, a cogent mathematical formulation, relying on the Euler–Bernoulli beam theory for the structural discretization, is presented and applied to simulating two-dimensional flexible beams with the discrete elements method (DEM) formulation. Three test cases are presented to validate the smoothed particle hydrodynamics-based (SPH) structure model in both accuracy and stability, starting from an equilibrium test, to the dynamic response, and closing with a fluid–structure interaction simulation. This work proves that the developed theory can be used within a Lagrangian framework, using the features provided by a DEM solver, overtaking the initial limitations, and hence applying the results of static theories to complex dynamic problems.Xunta de Galicia | Ref. ED431C 2021/44Xunta de Galicia | Ref. ED481A-2021/337Ministerio de Ciencia, Innovación y Universidades | Ref. IJCI-2017-32592Agencia Estatal de Investigación | Ref. PID2020-113245RB-I0

Regular wave seakeeping analysis of a planing hull by smoothed particle hydrodynamics: a comprehensive validation

In this work, the dynamics of a planing hull in regular head waves was investigated using the Smoothed Particle Hydrodynamics (SPH) meshfree method. The simulation of the interaction of such vessels with wave trains features several challenging characteristics, from the complex physical interaction, due to large dynamic responses, to the likewise heavy numerical workload. A novel numerical wave flume implemented within the SPH-based code DualSPHysics fulfills both demands, guaranteeing comparable accuracy with an established proprietary Computational Fluid Dynamics (CFD) solver without sharpening the computational load. The numerical wave flume uses ad hoc open-boundary conditions to reproduce the flow characteristics encountered by the hull during its motion, combining the current and waves while adjusting their properties with respect to the vessel’s experimental towing speed. It follows a relatively small three-dimensional domain, where the potentiality of the SPH method in modeling free-surface flows interacting with moving structures is unleashed. The results in different wave conditions show the feasibility of this novel approach, considering the overall good agreement with the experiments; hence, an interesting alternative procedure to simulate the seakeeping test in several marine conditions with bearable effort and satisfying accuracy is established.Ministerio de Ciencia e Innovación | Ref. PID2020-113245RBI00Xunta de Galicia | Ref. ED431C 2021/44Ministerio de Ciencia e Innovación | Ref. TED2021-129479AI00Xunta de Galicia | Ref. ED481A-2021/337Ministerio de Ciencia e Innovación | Ref. RYC2020-030197-

The lncRNA APOLO and the transcription factor WRKY42 target common cell wall EXTENSIN encoding genes to trigger root hair cell elongation

Plant long noncoding RNAs (lncRNAs) are key chromatin dynamics regulators, directing the transcriptional programs driving a wide variety of developmental outputs. Recently, we uncovered how the lncRNA AUXIN REGULATED PROMOTER LOOP (APOLO) directly recognizes the locus encoding the root hair (RH) master regulator ROOT HAIR DEFECTIVE 6 (RHD6) modulating its transcriptional activation and leading to low temperature-induced RH elongation. We further demonstrated that APOLO interacts with the transcription factor WRKY42 in a novel ribonucleoprotein complex shaping RHD6 epigenetic environment and integrating signals governing RH growth and development. In this work, we expand this model showing that APOLO is able to bind and positively control the expression of several cell wall EXTENSIN (EXT) encoding genes, including EXT3, a key regulator for RH growth. Interestingly, EXT3 emerged as a novel common target of APOLO and WRKY42. Furthermore, we showed that the ROS homeostasis-related gene NADPH OXIDASE C (NOXC) is deregulated upon APOLO overexpression, likely through the RHD6-RSL4 pathway, and that NOXC is required for low temperature-dependent enhancement of RH growth. Collectively, our results uncover an intricate regulatory network involving the APOLO/WRKY42 hub in the control of master and effector genes during RH development.Fil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Mansilla, Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Moison, Michael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Berdion Gabarain, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Andrés Bello; Chile. Millennium Institute for Integrative Biology; Chil

Coupling an SPH-based solver with an FEA structural solver to simulate free surface flows interacting with flexible structures

This work proposes a two-way coupling between a Smoothed Particle Hydrodynamics (SPH) model-based named DualSPHysics and a Finite Element Analysis (FEA) method to solve fluid–structure interaction (FSI). Aiming at having a computationally efficient solution via spatial adjustable resolutions for the two phases, the SPH-FEA coupling herein presented implements the Euler–Bernoulli beam model, based on a simplified model that incorporates axial and flexural deformations, to introduce a solid solver in the DualSPHysics framework. This approach is particularly functional and very precise for slender beam elements undergoing large displacements, and large deformations can also be experienced by the structural elements due to the non-linear FEA implementation via a co-rotational formulation. In this two-way coupling, the structure is discretised in the SPH domain using boundary particles on which the forces exerted by fluid phases are computed. Such forces are passed over to the FEA structural solver that updates the beam shape and, finally, the particle positions are subsequently reshuffled to represent the deformed shape at each time step. The SPH-FEA coupling is validated against four reference cases, which prove the model to be as accurate as other approaches presented in literature.Ministerio de Ciencia e Innovación | Ref. PID2020-113245RB-I00Ministerio de Ciencia e Innovación | Ref. TED2021-129479A-I00Xunta de Galicia | Ref. ED431C 2021/44Xunta de Galicia | Ref. ED481A-2021/337Universidade de Vigo/CISU

Coupling of an SPH-based solver with a multiphysics library

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGA two-way coupling between the Smoothed Particle Hydrodynamics-based (SPH) code with a multiphysics library to solve complex fluid-solid interaction problems is proposed. This work provides full access to the package for the use of this coupling by releasing the source code, completed with guidelines for its compilation and utilization, and self-contained template setups for practical uses of the novel implemented features, is provided here. The presented coupling expands the applicability of two different solvers allowing to simulate fluids, multibody systems, collisions with frictional contacts using either non-smooth contact (NSC) or smooth contact (SMC) methods, all integrated under the same framework. The fluid solver is the open-source code DualSPHysics, highly optimised for simulating free-surface phenomena and structure interactions, uniquely positioned as a general-purpose Computational Fluid Dynamics (CFD) software with a GPU-accelerated solver. Mechanical systems that comprise collision detection and/or multibody dynamics are solved by the multiphysics library Project Chrono, which uses a Discrete Element Method (DEM). Therefore, this SPH-DEM coupling approach can manage interactions between fluid and complex multibody systems with relative constraints, springs, or mechanical joints.Ministerio de Ciencia e Innovación | Ref. PID2020-113245RB-I00Xunta de Galicia | Ref. ED431C 2021/44Xunta de Galicia | Ref. ED481A-2021/337Ministerio de Ciencia e Innovación, Xunta de Galicia con fondos de la Unión Europea NextGenerationEU y el Fondo Europeo Marítimo y de Pesca | Ref. PRTR-C17.I

THE HIGH CADENCE TRANSIENT SURVEY (HITS). I. SURVEY DESIGN AND SUPERNOVA SHOCK BREAKOUT CONSTRAINTS

Indexación: Web of Science; Scopus.We present the first results of the High Cadence Transient Survey (HiTS), a survey for which the objective is to detect and follow-up optical transients with characteristic timescales from hours to days, especially the earliest hours of supernova (SN) explosions. HiTS uses the Dark Energy Camera and a custom pipeline for image subtraction, candidate filtering and candidate visualization, which runs in real-time to be able to react rapidly to the new transients. We discuss the survey design, the technical challenges associated with the real-time analysis of these large volumes of data and our first results. In our 2013, 2014, and 2015 campaigns, we detected more than 120 young SN candidates, but we did not find a clear signature from the short-lived SN shock breakouts (SBOs) originating after the core collapse of red supergiant stars, which was the initial science aim of this survey. Using the empirical distribution of limiting magnitudes from our observational campaigns, we measured the expected recovery fraction of randomly injected SN light curves, which included SBO optical peaks produced with models from Tominaga et al. (2011) and Nakar & Sari (2010). From this analysis, we cannot rule out the models from Tominaga et al. (2011) under any reasonable distributions of progenitor masses, but we can marginally rule out the brighter and longer-lived SBO models from Nakar & Sari (2010) under our best-guess distribution of progenitor masses. Finally, we highlight the implications of this work for future massive data sets produced by astronomical observatories, such as LSST.http://iopscience.iop.org/article/10.3847/0004-637X/832/2/155/meta;jsessionid=76BDFFFE378003616F6DBA56A9225673.c4.iopscience.cld.iop.or