Acceptability and Feasibility of Web-based Diabetes Instruction for Latinos with Limited Education and Computer Experience

Introduction: The internet offers an important avenue for developing diabetes self-management skills, but many Latinos have limited experience with computer-based instruction. Objective: To evaluate the feasibility and acceptability of delivering a web-based diabetes education program in a computer classroom for Spanish-speaking Latinos. Methods: Spanish-speaking Latinos (n=26) attended two classroom sessions to learn computer skills while navigating a web-based diabetes education platform. Diabetes knowledge was assessed before and after the intervention; structured interviews were completed to assess program acceptability. Results: Half of participants (50%) had not previously used a computer. Post-intervention, diabetes knowledge improved significantly (p=.001). The majority of participants (86%) indicated a preference for web-based instruction as a stand-alone program or as an adjunct to traditional classroom training, particularly citing the advantage of being able to engage the material at their own pace. Conclusion: With limited support, Latinos with minimal computer experience can effectively engage in web-based diabetes education

Impacto del cambio climático sobre la distribución de Prosopis Hassleri y P.alba en la región chaqueña

El algarrobo, principalmente Prosopis alba y P. hassleri son las especies nativas de mayor uso para madera de aserrío en nuestro país, registrándose en los últimos años un aumento constante en la tasa de consumo. Dado que la totalidad de la madera de algarrobo consumida proviene de bosques nativos, la explotación del recurso es insostenible no sólo desde el punto de vista ambiental sino también económico. A esta situación crítica se le agregan además problemáticas que están afectando a los bosques a escala global como es la expansión de la frontera agrícola sobre áreas de bosque nativo, forestación con especies exóticas de crecimiento rápido, y la interacción de estos factores con el cambio climático actual. En este trabajo indagamos sobre el posible impacto del cambio climático sobre la distribución de P. hassleri (Algarrobo paraguayo) y de tres morfotipos de P. alba (santiagueño, chaqueño y chaqueño sur) en el norte de Argentina, para proveer información útil en la planificación de estrategias de conservación y manejo de los recursos genéticos nativos. Para ello, se utilizaron modelos predictivos de nicho que permitieron estimar la distribución potencial de ambas especies bajo un escenario actual y de cambio climático futuro. De esta manera se pudieron establecer áreas de alto y bajo impacto, y nuevas áreas adecuadas para la persistencia de las respectivas especies. Se utilizaron datos georreferenciados de 30-77 individuos de poblaciones naturales de cada una de las especies y morfotipos, y los 19 parámetros bioclimáticos disponibles en la base de datos mundial WorldClim. Los análisis de modelado de nicho actual y futuro se realizaron mediante el algoritmo de máxima entropía implementado en el programa Maxent; posteriormente los modelados resultantes fueron procesados en el programa DIVA-GIS. Los resultados indican que todas las especies/morfotipos tienden a desplazar su área de distribución hacia el sur, excepto el morfotipo P. alba chaqueño sur que se expandiría hacia el norte. Prosopis hassleri sería la especie que perdería la mayor proporción de superficie de su distribución actual. Sin embargo, en un escenario futuro surgirían áreas favorables para su persistencia cuya superficie duplicaría la distribución actual. Contrariamente, el morfotipo santiagueño de P. alba conservaría casi la totalidad de su área de distribución actual, y es la que menos ampliaría su rango de distribución en un escenario futuro. A partir de los resultados obtenidos se sugieren estrategias de manejo y conservación para estas especies, a través de la identificación y protección de refugios climáticos y del establecimiento de corredores biológicos que favorezcan la dispersión natural de las especies hacia nuevas áreas adecuadas. (Nuestro agradecimiento al Proyecto Específico INTA PNFOR044341 y al Banco Nacional de Germoplasma de Prosopis, FCA-UNC, por facilitar la información básica para este trabajo).Fil: Venier, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cosacov Martinez, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: López Lauenstein, D.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; ArgentinaFil: Vega, C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; ArgentinaFil: Verga, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentin

The Nature of the Secondary Star in the Black Hole X-Ray Transient V616 Mon (=A0620-00)

We have used NIRSPEC on Keck II to obtain $K$-band spectroscopy of the low mass X-ray binary V616 Mon (= A0620$-$00). V616 Mon is the proto-typical soft x-ray transient containing a black hole primary. As such it is important to constrain the masses of the binary components. The modeling of the infrared observations of ellipsoidal variations in this system lead to a derived mass of 11.0 M_{\sun} for the black hole. The validity of this derivation has been called into question due to the possiblity that the secondary star's spectral energy distribution is contaminated by accretion disk emission (acting to dilute the variations). Our new $K$-band spectrum of V616 Mon reveals a late-type K dwarf secondary star, but one that has very weak $^{\rm 12}$CO absorption features. Comparison of V616 Mon with SS Cyg leads us to estimate that the accretion disk supplies only a small amount of $K$-band flux, and the ellipsoidal variations are not seriously contaminated. If true, the derived orbital inclination of V616 Mon is not greatly altered, and the mass of the black hole remains large. A preliminary stellar atmosphere model for the $K$-band spectrum of V616 Mon reveals that the carbon abundance is approximately 50% of the solar value. We conclude that the secondary star in V616 Mon has either suffered serious contamination from the accretion of supernova ejecta that created the black hole primary, or it is the stripped remains of a formerly more massive secondary star, one in which the CNO cycle had been active.Comment: 20 pages, 5 figure

Age constraints on the dispersal of dinosaurs in the Late Triassic from magnetochronology of the Los Colorados Formation (Argentina)

A measured magnetozone sequence defined by 24 sampling sites with normal polarity and 28 sites with reverse polarity characteristic magnetizations was established for the heretofore poorly age-constrained Los Colorados Formation and its dinosaur-bearing vertebrate fauna in the Ischigualasto–Villa Union continental rift basin of Argentina. The polarity pattern in this ∼600-m-thick red-bed section can be correlated to Chrons E7r to E15n of the Newark astrochronological polarity time scale. This represents a time interval from 227 to 213 Ma, indicating that the Los Colorados Formation is predominantly Norian in age, ending more than 11 My before the onset of the Jurassic. The magnetochronology confirms that the underlying Ischigualasto Formation and its vertebrate assemblages including some of the earliest known dinosaurs are of Carnian age. The oldest dated occurrences of vertebrate assemblages with dinosaurs in North America (Chinle Formation) are younger (Norian), and thus the rise of dinosaurs was diachronous across the Americas. Paleogeography of the Ischigualasto and Los Colorados Formations indicates prolonged residence in the austral temperate humid belt where a provincial vertebrate fauna with early dinosaurs may have incubated. Faunal dispersal across the Pangean supercontinent in the development of more cosmopolitan vertebrate assemblages later in the Norian may have been in response to reduced contrasts between climate zones and lowered barriers resulting from decreasing atmospheric pCO2 levels

Leptin Inhibits Angiotensin II-Induced Intracellular Calcium Increase and Vasoconstriction in the Rat Aorta

Besides its role in body weight control leptin may also act as a vasoactive hormone. This study was designed to investigate whether leptin modifies angiotensin II (ANG II)-induced vascular responses. The expression of functional leptin receptors (OB-Rb) was detected in vascular smooth muscle cells (VSMCs) from adult Wistar rats by RT-PCR. Immunocytochemistry and Western blot analysis further showed the expression of OB-R protein in VSMCs. The ANG II (10(-7) mol/liter)-induced increase in intracellular Ca(2+) was blocked (P < 0.01) by leptin (10(-8) mol/liter). Moreover, in calcium-free buffer leptin was able to inhibit 65% of the ANG II-induced calcium release from intracellular stores. In endothelium-denuded aortic rings from adult Wistar rats no effect of leptin on basal tension was observed. However, the ANG II-induced isometric contraction was reduced (P < 0.05) by leptin (10(-8) mol/liter). The experiments were also performed in age- and sex-matched Zucker rats, in which no effect of leptin on ANG II-induced calcium increase and vasoconstriction was observed. It is concluded that leptin blocks the vasoconstrictor action of ANG II and inhibits the ANG II-induced increase in intracellular Ca(2+) in VSMCs through OB-Rb. These findings provide new insight into the physiological effects of leptin on blood pressure regulation

Isolation and characterization of <i>Magnetospirillum</i> sp strain 15-1 as a representative anaerobic toluene-degrader from a constructed wetland model

Previously, Planted Fixed-Bed Reactors (PFRs) have been used to investigate microbial toluene removal in the rhizosphere of constructed wetlands. Aerobic toluene degradation was predominant in these model systems although bulk redox conditions were hypoxic to anoxic. However, culture-independent approaches indicated also that microbes capable of anaerobic toluene degradation were abundant. Therefore, we aimed at isolating anaerobic-toluene degraders from one of these PFRs. From the obtained colonies which consisted of spirilli-shaped bacteria, a strain designated 15-1 was selected for further investigations. Analysis of its 16S rRNA gene revealed greatest similarity (99%) with toluene-degrading Magnetospirillum sp. TS-6. Isolate 15-1 grew with up to 0.5 mM of toluene under nitrate-reducing conditions. Cells reacted to higher concentrations of toluene by an increase in the degree of saturation of their membrane fatty acids. Strain 15-1 contained key genes for the anaerobic degradation of toluene via benzylsuccinate and subsequently the benzoyl-CoA pathway, namely bssA, encoding for the alpha subunit of benzylsuccinate synthase, bcrC for subunit C of benzoyl-CoA reductase and bamA for 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase. Finally, most members of a clone library of bssA generated from the PFR had highest similarity to bssA from strain 15-1. Our study provides insights about the physiological capacities of a strain of Magnetospirillum isolated from a planted system where active rhizoremediation of toluene is taking place

Characterization of Epstein Barr Virus Latency Pattern in Argentine Breast Carcinoma

INTRODUCTION: Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV latency pattern in our EBV positive breast carcinoma series. METHODS: The study was conducted on 71 biopsies of breast carcinoma and in 48 non-neoplastic breast controls. EBNA1, LMP2A and LMP1 expression was assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA and EBERs RNA transcripts expression was performed by in situ hybridization. EBV presence was confirmed by PCR. RESULTS: EBV genomic DNA and EBNA1 expression were detected in 31% (22/71) of patients specifically restricted to tumor epithelial cells in breast carcinoma while all breast control samples were negative for both viral DNA and EBNA1 protein. LMP2A was detected in 73% of EBNA1 positive cases, none of which expressed either LMP1 protein or EBERs transcripts. CONCLUSIONS: These findings suggest that EBV expression pattern in the studied biopsies could be different from those previously observed in breast carcinoma cell lines and lead us to suggest a new, EBNA1, LMP2A positive and LMP1 and EBERs negative latency profile in breast carcinoma in our population

Laboratory animals and respiratory allergies: The prevalence of allergies among laboratory animal workers and the need for prophylaxis

OBJECTIVE: Subjects exposed to laboratory animals are at a heightened risk of developing respiratory and allergic diseases. These diseases can be prevented by simple measures such as the use of personal protective equipment. We report here the primary findings of the Laboratory Animals and Respiratory Allergies Study regarding the prevalence of allergic diseases among laboratory animal workers, the routine use of preventive measures in laboratories and animal facilities, and the need for prevention programs. METHODS: Animal handlers and non-animal handlers from 2 Brazilian universities (University of São Paulo and State University of Campinas) answered specific questionnaires to assess work conditions and symptoms. These subjects also underwent spirometry, a bronchial challenge test with mannitol, and skin prick tests for 11 common allergens and 5 occupational allergens (rat, mouse, guinea pig, hamster, and rabbit). RESULTS: Four hundred fifty-five animal handlers (32±10 years old [mean±SD], 209 men) and 387 non-animal handlers (33±11 years old, 121 men) were evaluated. Sensitization to occupational allergens was higher among animal handlers (16%) than non-animal handlers (3%,

Traditional plants from Asteraceae family as potential candidates for functional food industry

Traditional plants have been used in the treatment of disease and pain due to their beneficial properties such as antioxidant, antiinflammation, analgesic, and antibiotic activities. The Asteraceae family is one of the most common groups of plants used in folk medicine. The species Achillea millefolium, Arnica montana, Bellis perennis, Calendula officinalis, Chamaemelum nobile, Eupatorium cannabinum, Helichrysum stoechas, and Taraxacum officinale have been used in different remedies in Northwest Spain. Besides health benefits, some of them like C. nobile and H. stoechas are already employed in cooking and culinary uses, including cocktails, desserts, and savory dishes. This study aimed to review the current information on nutritive and beneficial properties and bioactive compounds of these plants, which are not mainly used as foods but are possible candidates for this purpose. The report highlights their current uses and suitability for the development of new functional food industrial applications. Phenolic compounds, essential oils, and sesquiterpene lactones are some of the most important compounds, being related to different bioactivities. Hence, they could be interesting for the development of new functional foods.The research leading to these results received institutioanl and financial support from the: Programa de Cooperación Interreg V-A España—Portugal (POCTEP) 2014–2020 (Projects Ref.: 0181_NANOEATERS_01_E and 0377_IBERPHENOL_6_E); MICINN supporting the Ramón&Cajal grant for M. A. Prieto (RYC-2017-22891); Xunta de Galicia and University of Vigo for supporting the post-doctoral grant of María Fraga Corral (ED481B-2019/096) and the pre-doctoral grant of P. García- Oliveira (ED481A-2019/295); to Xunta de Galicia for the program EXCELENCIA-ED431F 2020/12; to Ibero-American Program on Science and Technology (CYTED – AQUA-CIBUS, P317RT0003); by EcoChestnut Project (Erasmus+ KA202) that supports the work of M. Carpena; by the Bio Based Industries Joint Undertaking (JU) under grant agreement no. 888003 UP4HEALTH Project (H2020-BBI-JTI-2019), the JU receives support from the European Union’s Horizon 2020 Research and Innovation Program and the Bio Based Industries Consortium.info:eu-repo/semantics/publishedVersio

Torasemide inhibits angiotensin II-induced vasoconstriction and intracellular calcium increase in the aorta of spontaneously hypertensive rats

Torasemide is a loop diuretic that is effective at low once-daily doses in the treatment of arterial hypertension. Because its antihypertensive mechanism of action may not be based entirely on the elimination of salt and water from the body, a vasodilator effect of this drug can be considered. In the present study, the ability of different concentrations of torasemide to modify angiotensin II (Ang II)-induced vascular responses was examined, with the use of an organ bath system, in endothelium-denuded aortic rings from spontaneously hypertensive rats. Ang II-induced increases of intracellular free calcium concentration ([Ca(2+)](i)) were also examined by image analysis in cultured vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats. A dose-response curve to Ang II was plotted for cumulative concentrations (from 10(-9) to 10(-6) mol/L) in endothelium-denuded aortic rings (pD(2)=7.5+/-0.3). Isometric contraction induced by a submaximal concentration of Ang II (10(-7) mol/L) was reduced in a dose-dependent way by torasemide (IC(50)=0.5+/-0.04 micromol/L). Incubation of VSMCs with different concentrations of Ang II (from 10(-10) to 10(-6) mol/L) resulted in a dose-dependent rise of [Ca(2+)](i) (pD(2)=7.5+/-0.3). The stimulatory effect of [Ca(2+)](i) induced by a submaximal concentration of Ang II (10(-7) mol/L) was blocked by torasemide (IC(50)=0.5+/-0.3 nmol/L). Our findings suggest that torasemide blocks the vasoconstrictor action of Ang II in vitro. This action can be related to the ability of torasemide to block the increase of [Ca(2+)](i) induced by Ang II in VSMCs. It is proposed that these actions might be involved in the antihypertensive effect of torasemide observed in vivo