TMT-Based Proteomic Analysis of Human Spermatozoa from Unexplained Recurrent Miscarriage Patients before and after Oral Antioxidant Treatment

Recently, sperm quality and the presence of double-stranded breaks (DSB) has been pointed out as a possible cause of recurrent miscarriage, and the use of antioxidants has expanded as a treatment for male infertility. The aim of the present study was to analyze the proteomic effects of antioxidants on sperm from RM patients with high incidence of DSB. Proteomic analysis was performed using a tandem mass tag labeling technique, and subsequently compared with the PANTHER database for DEPs, and the STRING database for protein-protein interactions (PPI). Differentially expressed proteins (DEPs) both before and after antioxidant oral treatment were identified. PPI involving DEPs clustered into networks related to cell metabolism, cytoskeleton, and DNA damage. Results show that the sperm proteomic profiles before and after antioxidant treatment do not significantly differ from each other. However, some DEPs found after the antioxidant treatment shifted towards a DEPs profile typical of fertile donors. This indirect measurement suggests an improvement caused by antioxidants on the expression of several proteins. Among them were proteins involved in sperm DNA remodeling (LMO7, MMP28, BNC2, H2B, and PRDM2). The results presented here represent the first approach in the analysis and repair of the proteomic change caused by antioxidants in recurrent miscarriage patients, elucidating biomarkers that may be useful for the diagnosis and further sperm selection in this type of patient. Further studies should be conducted to validate the usefulness of these biomarkers in larger study groups

Longer breastfeeding duration is associated with lower consumption of ultraprocessed foods in a sample of spanish preschoolers: The SENDO project

Background: Breastfeeding has been linked to a higher consumption of fruit and vegetables at ages 4 to 5 years. More recently, it has been suggested that it may also be associated with lower ultraprocessed food (UPF) consumption in childhood. Objective: The aim of this study was to assess whether breastfeeding duration was associated with consumption of UPF in a sample of Mediterranean preschoolers. Design: This study involved a cross-sectional analysis of baseline information of children in the Child Follow-Up for Optimal Development cohort. Children were enrolled at ages 4 to 5 years and information was gathered through an online questionnaire completed by parents. Dietary information was collected with a previously validated semi-quantitative food frequency questionnaire and foods were classified based on the degree of processing according to the NOVA classification. Participants/setting: This study used baseline information for 806 participants enrolled in the Child Follow-Up for Optimal Development cohort between January 2015 and June 2021 in Spain. Main outcomes measures: Main study outcome measures were difference in grams per day and in the percentage of total energy intake from UPF consumption related to breastfeeding duration, and odds ratio that UPF represents a high percentage of total energy intake. Statistical analyses: Crude and multivariable adjusted estimates were calculated with generalized estimating equations to account for intracluster correlation between siblings. Results: The prevalence of breastfeeding in the sample was 84%. After adjusting for potential confounders, children who were breastfed for some time reported significantly lower consumption of UPF than children who were not breastfed at all. The mean differences were -19.2 g (95% CI -44.2 to 10.8) for children who were breastfed for <6 months, -42.5 g (95% CI -77.2 to -7.80) for those who were breastfed for 6 to 12 months, and -43.6 g (95% CI -79.8 to -7.48) for those who were breastfed for 12 months or more (P value for trend = 0.01). After adjusting for potential confounders, compared with children who were not breastfed, those who were breastfed for ≥12 months had consistently lower odds of UPF representing more than 25%, 30%, 35%, and 40% of total energy intake. Conclusions: Breastfeeding is associated with lower consumption of UPF in Spanish preschoolers

Low-Complexity 3D-DWT video encoder applicable to IPTV

3D-DWT encoders are good candidates for applications like professional video editing, IPTV video surveillance, live event IPTV broadcast, multispectral satellite imaging, HQ video delivery, etc., where a frame must be reconstructed as fast as possible. However, the main drawback of the algorithms that compute the 3D-DWT is the huge memory requirement in practical implementations. In this paper, and in order to considerably reduce the memory requirements of this kind of video encoders, we present a new 3D-DWT video encoder based on (a) the use of a novel frame-based 3D-DWT transform that avoids video sequence partitioning in Groups Of Pictures (GOP) and (b) a very fast run-length encoder. Furthermore, an exhaustive evaluation of the proposed encoder (3D-RLW) has been performed, analyzing the sensibility of the ¿lters employed in the 3D-DWT transform and comparing the evaluation results with other video encoders in terms of R/D, coding/decoding delay and memory consumptionThanks to Spanish Ministry of Education and Science under grants DPI2007-66796-C03-03 for funding.López ., O.; Piñol ., P.; Martinez Rach, MO.; Perez Malumbres, MJ.; Oliver Gil, JS. (2011). Low-Complexity 3D-DWT video encoder applicable to IPTV. Signal Processing: Image Communication. 26(7):358-369. https://doi.org/10.1016/j.image.2011.01.008S35836926

On the Performance of video quality assessment metrics under different compression and packet llss scenariov

[EN] When comparing the performance of video coding approaches, evaluating different commercial video encoders, or measuring the perceived video quality in a wireless environment, Rate/distortion analysis is commonly used, where distortion is usually measured in terms of PSNR values. However, PSNR does not always capture the distortion perceived by a human being. As a consequence, significant efforts have focused on defining an objective video quality metric that is able to assess quality in the same way as a human does. We perform a study of some available objective quality assessment metrics in order to evaluate their behavior in two different scenarios. First, we deal with video sequences compressed by different encoders at different bitrates in order to properly measure the video quality degradation associated with the encoding system. In addition, we evaluate the behavior of the quality metrics when measuring video distortions produced by packet losses in mobile ad hoc network scenarios with variable degrees of network congestion and node mobility. Our purpose is to determine if the analyzed metrics can replace the PSNR while comparing, designing, and evaluating video codec proposals, and, in particular, under video delivery scenarios characterized by bursty and frequent packet losses, such as wireless multihop environments.This research was supported by the Spanish Ministry of Education and Science under Grant no. TIN2011-27543-C0303.S.Martinez-Rach, MO.; Pinol, P.; Lopez, OM.; Perez Malumbres, M.; Oliver Gil, JS.; Tavares De Araujo Cesariny Calafate, CM. (2014). On the Performance of video quality assessment metrics under different compression and packet llss scenariov. Scientific World Journal. 2014:1-18. doi:10.1155/2014/743604S118201

Low-Complexity TTCM Based Distributed Video Coding Architecture

Abstract. Distributed Video Coding (DVC) is a promising coding solution for some emerging applications, where the encoder complexity, power consumption or memory requirements are constraint the system resources. Current approaches to DVC focus on improving the performance of the WynerZiv coding by improving the quality of the reconstructed side information or by improving the quality of channel codes. Up to date, no attention has been paid to the problem of key frames coding where a low-encoding complexity scenario is also needed. This work focuses on key frames coding in its effect to the Wyner-Ziv frames decoding aiming to implement a very low-complexity Turbo Trellis Coded Modulation (TTCM) based DVC architecture. In this paper, we propose a new key frame coding scheme which has very low complexity and memory requirements for the TTCM based distributed video codec. Results show that the proposed intra frame codec for key frame coding outperforms the JPEG2000 and the Intra H.264 AVC codecs in terms of encoding-time and memory requirements, with better RD performance

Measurement of triple gauge boson couplings from WW production at LEP energies up to 189 GeV

A measurement of triple gauge boson couplings is presented, based on W-pair data recorded by the OPAL detector at LEP during 1998 at a centre-of-mass energy of 189 GeV with an integrated luminosity of 183 pb^-1. After combining with our previous measurements at centre-of-mass energies of 161-183 GeV we obtain k_g=0.97 +0.20 -0.16, g_1^z=0.991 +0.060 -0.057 and lambda_g=-0.110 +0.058 -0.055, where the errors include both statistical and systematic uncertainties and each coupling is determined by setting the other two couplings to their SM values. These results are consistent with the Standard Model expectations.Comment: 28 pages, 8 figures, submitted to Eur. Phys. J.

Predicting survival in malignant pleural effusion: development and validation of the LENT prognostic score

BACKGROUND: Malignant pleural effusion (MPE) causes debilitating breathlessness and predicting survival is challenging. This study aimed to obtain contemporary data on survival by underlying tumour type in patients with MPE, identify prognostic indicators of overall survival and develop and validate a prognostic scoring system. METHODS: Three large international cohorts of patients with MPE were used to calculate survival by cell type (univariable Cox model). The prognostic value of 14 predefined variables was evaluated in the most complete data set (multivariable Cox model). A clinical prognostic scoring system was then developed and validated. RESULTS: Based on the results of the international data and the multivariable survival analysis, the LENT prognostic score (pleural fluid lactate dehydrogenase, Eastern Cooperative Oncology Group (ECOG) performance score (PS), neutrophil-to-lymphocyte ratio and tumour type) was developed and subsequently validated using an independent data set. Risk stratifying patients into low-risk, moderate-risk and high-risk groups gave median (IQR) survivals of 319 days (228–549; n=43), 130 days (47–467; n=129) and 44 days (22–77; n=31), respectively. Only 65% (20/31) of patients with a high-risk LENT score survived 1 month from diagnosis and just 3% (1/31) survived 6 months. Analysis of the area under the receiver operating curve revealed the LENT score to be superior at predicting survival compared with ECOG PS at 1 month (0.77 vs 0.66, p<0.01), 3 months (0.84 vs 0.75, p<0.01) and 6 months (0.85 vs 0.76, p<0.01). CONCLUSIONS: The LENT scoring system is the first validated prognostic score in MPE, which predicts survival with significantly better accuracy than ECOG PS alone. This may aid clinical decision making in this diverse patient population

Search for Yukawa Production of a Light Neutral Higgs Boson at LEP

Within a Two-Higgs-Doublet Model (2HDM) a search for a light Higgs boson in the mass range of 4-12 GeV has been performed in the Yukawa process e+e- -> b bbar A/h -> b bbar tau+tau-, using the data collected by the OPAL detector at LEP between 1992 and 1995 in e+e- collisions at about 91 GeV centre-of-mass energy. A likelihood selection is applied to separate background and signal. The number of observed events is in good agreement with the expected background. Within a CP-conserving 2HDM type II model the cross-section for Yukawa production depends on xiAd = |tan beta| and xihd = |sin alpha/cos beta| for the production of the CP-odd A and the CP-even h, respectively, where tan beta is the ratio of the vacuum expectation values of the Higgs doublets and alpha is the mixing angle between the neutral CP-even Higgs bosons. From our data 95% C.L. upper limits are derived for xiAd within the range of 8.5 to 13.6 and for xihd between 8.2 to 13.7, depending on the mass of the Higgs boson, assuming a branching fraction into tau+tau- of 100%. An interpretation of the limits within a 2HDM type II model with Standard Model particle content is given. These results impose constraints on several models that have been proposed to explain the recent BNL measurement of the muon anomalous magnetic moment.Comment: 24 pages, 9 figures, Submitted to Euro. Phys. J.

Measurement of the Hadronic Cross-Section for the Scattering of Two Virtual Photons at LEP

The interaction of virtual photons is investigated using the reaction e+e- -> e+e- hadrons based on data taken by the OPAL experiment at e+e- centre-of-mass energies sqrt(s_ee)=189-209 GeV, for W>5 GeV and at an average Q^2 of 17.9 GeV^2. The measured cross-sections are compared to predictions of the Quark Parton Model (QPM), to the Leading Order QCD Monte Carlo model PHOJET to the NLO prediction for the reaction e+e- -> e+e-qqbar, and to BFKL calculations. PHOJET, NLO e+e- -> e+e-qqbar, and QPM describe the data reasonably well, whereas the cross-section predicted by a Leading Order BFKL calculation is too large.Comment: 30 pages, 10 figures, Submitted to Eur.Phys.J.