Toll-like receptor 2 expression on c-kit + cells tracks the emergence of embryonic definitive hematopoietic progenitors

International audienceHematopoiesis in mammalian embryos proceeds through three successive waves of hema-topoietic progenitors. Since their emergence spatially and temporally overlap and phenotypic markers are often shared, the specifics regarding their origin, development, lineage restriction and mutual relationships have not been fully determined. The identification of wave-specific markers would aid to resolve these uncertainties. Here, we show that toll-like receptors (TLRs) are expressed during early mouse embryogenesis. We provide phenotypic and functional evidence that the expression of TLR2 on E7.5 c-kit + cells marks the emergence of precursors of erythro-myeloid progenitors (EMPs) and provides resolution for separate tracking of EMPs from primitive progenitors. Using in vivo fate mapping, we show that at E8.5 the Tlr2 locus is already active in emerging EMPs and in progenitors of adult hematopoietic stem cells (HSC). Together, this data demonstrates that the activation of the Tlr2 locus tracks the earliest events in the process of EMP and HSC specification

Implementace environmentálního manažerského systému v podniku

This diploma thesis elaborates a proposal of an environmental management system implementation according to the ČSN EN ISO 14001:2016 standard in a selected company in Brno. Based on own activities in the company and in-depth interviews with the employees, the current situation and field of business of the company and its experience with the implementation of certifications were described. Furthermore, the company’s approach to environmental issues, including the definition of environmental aspects of the company, was identified. According to the individual chapters of the international standard, the thesis suggests steps to obtain the ISO certification. Furthermore, the thesis presents possible benefits and costs related to EMS implementation. The results of the interviews show whether the company has the necessary capacity to implement the standard and whether the certification is relevant for the company. Regardless of the suitability of the EMS for the company, appropriate measures to the environmental performance improvement are designed

Analýza nákladů pochodů sekundární metalurgie

Import 19/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Fakulta metalurgie a materiálového inženýrství. Katedra (634) ekonomiky a managementu v metalurgi

Studie produkce komunálního odpadu na území městské části Brno-sever

This thesis deals with communal waste generation within city district Brno-sever. The bachelor thesis is focused on description of communal waste generation and its components during time period 2012-2015. Overall, the components of communal waste management are set up for the whole city, so that the generation on the cadastral areas was derived from a total containers volume and frequency of transfer to a disposal facility. The results show the current situation of the communal waste generation and the proportion of its components in the relation to a character of the individual cadastral areas in the city district Brno-sever. In the comparison of the waste generation was found that the waste generation in the city district is by 10 kg per capita lower than in the whole city. Based on the analysis was determined the relationship between type of buildings or age of inhabitants and amount of generated waste. This dependence has not been proven. Even though, there has been another relationship identified, namely between waste generation and distance of the cadastral areas from the city centre. The same relationship also works for a proportion of its components in the cadastral areas of the city district Brno-sever

Charakteristika a uplatnění zásad petičního a stížnostního práva

Práce se zabývá zásadami petičního a stížnostního práva.Dokončená práce s úspěšnou obhajobo

Financování příspěvkových organizací na konkrétním případu - Speciální školy, Boleslavova 1, Praha 4

Diplomová práce je nejprve zaměřena na neziskové organizace a obecný vývoj neziskových aktivit v České republice. Dále je uvedena charakteristika příspěvkové organizace. Další část je věnována speciálnímu vzdělávání a speciálním školám. Praktická část diplomové práce se zabývá charakteristikou konkrétní příspěvkové organizace, a to Speciální školy, Boleslavova 1, Praha 4 a analýzou jejího hospodaření v letech 2001 - 2005

TCR triggering induces the formation of Lck-RACK1-actinin-1 multiprotein network affecting Lck redistribution

The initiation of T-cell signaling is critically dependent on the function of the member of Src family tyrosine kinases (SFKs), Lck. Upon TCR triggering, Lck kinase activity induces the nucleation of signal-transducing hubs that regulate the formation of complex signaling network and cytoskeletal rearrangement. In addition, the delivery of Lck function requires rapid and targeted membrane redistribution, but the mechanism underpinning this process is largely unknown. To gain insight into this process, we considered previously described proteins that could assist in this process via their capacity to interact with kinases and regulate their intracellular translocations. An adaptor protein, Receptor for Activated C Kinase 1 (RACK1), was chosen as a viable option and its capacity to bind Lck and aid the process of activation-induced redistribution of Lck was assessed. Our microscopic observation showed that T-cell activation induces a rapid, concomitant and transient co-redistribution of Lck and RACK1 into the forming immunological synapse. Consistent with this observation, the formation of transient RACK1-Lck complexes were detectable in primary CD4+ T-cells with their maximum levels peaking 10 seconds after TCR-CD4 co-aggregation. Moreover, RACK1 preferentially binds to a pool of kinase active pY394Lck which co-purifies with high molecular weight cellular fractions. The formation of RACK1-Lck complexes depends on functional SH2 and SH3 domains of Lck and includes several other signaling and cytoskeletal elements that transiently bind the complex. Notably, the F-actin-crosslinking protein, α-actinin-1, binds to RACK1 only in the presence of kinase active Lck suggesting that the formation of RACK1-pY394Lck-α-actinin-1 complex serves as a signal module coupling actin cytoskeleton bundling with productive TCR/CD4 triggering. In addition, the treatment of CD4+ T-cells with nocodazole, which disrupts the microtubular network, also blocked the formation of RACK1-Lck complexes. Importantly, activation-induced Lck redistribution was diminished in primary CD4+ T-cells by an adenoviral-mediated knock-down of RACK1. These results demonstrate that in T cells, RACK1 as an essential component of the multiprotein complex which upon TCR engagement links the binding of kinase active Lck to elements of the cytoskeletal network and affects the subcellular redistribution of Lck