Diagnóstico de Leishmaniosis canina mediante la técnica de reacción en cadena de la polimerasa (PCR) : un procedimiento simple para uso en la clínica

Para detectar la presencia de Leishmania ssp, en distintas muestras clínicas de perros sospechosos de padecer esta enfermedad, se ha utilizado la técnica PCR, amplificando para ello un fragmento del gen SSU rRNA, repetido más de 100 veces en el genoma del parásito. El método se ha optimizado para utilizarlo como método de rutina en la clínica. El procesado de las muestras es rápido y simple. El diagnóstico se ha realizado por presencia/ausencia del parásito, utilizando para ello muestras de sangre, médula ósea y ganglio linfático principalmente. En el caso de existir el parásito en el huésped se visualiza una banda nítida de un tamaño de 603 bp y en el caso de que el parásito esté ausente, no se detecta la presencia de esta banda. Los mejores resultados se obtuvieron cuando la muestra de partida fue médula o ganglio linfático. El método presenta la ventaja adicional de detectar portadores asintomáticos, incluidos los titulas de IFI dudosos. La técnica PCR se presenta como test de diagnóstico rutinario, siendo más rápida, eficaz y económica que los métodos de diagnóstico clásicos

First measurements of a scintillator based fast-ion loss detector near the ASDEX Upgrade divertor

A new reciprocating scintillator based fast-ion loss detector has been installed a few centimeters above the outer divertor of the ASDEX Upgrade tokamak and between two of its lower Edge Localized Modes (ELM) mitigation coils. The detector head containing the scintillator screen, Faraday cup, calibration lamp, and collimator systems are installed on a motorized reciprocating system that can adjust its position via remote control in between plasma discharges. Orbit simulations are used to optimize the detector geometry and velocity-space coverage. The scintillator image is transferred to the light acquisition systems outside of the vacuum via a lens relay (embedded in a 3D-printed titanium holder) and an in-vacuum image guide. A charge coupled device camera, for high velocity-space resolution, and an 8 × 8 channel avalanche photo diode camera, for high temporal resolution (up to 2 MHz), are used as light acquisition systems. Initial results showing velocity-space of neutral beam injection prompt losses and fast-ion losses induced by a (2, 1) neoclassical tearing mode are presented.EURATOM 63305

Atmospheric lepton fluxes at ultrahigh energies

In order to estimate the possibility to observe exotic physics in a neutrino telescope, it is essential to first understand the flux of atmospheric neutrinos, muons and dimuons. We study the production of these leptons by high-energy cosmic rays. We identify three main sources of muons of energy E > 10^6 GeV: the weak decay of charm and bottom mesons and the electromagnetic decay of unflavored mesons. Contrary to the standard assumption, we find that eta mesons, not the prompt decay of charm hadrons, are the dominant source of atmospheric muons at these energies. We show that, as a consequence, the ratio between the neutrino and muon fluxes is significantly reduced. For dimuons, which may be a background for long-lived staus produced near a neutrino telescope, we find that pairs of E ~ 10^7 GeV forming an angle above 10^-6 rad are produced through D (80%) or B (10%) meson decay and through Drell-Yan proceses (10%). The frequency of all these processes has been evaluated using the jet code PYTHIA.Comment: 10 pages, 4 figures; published versio

Diodos emisores de luz para irradiación de plantas

Los recientes desarrollos conseguidos en el área de la iluminación con diodos emisores de luz (LEDs, cuando son inorgánicos, y OLEDs, cuando son orgánicos) resultan de gran interés en horticultura, al permitir manipular el espectro radiante que va a incidir sobre las plantas, con el objetivo de aumentar su producción o de generar determinados efectos fisiológicos, sobre todo en invernaderos. Puesto que los vegetales crecen mejor cuando son iluminados (irradiados) en las regiones roja y azul del espectro, resulta aconsejable sustituir los sistemas de iluminación fotosintética actuales, fundamentalmente mediante lámparas de descarga en gases (vapor de sodio a alta presión y, en menor medida, halogenuros metálicos), por LEDs comerciales que emiten separadamente en esas regiones o por OLEDs susceptibles de emisión conjunta. Además, estos dispositivos son más eficientes que las lámparas incandescentes (consumen mucha menor energía eléctrica y poseen una vida media de uso mucho más larga), no generan exceso de calor (y por tanto no dañan a plantas térmicamente sensibles), e incluso, en el caso de los LEDs rojos, repelen insectos, por lo que contribuyen a la disminución en el uso de agrotóxicos. En el presente artículo, se revisan las últimas contribuciones en Fitofotónica relacionadas con los diodos emisores de luz, se aporta la experiencia existente sobre la aplicación de LEDs a invernaderos y se divulga el estado de las investigaciones que algunos grupos de investigación estamos realizando sobre OLEDs emisores bien en el rojo o bien en las regiones del rojo y el azul

A Comparison of Morphological, Jump, and Sprint Kinematic Asymmetries in Division I Track and Field Athletes

International Journal of Exercise Science 16(1): 1306-1319, 2023. Inter-limb asymmetries are the difference in performance in one limb with respect to the other. Running events in track and field are considered symmetrical while jumping and throwing events are considered asymmetrical. It is unknown if competing in these different events result in differences in inter-limb asymmetries, thus, this study compared the magnitude of jump, sprint, and morphological asymmetries in track and field athletes who compete in symmetrical and asymmetrical events. Forty-six Division I track and field athletes performed a series of vertical jumps (VJ) and broad jumps (BJ) with force platforms measuring peak force of each limb, and 30-meter fly sprints with kinematics (step length (SL), flight time (FT), and contact time (CT)) recorded during the sprints. Additionally, thirty-eight of these subjects underwent body composition analysis via dual x-ray absorptiometry to determine morphological asymmetries. Asymmetries were calculated using the symmetry index and the asymmetry measures were compared between sprinters, distance runners, throwers, and jumpers utilizing a one-way analysis of variance or Kruskal-Wallis tests with post-hoc comparisons as necessary. There were no differences in VJ, BJ, and sprint kinematic asymmetries found between groups but there were differences in leg fat mass asymmetries (H(3)=8.259, p=0.041, eta2= 0.101) as well as arm lean mass (H(3)=9.404, p=0.024, eta2=0.152), fat mass (H(3)=17.822,

Overview of progress in European medium sized tokamaks towards an integrated plasma-edge/wall solution

Integrating the plasma core performance with an edge and scrape-off layer (SOL) that leads to tolerable heat and particle loads on the wall is a major challenge. The new European medium size tokamak task force (EU-MST) coordinates research on ASDEX Upgrade (AUG), MAST and TCV. This multi-machine approach within EU-MST, covering a wide parameter range, is instrumental to progress in the field, as ITER and DEMO core/pedestal and SOL parameters are not achievable simultaneously in present day devices. A two prong approach is adopted. On the one hand, scenarios with tolerable transient heat and particle loads, including active edge localised mode (ELM) control are developed. On the other hand, divertor solutions including advanced magnetic configurations are studied. Considerable progress has been made on both approaches, in particular in the fields of: ELM control with resonant magnetic perturbations (RMP), small ELM regimes, detachment onset and control, as well as filamentary scrape-off-layer transport. For example full ELM suppression has now been achieved on AUG at low collisionality with n = 2 RMP maintaining good confinement HH(98,y2) 0.95. Advances have been made with respect to detachment onset and control. Studies in advanced divertor configurations (Snowflake, Super-X and X-point target divertor) shed new light on SOL physics. Cross field filamentary transport has been characterised in a wide parameter regime on AUG, MAST and TCV progressing the theoretical and experimental understanding crucial for predicting first wall loads in ITER and DEMO. Conditions in the SOL also play a crucial role for ELM stability and access to small ELM regimes.European Commission (EUROfusion 633053

High resolution time series reveals cohesive but short-lived communities in coastal plankton

© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nature Communications 9 (2018): 266, doi:10.1038/s41467-017-02571-4.Because microbial plankton in the ocean comprise diverse bacteria, algae, and protists that are subject to environmental forcing on multiple spatial and temporal scales, a fundamental open question is to what extent these organisms form ecologically cohesive communities. Here we show that although all taxa undergo large, near daily fluctuations in abundance, microbial plankton are organized into clearly defined communities whose turnover is rapid and sharp. We analyze a time series of 93 consecutive days of coastal plankton using a technique that allows inference of communities as modular units of interacting taxa by determining positive and negative correlations at different temporal frequencies. This approach shows both coordinated population expansions that demarcate community boundaries and high frequency of positive and negative associations among populations within communities. Our analysis thus highlights that the environmental variability of the coastal ocean is mirrored in sharp transitions of defined but ephemeral communities of organisms.This work was supported by grants from the U.S. National Science Foundation (OCE-1441943) to M.F.P. and the U.S. Department of Energy (DE-SC0008743) to M.F.P. and E.J.A. A.M.M.-P. was partially supported by the Ramon Areces foundation through a postdoctoral fellowship. D.J.M. was supported by the U.S. National Science Foundation (OCE-1314642) and National Institute of Environmental Health Sciences (1P01ES021923-01) through the Woods Hole Center for Oceans and Human Health

Implementação em Portugal de um estudo de prevalência da demência e da depressão geriátrica: a metodologia do 10/66 Dementia Research Group

ResumoIntroduçãoA demência e a depressão têm um impacto social muito significativo. Em Portugal, escasseiam dados epidemiológicos em pessoas idosas na comunidade. Em países em desenvolvimento, o 10/66 Dementia Research Group (DRG) conduziu múltiplos estudos sobre a prevalência e a incidência destes quadros. Os protocolos de avaliação 10/66‐DRG foram validados extensivamente nesses países, havendo vantagens potenciais na aplicação em regiões europeias com populações idosas de baixa escolaridade. Neste estudo, fundamentamos e descrevemos a implementação em Portugal da metodologia 10/66‐DRG para estudo da prevalência da demência e da depressão geriátrica.Materiais e métodosO trabalho de campo desenrola‐se numa fase única, em áreas geográficas definidas, sendo avaliados detalhadamente e de forma sistemática todos os residentes de 65 anos ou mais. A informação é recolhida dos participantes e de informadores próximos (www.alz.co.uk/1066/).ResultadosSelecionámos e mapeámos uma área urbana (em Fernão Ferro) e outra rural (em Mora), definindo amostras finais de 702 e 779 pessoas, respetivamente. Os instrumentos de avaliação foram cuidadosamente traduzidos e adaptados ao contexto português. Teve lugar um treino rigoroso dos entrevistadores, assegurando a fiabilidade de cotação interobservadores.Discussão e conclusãoA aplicação dos protocolos 10/66‐DRG permitirá obter dados robustos de prevalência de demência e de depressão, com amostras comunitárias de pessoas idosas em Portugal. Dada a validade internacional da metodologia utilizada, estes resultados serão comparáveis com os de outros centros 10/66, à escala mundial.AbstractIntroductionDementia and depression have a huge social impact. In Portugal, epidemiological data is scarce regarding community dwelling elderly people with these conditions. In developing countries, the 10/66 Dementia Research Group‐DRG has conducted multiple studies on the corresponding prevalence and incidence. The 10/66‐DRG protocols for population‐based studies were extensively validated in those countries, and there is a rationale to apply them in low literacy elderly populations in Europe. We describe the implementation of the protocols related to the prevalence study (on dementia and geriatric depression) in Portuguese settings.Materials and methodsThis is a one‐phase survey, in which all people 65+ years living in defined catchment areas are comprehensively evaluated. Information is collected directly from participants and from a related informant (www.alz.co.uk/1066/).ResultsWe selected and mapped an urban area (in Fernão Ferro) and a rural one (in Mora). The final samples were n=702 and n=779 respectively. Questionnaires were carefully translated and culturally adapted. Rigorous training procedures took place to ensure inter‐rater reliability.Discussion and conclusionsThe implementation of the 10/66‐DRG protocols will lead to robust data on the prevalence of dementia and depression in community samples of elderly people in Portugal. Given the international validity of our methodology, these results will be comparable with those from other 10/66 centres around the world