Tumor hypoxia and hypoxic markers in non-small cell lung cancer

Papers number 1, 2 and 4 of this thesis are not available in Munin due to publishers' restrictions: 1. Andersen S, Eilertsen M, Donnem T, Al Shibli K, Al-Saad S, Busund LT, Bremnes RM: 'Diverging prognostic impacts of hypoxic markers according to NSCLC histology', Lung Cancer (2011), 72, 294-302, available at http://dx.doi.org/10.1016/j.lungcan.2010.10.006 2. Eilertsen M, Pettersen I, Andersen S, Martinez I, Donnem T, Busund LT, Bremnes RM: 'In NSCLC, VEGF-A response to hypoxia may differ between squamous cell and adenocarcinoma histology', Anticancer Research (2012), 32, 4729-36. 4. Eilertsen M, Andersen S, Al-Saad S, Richardsen E, Stenvold H, Hald SM, Al-Shibli K, Donnem T, Busund LT, Bremnes RM: 'Positive prognostic impact of miR-210 in non-small cell lung cancer', Lung Cancer Volume 83, Issue 2, February 2014, Pages 272–278, available at http://dx.doi.org/10.1016/j.lungcan.2013.11.005</a

Sticky structures and opportunistic builders - The construction and social role of longhouses in northern Norway

The longhouse has been a turning point for research on prehistoric farming societies in Scandinavia for several decades. Yet, no comprehensive study has been made of this house type and its context, variations and social implications in the Far North. In this chapter, we present the currently available longhouse material within the three northernmost counties of Norway. The 50 longhouses that have been excavated within the administrative district of Tromsø University Museum are discussed in more detail. Our survey shows both similarities to and some intriguing variations from the longhouses in other areas of Norway and the rest of Scandinavia, concerning the chronology of various house types, building details and farm layout. All these aspects are obviously related to the particularities of the environmental, cultural and sociopolitical context in the north. However, the results are preliminary, as more thorough studies are needed, as well as more excavations employing current methodologies such as mechanical topsoil stripping, to ensure representative data

Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue

Prostate carcinoma is the most common cancer in men with few, quantifiable, biomarkers. Prostate cancer biomarker discovery has been hampered due to subjective analysis of protein expression in tissue sections. An unbiased, quantitative immunohistochemical approach provided here, for the diagnosis and stratification of prostate cancer could overcome this problem. Antibodies against four proteins BTF3, HINT1, NDRG1 and ODC1 were used in a prostate tissue array (> 500 individual tissue cores from 82 patients, 41 case pairs matched with one patient in each pair had biochemical recurrence). Protein expression, quantified in an unbiased manner using an automated analysis protocol in ImageJ software, was increased in malignant vs non-malignant prostate (by 2-2.5 fold, p<0.0001). Operating characteristics indicate sensitivity in the range of 0.68 to 0.74; combination of markers in a logistic regression model demonstrates further improvement in diagnostic power. Triple-labeled immunofluorescence (BTF3, HINT1 and NDRG1) in tissue array showed a significant (p<0.02) change in co-localization coefficients for BTF3 and NDRG1 co-expression in biochemical relapse vs non-relapse cancer epithelium. BTF3, HINT1, NDRG1 and ODC1 could be developed as epithelial specific biomarkers for tissue based diagnosis and stratification of prostate cancer

FP7-IDREEM project deliverable 5.1. Detailed plan and description of the future IMTA system at the Oldervika salmon farm (Nordland, Norway)

publishedVersio

Overpumping av makrell - Effekt på fangstkvalitet - Sammendrag av resultater fra tokt gjennomført i 2012, 2013 og 2014

Prosjektets hovedmål er å klarlegge hvorvidt det kan registreres forskjeller i makrellens kvalitet når den er pumpet direkte om bord i fangstfartøyet og når den er pumpet til et an·net fartøy (overpumping) etter at pumping til eget fartøy ble avsluttet. Denne rapporten gir en oppsummering av resultater fra 3 forsøk som ble gjennomført høsten 2012, 2013 og 2014, da med utgangspunkt i et ordinært notkast etter makrell for hvert av de respektive toktene. Resultatene viser at andel skader er høyere for fisk fangstet med ringnot sammenlignet med fisk fra kystnot. Dette skyldes sannsynligvis at pumpehastigheten er ca. 4 ganger raskere for ringnot sammenlignet med kystnot, i tillegg til større fangstmengder som også kan ha betydning. Videre viser resultatene at andelen fangstskader hos overpumpet fisk var høyere for ringnotfartøyet. Resultatene fra G-kraftloggerne viser at overpumping gir en økt påkjenning på fisken, samt et noe "berg- og dalbane" forløp for den overpumpet fisken.FiskeridirektoratetpublishedVersio

Fibroblast miR-210 overexpression is independently associated with clinical failure in Prostate Cancer – a multicenter (in situ hybridization) study

There is a need for better prognostication in prostate cancer (PC). “The micromanager of hypoxia”, microRNA-210 (miR-210) is directly linked to hypoxia, is overexpressed in PC and has been implied in tumor cell-fibroblast crosstalk. We investigated the prognostic impact of miR-210 in tumor cells and fibroblasts in PC. Tumor and stromal samples from a multicenter PC cohort of 535 prostatectomy patients were inserted into tissue microarrays. To investigate the expression of miR-210, we used in situ hybridization and two pathologists semiquantitatively scored its expression. Overexpression of miR-210 in tumor cells was not associated to biochemical failure-free survival (BFFS, p=0.85) or clinical failure-free survival (CFFS, p=0.09). However, overexpression of miR-210 in fibroblasts was significantly associated to a poor CFFS (p=0.001), but not BFFS (p=0.232). This feature was validated in both cohorts. Overexpression of miR-210 was independently associated with a reduced CFFS (HR=2.76, CI 95% 1.25–6.09, p=0.012). Overexpression of miR-210 in fibroblasts is independently associated with a poor CFFS. This highlights the importance of fibroblasts and cellular compartment crosstalk in PC. miR-210 is a candidate prognostic marker and potential therapeutic target in PC