Efectos del contenido en fibra, almidón y proteína del pienso de peridestete sobre los gazapos y las conejas

Este trabajo se planteó como un diseño factorial para estudiar, en un sistema de alimentación conjunta, los efectos de i) la sustitución de almidón por FAD, ii) la sustitución de almidón por fibra digestible y iii) la reducción del contenido de PB en el pienso de peridestete, sobre la producción tanto de conejas como de gazapos.Martínez Vallespín, B. (2008). Efectos del contenido en fibra, almidón y proteína del pienso de peridestete sobre los gazapos y las conejas. http://hdl.handle.net/10251/13409Archivo delegad

Porcine Colostrum Protects the IPEC-J2 Cells and Piglet Colon Epithelium against Clostridioides (syn. Clostridium) difficile Toxin-Induced Effects

Clostridioides difficile toxins are one of the main causative agents for the clinical symptoms observed during C. difficile infection in piglets. Porcine milk has been shown to strengthen the epithelial barrier function in the piglet’s intestine and may have the potential to neutralise clostridial toxins. We hypothesised that porcine colostrum exerts protective effects against those toxins in the IPEC-J2 cells and in the colon epithelium of healthy piglets. The IPEC-J2 cells were treated with either the toxins or porcine colostrum or their combination. Analyses included measurement of trans-epithelial electrical resistance (TEER), cell viability using propidium iodide by flow cytometry, gene expression of tight junction (TJ) proteins and immune markers, immunofluorescence (IF) histology of the cytoskeleton and a TJ protein assessment. Colon tissue explants from one- and two-week-old suckling piglets and from five-week-old weaned piglets were treated with C. difficile toxins in Ussing chamber assays to assess the permeability to macromolecules (FITC-dextran, HRP), followed by analysis of gene expression of TJ proteins and immune markers. Toxins decreased viability and integrity of IPEC-J2 cells in a time-dependent manner. Porcine colostrum exerted a protective effect against toxins as indicated by TEER and IF in IPEC-J2 cells. Toxins tended to increase paracellular permeability to macromolecules in colon tissues of two-week-old piglets and downregulated gene expression of occludin in colon tissues of five-week-old piglets (p = 0.05). Porcine milk including colostrum, besides other maternal factors, may be one of the important determinants of early immune programming towards protection from C. difficile infections in the offspring

El cine como recurso educativo en las aulas de Historia y sus dificultades

El trabajo se inicia con una pequeña introducción acerca del estado actual de la sociedad y de la educación en referencia a los recursos audiovisuales tanto en el día a día como al uso de los mismo en las aulas. Posteriormente, se tratan los problemas principales que plantea la introducción del cine, documentales y otros documentos audiovisuales en el aula como un recurso didáctico a través del cual enseñar historia a los alumnos yendo desde la consabida falta de tiempo, común a cualquier tipo de innovación didáctica, como a la falta de formación en el lenguaje audiovisual de profesores y alumnos.<br /

Effects of feeding programme on the performance and energy balance of nulliparous rabbit does

A total of 190 rabbit females were used to evaluate five feeding programmes from 9 weeks of age to the first parturition: CAL, fed ad libitum with a control diet (C: 11.0 MJ digestible energy (DE) and 114 g digestible protein (DP)/kg dry matter (DM)) until first parturition; CR, fed ad libitum with C diet until 12 weeks of age and then C diet restricted (140 g/day) until first parturition; F, fed ad libitum with a low-energy, high-fibre diet (F: 8.7 MJ DE and 88 g DP/kg DM) until first parturition; FC, fed with F diet ad libitum until 16 weeks of age, and C diet ad libitum until first parturition; FCF, fed with F diet ad libitum until 16 weeks of age, then C diet ad libitum until 20 weeks and then F diet ad libitum until first parturition. The rabbits were artificially inseminated at 18 weeks of age. CAL group had a higher mortality rate compared with the other groups between 9 and 12 weeks of age (34% v. 3%; P,0.05) and during the last 3 weeks of first pregnancy (14% v. 3%; P,0.05). The CAL and FC females presented higher BW and perirenal fat thickness (PFT) than CR females at 11 days of pregnancy (10.41 kg and 10.6 mm; P,0.05), with F females showing medium values. The type of feeding procedure did not affect the fertility rate of young females at first artificial insemination. Differences in BW disappeared at parturition, when only CAL females presented a greater PFT than CR and FC females (10.3mm; P,0.05). In comparison with FCF, CAL females had smaller and thinner live born litters (22.5 kits and 2139 g, respectively; P,0.05), with CR, F and FC females showing medium values. The low number of kits born alive for CAL females was because of their lesser total number of kits born (21.7 kits; P,0.05) and the greater mortality of their litters at birth (113.9%; P,0.05) compared with FCF females. Non-esterified fatty acid was higher in the blood of females fed C diet (CAL and CR) than in others at partum day (on average 10.15 mmol/l; P,0.05). In conclusion, the ad libitum use of diets for lactating rabbit does throughout the rearing period could lead young rabbit females to present a higher risk of early death and smaller litter size at first parturition. Feed restriction or earlier use of suitably fibrous diets led females to achieve the critical BW and fat mass at first mating to ensure reproduction.The authors thank the Spanish Ministry of Education and Science (Project AGL2006-07596) for the economic support to conduct this study.Martínez Paredes, EM.; Ródenas Martínez, L.; Martínez Vallespín, B.; Cervera Fras, MC.; Blas Ferrer, E.; Brecchia, G.; Boiti, C.... (2012). Effects of feeding programme on the performance and energy balance of nulliparous rabbit does. Animal. 6(7):1086-1095. https://doi.org/10.1017/S1751731111002643S1086109567Pascual, J. J., Castella, F., Cervera, C., Blas, E., & Fernández-Carmona, J. (2000). The use of ultrasound measurement of perirenal fat thickness to estimate changes in body condition of young female rabbits. Animal Science, 70(3), 435-442. doi:10.1017/s135772980005178xArias-Álvarez, M., García-García, R. M., Rebollar, P. G., Nicodemus, N., Revuelta, L., Millán, P., & Lorenzo, P. L. (2009). Effects of a lignin-rich fibre diet on productive, reproductive and endocrine parameters in nulliparous rabbit does. Livestock Science, 123(2-3), 107-115. doi:10.1016/j.livsci.2008.10.013Sejrsen, K., Huber, J. T., Tucker, H. A., & Akers, R. M. (1982). Influence of Nutrition on Mammary Development in Pre- and Postpubertal Heifers. Journal of Dairy Science, 65(5), 793-800. doi:10.3168/jds.s0022-0302(82)82268-6Klindt, J., Yen, J. T., & Christenson, R. K. (1999). Effect of prepubertal feeding regimen on reproductive development of gilts. Journal of Animal Science, 77(8), 1968. doi:10.2527/1999.7781968xBlas, C. de, & Mateos, G. G. (s. f.). Feed formulation. Nutrition of the rabbit, 222-232. doi:10.1079/9781845936693.0222Rommers, J. M., Kemp, B., Meijerhof, R., & Noordhuizen, J. P. (2001). The effect of litter size before weaning on subsequent body development, feed intake, and reproductive performance of young rabbit does. Journal of Animal Science, 79(8), 1973. doi:10.2527/2001.7981973xRommers, J. M., Meijerhof, R., Noordhuizen, J. P. T. M., & Kemp, B. (2002). Relationships between body weight at first mating and subsequent body development, feed intake, and reproductive performance of rabbit does. Journal of Animal Science, 80(8), 2036-2042. doi:10.1093/ansci/80.8.2036Moschos, S., Chan, J. L., & Mantzoros, C. S. (2002). Leptin and reproduction: a review. Fertility and Sterility, 77(3), 433-444. doi:10.1016/s0015-0282(01)03010-2Xiccato, G., Bernardini, M., Castellini, C., Dalle Zotte, A., Queaque, P. I., & Trocino, A. (1999). Effect of postweaning feeding on the performance and energy balance of female rabbits at different physiological states. Journal of Animal Science, 77(2), 416. doi:10.2527/1999.772416xChilliard, Y., Ferlay, A., Faulconnier, Y., Bonnet, M., Rouel, J., & Bocquier, F. (2000). Adipose tissue metabolism and its role in adaptations to undernutrition in ruminants. Proceedings of the Nutrition Society, 59(1), 127-134. doi:10.1017/s002966510000015xNicodemus, N., García, J., Carabaño, R., & De Blas, J. C. (2007). Effect of substitution of a soybean hull and grape seed meal mixture for traditional fiber sources on digestion and performance of growing rabbits and lactating does1. Journal of Animal Science, 85(1), 181-187. doi:10.2527/jas.2005-365Bezille P 1995. Toxémie de gestation et hypocalcémie chez la brebis. In Maladies métaboliques des rumiants (ed. Jean Marc Bichet), pp. 101–104. Le Point Vétérinarie 27(special number), WK Publications, Paris, France.Blas, E., & Gidenne, T. (s. f.). Digestion of sugars and starch. Nutrition of the rabbit, 19-38. doi:10.1079/9781845936693.0019FRISCH, R. E. (1984). BODY FAT, PUBERTY AND FERTILITY. Biological Reviews, 59(2), 161-188. doi:10.1111/j.1469-185x.1984.tb00406.xGidenne, T. (1997). Caeco-colic digestion in the growing rabbit: impact of nutritional factors and related disturbances. Livestock Production Science, 51(1-3), 73-88. doi:10.1016/s0301-6226(97)00111-5Klindt, J., Yen, J. T., & Christenson, R. K. (2001). Effect of prepubertal feeding regimen on reproductive development and performance of gilts through the first pregnancy. Journal of Animal Science, 79(4), 787. doi:10.2527/2001.794787xKlindt, J., Yen, J. T., & Christenson, R. K. (2001). Level of dietary energy during prepubertal growth and reproductive development of gilts. Journal of Animal Science, 79(10), 2513. doi:10.2527/2001.79102513xLittell, R. C., Henry, P. R., & Ammerman, C. B. (1998). Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science, 76(4), 1216. doi:10.2527/1998.7641216xRebollar, P. G., Pereda, N., Schwarz, B. F., Millán, P., Lorenzo, P. L., & Nicodemus, N. (2011). Effect of feed restriction or feeding high-fibre diet during the rearing period on body composition, serum parameters and productive performance of rabbit does. Animal Feed Science and Technology, 163(1), 67-76. doi:10.1016/j.anifeedsci.2010.10.005Rommers, J. M., Meijerhof, R., Noordhuizen, J. P. T. M., & Kemp, B. (2004). Effect of feeding program during rearing and age at first insemination on performances during subsequent reproduction in young rabbit does. Reproduction Nutrition Development, 44(4), 321-332. doi:10.1051/rnd:2004037Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74(10), 3583-3597. doi:10.3168/jds.s0022-0302(91)78551-2Whitehead, C. C. (1988). Selection for leanness in broilers using plasma lipoprotein concentration as selection criterion. Leanness in Domestic Birds, 41-57. doi:10.1016/b978-0-408-01036-8.50007-5Sørensen, M. T., Danielsen, V., & Busk, H. (1998). Different rearing intensities of gilts: Livestock Production Science, 54(2), 159-165. doi:10.1016/s0301-6226(97)00176-0Xiccato, G., & Trocino, A. (s. f.). Energy and protein metabolism and requirements. Nutrition of the rabbit, 83-118. doi:10.1079/9781845936693.0083Brecchia, G., Bonanno, A., Galeati, G., Federici, C., Maranesi, M., Gobbetti, A., … Boiti, C. (2006). Hormonal and metabolic adaptation to fasting: Effects on the hypothalamic–pituitary–ovarian axis and reproductive performance of rabbit does. Domestic Animal Endocrinology, 31(2), 105-122. doi:10.1016/j.domaniend.2005.09.006European Union 2003. Protection of animals used for experimental purposes. Directive 86/609/EEC of 24th November 1986, amended 16th September 2003.Martínez-Vallespín, B., Martínez-Paredes, E., Ródenas, L., Cervera, C., Pascual, J. J., & Blas, E. (2011). Combined feeding of rabbit female and young: Partial replacement of starch with acid detergent fibre or/and neutral detergent soluble fibre at two protein levels. Livestock Science, 141(2-3), 155-165. doi:10.1016/j.livsci.2011.05.014Marteniuk, J. V., & Herdt, T. H. (1988). Pregnancy Toxemia and Ketosis of Ewes and Does. Veterinary Clinics of North America: Food Animal Practice, 4(2), 307-315. doi:10.1016/s0749-0720(15)31050-1Barb, C. ., Barrett, J. ., Kraeling, R. ., & Rampacek, G. . (2001). Serum leptin concentrations, luteinizing hormone and growth hormone secretion during feed and metabolic fuel restriction in the prepuberal gilt. Domestic Animal Endocrinology, 20(1), 47-63. doi:10.1016/s0739-7240(00)00088-

Fiber Composition in Sows’ Diets Modifies Clostridioides difficile Colonization in Their Offspring

Dietary fiber has a potential to modulate the gut microbiota in sows. We hypothesized that a maternal diet rich in either high- or low-fermentable fiber during gestation and lactation influences Clostridioides difficile gut colonization in suckling piglets. Twenty sows were fed gestation and lactation diets enriched with either high-fermentable sugar beet pulp (SBP) or low-fermentable lignocellulose (LNC) fibers. C. difficile, toxin B (TcdB), fecal score, microbial abundance (16S-rDNA sequencing) and metabolites were measured in the feces from the sows and their piglets. C. difficile concentration was higher in piglets from the sows fed LNC than SBP along the study (P ≤ 0.05). Higher prevalence of C. difficile was noted in three-week-old piglets from sows fed LNC vs. SBP (45% vs. 0%, P = 0.001). TcdB prevalence was higher in six-day-old piglets from the sows fed LNC vs. SBP (60% vs. 17%, P = 0.009). In sows, fecal microbial metabolites were higher in SBP than LNC, while C. difficile concentration showed no difference. Higher microbial diversity Shannon index was noted in sows from SBP vs. LNC one week before parturition and at the parturition (P ≤ 0.05). Piglets from SBP vs. LNC tended to have higher microbial diversity Shannon index at two and three weeks of age. Diets enriched with high-fermentable fiber compared to low-fermentable fiber in sows reduced C. difficile colonization in their piglets. Susceptibility to colonization by C. difficile in neonatal piglets can be modulated by the sows’ diet, supporting the hypothesis of the early microbial programming in the offspring and the importance of the sow-piglet couple

Feeding programmes based on highly-digestible fibre weaning diets: effects on health, growth performance and carcass and meat quality in rabbits

The effect of three different dietary programmes on health, growth performance and carcass and meat quality in young rabbits weaned at 28 d was studied using a diet (F) rich in highly-digestible fibre, from 17 to 63 d of age (group FF); using diet F from 17 to 42 d followed by a diet poor in highly-digestible fibre and rich in starch and fat (S) until 63 d (group FS); and using a standard diet C with intermediate highly-digestible fibre and starch content, containing 100 ppm of zinc-bacitracin, from 17 to 63 d (group CC). A highly-digestible fibre diet could be useful to reduce the incidence of digestive disorders. However, it decreased slaughter weight (2294 g in FF vs. 2406 g in CC; P<0.05) and carcass and meat traits, e.g. dressing out percentage (55.4% in FF vs. 56.7% in CC; P<0.05), meat to bone ratio (5.73 in FF vs. 5.94 in CC; P<0.05) and hind leg fat content (3.81% in FF vs. 4.71% in CC; P<0.05) at 63 d of age. Switching to a high starch and fat diet at late fattening improved chilled carcass weight at 63 d of age (1339 g in FS vs. 1263 g in FF; P<0.05) mainly through the promotion of liver development (7.53% in group FS vs. 6.47% in group FF; P<0.05) and fat deposition (3.89% in FS vs. 2.63% in FF; P<0.05), and increased hind leg fat content (+1.2 points of fat percentage; P<0.05). However, this switch increased health risk (35.1% in FS vs. 17.6% in FF; P<0.05).This study was supported by the Interministerial Commission for Science and Technology (CICYT) from the Spanish Government Grant number AGL2011-30170-C02-01 is gratefully acknowledged. Funding from the Spanish Ministry of Economy and Competitiveness for Madam Pascual's contract (PTA2011-5888-T) is also gratefully acknowledged.Pascual Amorós, MDLD.; Soler Sanchis, MD.; Cervera Fras, MC.; Pla Torres, M.; Pascual Amorós, JJ.; Blas Ferrer, E. (2014). Feeding programmes based on highly-digestible fibre weaning diets: effects on health, growth performance and carcass and meat quality in rabbits. Livestock Science. 169:88-95. https://doi.org/10.1016/j.livsci.2014.07.007S889516

Clostridioides difficile-mesocolonic oedema in neonatal suckling piglets develops regardless of the fibre composition in sow’s diets

Maternal dietary factors have been reported to influence Clostridioides difficile colonisation in the offspring. Twenty suckling piglets from sows fed diets supplemented with high-fermentable sugar beet pulp (SBP) or low-fermentable lignocellulose (LNC) fibres during gestation and lactation were dissected in the first week after birth. Postmortem analysis included clinical mesocolon and faecal scoring, concentration of C. difficile and respective toxins in colon digesta and faeces, immunoglobulins in serum and inflammatory markers in serum and colon tissues. Sow colostrum was assessed for nutrients, immunoglobulins and biogenic amines. Toxin-neutralising IgG antibodies were measured in colostrum and serum of the sows, and in colon digesta and serum of the piglets. Mesocolonic oedema of different severity was present in most of the piglets from both sows’ feeding groups. Concentrations of C. difficile, toxins and calprotectin in colon digesta and faecal contents did not differ between the study piglets. Calprotectin correlated positively with mesocolon score (rho = 413, P = 0.07). Piglets from sows fed LNC vs SBP tended to have higher IgA (P = 0.089), IgG (P = 0.053), total Ig (P = 0.053), albumin (P = 0.075) and total protein content (P = 0.007) in serum. Colon tissues of piglets from the SFB vs LNC had upregulated expression of ZO-1 (P = 0.021), PCNA (P = 0.015) and TGF-β (P = 0.014). Titers of anti-toxin-IgG-antibodies in serum and colostrum and in piglet colon digesta and serum did not differ between sows from both dietary groups, but they all showed strong positive correlations. In conclusion, dietary sugar beet pulp or lignocellulose fed to sows did not influence the concentrations of C. difficile and toxins titers in colon digesta and faeces of neonatal piglets

Partial replacement of starch with acid detergent fibre and/or neutral detergent soluble fibre at two protein levels: Effects on ileal apparent digestibility and caecal environment of growing rabbits

[EN] The effects of the composition of pen-weaning diets on apparent ileal digestibility of nutrients and caecal environment were studied in growing rabbits. Eight diets were formulated according to a 2 x 2 x 2 factorial design to increase acid detergent fibre (ADF) from 230 to 290 g kg(-1) dry matter (DM) at the expense of mainly starch, to increase neutral detergent soluble fibre (NDSF) from 145 to 185 g kg(-1) DM at the expense of mainly starch, and to reduce crude protein (CP) content from 175 to 145 g kg(-1) DM. A total of 32 litters of eight kits were randomly assigned to the diets at 17 days of age, weaned at 28 days of age and slaughtered at 35 days of age to collect samples of ileal and caecal contents. The substitution of ADF for starch reduced the coefficient of apparent ileal digestibility (CAID) of DM (0.292 vs. 0.229; P <0.01). The substitution of NDSF for starch increased the CAID of NDSF (-0.040 vs. 0.099; P < 0.01). An interaction between dietary ADF and NDSF levels was found for the CAID of CP, higher in the low ADF-low NDSF diets than in the rest (+0.074; P <0.05). The reduction of dietary CP content decreased the CAID of CP (0.578 vs. 0.525; P < 0.05) and NDSF (0.086 vs. -0.038; P <0.01). Interactions between dietary ADF and NDSF levels were found affecting full caecum weight as well as DM content and pH of caecal digesta. The increase of ADF in the high NDSF diets reduced full caecum weight (-9.1 g kg(-1) live weight; P < 0.01), increased DM content of caecal digesta (+31 g kg(-1); P <0.001) and reduced caecal pH (- 0.10; P < 0.05). In the low NDSF diets, higher ADF increased DM content of caecal digesta to a lesser extent (+16 g kg(-1); P < 0.001) and increased caecal pH (+0.08; P <0.05). The increase of NDSF in the high ADF diets increased full caecum weight (+8.5 g kg(-1) live weight; P <0.01) and reduced caecal pH (-0.14; P <0.001). In the low ADF diets, higher NDSF increased full caecum weight more extensively (+15.5 g kg(-1) live weight; P < 0.001) and reduced DM content of caecal digesta (-16 g kg(-1); P <0.001). Both ADF and NDSF replacing starch increased total volatile fatty acid (VFA) concentration of caecal digesta (+11.5% and +13.9%, respectively; P < 0.01). The replacement with NDSF increased molar proportion of butyrate (0.095 vs. 0.112; P <0.001) at the expense of mainly acetate (0.841 vs. 0.820; P <0.001) and reduced ammonia concentration of caecal digesta (-8.1%; P < 0.05). The reduction of dietary CP content decreased total VFA (-14.4%; P < 0.001) and ammonia (-23.5%; P < 0.001) concentrations of caecal digesta and increased caecal pH (5.87 vs. 5.93; P <0.05). Overall, the three dietary changes under study led to changes in the caecal environment which might be effective in reducing mortality rate in a context of rabbit epizootic enteropathy. (C) 2013 Elsevier B.V. All rights reserved.Financial support was provided by the Ministerio de Educacion y Ciencia of Spain (Proyecto AGL2006-07596). Authors thank J. Garcia from the Universidad Politecnica de Madrid (Spain) for the analyses of ytterbium.Martínez Vallespín, B.; Martinez-Paredes, E.; Ródenas Martínez, L.; Moya, V.; Cervera Fras, MC.; Pascual Amorós, JJ.; Blas Ferrer, E. (2013). Partial replacement of starch with acid detergent fibre and/or neutral detergent soluble fibre at two protein levels: Effects on ileal apparent digestibility and caecal environment of growing rabbits. Livestock Science. 154(1-3):123-130. https://doi.org/10.1016/j.livsci.2013.02.012S1231301541-

Dietary fiber and its role in performance, welfare, and health of pigs

Dietary fiber (DF) is receiving increasing attention, and its importance in pig nutrition is now acknowledged. Although DF for pigs was frowned upon for a long time because of reductions in energy intake and digestibility of other nutrients, it has become clear that feeding DF to pigs can affect their well-being and health. This review aims to summarize the state of knowledge of studies on DF in pigs, with an emphasis on the underlying mode of action, by considering research using DF in sows as well as suckling and weaned piglets, and fattening pigs. These studies indicate that DF can benefit the digestive tracts and the health of pigs, if certain conditions or restrictions are considered, such as concentration in the feed and fermentability. Besides the chemical composition and the impact on energy and nutrient digestibility, it is also necessary to evaluate the possible physical and physiologic effects on intestinal function and intestinal microbiota, to better understand the relation of DF to animal health and welfare. Future research should be designed to provide a better mechanistic understanding of the physiologic effects of DF in pigs

Combined feeding of rabbit female and young: Partial replacement of starch with acid detergent fibre or/and neutral detergent soluble fibre at two protein levels

To evaluate the effects of the composition of weaning diets used for combined feeding both on the performance and body condition of rabbit does and on the growth and health of young rabbits, eight experimental diets were formulated according to a factorial design 2×2×2, the three factors being: i) partial replacement of starch with acid detergent fibre (ADF), ii) partial replacement of starch with neutral detergent soluble fibre (NDSF) and iii) reduction of the crude protein (CP) content from 175 to 145g kg-1 dry matter (DM). The study involved 138 rabbit does and 318 litters fed experimental diets from 17th to 28th day of lactation (weaning). Thereafter, 2371 weaned rabbits (784 in individual cages and 1587 in collective ones) continued receiving the same experimental diets until 49days of age and a commercial finishing diet until 60days of age. Replacing starch with ADF increased feed intake of does (+26±5g DM day-1, P<0.001) but, only with high CP diets, impaired their milk yield (-15±6g day-1, P<0.01) and perirenal fat thickness (PFT) change (-0.41±0.13mm, P<0.01), as well as litter weight at weaning (-154±52g, P<0.01); from weaning to 49days of age, this replacement increased feed intake (+14±2g DM day-1, P<0.001) and impaired live weight gain (-1.6±0.7g day-1, P<0.05), but reduced mortality rate (-10.2%, P<0.001). Replacing starch with NDSF reduced feed intake of does, in greater extent with high CP diets (-34±6g DM day-1, P<0.001) than with low CP diets (-19±7g DM day-1, P<0.01), as well as their milk yield (-16±4g day-1, P<0.001), with impairing PFT change only with high CP diets (-0.30±0.13mm, P<0.05); litter feed intake and weight at weaning were also negatively affected (-14.4±2.5g DM day-1, -202±38g, P<0.001); from weaning to 49days of age, this replacement reduced feed intake (-8±2g DM day-1, P<0.001), impaired live weight gain only with low CP diets (-2.3±1.0g day-1, P<0.05) and reduced mortality rate (-15.1%, P<0.001). Reducing CP content affected negatively feed intake of does (-22±5g DM day-1, P<0.001), as well as their milk yield, this impairment being more important with more starch-less ADF diets (-24±5g day-1, P<0.001) than with less starch-more ADF diets (-13±6g day-1, P<0.05), and, only with more starch-less ADF diets, PFT change (-0.36±0.13mm, P<0.01); litter feed intake was affected negatively (-20.1±2.4g DM day-1, P<0.001), as well as litter weight at weaning, in great extent when fed more starch-less ADF diets (-581±52g, P<0.001) than when fed less starch-more ADF diets (-403±54g, P<0.001); from weaning to 49days of age, this dietary change reduced feed intake (-6±2g DM day-1, P<0.001) and impaired live weight gain (-5.0±0.7g, P<0.001), but reduced mortality rate (-19.9%, P<0.001). Overall, the three dietary changes under study impaired milk yield, body condition of does and litter weight at weaning, although the negative effect of replacing starch with ADF was only observed in the high CP diets; moreover, growth during post-weaning period also decreased. However, in an Epizootic Rabbit Enteropathy context, mortality rate during this period was reduced by these three dietary changes, which effects seemed additive. © 2011 Elsevier B.V.Financial support was provided by the Spanish Ministerio de Educacion y Ciencia (Proyecto AGL2006-07596).Martínez Vallespín, B.; Martinez-Paredes, E.; Ródenas Martínez, L.; Cervera Fras, MC.; Pascual Amorós, JJ.; Blas Ferrer, E. (2011). Combined feeding of rabbit female and young: Partial replacement of starch with acid detergent fibre or/and neutral detergent soluble fibre at two protein levels. Livestock Science. 141(2-3):155-165. https://doi.org/10.1016/j.livsci.2011.05.014S1551651412-