How are gene sequences analyses modifying bacterial taxonomy? The case of Klebsiella

Bacterial names are continually being changed in order to more adequately describe natural groups (the units of microbial diversity) and their relationships. The problems in Klebsiella taxonomy are illustrative and common to other bacterial genera. Like other bacteria, Klebsiella spp. were isolated long ago, when methods to identify and classify bacteria were limited. However, recently developed molecular approaches have led to taxonomical revisions in several cases or to sound proposals of novel species. [Int Microbiol 2004; 7(4):261-268

El codesarrollo en España. Protagonistas, discursos y experiencia

Depto. de Antropología Social y Psicología SocialFac. de Ciencias Políticas y SociologíaFALSEpu

Financial literacy and risk-taking propensity as predictors of undergraduates' entrepreneurial intention

Entrepreneurial intention, as one of the best entrepreneurship predictors, is related to the individual's choice to start a new business. Entrepreneurship education is, without a doubt, the topic that arouses the greatest interest in the entrepreneurship research community. However, empirical evidence is contradictory regarding the influence of entrepreneurship education on entrepreneurial intention, leading academics to the recent claim to delve into the existing heterogeneity regarding the relationship between knowledge, skills and competencies and entrepreneurial intention. Consequently, this article proposes individuals' financial literacy as a relevant predictor of undergraduates' entrepreneurial intention. Financial literacy-entrepreneurship evidence is scarce and limited: (1) they do not focus on entrepreneurial intention; (2) they incompletely measure the multidimensional construct of financial literacy; (3) they do not analyse undergraduates, despite being an important starting point for entrepreneurship. Therefore, this article applies covariance-based structural equation models to 568 Spanish undergraduates with business and tourism degrees to obtain the impact of financial literacy (measured through its three dimensions, i.e., financial knowledge, attitude, and behaviour) on entrepreneurial intention through the mediating role of risk-taking propensity. The results show that: (1) financial knowledge positively influences indirectly; (2) financial attitude negatively influences indirectly; (3) financial behaviour positively influences both directly and indirectly. These findings are highly relevant for the entrepreneurship research community in general and policymakers in particular since we demonstrate the entrepreneurial potential derived from undergraduates' financial literacy thanks to the mediating role of risk-taking propensity, which establishes a novel channel to promote entrepreneurship and, thus, solve the serious youth unemployment problems.Ministerio de Educación y Formación Profesional. Formación de Profesorado Universitario (FPU20/02328). Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Exploring the relationship between financial literacy, risk-taking propensity and entrepreneurship in Spanish undergraduates

This article uses covariance-based structural equation models in a sample of 568 Spanish undergraduates to provide evidence on the impact of financial literacy (measured through its three dimensions, that is, financial knowledge, attitude, and behaviour) on entrepreneurial intention through the mediating role of the risk-taking propensity. The results show that: (i) financial knowledge has an indirect positive impact; (ii) financial attitude has an indirect negative impact; (iii) financial behaviour has a positive impact both directly and indirectly. These findings are highly relevant in demonstrating the mechanism that explains the relationship between financial literacy and entrepreneurial intention considering the role of risk-taking propensity, an important channel to promote youth entrepreneurship.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Impacto en la mortalidad por el uso de amikacina inhalatoria en pacientes con neumonía grave / Impact of the use of inhaled amikacin on mortality in patients with severe Pneumonia

Introducción: En la actualidad, la resistencia antimicrobiana es un elemento importante en las unidades de cuidados intensivos.Objetivo: Evaluar el impacto del uso de los aminoglucósidos inhalatorios en la mortalidad de pacientes con neumonía grave.Métodos: Estudio analítico longitudinal retrospectivo en el periodo diciembre 2021 - marzo 2023. Universo: 84 pacientes, con diagnóstico de neumonía grave, mediante cultivo de secreciones respiratorias, positivo a Klebsiella pneumoniae, Pseudomona o Enterobacter al ingreso en UCI. Muestreo probabilístico error alfa 1,96, confiabilidad de 0,05. Todos recibieron iguales pautas de tratamiento. Se dividieron en tres grupos Grupo 1-Amikacina inhalatoria y Cefalosporinas; Grupo 2: amikacina, cefalosporinas 3 era y ciprofluoxacino; Grupo 3: Cefalosporinas 3 era y ciprofluoxacino.Resultados: Al ingreso, distribución similar entre grupos de APP p= 0,87. Sexo p=0,09. Distribución de cultivos negativos a los 5 días Klebsiella (grupo 2 -77 %) (Grupo 1 - 60 %) (Grupo 3-27 %) P=0,06. Enterobacter (grupo 2 - 83 %) p = 0,302. Mortalidad global en el orden de 32 %. Sobrevida al egreso (grupo 1 - 13 pacientes con 72 %). Grupo 2 - 15 (83 %). Grupo 3- 8 (7 %) P=0,06. Luego de reagrupar los pacientes según el uso del fármaco inhalatorio mortalidad grupo 1-2 donde se utilizó el fármaco inhalatorio sobrevida 28 pacientes (78 %). P=0,02. OR 0,25 IC 0,74-0,87.Conclusiones: El uso de los fármacos aminoglucósidos es una estrategia viable en la terapéutica de los pacientes graves y críticos con Neumonía. Reduce la mortalidad de pacientes con gérmenes dentro del espectro de actividad. No se evidenció elementos de toxicidad local ni sistémica

Acquisition activity: do firm age and family control matter?

This article examines the relationship between firm age and acquisition activity and how family and non-family firms differ in the number of acquisitions they undertake. Inspired by previous research requiring firm age as a focal aspect and literature studying the antecedents of acquisitions, we draw on the SEW perspective to test our hypotheses based on the analysis of the acquisition activity of Asia-Pacific public firms. Our empirical findings support a U-shaped relationship between firm age and acquisition activity. Moreover, the findings reveal that family firms engage in fewer acquisitions than non-family firms irrespective of the age of the firm.Funding for open Access charge: Universidad de Málaga / CBU

La sensibilización musical como fuente de aprendizaje en un mundo poco sonoro

This research purpose was the design of a pedagogical proposal that contributes to the musical awareness of the hearing impaired population of the Institución Educativa Normal Superior in Neiva city, as a way to contribute in the didactics of inclusive music education, applying a qualitative research methodological approach, with an exploratory, descriptive and participatory design, using documentary analysis, participant observation and interview as instruments for data collection. The sample of the study population considered 12 deaf children, out of 48 in total that exists in the afternoon session, with diversity in their hearing impairment, with whom a series of musical perception workshops were conducted, exposing them to the vibrations and qualities of music. It was found that the deaf population under study, has a great affinity with music, but little approach, because they have not been allowed to access it, much less include it in their curriculum, violating the right to attend music class with their hearing peers. In general, it is concluded that in art there are no barriers and that, undoubtedly, there are no better or worse ways of perceiving music, but simply different and equally valid for everyone, generating a search for actions that allow the acceptance of the deaf person as an individual, who, because of their linguistic difference, needs additional and specialized support.La presente investigación tuvo como finalidad el diseño de una propuesta pedagógica que contribuya a la sensibilización musical de la población con limitación auditiva de la Institución Educativa Normal Superior de la ciudad de Neiva, como un aporte a la didáctica de la educación musical inclusiva, aplicando un enfoque metodológico de investigación cualitativo, con un diseño exploratorio, descriptivo y participativo, utilizando como instrumentos de recolección de información el análisis documental, la observación participante y la entrevista. La muestra de la población en estudio, estuvo constituida por 12 niños y niñas Sordos, de 48 en total que existen en la jornada de la tarde, con diversidad en su discapacidad auditiva, con los cuales se realizó una serie de talleres de percepción musical, exponiéndolos a las vibraciones y cualidades de la música. se encontró que la población sorda en estudio, tiene una gran afinidad con la música, pero poco se acercan, debido a que no se les ha brindado la oportunidad de acceder a ella y mucho menos incluirla dentro de su currículo, vulnerado el derecho de asistir a clase de música con sus compañeros oyentes. En general se concluye que en el arte no hay barreras y que, sin duda, no existen formas mejores o peores de percibir la música, sino simplemente diferentes e igualmente válidas para todos, generando una búsqueda de acciones que permita la aceptación de la persona Sorda como un individuo, que, por su diferencia lingüística, necesita de apoyos adicionales y especializados

El método Delphi y la formación del profesorado en TIC

Este método se ha utilizado para dar respuesta a uno de los objetivos de un proyecto investigación subvencionado por el Ministerio de Ciencia e Innovación que pretende diseñar, producir y evaluar un entorno de aprendizaje 2.0 para profesores universitarios. En esta comunicación se expone la utilización del método Delphi para la selección de los contenidos a incluir en un “Entorno Personal de Aprendizaje” cuya finalidad es la capacitación del profesorado universitario en TIC. En concreto, el método Delphi, se ha utilizado para dar respuesta a cuáles con las temáticas básicas que hay que trabajar para capacitar al profesor universitario en el manejo didáctico de las TIC. De la aplicación de este método, enriquecido con las aportaciones del numeroso grupo de expertos (68), se ofrece una lista de tres bloques (Aspectos introductorios, Herramientas Servicio y Recursos para la formación en contextos de Educación Superior, Aspectos Metodológicos y Evaluación) con sus diferentes líneas temáticas y con ejemplos-sugerencias de contenidos para cada una de ellas

Analysis of the bacterial diversity associated with the roots of maize ( Zea mays L.) through culture-dependent and culture-independent methods

The present study investigated bacterial diversity associated with the roots of maize through the use of culture-dependent and culture-independent methods. Bacterial 16S–23S rDNA internal transcribed spacer sequences (ITS) primers were used to amplify sequences obtained directly from the root matrix by Percoll gradient separation. This assay showed that ã-Proteobacteria within Enterobacter, Erwinia, Klebsiella, Pseudomonas, and Stenotrophomonas genera were predominant groups. The culturable component of the bacterial community was also assessed, revealing that the predominant group was Firmicutes, mainly of Bacillus genus, while Achromobacter, Lysinibacillus, and Paenibacillus genera were rarely found in association with the roots. Only two genera within ã-Proteobacteria, Enterobacter and Pseudomonas, were found in the culture collection. Differences in richness and diversity between the rhizospheric and endophytic bacterial communities were also evidenced. The spectrum of bacteria naturally associated with maize roots is wide and the magnitude of such diversity will depend on the methods chosen for analysis. The knowledge of this spectrum will facilitate the search of microorganisms capable of exerting antagonism to diverse pathogens or detecting plant growth enhancersFil: Pereira, Paola Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Ibañez, Fernando Julio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Rosenblueth Laguette, Mónica Teresa. Universidad Nacional Autónoma de México; MéxicoFil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martínez Romero, Esperanza. Universidad Nacional Autónoma de México; Méxic

The Symbiome of Llaveia Cochineals (Hemiptera: Coccoidea: Monophlebidae) Includes a Gammaproteobacterial Cosymbiont Sodalis TME1 and the Known Candidatus Walczuchella monophlebidarum

The genome and transcriptome of the endosymbiotic flavobacterium Candidatus Walczuchella monophlebidarum revealed its role in the synthesis of essential amino acids for its host, the wax cochineal Llaveia axin axin. There were, however, missing genes in the endosymbiont for some biosynthetic pathways. Here, we characterized TME1, another cochineal symbiont that may metabolically complement Walczuchella. TME1 was ascribed to the gammaproteobacterial genus Sodalis on a phylogenomic basis using gene sequences from 143 proteins core genome sequences and the core average nucleotide identity (ANI) confirmed its position. Additionally, we describe Sodalis as a coherent genus. TME1 genome is around 3.4 Mb and has complete gene sequences for the biosynthesis of 10 essential amino acids, for polyamines, flagella, nitrate respiration, and detoxification among many others. Transcripts from ovaries and bacteriomes allowed the identification of differentially transcribed genes from the endosymbionts and host. Highly transcribed genes were identified in TME1 and transcripts involved in amino acid biosynthesis were found. We review here that cosymbionts that derived from different bacterial classes and genera seem to be advantageous for insects that have Flavobacteria as the primary endosymbionts