Manual de producción del sistema acuaponía del Centro Agroempresarial y Acuícola

El presente, es un manual de producción del sistema acuapónico de la unidad productiva acuícola del centro Agroempresarial y acuícola de la Regional Guajira, realizada como instrumento, que recopila la documentación de procedimientos que conforman las actividades ejecutadas en el sistema acuaponia del centro de formación, además, sirve como guía para aprendices, instructores, productores y población en general, sobre el desarrollo de la producción acuícola bajo el enfoque acuapónico.Generalidades -- Antecedentes -- Acuicultura -- Hidroponía --. Acuaponía -- Descripción del sistema acuaponía de la unidad productiva acuícola del CAA -- Procedimiento para la producción de especies animales “Peces” -- Mantenimiento, limpieza y desinfección de los tanques de producción -- Siembra de Peces -- Nutrición y suministro de alimento -- Cosecha, sacrificio y comercialización de la producción -- Procedimiento para la producción de especies vegetales -- Preparación de las camas hidropónicas y sistema NFT -- Instalación y condiciones de invernadero -- Siembra y mantenimiento de las semillas -- Trasplante de especies vegetales al sistema hidropónico -- Mantenimiento de especies vegetales -- Análisis del desarrollo vegetal y recolección de frutos -- Parámetros de operación del sistema acuapónico -- Controles aplicados en el sistema acuaponía -- Medición de parámetros del agua -- Aplicación de sal marina -- Melaza como medida de control -- Resultados -- Producción de especies animales -- Producción de especies vegetalesna49 página

Uma nova abordagem para entender a escolha modal e de rotas dos pedestres em Portugal

O presente trabalho visa analisar os fatores que interferem nas escolhas que as pessoas fazem de modos de transporte ou caminhos a pé, tendo em conta quatro grupos de variáveis: geométricos, sintáticos, uso do solo e acessibilidade de transportes. Para isso, foi formulado um modelo de escolha discreta em duas partes: escolha do modo e escolha de caminhos (a pé). Os resultados mostraram que fatores como segurança, conforto e forma urbana contribuem significativamente para a escolha dos caminhos. Para a escolha do modo, a maioria dos fatores determinantes foram: tempo e períodos do dia, sendo que ambos são exemplos de fatores que não são controláveis; e elementos econômicos e o tempo de deslocamento. Para a escolha da bicicleta, os principais fatores foram a presença de ciclovias e bicicletários. Foi possível concluir que o carro é o modo de transporte com maior preferência natural, seguida de caminhada e finalmente bicicleta

Oral and dental health status in patients with eating disorders in Madrid, Spain

The aim of the present study was to describe and compare the oral and dental health status of two groups, one diagnosed with eating disorders (EDs), and another group without this pathology, assessing the following oral manifestations: dental alterations, periodontal disorders, soft tissue disorders, non-stimulated salivary flow, and oral pH. This comparative transversal epidemiological study included 179 participants, of whom 59 were diagnosed with EDs (Eating Disorder Group: EDG) and 120 had no antecedents of EDs (No Eating Disorder Group: NEDG). All patients fulfilled the following inclusion criteria: women aged over 18 years, diagnosed with an ED by a specialist, patients who had undergone at least 1 year monitoring by the Clinical Nutrition Unit, and had not received any periodontal treatment during the previous 6 months. Both groups were homogeneous in terms of sex, age, education, and socioeconomic level. Oral exploration was performed, registering clinical variables, as well as sociodemographic and socioeconomic data, oral hygiene habits, and smoking. Statistical significance was established as p 95%). The dental erosion (DE) was the most significative feature of dental alterations. The degree of DE was significantly greater in the EDG (p<0.001). A significant association between soft tissue lesions and EDs was found (p<0.001) A notable difference in non-stimulated salivary flow was found between the groups (p<0.001). No significant differences between the groups were found for periodontal status, dental caries, or oral hygiene practices. On the basis of the results obtained, it is necessary to carry out oral/dental examination as soon as an ED is diagnosed with regular check-ups thereafter

Effect of plant extracts on the inhibition of the mycelial growth of Penicilium citrinum Link

Objective: Evaluate in vitro plant extracts of three plant species: mistletoe (Psittacanthus spp.), skunk grass (Petiveria alliacea) and ginger (Zingiber officinale), to determine the inhibitory effect on mycelial growth of Penicilliun citrinum isolated from coffee beans. Design/methodology/approach: Filtrations were performed under aseptic conditions using a vacuum system. These were added to the Potato Dextrose Agar medium, once solidified a 5 mm disc of P. citrinum was placed in the center of the Petri dish. Results: The ethyl plant extracts showed inhibition of (100%) on the development of the pathogen as well as the chemical product. Findings/conclusions: Ethyl plant extracts can be an agroecological alternative to control P. citrinum.Objective: To evaluate in vitro plant extracts of three plant species —mistletoe (Psittacanthus spp.), guinea hen weed (Petiveria alliacea), and ginger (Zingiber officinale)— with the aim of determining their inhibitory effect on the mycelial growth of Penicilliun citrinum isolated from coffee beans.Design/Methodology/Approach: Filtrations were carried out under aseptic conditions using a vacuum system and were added to the Potato Dextrose Agar medium. Once it had solidified, a 5-mm disc of P. citrinum was placed in the center of the Petri dish.Results: The ethyl plant extracts, like the chemical product, showed a 100% inhibition on the pathogen development.Findings/Conclusions: Ethyl plant extracts can be an agroecological alternative for the control of P. citrinum

Tumor P70S6K hyperactivation is inversely associated with tumor-infiltrating lymphocytes in triple-negative breast cancer

Purpose: Triple-negative breast cancer (TNBC) is characterized by large heterogeneity and relative lack of available targeted therapies. To find therapeutic strategies for distinct patients with TNBC, several approaches have been used for TNBC clustering, including recently immune and phosphoproteomic patterns. Based on 70-kDa ribosomal protein S6 kinase (P70S6K)-TNBC clustering, the current study explores the immune profiling in TNBC tumors. Methods: Stromal tumor-infiltrating lymphocytes (sTILs) were evaluated in human TNBC tumor samples. Furthermore, immunohistochemistry staining for CD8, CD4, Foxp3, and CD20 was performed in tissue microarrays (TMA) sections. Results: Histological analysis showed decreased sTILs, CD20+ cells, and CD8+/CD4+ ratio in high phosphorylated P70S6K (p-P70S6K) tumors. Moreover, p-P70S6K score was directly correlated with CD4+ and Foxp3+ T cells, while it was inversely correlated with CD8+/CD4+ and CD8+/Foxp3+ ratios. Conclusion: sTIL infiltration and lymphocyte profiling vary in the context of hyperactivation of P70S6K in TNBC tumorsThe project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 893597. RC is a recipient of the ISCIII grants: PI17/01865 and PI20/01458. MQF is a recipient of the following Grants: AES-PI19/00454 funded by the ISCIII and co-funded by the European Regional Development Fund (ERDF) and B2017/BMD3733 (Immunothercan-CM)—Call for Coordinated Research Groups from Madrid Region—Madrid Regional Government—ERDF funds. The study was also funded by CRIS Contra el Cancer Foundatio

Impacto do desenho da malha viária na mobilidade urbana

The paper analyzes the performance of different street plans to determine which variables are present in each type, as first step to creating a walkability index. Three neighborhoods of Lisbon are used as case studies: (a) Campo de Ourique - with a grid street plan - (b) Graça ”“ with irregular roads - and (c) Telheiras - with a contemporary street plan. It was found that the grid-based street plan has more road homogeneity, a lower straightness index and a higher average integration index. The contemporary street plan performs the worst, while the organic street plan is ranked in the middle.&nbsp;O artigo explora o desempenho de diferentes malhas viárias de modo a apresentar quais variáveis estão mais presentes em cada tipo de malha criando diretrizes para a composição de um índice de caminhabilidade. São utilizados três bairros de Lisboa como estudos de caso: (a) Campo de Ourique ”“ cuja malha é regular ”“ (b) Graça ”“ caracterizado pela irregularidade ”“ e (c) Telheiras ”“ cujo desenho apresenta características contemporâneas. Verificou-se que a malha regular apresenta maior homogeneidade viária, menor índice de conversão e maior média de integração, enquanto a malha contemporânea apresentou o pior desempenho e a malha orgânica, por sua vez, uma posição intermediária

Small extracellular vesicle targeting of hypothalamic AMPKα1 promotes weight loss in leptin receptor deficient mice

Background and aims: Leptin receptor (LEPR) deficiency promotes severe obesity and metabolic disorders. However, the current therapeutic options against this syndrome are scarce. Methods: db/db mice and their wildtypes were systemically treated with neuronal-targeted small extracellular vesicles (sEVs) harboring a plasmid encoding a dominant negative mutant of AMP-activated protein kinase alpha 1 (AMPKα1-DN) driven by steroidogenic factor 1 (SF1) promoter; this approach allowed to modulate AMPK activity, specifically in SF1 cells of the ventromedial nucleus of the hypothalamus (VMH). Animals were metabolically phenotyped. Results: db/db mice intravenously injected with SF1-AMPKα1-DN loaded sEVs showed a marked feeding-independent weight loss and decreased adiposity, associated with increased sympathetic tone, brown adipose tissue (BAT) thermogenesis and browning of white adipose tissue (WAT).Conclusion: Overall, this evidence indicates that specific modulation of hypothalamic AMPK using a sEV-based technology may be a suitable strategy against genetic forms of obesity, such as LEPR deficiencyMinisterio de Ciencia y Universidades co-funded by the FEDER Program of EU (CD: BFU2017-87721; RN: RTI2018-099413-B-I00 and RED2018-102379-T; ML: RTI2018-101840-B-I00, PID2021-128145NB-I00 and PDC2022-133958-I00). “la Caixa” Foundation (ID100010434), under the agreement LCF/PR/HR19/52160022 (ML); EuroNanoMed III (RA & ML: EURONANOMED2019-050-ENAMEP); European Research Council (RN: ERC Synergy Grant-2019-WATCH-810331)S