The Concept of “Sala de Fabrica”: On-Site Museums to Raise Awareness of Cultural Heritage After a Restoration Project

A conservation process usually generates new knowledge and an enormous amount of documentation during the inception and implementation of the project: the information collected from archives and other institutions; the information provided by the preliminary studies carried out prior to the intervention; the data provided in the field during the works and at the end of the process; and the final set of documentation delivered to the institution responsible for the maintenance and management of the monument. The challenge for conservation professionals and cultural heritage managers throughout this process once the works are over is to achieve and transmit this information to the public and specialists in order to raise awareness for better conservation of our built heritage. During the last few years, one of the actions that the Caja Madrid Foundation has activated with its restoration projects has been the opening of permanent on site museums or “Salas de Fábrica”, a place on site to understand the restoration works, to exhibit the remains that have being retrieved during the project and to permit the public to better understand the historical and artistic values of architectural and archaeological heritage as well as the importance of preserving our cultural legacy for the future

The Concept of “Sala de Fabrica”: On-Site Museums to Raise Awareness of Cultural Heritage After a Restoration Project

A conservation process usually generates new knowledge and an enormous amount of documentation during the inception and implementation of the project: the information collected from archives and other institutions; the information provided by the preliminary studies carried out prior to the intervention; the data provided in the field during the works and at the end of the process; and the final set of documentation delivered to the institution responsible for the maintenance and management of the monument. The challenge for conservation professionals and cultural heritage managers throughout this process once the works are over is to achieve and transmit this information to the public and specialists in order to raise awareness for better conservation of our built heritage. During the last few years, one of the actions that the Caja Madrid Foundation has activated with its restoration projects has been the opening of permanent on site museums or “Salas de Fábrica”, a place on site to understand the restoration works, to exhibit the remains that have being retrieved during the project and to permit the public to better understand the historical and artistic values of architectural and archaeological heritage as well as the importance of preserving our cultural legacy for the future

DNA expansions generated by human Polμ on iterative sequences

Polm is the only DNA polymerase equipped with template-directed and terminal transferase activities. Polm is also able to accept distortions in both primer and template strands, resulting in misinsertions and extension of realigned mismatched primer terminus. In this study, we propose a model for human Polm-mediated dinucleotide expansion as a function of the sequence context. In this model, Polm requires an initial dislocation, that must be subsequently stabilized, to generate large sequence expansions at different 50-P-containing DNA substrates, including those that mimic non-homologous endjoining (NHEJ) intermediates. Our mechanistic studies point at human Polm residues His329 and Arg387 as responsible for regulating nucleotide expansions occurring during DNA repair transactions, either promoting or blocking, respectively, iterative polymerization. This is reminiscent of the role of both residues in the mechanism of terminal transferase activity. The iterative synthesis performed by Polm at various contexts may lead to frameshift mutations producing DNA damage and instability, which may end in different human disorders, including cancer or congenital abnormalitiesMinisterio de Ciencia y Tecnologia [BFU2009-10085 and CONSOLIDER CSD2007-00015]; Fundacion Ramon Areces (to Centro de Biologia Molecular ‘‘Severo Ochoa’’); Ministerio de Educacion y Ciencia (to A.A.); Comunidad Autonoma de Madrid (to M.J.M.). Funding for open access charge: Comunidad de Madrid grant [S2011/BMD-2361

Abnormal magnetic behaviour of powder metallurgy austenitic stainless steels sintered in nitrogen

The magnetic response of AISI 304L and AISI 316L obtained through powder metallurgy and sintered in nitrogen were studied. AISI 304L sintered in nitrogen showed a ferromagnetic behaviour in as-sintered state while AISI 316L was paramagnetic. After solution annealing both were paramagnetic. Magnetic behaviour was analysed by using a vibrating sample magnetometer, a magnetic ferritscope and magnetic etching. A microstructural characterization was performed by means of optical metallography, X-ray diffraction, scanning electron microscopy (SEM) and energy dispersive analysis of X-rays (EDS). Some samples when needed were submitted to aged heat treatments at 675 and 875 °C for 90 min, 4, 6, 8 or 48 h. The main microstructural feature found was the presence of a lamellar constituent formed by nitride precipitates and an interlamellar matrix of austenite and/or ferrite. The abnormal magnetic response was explained based on this.Dirección General de Programas y Transferencia de Conocimiento, Ministerio de Ciencia e Innovación, España, MAT2008-0278

La Millora de la formació inicial de mestres: el Programa de Millora i Innovació en la Formació de Mestres, MIF

Aquest article exposa les accions i els resultats assolits en els dos anys de funcionament del Programa de Millora i Innovació en la Formació de Mestres, impulsat pel Consell Interuniversitari de Catalunya. S'hi expliquen i s'hi valoren algunes de les mesures adoptades per millorar-ne el model, s'hi exposen altres actuacions liderades pel Programa i s'hi anuncien alguns dels reptes de futur per a la formació inicial de mestres.This paper presents the action taken and the results achieved in the Improvement and Innovation in Initial Teacher Education Program (MIF Program) since its creation two years ago. It briefly explains and evaluates some of the measures adopted to improve the system, presenting other actions spearheaded by the Program and announcing some challenges for the future in initial teacher education

Nucleotide sequence and transfer properties of two novel types of Actinobacillus pleuropneumoniae plasmids carrying the tetracycline resistance gene tet(H)

17 p.The aim of this study is to analyze the sequence and transfer properties of two tetracycline resistance plasmids found in clinical isolates of Actinobacillus pleuropneumoniae in order to assert their role in the spread of tetracycline resistenceS

Antimicrobial susceptibility patterns of Haemophilus parasuis from pigs in the United Kingdom and Spain

18 p.A total of 30 British and 30 Spanish Haemophilus parasuis isolates were tested for their susceptibility to 19 of the antimicrobials currently used in swine practice with a broth microdilution method in order to know the emergence of resistance against these compounds in this porcine pathogen. All the British isolates were susceptible to penicillin, ceftiofur, erythromycin, tilmicosin, enrofloxacin, and florfenicol, and most of them were susceptible to the remaining antimicrobials (the highest resistance rate found was of 20% to neomycin). In contrast, all the Spanish isolates were susceptible exclusively to florfenicol, and high proportions of resistance were encountered for penicillin, ampicillin, oxytetracycline, erythromycin, tilmicosin, tiamulin and trimethoprim + sulphamethoxazole; in addition, a bimodal or multimodal distribution, or tailing of Spanish isolates over the MIC range was observed for clindamycin, sulphonamides and tylosine tartrate, suggesting the development of acquired resistance. In addition, several multiresistance patterns were found among the Spanish isolates, 23.3% of them being resistant to at least eight antimicrobials, the same rate as that encountered for those being susceptible to all antimicrobials tested. This study showed that in general British H. parasuis isolates are susceptible to antimicrobial agents routinely used for treatment of porcine respiratory diseases; however, the Spanish isolates need a more continuous surveillance of their susceptibility patternsS

Efectos de calcimax® sobre la nutrición cálcica del manzano tipo Golden y su influencia sobre el bitter pit

4 Pags.- 2 Tabls.El bitter pit es un desorden fisiológico que aparece generalmente durante el almacenamiento del fruto. La aplicación de tratamientos foliares con calcio durante la estación es el método más utilizado para disminuir la incidencia de esta fisiopatía ya que su aparición se relaciona con una deficiencia de este nutriente en el fruto. Calcimax® es una nueva formulación con calcio para la prevención y control del bitter pit. En este trabajo se describen dos de los experimentos realizados para evaluar el Calcimax® como corrector de bitter en las condiciones de cultivo del Valle del Ebro. Los tratamientos realizados con Calcimax®, siguiendo distintas estrategias de aplicación, sobre dos variedades de manzano Tipo Golden incrementaron el contenido de calcio en fruto, en mayor medida, con aplicaciones tardías. La concentración de Calcimax® al 0,5 % mostró una ligera reducción o retraso en el nivel de afección de bitter pit, aunque tras varios meses de almacenamiento, su incidencia fue muy elevada en todos los casos.Trabajo realizado bajo el proyecto AGL2001-2260.Peer reviewe

Effect of vanadium carbide on dry sliding wear behavior of powder metallurgy AISI M2 high speed steel processed by concentrated solar energy

Mixtures of AISI M2 high speed steel and vanadium carbide (3, 6 or 10 wt.%) were prepared by powder metallurgy and sintered by concentrated solar energy (CSE). Two different powerful solar furnaces were employed to sinter the parts and the results were compared with those obtained by conventional powder metallurgy using a tubular electric furnace. CSE allowed significant reduction of processing times and high heating rates. The wear resistance of compacts was studied by using rotating pin-on-disk and linearly reciprocating ball-on-flat methods. Wear mechanisms were investigated by means of scanning electron microscopy (SEM) observations and chemical inspections of the microstructures of the samples. Better wear properties than those obtained by conventional powder metallurgy were achieved. The refinement of the microstructure and the formation of carbonitrides were the reasons for this.JCCM in the Spanish National Programme (Project PPIC10-0052-5968

Identification by proximity labeling of novel lipidic and proteinaceous potential partners of the dopamine transporter

Dopamine (DA) transporters (DATs) are regulated by trafficking and modulatory processes that probably rely on stable and transient interactions with neighboring proteins and lipids. Using proximity-dependent biotin identification (BioID), we found novel potential partners for DAT, including several membrane proteins, such as the transmembrane chaperone 4F2hc, the proteolipid M6a and a potential membrane receptor for progesterone (PGRMC2). We also detected two cytoplasmic proteins: a component of the Cullin1-dependent ubiquitination machinery termed F-box/LRR-repeat protein 2 (FBXL2), and the enzyme inositol 5-phosphatase 2 (SHIP2). Immunoprecipitation (IP) and immunofluorescence studies confirmed either a physical association or a close spatial proximity between these proteins and DAT. M6a, SHIP2 and the Cullin1 system were shown to increase DAT activity in coexpression experiments, suggesting a functional role for their association. Deeper analysis revealed that M6a, which is enriched in neuronal protrusions (filopodia or dendritic spines), colocalized with DAT in these structures. In addition, the product of SHIP2 enzymatic activity (phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2]) was tightly associated with DAT, as shown by co-IP and by colocalization of mCherry-DAT with a specific biosensor for this phospholipid. PI(3,4)P2 strongly stimulated transport activity in electrophysiological recordings, and conversely, inhibition of SHIP2 reduced DA uptake in several experimental systems including striatal synaptosomes and the dopaminergic cell line SH-SY5Y. In summary, here we report several potential new partners for DAT and a novel regulatory lipid, which may represent new pharmacological targets for DAT, a pivotal protein in dopaminergic function of the brainOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by grants from the Spanish MINECO (RTI2018-098712-B-100) and the “Fundación Ramón Areces”, the latter also providing an institutional grant to CBMS