Wintertime connections between extreme wind patterns in Spain and large-scale geopotential height field

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Probabilistic and deterministic results of the ANPAF analog model for Spanish wind field estimations

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Value of Indirect Hemagglutination and Coagglutination Tests for Serotyping Haemophilus parasuis

P. 880-882An indirect hemagglutination test (IHA) and a coagglutination test (CA) were evaluated using saline, boiled, and autoclaved extracts for serotyping Haemophilus parasuis. CA showed several cross-reactions, whereas IHA gave rise to specific reactions, with minor exceptions. IHA was further compared with the immunodiffusion test (the “gold standard”) for the serotyping of 67 field isolates. As a conclusion, IHA is recommended as a useful method for sensitive and specific serotyping of H. parasuisS

Variation in the synthesis of microcystin in response to saline and osmotic stress in Microcystis ruginosa PCC7806

The growth and synthesis of microcystin in Microcystis aeruginosa PCC7806 was studied in cells grown in the presence of NaCl or with sucrose. The saline stress caused a decrease in the transcript levels of mcyD, one of the genes involved in microcystin synthesis, which was correlated with a decrease in the content of microcystin-LR in the cells. The cells treated with sucrose also had reduced levels of mcyD transcripts and contents of microcystin-LR

Investigación cualitativa en mujeres víctimas de violencia de género

ObjetivoAnalizar la experiencia de mujeres víctimas de la violencia de género atendidas en centros de atención primaria desde el punto de vista de las mujeres.DiseñoInvestigación cualitativa interpretativa. Perspectiva fenomenológica.EmplazamientoEstudio multicéntrico en centros de salud urbanos.ParticipantesMujeres víctimas de la violencia de género (física, psíquica, sexual)atendidas en los centros de salud. Muestreo intencional y teórico hasta el punto de saturación. Criterios de segmentación: edad (jóvenes-edad media-ancianas); maltrato actual o pasado; detección en urgencies-consultas.Mediciones principalesRelatos biográficos y análisis de contenido de la transcripción literal de las grabaciones. Codificación mediante programa NUD-IST. Utilización para la interpretación de la Teoría Fundamentada.LimitacionesComplejidad del fenómeno de estudio. Proyección de la perspectiva del investigador. Volumen ingente de datos. Se proponen estrategias para mejorar la credibilidad, la conformabilidad y la transferibilidad.Aplicabilidad prácticaMejorar el conocimiento de la situación de las mujeres víctimas de la violencia doméstica desde una perspectiva no directiva, lo que permitirá mejorar la calidad de las intervenciones.ObjectiveTo analyse, from the point of view of the women, the experience of women who are victims of male violence and attended at primary care centres.DesignInterpretative, qualitative research. Phenomenological perspective.SettingMulti-centre study in urban health centres.ParticipantsWomen victims of male violence (physical, psychological, or sexual) seen at health centres. Intention andtheoretical sampling to saturation point.Segmentation criteria: age (young/middle-aged/elderly); current or past ill-treatment; detection in casualty/consultations.Main measurementsBiographical accounts and content analysis of recordings’ literal transcription. Coding through the NUDIST programme. Use for interpreting Well-Founded Theory.LimitationsComplexity of the phenomenon under study. Projection of researcher’s perspective. Huge amount of data. Strategies are proposed for increasing credibility, conformity and transferability.Practical useTo improve understanding of the situation of women who are victims of domestic violence, from a non-directive perspective that enables the quality of interventions to be improved

Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor

P. 49-59Bacteria have evolved a set of highly specialized proteins to capture iron in irondepleted environments. The acquisition and uptake of iron present in the extracellular milieu of eukaryotic organisms is indispensable for the growth and survival of microbial pathogens in the course of infection. Haemophilus parasuis is the causative agent of Glässer disease, which is responsible for considerable financial losses in pig-rearing worldwide. To gain insight into the mechanisms involved in siderophore-mediated iron uptake in H. parasuis, genes in the H. parasuis ferric hydroxamate uptake (Fhu) region were amplified in the work being reported here. As has been described in A. pleuropneumoniae, an Fhu genomic region was also present in H. parasuis, being composed of four potential consecutive open reading frames (ORF) designated as fhuC, fhuD, fhuB, and fhuA, respectively. By immunoblotting, using a cross-reactive polyclonal antibody raised against Actinobacillus pleuropneumoniae FhuA protein, it was demonstrated that this protein was constitutively expressed in H. parasuis and its level of expression was not modified under conditions of restricted iron availability. This is the first report describing the presence of the fhu genes in H. parasuis. Our results indicate that FhuA protein expression is not affected under ironrestricted conditions, however, it is one of the targets of the humoral immune responseS

In Vivo IS6110 profile changes in a Mycobacterium tuberculosis strain as determined by tracking over 14 years

Transposition and homologous recombination of IS6110 appear in Mycobacterium tuberculosis along in vivo sequential infec- tions. These events were checked in different clones of a successful strain, M. tuberculosis Zaragoza, with the focus on a variant in which integration of a copy of IS6110 in the origin of replication (oriC) region occurred

Highly water-stable rare ternary Ag-Au-Se nanocomposites as long blood term X-rays computed tomography contrast agents

X-ray computed tomography (CT) is a powerful and widely used medical non-invasive technique that requires intravenously administration of contrast agents to enhance the sensitivity and visualization of soft tissues. In this work, we have developed a novel CT contrast agent based on ternary Ag-Au-Se chalcogenide nanoparticles. A facile and gentle ligand exchange by using a 3 kDa PEGylated ligand with a dithiol dihydrolipoic as an anchor resulted in highly water-soluble and monodisperse nanoparticles. Moreover, the injected PEGylated ternary NPs presented excellent characteristics as a CT contrast agent with high bioavailability, low cytotoxicity and long blood circulation times with slow uptake by the mononuclear phagocyte system, thus being ideal for in vivo imaging

Bioadhesión sobre Alúmina. Propiedades superficiales

La alúmina es uno de los materiales cerámicos de mayor relevancia en usos biomédicos. Así, este trabajo hace una completa caracterización de su superficie a través de propiedades que son de especial importancia en aquellos procesos de interacción con superficies biológicas como son células y microorganismos. Tensión superficial, potencial zeta y rugosidad superficial se analizan utilizando métodos diversos. La tensión superficial se muestra en torno a 35 mJ m-2 aunque los valores dependen en muchos casos de la aproximación escogida y de los grupos de líquidos prueba necesarios para su determinación. El punto isoeléctrico aparece a pH = 4,6 lo que indica una carga superficial negativa cuando la alúmina se encuentre inmersa en líquidos fisiológicos (pH = 7). Los accidentes topográficos responsables de la macrorugosidad pueden considerarse de periodicidad en torno a 25um presentando alta variabilidad a escalas inferiores, por ello, es importante determinar la rugosidad a escalas próximas al tamaño de las células que intervienen en el proceso de adhesión