Analytical Solution for Bending and Free Vibrations of an Orthotropic Nanoplate based on the New Modified Couple Stress Theory and the Third-order Plate Theory

In the present work, the equations of motion of a thin orthotropic nanoplate were obtained based on the new modified couple stress theory and the third-order shear deformation plate theory. The nanoplate was considered as a size-dependent orthotropic plate. The governing equations were derived using the dynamic version of Hamilton’s principle and natural boundary conditions were formulated. An analytical solution in the form of a double Fourier series was obtained for a simply supported rectangular nanoplate. The eigenvalue problem was set and solved. It was analytically shown that the displacements of the median surface points in the plane of the plate do not depend on the material length scale parameters in the same directions; these in-plane directional displacements depend on the material length scale parameter in the out-of-plane direction only. On the other hand, the out-of-plane directional displacement depends on the length scale parameter in the plane directions only. The cross-section rotation angles depend on all length scale parameters. It was shown that the size-dependent parameters only have a noticeable effect on the deformed state of the plate if their order is not less than the order (plate height)-1

Analytical Solution for Bending and Free Vibrations of an Orthotropic Nanoplate based on the New Modified Couple Stress Theory and the Third-order Plate Theory

In the present work, the equations of motion of a thin orthotropic nanoplate were obtained based on the new modified couple stress theory and the third-order shear deformation plate theory. The nanoplate was considered as a size-dependent orthotropic plate. The governing equations were derived using the dynamic version of Hamilton’s principle and natural boundary conditions were formulated. An analytical solution in the form of a double Fourier series was obtained for a simply supported rectangular nanoplate. The eigenvalue problem was set and solved. It was analytically shown that the displacements of the median surface points in the plane of the plate do not depend on the material length scale parameters in the same directions; these in-plane directional displacements depend on the material length scale parameter in the out-of-plane direction only. On the other hand, the out-of-plane directional displacement depends on the length scale parameter in the plane directions only. The cross-section rotation angles depend on all length scale parameters. It was shown that the size-dependent parameters only have a noticeable effect on the deformed state of the plate if their order is not less than the order (plate height)-1

Immune-microbiota interaction in Finnish and Russian Karelia young people with high and low allergy prevalence

Background After the Second World War, the population living in the Karelian region was strictly divided by the "iron curtain" between Finland and Russia. This resulted in different lifestyle, standard of living, and exposure to the environment. Allergic manifestations and sensitization to common allergens have been much more common on the Finnish compared to the Russian side. Objective The remarkable allergy disparity in the Finnish and Russian Karelia calls for immunological explanations. Methods Young people, aged 15-20 years, in the Finnish (n = 69) and Russian (n = 75) Karelia were studied. The impact of genetic variation on the phenotype was studied by a genome-wide association analysis. Differences in gene expression (transcriptome) were explored from the blood mononuclear cells (PBMC) and related to skin and nasal epithelium microbiota and sensitization. Results The genotype differences between the Finnish and Russian populations did not explain the allergy gap. The network of gene expression and skin and nasal microbiota was richer and more diverse in the Russian subjects. When the function of 261 differentially expressed genes was explored, innate immunity pathways were suppressed among Russians compared to Finns. Differences in the gene expression paralleled the microbiota disparity. High Acinetobacter abundance in Russians correlated with suppression of innate immune response. High-total IgE was associated with enhanced anti-viral response in the Finnish but not in the Russian subjects. Conclusions and clinical relevance Young populations living in the Finnish and Russian Karelia show marked differences in genome-wide gene expression and host contrasting skin and nasal epithelium microbiota. The rich gene-microbe network in Russians seems to result in a better-balanced innate immunity and associates with low allergy prevalence.Peer reviewe

The perception of Old Norse literature in modern Icelandic children’s literature

In Icelandic children’s literature of the years 2000−2010, the texts that make use of the subjects of Old Norse literature are not very common, but their importance is undeniable. In modern culture of Iceland, the ancient literature has a high status of the “great cultural heritage” and is also extremely important in constructing the Icelandic national identity. Due to the fact that the Icelandic language has not changed considerably during the centuries, the ancient texts are read by Icelanders in the original language with comments, while younger generations may need some other ways to become familiar with the “classical” plots and characters. The modern Icelandic childern’s literature on Old Norse matters includes a great number of long and short prose texts but hardly any poems. There are mainly two genres: the mythological songs of Edda and the sagas of Icelanders (and there is also at least one example of using the kings’ sagas, namely the Saga of Ólafur Tryggvason). The texts are mainly addressed to children of the age of 9 to 13. Such children’s books have a double aim: a) the representation of the Old Norse universe itself, b) the representation of the recipient, who is supposed to be a child interested in culture and history of Iceland. Bringing the Old Norse subject to young readers can be achieved by various ways. It can be a simple retelling of a saga or a myth adapted to the perception of a modern reader. In such cases, many things concerning the realia and the worldview of the ancient age require explanation; the facts are usually never altered, even those which are not wholly in line with modern ethics. An ancient plot or person can be placed in the context of modern daily life, with e.g. Thor or Loki becoming schoolchildren. A sci-fi pattern can be employed, e.g. when a modern child finds his way into the Old Norse universum and the realm of gods. Alternativelly, a modern child can come in touch with some Old Norse realia in the modern world, such as archeological fi nds or ancient inscriptions. The high status of the literary heritage often brings about a distance between the text and its modern recipient, which may not always be desirable when the younger generation is concerned. The authors of modern Icelandic children’s books seek to reduce this distance. The contrast between the Old Norse and the modern culture, in particular the ethical, can be a special subject for discussion in such books because children’s literature is supposed to educate the readers not only about the past ages but fi rst and foremost about the world they live in

The Role of Cyclomodulins and Some Microbial Metabolites in Bacterial Microecology and Macroorganism Carcinogenesis

A number of bacteria that colonize the human body produce toxins and effectors that cause changes in the eukaryotic cell cycle—cyclomodulins and low-molecular-weight compounds such as butyrate, lactic acid, and secondary bile acids. Cyclomodulins and metabolites are necessary for bacteria as adaptation factors—which are influenced by direct selection—to the ecological niches of the host. In the process of establishing two-way communication with the macroorganism, these compounds cause limited damage to the host, despite their ability to disrupt key processes in eukaryotic cells, which can lead to pathological changes. Possible negative consequences of cyclomodulin and metabolite actions include their potential role in carcinogenesis, in particular, with the ability to cause DNA damage, increase genome instability, and interfere with cancer-associated regulatory pathways. In this review, we aim to examine cyclomodulins and bacterial metabolites as important factors in bacterial survival and interaction with the host organism to show their heterogeneous effect on oncogenesis depending on the surrounding microenvironment, pathological conditions, and host genetic background

The Fate and Functionality of Alien tRNA Fragments in Culturing Medium and Cells of <i>Escherichia coli</i>

Numerous observations have supported the idea that various types of noncoding RNAs, including tRNA fragments (tRFs), are involved in communications between the host and its microbial community. The possibility of using their signaling function has stimulated the study of secreted RNAs, potentially involved in the interspecies interaction of bacteria. This work aimed at identifying such RNAs and characterizing their maturation during transport. We applied an approach that allowed us to detect oligoribonucleotides secreted by Prevotella copri (Segatella copri) or Rhodospirillum rubrum inside Escherichia coli cells. Four tRFs imported by E. coli cells co-cultured with these bacteria were obtained via chemical synthesis, and all of them affected the growth of E. coli. Their successive modifications in the culture medium and recipient cells were studied by high-throughput cDNA sequencing. Instead of the expected accidental exonucleolysis, in the milieu, we observed nonrandom cleavage by endonucleases continued in recipient cells. We also found intramolecular rearrangements of synthetic oligonucleotides, which may be considered traces of intermediate RNA circular isomerization. Using custom software, we estimated the frequency of such events in transcriptomes and secretomes of E. coli and observed surprising reproducibility in positions of such rare events, assuming the functionality of ring isoforms or their permuted derivatives in bacteria

Lacticaseibacillus paracasei: Occurrence in the Human Gut Microbiota and K-Mer-Based Assessment of Intraspecies Diversity

Alignment-free approaches employing short k-mers as barcodes for individual genomes have created a new strategy for taxonomic analysis and paved a way for high-resolution phylogeny. Here, we introduce this strategy for the Lacticaseibacillus paracasei species as a taxon requiring barcoding support for precise systematics. Using this approach for phylotyping of L. paracasei VKM B-1144 at the genus level, we identified four L. paracasei phylogroups and found that L. casei 12A belongs to one of them, rather than to the L. casei clade. Therefore, we propose to change the specification of this strain. At the genus level we found only one relative of L. paracasei VKM B-1144 among 221 genomes, complete or available in contigs, and showed that the coding potential of the genome of this &ldquo;rare&rdquo; strain allows its consideration as a potential probiotic component. Four sets of published metagenomes were used to assess the dependence of L. paracasei presence in the human gut microbiome on chronic diseases, dietary changes and antibiotic treatment. Only antibiotics significantly affected their presence, and strain-specific barcoding allowed the identification of the main scenarios of the adaptive response. Thus, suggesting bacteria of this species for compensatory therapy, we also propose strain-specific barcoding for selecting optimal strains for target microbiomes