The "Smart Ring" Experience in l'Aquila (Italy): Integrating Smart Mobility Public Services with Air Quality Indexes

This work presents the "City Dynamics and Smart Environment" activities of the Smart Ring project, a model for the smart city, based on the integration of sustainable urban transport services and environmental monitoring over a 4–5-km circular path, the "Smart Ring", around the historical center of l'Aquila (Italy). We describe our pilot experience performed during an experimental on-demand public service electric bus, "SmartBus", which was equipped with a multi-parametric air quality low-cost gas electrochemical sensor platform, "NASUS IV". For five days (28–29 August 2014 and 1–3 September 2014), the sensor platform was installed inside the SmartBus and measured air quality gas compounds (nitrogen dioxide, carbon oxide, sulfur dioxide, hydrogen sulfide) during the service. Data were collected and analyzed on the bases of an air quality index, which provided qualitative insights on the air status potentially experienced by the users. The results obtained are in agreement with the synoptic meteorological conditions, the urban background air quality reference measurements and the potential traffic flow variations. Furthermore, they indicated that the air quality status was influenced by the gas component NO 2 , followed by H 2 S, SO 2 and CO. We discuss the features of our campaign, and we highlight the potential, limitations and key factors to consider for future project designs

The impact of vehicle movement on exploitation parameters of roads and runways: a short review of the special issue

"The impact of vehicle movement on exploitation parameters of roads and runways: a short review of the special issue" Transport, 31(2), p. 127-13

Morphology and Light‐Dependent Spatial Distribution of Spin Defects in Carbon Nitride

Carbon nitride (CN) is a heterogeneous photocatalyst that combines good structural properties and a broad scope. The photocatalytic efficiency of CN is associated with the presence of defective and radical species. An accurate description of defective states—both at a local and extended level—is key to develop a thorough mechanistic understanding of the photophysics of CN. In turn, this will maximise the generation and usage of photogenerated charge carriers and minimise wasteful charge recombination. Here the influence of morphology and light-excitation on the number and chemical nature of radical defects is assessed. By exploiting the magnetic dipole-dipole coupling, the spatial distribution of native radicals in CN is derived with high precision. From the analysis an average distance in the range 1.99–2.34 nm is determined, which corresponds to pairs of radicals located approximately four tri-s-triazine units apart

A Dynamical Mass Constraint for Pre-Main-Sequence Evolutionary Tracks: The Binary NTT 045251+3016

We present an astrometric/spectroscopic orbital solution for the pre-main-sequence binary NTT 045251+3016. Our measurements for the primary and secondary masses are 1.45 +/- 0.19 M_sun and 0.81 +/- 0.09 M_sun, respectively, and 145 +/- 8 pc for the distance of the system, consistent with prior estimates for the Taurus-Auriga star-forming region. The evolutionary tracks of D'Antona & Mazzitelli (1997), Baraffe et al. (1998), and Palla & Stahler (1999) are tested against these dynamical mass measurements. Due to the intrinsic color/T_eff variation within the K5 spectral class, each pre-main-sequence model provides a mass range for the primary. The theoretical mass range derived from the Baraffe et al. (1998) tracks that use a mixing length parameter alpha=1.0 is closest to our measured primary mass, deviating between 1.3 and 1.6 sigma. The set of Baraffe et al. (1998) tracks that use alpha=1.9 deviate between 1.6 and 2.1 sigma from our measured primary mass. The mass range given by the Palla & Stahler (1999) tracks for the primary star deviate between 1.6 and 2.9 sigma. The D'Antona & Mazzitelli (1997) tracks give a mass range that deviates by at least 3.0 sigma from our derived primary mass, strongly suggesting that these tracks are inconsistent with our observation. Observations of the secondary are less constraining than those of the primary, but the deviations between the dynamical mass of the secondary and the mass inferred for the secondary from the various pre-main-sequence tracks mirror the deviations of the primary star. All of the pre-main-sequence tracks are consistent with coevality of the components of NTT 045251+3016.Comment: 27 pages, 6 figures, 5 tables -- accepted by A

Graphene and graphene oxide induce ROS production in human HaCaT skin keratinocytes: The role of xanthine oxidase and NADH dehydrogenase

The extraordinary physicochemical properties of graphene-based nanomaterials (GBNs) make them promising tools in nanotechnology and biomedicine. Considering the skin contact as one of the most feasible exposure routes to GBNs, the mechanism of toxicity of two GBNs (few-layer-graphene, FLG, and graphene oxide, GO) towards human HaCaT skin keratinocytes was investigated. Both materials induced a significant mitochondrial membrane depolarization: 72 h cell exposure to 100 \u3bcg mL 12 1 FLG or GO increased mitochondrial depolarization by 44% and 56%, respectively, while the positive control valinomycin (0.1 \u3bcg mL 121) increased mitochondrial depolarization by 48%. Since the effect was not prevented by cyclosporine-A, it appears to be unrelated to mitochondrial transition pore opening. By contrast, it seems to be mediated by reactive oxygen species (ROS) production: FLG and GO induced time- and concentration- dependent cellular ROS production, significant already at the concentration of 0.4 \u3bcg mL 121 after 24 h exposure. Among a panel of specific inhibitors of the major ROS-producing enzymes, diphenyliodonium, rotenone and allopurinol significantly reverted or even abolished FLG- or GO-induced ROS production. Intriguingly, the same inhibitors also significantly reduced FLG- or GO-induced mitochondrial depolarization and cytotoxicity. This study shows that FLG and GO induce a cytotoxic effect due to a sustained mitochondrial depolarization. This seems to be mediated by a significant cellular ROS production, caused by the activation of flavoprotein-based oxidative enzymes, such as NADH dehydrogenase and xanthine oxidase

Oxidized Nanocarbons-Tripeptide Supramolecular Hydrogels: Shape Matters!

Short peptide hydrogels are attractive biomaterials but typically suffer from limited mechanical properties. Inclusion of other nanomaterials can serve the dual purpose of hydrogel reinforcement and of conferring additional physicochemical properties (e.g., self-healing, conductivity), as long as they do not hamper peptide self-assembly. In particular, nanocarbons are ideal candidates, and their physicochemical properties have demonstrated great potential in nanocarbon-polymer gel biomaterials for tissue engineering or drug delivery. Recently, increasing interest in supramolecular hydrogels drove research also on their enhancement with nanocarbons. However, little is known on the effect of nanocarbon morphology on the self-assembly of short peptides, which are among the most popular hydrogel building blocks. In this work, three different oxidized nanocarbons (i.e., carbon nanotube or CNT as 1D material, graphene oxide sheet or GO as 2D material, and carbon nanohorn or CNH as 3D material) were evaluated for their effects on the self-assembly of the unprotected tripeptide Leu-DPhe-DPhe at physiological conditions. Supramolecular hydrogels were obtained in all cases, and viscoelastic properties were clearly affected by the nanocarbons, which increased stiffness and resistance to applied stress. Notably, self-healing behavior was observed only in the case of CNTs. Tripeptide\u2013nanotube interaction was noted already in solution prior to self-assembly, with the tripeptide acting as a dispersing agent in phosphate buffer. Experimental and in silico investigation of the interaction between peptide and CNTs suggests that the latter acts as nucleation templates for self-assembly and reassembly. Overall, we provide useful insights for the future design of composite biomaterials with acquired properties

Easy and Versatile Synthesis of Bulk Quantities of Highly Enriched 13C-Graphene Materials for Biological and Safety Applications

The preparation of bulk quantities of 13C-labeled graphene materials is relevant for basic investigations and for practical applications. In addition, 13C-labeled graphene materials can be very useful in biological and environmental studies, as they may allow the detection of graphene or its derivatives in cells or organs. In this paper, we describe the synthesis of 13C-labeled graphene materials (few-layer graphene, FLG, and graphene oxide, GO) on a tens of mg scale, starting from 13C-labeled methane to afford carbon fibers, followed by liquid-phase exfoliation (FLG) or oxidation (GO). The materials have been characterized by several analytical and microscopic techniques, including Raman and nuclear magnetic resonance spectroscopies, thermogravimetric analysis, X-ray photoelectron spectroscopy, and X-ray powder diffraction. As a proof of concept, the distribution of the title compounds in cells has been investigated. In fact, the analysis of the 13C/12C ratio with isotope ratio mass spectrometry (IRMS) allows the detection and quantification of very small amounts of material in cells or biological compartments with high selectivity, even when the material has been degraded. During the treatment of 13C-labeled FLG with HepG2 cells, 4.1% of the applied dose was found in the mitochondrial fraction, while 4.9% ended up in the nuclear fraction. The rest of the dose did not enter into the cell and remained in the plasma membrane or in the culture media.La preparación de cantidades a granel de materiales de grafeno marcados con 13C es relevante para investigaciones básicas y para aplicaciones prácticas. Además, los materiales de grafeno marcados con 13C pueden ser muy útiles en estudios biológicos y medioambientales, ya que pueden permitir la detección de grafeno o sus derivados en células u órganos. En este trabajo describimos la síntesis de materiales de grafeno marcados con 13C (grafeno de pocas capas, FLG, y óxido de grafeno, GO) a escala de decenas de mg, partiendo de metano marcado con 13C para obtener fibras de carbono, seguidas de exfoliación en fase líquida (FLG) u oxidación (GO). Los materiales se han caracterizado mediante varias técnicas analíticas y microscópicas, como las espectroscopias Raman y de resonancia magnética nuclear, el análisis termogravimétrico, la espectroscopia de fotoelectrones de rayos X y la difracción de rayos X en polvo. Como prueba de concepto, se ha investigado la distribución de los compuestos del título en las células. De hecho, el análisis de la relación 13C/12C con espectrometría de masas de relación isotópica (IRMS) permite la detección y cuantificación de cantidades muy pequeñas de material en células o compartimentos biológicos con alta selectividad, incluso cuando el material se ha degradado. Durante el tratamiento de FLG marcado con 13C con células HepG2, el 4,1% de la dosis aplicada se encontró en la fracción mitocondrial, mientras que el 4,9% acabó en la fracción nuclear. El resto de la dosis no entró en la célula y permaneció en la membrana plasmática o en el medio de cultivo