DETECTION OF HEAVY METALS FROM MOLLUSCS SHELLS

This work presents aspects regarding some heavy metals (Cd, Pb, Cu and Zn) concentration in shells of different molluscs (Mytilus galloprovincialis, Rapana thomasiana, Mya arenaria, Scapharca) collected from the Romanian Black Sea Coast. The heavy metals were analysed using atomic absorption spectrometry

INVESTIGATION OF HEAVY METALS FROM SOME ALIMENTARY PRODUCTS

The presence in high amount of organic pollutants and heavy metals in environmentrepresents a potential danger for human health and for environment too. This workpresents aspects regarding the pollution with heavy metals (Cd, Pb, Cu and Zn) of some food products: caw milk, caw cheese and caw meat. The samples analysed were collected from some housekeeping of Lumina (Constanta). The heavy metals were analysed using atomic absorption spectrometry

Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics

MicroRNAs (miRNAs) are small non-coding endogenous RNAs, which are attracting a growing interest as therapeutic molecules due to their central role in major diseases. However, the transformation of these biomolecules into drugs is limited due to their unstability in the bloodstream, caused by nucleases abundantly present in the blood, and poor capacity to enter cells. The conjugation of miRNAs to nanoparticles (NPs) could be an effective strategy for their clinical delivery. Herein, the engineering of non-liposomal lipid nanovesicles, named quatsomes (QS), for the delivery of miRNAs and other small RNAs into the cytosol of tumor cells, triggering a tumor-suppressive response is reported. The engineered pH-sensitive nanovesicles have controlled structure (unilamellar), size (24 weeks), and are prepared by a green, GMP compliant, and scalable one-step procedure, which are all unavoidable requirements for the arrival to the clinical practice of NP based miRNA therapeutics. Furthermore, QS protect miRNAs from RNAses and when injected intravenously, deliver them into liver, lung, and neuroblastoma xenografts tumors. These stable nanovesicles with tunable pH sensitiveness constitute an attractive platform for the efficient delivery of miRNAs and other small RNAs with therapeutic activity and their exploitation in the clinics

Synthèse de sondes du transporteur des sucres chez Plasmodium falciparum

rédigé en 2006The malaria, the disease provoked by the parasite Plasmodium, kills every year 1,5 to 2,7 million persons in the world. The discovery of a specific protein (PfHT) responsible for the transport of the sugar necesssary for the parasite to develop within the red corpuscles which it infects, lets envisage that this protein can become a new therapeutic target.The present work concerned the preparation of around thirty by-products of the glucose variously substituted in -3, to know better the influence of the nature, as well as the hydrophilie or the hydrophobia of these substituants, on the inhibition of the protein of transport of sugars at Plasmodium.Besides, same substituting (the motive undec-10-enyle) was introduced in a systematic way on the other positions of the glucose, both on oxygens and on carbons.Le paludisme, maladie provoquée par le parasite Plasmodium, tue chaque année de 1,5 à 2,7 millions de personnes dans le monde. La découverte d'une protéine spécifique (PfHT) responsable du transport du sucre nécessaire au parasite pour se développer au sein des globules rouges qu'il infecte, laisse envisager que cette protéine puisse devenir une nouvelle cible thérapeutique.Le présent travail a concerné la préparation d'une trentaine de dérivés du glucose diversement substitués en -3, afin de mieux connaître l'influence de la nature, ainsi que de l'hydrophilie ou de l'hydrophobie de ces substituants, sur l'inhibition de la protéine de transport des sucres chez Plasmodium. Par ailleurs, un même substituant (le motif undec-10-enyle) a été introduit de façon systématique sur les autres positions du glucose, tant sur les oxygènes que sur les carbones

Synthèse de sondes du transporteur des sucres chez Plasmodium falciparum

Le paludisme, maladie provoquée par le parasite Plasmodium, tue chaque année de 1,5 à 2,7 millions de personnes dans le monde. La découverte d'une protéine spécifique (PfHT) responsable du transport du sucre nécessaire au parasite pour se développer au sein des globules rouges qu'il infecte, laisse envisager que cette protéine puisse devenir une nouvelle cible thérapeutique. Le présent travail a concerné la préparation d'une trentaine de dérivés du glucose diversement substitués en -3, afin de mieux connaître l'influence de la nature, ainsi que de l'hydrophilie ou de l'hydrophobie de ces substituants, sur l'inhibition de la protéine de transport des sucres chez Plasmodium. Par ailleurs, un même substituant (le motif undec-10-enyle) a été introduit de façon systématique sur les autres positions du glucose, tant sur les oxygènes que sur les carbones.GRENOBLE1-BU Sciences (384212103) / SudocSudocFranceF

Palladium-Catalysed C2 or C5 Direct Arylation of 3-Substituted Thiophenes with Aryl Bromides

International audienceThe Pd(OAc)2/dppb system was found to be an efficient catalyst for the direct arylation of 3-substituted thiophene derivatives. The regioselectivity of the arylation strongly depends on the thiophene substituent and also on the nature of the aryl bromide. When using 3-formyl, 3-cyano, 3-methyl, 3-hydroxymethyl or 3-bromothiophene, the 2-arylated thiophenes were obtained with 76-95% regioselectivity; whereas, the arylation of 3-formylthiophene diethylacetal or 3-acetylthiophene gave the 5-arylated thiophenes with 52-90% regioselectivity. The use of congested aryl bromides favours the arylation at C5. These reactions were performed using only 0.1 mol% of catalyst. Moreover, this procedure has been found to be tolerant to a variety of functional groups on the aryl bromide such as formyl, propionyl, benzoyl, nitrile, and nitro

Correlation between thermostability and stability of glycosidases in ionic liquid

International audienceThe activity and stability of a β-glycosidase ( and two α-galactosidases ( and were studied in different hydrophilic ionic liquid (IL)/water ratios. For the ILs used, the glycosidases showed the best stability and activity in 1,3-dimethylimidazolium methyl sulfate [MMIM][MeSO] and 1,2,3-trimethylimidazolium methyl sulfate [TMIM][MeSO]. A close correlation was observed between the thermostability of the enzymes and their stability in IL media. At high IL concentration (80%), a time-dependent irreversible denaturing effect was observed on glycosidases while, at lower concentration (<30%), a reversible inactivation affecting mainly the was obtained. The results demonstrate that highly thermostable glycosidases are more suitable for biocatalytic reactions in water-miscible ILs