Gait analysis for challenged users based on a rollator equipped with force sensors

El análisis del paso proporciona información detallada acerca del estado de los pacientes, su progreso en los tratamientos de rehabilitación y el riesgo de caídas que tienen. Este análisis se realiza usualmente en condiciones muy específicas, haciendo uso de cintas de andar, cámaras y/o sensores vestibles. En el artículo presentado en esta conferencia, se propone un análisis del paso usando un andador inteligente, equipado con un conjunto básico de sensores. Este trabajo propone una metodología para obtener los parámetros relevantes del paso utilizando una herramienta común y de bajo coste: odometría de las ruedas de un andador y sensores de fuerza del manillar. Las principales ventajas de este enfoque es que los usuarios no son molestados con sensores adicionales en su cuerpo. Además, el seguimiento se puede realizar en cualquier lugar, incluso haciendo las actividades de la vida cotidiana y por períodos de tiempo prolongados. Hemos probado nuestro sistema con un conjunto de pacientes de rehabilitación con diferentes discapacidades. Los resultados preliminares han demostrado con éxito que los parámetros extraídos son coherentes con los efectos reportados por el equipo médico sobre su condición.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Ectomycorrhizal status of a mature productive black truffle plantation

The truffle-plantation «Los Quejigares» was planted in 1971 by AROTZ-CATESA company. It is a 600 ha plot of Quercus ilexmycorrhizated with Tuber melanosporum at 1,250 m a.s.l. on calcareous soil. This plantation is the largest of the world and one of the eldest truffle-plantations of Spain and it is in full production. Knowledge of the mycorrhizal status of a mature black truffle plantation is significant for the improvement of truffle cultivation. Ectomycorrhizae were studied for knowing T. melanosporum persistence and diversity and abundance of other ectomycorrhizal types. Roots of 16 holmoaks were sampled, 12 trees produce truffle sporocarps and four did not. It was found a mean of about 35% of T. melanosporum ectomycorrhizae in the studied trees, being this significantly higher in the productive trees. Also, 105 more different ectomycorrhizal types were found. In spite of the high number of morphotypes found, it seems that they do not replace T. melanosporum, showing that there is a coexistence between species in the fungal community associated to the roots

Caracterización de ectomicorrizas en encinares productores de trufa negra del noreste de Soria

Black truffle sporocarp production is determined, amongst many other factors, by an optimum mycorrhization degree in the roots of the host-tree. This process is triggered in balance with other fungal species that does not inhibit its sporocarp production. So, there is an ectomycorrhizal fungal community associated with the black truffle sporocarp productive host-trees that runs as any other living beings community, producing a certain fungal biodiversity and establishing connections in dynamic balance that will evolve. The absence of black truffle sporocarps production in some host-trees will be determined by its ectomycorrhiza absence, or because between the fungal community there are one or more species that inhibit this process or displace it. With the aim of study those topics, the ectomycorrhizae present in 23 adult holmoak trees (Quercus ilex subsp. ballota (Desf.) Samp.) of seven Tuber melanosporum Vittad. productive areas in the North-West of Soria (inner Spain) are characterized and quantified. During the spring and the autumn of 1999 and 2000, black truffle productive and non-productive holmoaks were studied following the global method (Verlhac et al., 1990, La truffe guide pratique). Ectomycorrhizal types were characterized following the guidelines of Agerer (1999). Tuber melanosporum, T. aestivum Vittad., T. brumale Vittad., Cenococcum geophylum Fr., Pisolithus arrhizus (Scop.) Rauscher, Cantharellus tubaeformis (Bull.) Fr., Hebeloma cf. sinapizans (Fr.) Sacc., Tomentella galzinii Bourdot, AD type, Cortinarius sp., Hebeloma sp. and Scleroderma sp. and many others Telephorales, Tuberales and Boletales ectomycorrhizal types were found. Tuber melanosporum mycorrhizae are present both in productive and non-productive hosttrees, as it happens for T. aestivum, while T. brumale ectomycorrhizae are only present in nonproductive holmoaks. The rest of identified ectomycorrhizal types are present in productive and non-productive host-trees

Expression of bacterial virulence factors and cytokines during in vitro macrophage infection by enteroinvasive Escherichia coli and Shigella flexneri: a comparative study

Enteroinvasive Escherichia coli (EIEC) and Shigellaspp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigellashare many genetic and biochemical similarities, the illness caused by Shigellais more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneriwith cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed thatS. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri.FAPES

Spillover of interpersonal conflicts from work into nonwork: A daily diary study

This study among a heterogeneous sample of employees expands the Job-Demands (JD-R) theory by examining how interpersonal conflicts at work -task and relationship conflictspillover into the non-work domain on a daily basis. We hypothesized that daily personal resources can buffer the daily negative spillover of interpersonal conflicts from work into the non-work domain. A total of 113 employees (N = 565 occasions) filled in a daily diary questionnaire in the evening before bedtime over five consecutive working days. Results of multi-level analysis showed that the presence of daily personal resources is essential in order to buffer the spillover of interpersonal conflict at work to the non-work domain. Specifically, on days that employees were not very optimistic or resilient, interpersonal conflicts resulted in higher strain-based work-life conflict experiences. These findings contribute to the JD-R theory and show how the unfavorable effects of daily interpersonal conflicts in the work domain may be avoided in the non-work domain through enhancing personal resources. We discuss the implications for theory and practice

Establishment of the bacterial fecal community during the first month of life in Brazilian newborns

OBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of São Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries

Síntesis de ectomicorrizas entre Cistus sp. y las especies del complejo Boletus edulis

En los jarales resultantes de la reiteración de incendios forestales en el oeste de Castilla y León (León, Salamanca y Zamora) es habitual la presencia de fructificaciones de Boletus edulis Bull. La recolección de estos hongos micorrícicos comestibles en los jarales dominados exclusivamente por Cistus ladanifer, supone una oportunidad económica alternativa a los usos tradicionales para zonas con bajos ingresos procedentes del monte. El objetivo de éste trabajo es la obtención de ectomicorrizas de las especies del complejo B. edulis con Cistus sp. bajo condiciones controladas, y las descripciones anatómicas detalladas de las mismas. La identificación de los aislados fúngicos de Boletus aereus Bull., B. edulis, B. reticulatus Schaeff. y B. pinophilus Pilát & Dermek fue confirmada por métodos moleculares. Se han obtenido ectomicorrizas de Boletus aereus, B. edulis y B. reticulatus con Cistus albidus L. y C. ladanifer en condiciones de síntesis en cultivo puro, en un substrato de turbavermiculita estéril y solución nutritiva. Las ectomicorrizas formadas se describieron siguiendo los caracteres anatómicos y morfológicos habituales. Las tres ectomicorrizas son muy similares, de color blanco, con ramificación monopodial-pinnada, manto formado por tres capas plectenquimatosas y con rizomorfos de tipo boletoide

Estudio de potenciales parasitoides (Hymenoptera) de Drosophila suzukii (Diptera: Drosophilidae) en la provincia de Buenos Aires, Argentina

Drosophila suzukii (Matsumura) is a pest that affects a great variety of fruit crops. The objective of this work was to learn about the diversity of parasitoids (Hymenoptera) that might be associated with SWD in fruit crops located in northeastern Buenos Aires province, Argentina. Species were identified as Euxestophaga argentinensis Gallardo, Ganaspis pelleranoi (Brethes), Leptopilina boulardi (Barbotin, Carton & Kelner-Pillault), Hexacola bonaerensis Reche (Cynipoidea, Figitidae), Trichopria anastrephae (Costa Lima) (Diaprioidea, Diapriidae) and Pachycrepoideus vindemmiae (Rondani) (Chalcidoidea, Pteromalidae). Leptopilina boulardi, E. argentinensis and T. anastrephae were reported for the first time in the Buenos Aires province.Drosophila suzukii (Matsumura) es una plaga que afecta a una gran variedad de cultivos frutales. El objetivo de este trabajo fue conocer la diversidad de parasitoides (Hymenoptera) que podrían estar asociados con la SWD en cultivos frutales ubicados en el noreste de la provincia de Buenos Aires, Argentina. Las especies fueron identificadas como Euxestophaga argentinensis Gallardo, Ganaspis pelleranoi (Brethes), Leptopilina boulardi (Barbotin, Carton & Kelner-Pillault), Hexacola bonaerensis Reche (Cynipoidea, Figitidae), Trichopria anastrephae (Costa Lima) (Diaprioidea, Diapriidae) y Pachycrepoideus vindemmiae ( Rondani) (Chalcidoidea, Pteromalidae). Leptopilina boulardi, E. argentinensis y T. anastrephae fueron reportadas por primera vez en la provincia de Buenos Aires.Fil: Gallardo, Fabiana Edith. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Reche, Vanina Anadina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; ArgentinaFil: Margaría, Cecilia B.. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Centro de Investigacion En Sanidad Vegetal.; ArgentinaFil: Aquino, Daniel Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Centro de Investigacion En Sanidad Vegetal.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Ansa, María Agustina. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Dettler, Maria Antonela. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Vazquez, Florencia. Universidad Nacional de Luján. Departamento de Tecnología; ArgentinaFil: Barrientos, Gualterio Nicolás. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Santadino, Marina Vilma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Tecnología; ArgentinaFil: Martinez, Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Riquelme Virgala, María Begoña. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentin

A Comparative Study of the Outer Membrane Proteome from an Atypical and a Typical Enteropathogenic Escherichia coli

This study compared the proteomic profile of outer membrane proteins (OMPs) from one strain of atypical enteropathogenic Escherichia coli (aEPEC) and one of typical EPEC (tEPEC). The OMPs fractions were obtained using sarcosine extraction, and analyzed by one- and two-dimensional gel electrophoresis (1DE and 2DE, respectively). The 1DE OMPs analysis of typical and atypical EPEC evidenced similar patterns; however, the 2DE OMP profile from the aEPEC revealed more protein spots in the 40- to 70-kDa region. 2DE image analysis identified 159 protein spots in both strains whereas 53 protein spots were observed only in tEPEC and 128 were observed only in aEPEC. Remarkably, 41.5% of aEPEC spots showed higher levels of expression compared to tEPEC, some of which with two, others four or even five times more. Twenty-four selected spots were identified using MALDI-TOF mass spectrometry and they corresponded to proteins involved in cell structure and metabolism, as well as in gene regulation. Some of these proteins showed similarity with proteins identified in other E. coli pathotypes. Besides, the differential expression of some proteins in aEPEC may suggest that it could be related to their features that ascertain the adaptation to distinct environments and the worldwide spread distribution of these pathogens