Modelling Nitrogen Uptake in Plants and Phytoplankton: Advantages of Integrating Flexibility into the Spatial and Temporal Dynamics of Nitrate Absorption

Under field conditions, plants need to optimize nutrient ion and water acquisition in their fluctuating environment. One of the most important variables involved in variations of ion uptake processes is temperature. It modifies the thermodynamic processes of root uptake and ion diffusion in soil throughout day–night and ontogenetic cycles. Yet, most models of nitrogen (N) uptake in plants are built from set values of microscopic kinetic parameters, Vm and Km, derived from a Michaelis–Menten (MM) interpretation of nutrient isotherms. An isotherm is a curve depicting the response of root nitrate influx to external nitrate concentrations at a given temperature. Models using the MM formalism are based on several implicit assumptions that do not always hold, such as homothetic behavior of the kinetic parameters between the different root biological scales, i.e., the epidermis cell, root segments, root axes, and the whole root system. However, in marine phytoplankton, it has been clearly demonstrated that the macroscopic behavior in the nutrient uptake of a colony cannot be confounded with the microscopic behavior of individual cells, due to the cell diffusion boundary layer. The same is also true around plant root segments. Improved N uptake models should either take into account the flexibility of the kinetic parameters of nitrate uptake at the cellular level (porter–diffusion approach) or use the more realistic macroscopic kinetic parameters proposed by the flow–force approach. Here we present recent solutions proposed in marine phytoplankton and plant nutrient uptake models to make a more flexible description of the nutrient ion uptake process. Use of the mechanistic porter–diffusion approach developed in marine phytoplankton introduces more flexibility in response to cell characteristics and physical processes driven by temperature (diffusion and convection). The thermodynamic flow–force interpretation of plant-based nutrient uptake isotherms introduces more flexibility in response to environmental cues and root aging. These two approaches could help solve many problems that modelers encounter in these two research areas

Nitrate leaching under grassland as affected by mineral nitrogen fertilization and cattle urine

International audienceAn experiment was performed to better understand to what extent nitrogen fertilization rate and date and amount of urine deposition, when acting in combination, influence nitrate leaching under grassland. Leaching was studied during two successive winters using 2-m(2) grassed lysimeters under three levels of N fertilization (0, 150, and 300 kg N ha(-1) yr(-1), referred to as ON, 150N, and 300N, respectively), two levels of N-15-labeled urine (105 and 165 kg N ha(-1), referred to as A2 and A3, respectively), and three dates of urine application (spring, summer, and fall). During the first winter, total N leaching losses varied between 2 and 50 kg N ha(-1). When tested in combination, N applied as urine to grassland resulted in three times the total N loss by leaching that occurred following N fertilization in the first winter (4.3, 20.8, 34.9, 14.2, 17.1, and 28.7 kg NO3--N ha(-1) for no urine, A2, A3, ON, 150N, and 300N, respectively). Leaching of N-15 urine significantly depended on the date of application: 6.6, 17.3, and 29.1 kg for spring, summer, and fall, respectively. A similar pattern was observed for the contribution of N-15 urine to total N leaching with 4.3, 12.9, and 21.4%. However, urine application, both in terms of amount and date, showed very little long-term effect on these N losses in Year 2. In our conditions of low winter rainfall and drainage, grazing management (through season, urinary N amounts, and urine N concentration) resulted in a higher impact on water nitrate quality than moderate N fertilization management

Fructan, sucrose and related enzyme activities are preserved in timothy (Phleum pratense L.) during wilting

Timothy (Phleum pratense L.) is an important forage grass used for pasture, hay and silage in regions with cool and humid growth seasons. Harvesting conditions could reduce its nutritive value, particularly with extended wilting periods. To understand how daytime or night-time wilting influences the nutritive value of timothy, this study investigated the metabolism of non-structural carbohydrates, including fructan and starch, together with total soluble protein and amino acid patterns in timothy plants harvested at two maturity stages (heading and anthesis) and wilted under controlled conditions for 24h at two temperatures (15 degrees C, 20 degrees C) and two light regimes (darkness, light) by simulating different wilting management practices. Correlation analysis with the whole dataset showed that soluble protein, glucose and starch contents declined in plant tissues concomitantly with water loss, while amino acid, sucrose and fructose contents increased. Transient increase in amino acid content suggests that the decrease in protein content was due to proteolysis during wilting. Sucrose and fructose contents generally increased in plant tissues harvested at anthesis and wilted in light whereas they were unaffected in plants wilted in darkness. Fructan content remained stable. Fructan exohydrolase (FEH) and soluble acid invertase (INV) activities were well preserved during the first 12h of wilting and might facilitate the fermentation process at the beginning of ensiling by supplying fructose from fructans and hexoses from sucrose to the fermentive bacteria

Water‐soluble carbohydrates in Patzkea paniculata (L.): a plant strategy to tolerate snowpack reduction and spring drought in subalpine grasslands

International audienceIn subalpine grasslands of the central French Alps, cessation of traditional mowing promotes dominance of Patzkea paniculata (L.) G.H.Loos (Poaceae) tussocks, with high biomass but low fodder quality. Mowing limits P. paniculata abundance through the depletion of its water-soluble carbohydrate (WSC) reserves, which sustain early spring growth initiation. However, the effectiveness of mowing effects is modulated by grassland functional composition, fertilization and climate change, as WSC compounds, and notably fructans, support plant physiological responses to climate stresses such as drought or frost. • To characterize the mechanisms underpinning the control of P. paniculata under global change, we tested the effects of climate manipulation (combined snow removal and drought) and management (cutting and fertilization) alone or in combination on P. paniculata WSC storage in assembled grassland communities of varying functional composition. • Management and climate treatments individually decreased seasonal fructan storage, with neither additive nor synergic effects between them, primarily due to the dominance of management over climate effects. Fructan amounts were higher in individuals growing in unmanaged exploitative communities compared to unmanaged conservative communities, regardless of climate treatments, but management overrode these differences. • Our findings suggest that reduction by combined snow removal and drought of P. paniculata carbon allocation to WSC storage may similarly limit its dominance to that in current mowing practices

Lolium perenne, backbone of sustainable development, source of fructans for grazing animals and potential source of novel enzymes for biotechnology

Chapitre 12[i]Lolium perenne[/i], backbone of sustainable development, source of fructans for grazing animals and potential source of novel enzymes for biotechnolog

Changes in (NO3-)-N-15 availability and transpiration rate are associated with a rapid diurnal adjustment of anion contents as well as N-15 and water fluxes between the roots and shoots

International audienceBackground and Aims: Understanding interactions between water and nitrate fluxes in response to nitrate availability and transpiration rate is crucial to select more efficient plants for the use of water and nitrate. Methods: Some of these interactions were investigated in intact Brassica napus plants by combining a non-destructive gravimetric device with (NO3-)-N-15 labeling. The set-up allowed high-resolution measurement of the effects of a cross-combination of two concentrations of KNO3 or KCl (0.5 and 5 mM) with two different rates of transpiration controlled by the relative humidity during a day-night cycle. Key Results: Results show that (1) high external nitrate concentrations increased root water uptake significantly whatever the transpiration rate, (2) nitrate translocation depended both on the rate of nitrate uptake and loading into xylem (3) dilution-concentration effect of nitrate in the xylem was mainly modulated by both external nitrate availability and transpiration rate, (4) dynamic changes in N-15 translocation in the xylem modified shoot growth and capacitance, and (5) variations in tissue concentrations of NO3- induced by the experimental conditions were balanced by changes in concentrations of chloride and sulfate ions. These effects were even more amplified under low transpiration condition and 0.5 mM external nitrate concentration. Conclusion: Taken together, these results highlight the fine and rapid adjustment of anion contents, nitrate and water flows to changes in transpiration rate and nitrate availability during a day-night cycle. The use of this non-invasive gravimetric device is therefore a powerful tool to assess candidates genes involved in nitrogen and water use efficiency

Plant maturity and nitrogen fertilization affected fructan metabolism in harvestable tissues of timothy (Phleum pratense L.)

International audienceTimothy (Phleum pratense L.) is an important grass forage used for pasture, hay, and silage in regions with cool and humid growth seasons. One of the factors affecting the nutritive value of this grass is the concentration of non-structural carbohydrates (NSC), mainly represented by fructans. NSC concentration depends on multiple factors, making it hardly predictable. To provide a better understanding of NSC metabolism in timothy, the effects of maturity stage and nitrogen (N) fertilization level on biomass, NSC and N-compound concentrations were investigated in the tissues used for forage (leaf blades and stems surrounded by leaf sheaths) of hydroponically grown plants. Moreover, activities and relative expression level of enzymes involved in fructan metabolism were measured in the same tissues. Forage biomass was not altered by the fertilization level but was strongly modified by the stage of development. It increased from vegetative to heading stages while leaf-to-stem biomass ratio decreased. Total NSC concentration, which was not altered by N fertilization level, increased between heading and anthesis due to an accumulation of fructans in leaf blades. Fructan metabolizing enzyme activities (fructosyltransferase-FT and fructan exohydrolase-FEH) were not or only slightly altered by both maturity stage and N fertilization level. Conversely, the relative transcript levels of genes coding for enzymes involved in fructan metabolism were modified by N supply (PpFT1 and Pp6-FEH1) or maturity stage (PpFT2). The relative transcript level of PpFT1 was the highest in low N plants while that of Pp6-FEH1 was the highest in high N plants. Morevoer, transcript level of PpFT1 was negatively correlated with nitrate concentration while that of PpFT2 was positively correlated with sucrose concentration. This distinct regulation of the two genes coding for 6-sucrose: fructan fructosyltransferase (6-SFT) may allow a fine adequation of C allocation towards fructan synthesis in response to carbon and N availability. Contrary to fructans, starch content increased in low N plants, suggesting different regulatory mechanisms and/or sensitivity of starch and fructan metabolism in relation to the N status

Diagnostic et déterminants de la pérennité des prairies. Sol et pérennité des prairies : un cercle vertueux

International audienceThe physical, chemical and biological properties of the soil will affect plant growth and condition the sustainability of the grasslands. Thus, soil compaction due to grazing or machine traffic will result in a loss of yield, the effects being variable according to texture (more pronounced on more clayey soils) and season. On the other hand, the preservation of soil organisms and associated functions will contribute to increasing yields. On the other hand, grassland management will also impact soil properties. Thus, the introduction of grassland in the crop rotation will quickly allow, from the second year, to favor soil biodiversity (fauna, microorganisms) and their activities. More broadly, the introduction of grassland into an annual crop rotation positively influences the maintenance of soil structure and the conservation of biodiversity, without modifying water regulation. The positive effect inherited from grasslands in the rotation can be observed even three years after recultivation. The sustainability of the grasslands will be conditioned by several factors: the duration of the grassland in the rotation, grazing management (load, period), fertilization and plant diversity. Levers exist to ensure the sustainability of these temporary or permanent grassland systems.Les propriétés physiques, chimiques et biologiques des sols vont agir sur la croissance des plantes et conditionner la pérennité des prairies. Ainsi, la compaction d’un sol, sous l’action du pâturage ou du trafic d’engins, va aboutir à une perte de rendement, les effets étant variables selon la texture (plus marqués sur des sols plus argileux) et la saison. A contrario, la préservation des organismes des sols et des fonctions associées va contribuer à augmenter les rendements. En contrepartie, la gestion des prairies va aussi impacter les propriétés des sols. Ainsi, l’introduction de prairie dans la rotation culturale, va permettre rapidement dès la deuxième année de favoriser la biodiversité des sols (faune, microorganismes) et leurs activités. Plus largement, l’introduction de prairies dans une rotation de cultures annuelles influence positivement le maintien de la structure du sol et la conservation de la biodiversité, sans modifier la régulation hydrique. L’effet positif hérité de prairies dans la rotation est observable même trois années après la remise en culture. La pérennité des prairies va être conditionnée par plusieurs facteurs : la durée de prairie dans la rotation, la gestion du pâturage (chargement, période), la fertilisation et la diversité végétale. Des leviers existent pour assurer la durabilité de ces systèmes prairiaux temporaires ou permanents.Cet article de la revue Fourrages, est édité par l'Association Francophone pour les Prairies et les Fourrages Pour toute recherche dans la base de données et pour vous abonner : www.afpf-asso.fr Association Francophone pour les Prairies et les Fourrages AFPF-Maison Nationale des Eleveurs-149 rue de Bercy-75595 Paris Cedex 1

Grazing intensity modulates carbohydrate storage pattern in five grass species from temperate grasslands

Regrowth after defoliation is an essential mechanism of plant tolerance to grazing. In grasses, non-structural carbohydrates (NSC) contained in tiller bases constitute a major substrate for regrowth after defoliation. Using a multi-specific approach, the present study aimed at testing the effect of grazing intensity on NSC concentration in tiller bases. We selected five grazing-tolerant grass species (Agrostis stolonifera, Cynosurus cristatus, Hordeum secalinum, Lolium perenne and Poa trivialis) and collected plants in a grassland subjected to two cattle grazing intensities (intensive versus moderate) for years. We measured NSC concentrations (starch, fructans, sucrose, glucose and fructose) in tiller bases. We found that fructan and sucrose concentrations before the grazing season (April) were higher under intensive than moderate grazing. By contrast, no significant effect of the grazing intensity on these NSC concentrations in tiller bases remained at the end of the grazing season (October). These results suggest that the level of reserves available before the onset of disturbance caused by grazing as well as the reserve replenishment capacity during the grazing season are modified by the intensity of grazing

Effects of elevated CO₂ and extreme climatic events on forage quality and in vitro rumen fermentation in permanent grassland

International audienceAbstract. The aim of this study was to analyze changes in botanical and chemical composition, as well as in vitro rumen fermentation characteristics of an upland grassland exposed to climate changes in controlled CO2 concentration, air temperature and precipitation conditions. Grassland was exposed to a future climate scenario coupled with CO2 treatments (390 and 520 ppm) from the beginning of spring. During summer, an extreme climatic event (ECE; 2 weeks of a +6 ∘C increase in temperature, together with severe drought) was applied and then followed by a recovery period. Three cutting dates were considered, i.e. in April, June and November. The results indicate that increases in greenness, nitrogen (N) content and changes in water-soluble carbohydrate profile in association with botanical composition changes for the November cut lead to higher in vitro dry matter degradability (IVDMD) in the rumen. The neutral detergent fiber : nitrogen (NDF:N) ratio appeared to be a key driver of forage quality, which was affected in opposite ways by elevated CO2 and ECE, with a strong impact on rumen fermentation. Atmospheric CO2 concentration in interaction with ECE tended to affect IVDMD, indicating that the effects of elevated CO2 and ECE may partly offset each other. Our findings indicate that the various factors of climate change need to be considered together in order to properly characterize their effects on forage quality and use by ruminants