ROLE OF THE NEURONAL SPECIFIC TRANSCRIPTION COREGULATOR NPDC-1 IN RETINOID AND THYROID RECEPTOR SIGNALING IN HUMAN AND THE AXOLOTL AMBYSTOMA MEXICANUM

Section I: This section is an introduction to the field of nuclear receptors. A general overview of nuclear receptor-mediated transcriptional regulation is followed by a review of literature on retinoid and thyroid receptor-mediated signaling. Section II: An introduction to NPDC-1 (neural proliferation, differentiation, and control), its discovery and characterization with regards to developmental expression and cellular localization. In addition NPDC-1 has been found to associate with a number of cell cycle regulatory proteins. NPDC-1 is characterized as a regulator of nuclear receptor-mediated transcriptional regulation. NPDC-1 was also demonstrated to be regulated post-transcriptionally through the ubiquitin/proteosome degradation pathway. Section III: Axolotl NPDC-1 (aNPDC-1) was cloned from axolotl brain and analyzed for homology to NPDC-1 from higher vertebrates. The tissue distribution and developmental expression of axolotl NPDC-1 were also examined. Section IV: The axolotl homolog for RAR (aRAR) was isolated from axolotl brain. Axolotl NPDC-1 and aRAR were then examined in a series of assays for interactions. Axolotl NPDC-1 was found to repress transcription mediated by aRAR to a smaller extent than the repression observed in higher vertebrates. The DNA binding of aRAR-RXR was increased in the presence of aNPDC-1 and complex mobility was also observed. The domain of interaction between aNPDC-1, aRAR and hRXR was localized in the amino terminus of aNPDC-1. Axolotl NPDC-1 was also demonstrated to repress proliferation as measured by reduced [3H] thymidine incorporation. Section V: The axolotl homologs of TR and TR (aTR) genes were isolated and utilized in a series of experiments to demonstrate an interaction between aTRs and aNPDC-1. As observed for RE, aNPDC-1 increases the binding of aTR-RXR heterodimer to xDR4, but no change in the mobility of the complex was observed. Interaction between aNPDC-1, aTR and aTR was localized to the amino terminus of aNPDC-1. In contrast to previous observations for other nuclear receptors, aNPDC-1 was found to stimulate transcription mediated by axolotl TRs, suggesting a role for aNPDC-1 in axolotl metamorphosis. Section VI: A summary of data presented in the previous sections as well as a presentation of future directions and a proposed model for NPDC-1 actions in retinoid and thyroid-receptor mediated signaling in axolotl

A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

BACKGROUND: The specificity of a nuclear receptor's ability to modulate gene expression resides in its ability to bind a specific lipophilic ligand, associate with specific dimerization partners and bind specific DNA sequences in the promoter regions of genes. This sequence of events appears to be the basis for targeting an additional regulatory complex composed of a variety of protein and RNA components that deliver signals for facilitation or inhibition of the RNA polymerase complex. Characterization of the tissue and cell-specific components of these coregulatory complexes appear to be integral to our understanding of nuclear receptor regulation of transcription. RESULTS: A novel yeast screen sensitive to retinoid-X receptor (RXR) transcriptional activation resulted in the isolation of the rat homologue of the mouse NPDC-1 gene. NPDC-1 has been shown to be involved in the control of neural cell proliferation and differentiation, possibly through interactions with the cell cycle promoting transcription factor E2F-1. Although the amino acid sequence of NPDC-1 is highly conserved between mouse, rat and human homologues, their tissue specific expression was seen to vary. A potential for direct protein:protein interaction between NPDC-1, RXR and retinoic acid receptor beta (RARβ) was observed in vitro and NPDC-1 facilitated RXR homodimer and RAR-RXR heterodimer DNA binding in vitro. Expression of NPDC-1 was also observed to repress transcription mediated by retinoid receptors as well as by several other nuclear receptor family members, although not in a universal manner. CONCLUSIONS: These results suggest that NPDC-1, through direct interaction with retinoid receptors, functions to enhance the transcription complex formation and DNA binding function of retinoid receptors, but ultimately repress retinoid receptor-mediated gene expression. As with NPDC-1, retinoids and their receptors have been implicated in brain development and these data provide a point of convergence for NPDC-1 and retinoid mediation of neuronal differentiation

Urine in bioelectrochemical systems: An overall review

In recent years, human urine has been successfully used as an electrolyte and organic substrate in bioelectrochemical systems (BESs) mainly due of its unique properties. Urine contains organic compounds that can be utilised as a fuel for energy recovery in microbial fuel cells (MFCs) and it has high nutrient concentrations including nitrogen and phosphorous that can be concentrated and recovered in microbial electrosynthesis cells and microbial concentration cells. Moreover, human urine has high solution conductivity, which reduces the ohmic losses of these systems, improving BES output. This review describes the most recent advances in BESs utilising urine. Properties of neat human urine used in state‐of‐the‐art MFCs are described from basic to pilot‐scale and real implementation. Utilisation of urine in other bioelectrochemical systems for nutrient recovery is also discussed including proofs of concept to scale up systems

Iron oxide nanoflowers encapsulated in thermosensitive fluorescent liposomes for hyperthermia treatment of lung adenocarcinoma

Magnetic hyperthermia (MHT) is in the spotlight of nanomedical research for the treatment of cancer employing magnetic iron oxide nanoparticles and their intrinsic capability for heat dissipation under an alternating magnetic field (AMF). Herein we focus on the synthesis of iron oxide nanoflowers (Nfs) of different sizes (15 and 35 nm) and coatings (bare, citrate, and Rhodamine B) while comparing their physicochemical and magnetothermal properties. We encapsulated colloidally stable citrate coated Nfs, of both sizes, in thermosensitive liposomes via extrusion, and RhB was loaded in the lipid bilayer. All formulations proved hemocompatible and cytocompatible. We found that 35 nm Nfs, at lower concentrations than 15 nm Nfs, served better as nanoheaters for magnetic hyperthermia applications. In vitro, magnetic hyperthermia results showed promising therapeutic and imaging potential for RhB loaded magnetoliposomes containing 35 nm Nfs against LLC and CULA cell lines of lung adenocarcinoma

Identifying jumps in financial assets: A comparison between nonparametric jump tests

We perform a comprehensive Monte Carlo comparison between nine alternative procedures available in the literature to detect jumps in financial assets using high-frequency data. We evaluate size and power properties of the procedures under alternative sampling frequencies, persistence in volatility, jump size and intensity, and degree of contamination with microstructure noise. The overall best performance is shown by the Andersen, Bollerslev, and Dobrev (2007) and Lee and Mykland (2008) intraday procedures (ABD-LM), provided the price process is not very volatile. We propose two extensions to the existing battery of tests. The first regards the finite sample improvements based on simulated critical values for the ABD-LM procedure. The second regards a procedure that combines frequencies and tests able to reduce the number of spurious jumps. Finally, we report an empirical analysis using real high frequency data on five stocks listed in the New York Stock Exchange

Guidelines for setting-up transdisciplinary sustainability courses

This report, developed by the ERASMUS+ project EUSTEPs (Enhancing Universities’ Sustainability TEaching and Practices), presents Guidelines for the creation of a Sustainability Course. It proposes a transdisciplinary curricular unit that can be included within all University degree programs (both bachelor and master). The document includes possible reasons, procedures, contents, and opportunities connected to such didactic initiative, with the aim of creating an approach that can be replicated in many Universities around the world interested in its implementation. It builds on the multi-year experience of the University of Siena (Italy) as a reference point, and the positive feedback from the EUSTEPs’ academic consortium members (namely Aristotle University of Thessaloniki, University of Aveiro and Universidade Aberta). Thanks to its flexibility, every University can interpret the proposal presented in this report in a different way and proceed according to its own preferences, conditions, knowledge, and rules.ERASMUS+, KA203 2019-2022, Agreement No. 2019-1-ELO1-KA203-062941info:eu-repo/semantics/publishedVersio

EUSTEPs administrative staff teaching module

Enhancing Universities’ Sustainability TEaching and Practices – EUSTEPs – is a project financed by ERASMUS+ program that features the collaboration among four European Universities and one Non-Governmental Organization. The team is coordinated by Aristotle University of Thessaloniki (AUTh), and is comprised of members from University of Siena (UNISI), Italy, University of Aveiro (UAv) and Universidade Aberta (UAb), both in Portugal, and Global Footprint Network (GFN), California, USA. The project aims to introduce a broader and holistic approach to sustainability within universities, having developed already one module dedicated to students and another for educators. In addition to this purpose, the project also envisions the development of a Footprint Calculator for Universities’ campus, allowing Institutions to acknowledge the environmental dimension of sustainability and connect it to their everyday functioning. All the actors whitin HEIs (students, academic staff, administrative staff and management bodies) are called to embrace a more sustainable campus, developing a set of multidisciplinary skills and a necessary shift in attitudes. This module is dedicated to the Administrative Staff of Higher Education Institutions (HEIs) and embraces a hands-on, experiential approach to sustainability understanding and Ecological Footprint concept. By presenting sustainability within the context of everyday life rather than through mere abstract theories and concepts around sustainability and, bridging with the 2030 UN Agenda Sustainable Development Goals, the administrative staff will be able to grasp how sustainability relates to not only the whole spectrum of daily life but also with their workplace and the administration of HEIs. The core aspect of the EUSTEPs Module to HEIs Administrative Staff is: 1. To make the administrative staff aware of sustainability and Ecological Footprint concepts. 2. To empower them to affect the sustainability of their workspace, as well as their community, bringing sustainability knowledge and its associated skills (usually limited to academics, researchers and students). Throughout this module, the administrative staff will not only be able to learn about sustainability and the human-environment relationship but also track their own individual Footprints; through discussing their results and behavior decisions with peers to shape a “learning by group discussion” process.ERASMUS+, KA203 2019-2022, Agreement No. 2019-1-ELO1-KA203-062941info:eu-repo/semantics/publishedVersio

“EUSTEPs Educators teaching module: sustainability around us: from theory to practice...and back”

This module, developed by the ERASMUS+ project EUSTEPs (Enhancing Universities’ Sustainability TEaching and Practices), uses a “learning-by-doing” approach to equip EU university students with science-based knowledge, multidisciplinary skills, and the transdisciplinary mindset needed to play a critical role in our societal effort towards sustainability, thus allowing students to be best prepared for the future labour market. The module is made highly effective by the adoption of the concept of Ecological Footprint (EF), a well-known and widespread quantitative approach to study, assess and understand sustainability concept. The EF is an ecology-based environmental accounting method, but we propose it not as a technical tool but as a medium to transfer important aspects of sustainability from a wide range of teachers to a wide range of students. This is possible by virtue of the verified ability of the EF method and related accessories to communicate, inform, visualize, and represent sustainability in its variegated forms. The module embraces a hands-on, experiential approach to sustainability teaching: by presenting sustainability within the context of everyday life rather than through a mere abstract teaching of intangible theories and concepts, the goal of the module is to allow students to understand, realize, and learn the full complexity of the economy-society-environment relationships, and help them grasp how sustainability relates to the whole spectrum of daily life. A series of universal, community and environmental education pedagogical approaches (PA) are employed in this module for the development of sustainability competencies. More specifically, seven out of the ten literature suggested education for sustainable development (ESD) pedagogical approaches are commissioned in this EUSTEPs teaching module, including the universal PAs of Case studies, Interdisciplinary team teaching/planning, Lecturing, Concept maps, Project or problem based learning, along with the community PA of Participatory Action Research, and the environmental education PA of Place-based environmental education.“EUSTEPs: Enhancing Universities’ Sustainability Teaching and Practices through Ecological Footprint”, KA 203, Strategic Partnership in Higher Education 2019-2022, Agreement No. 2019-1-EL01-KA203-062941.info:eu-repo/semantics/publishedVersio

UniProt-Related Documents (UniReD): assisting wet lab biologists in their quest on finding novel counterparts in a protein network.

The in-depth study of protein-protein interactions (PPIs) is of key importance for understanding how cells operate. Therefore, in the past few years, many experimental as well as computational approaches have been developed for the identification and discovery of such interactions. Here, we present UniReD, a user-friendly, computational prediction tool which analyses biomedical literature in order to extract known protein associations and suggest undocumented ones. As a proof of concept, we demonstrate its usefulness by experimentally validating six predicted interactions and by benchmarking it against public databases of experimentally validated PPIs succeeding a high coverage. We believe that UniReD can become an important and intuitive resource for experimental biologists in their quest for finding novel associations within a protein network and a useful tool to complement experimental approaches (e.g. mass spectrometry) by producing sorted lists of candidate proteins for further experimental validation. UniReD is available at http://bioinformatics.med.uoc.gr/unired/