Study of a Water-Energy Integrated System: Challenges of Prototyping

In a society where Energy and Water walk side by side in practically all sectors of activity, it becomes necessary to analyze them from a productive, economic and sustainable point of view. Although independently developed and regulated, it is known that water needs energy and energy needs water. The increasing demand for energy and its exponential trend will have repercussions at all levels in the short term. and consequently, will increase the strain on freshwater resources. All these concerns and associated decision-making policies create an opportunity to improve the management of both sectors. There is an urgent need to improve the water-energy system and reduce energy consumption, using advanced technological changes and an optimized management strategy, leading to a more sustainable future. By improving the efficiency of water and energy use through integrated smart strategies, there are very positive repercussions on savings and impact on the local economy, especially in decentralized cases. The present paper aims to address the theme of the nexus of water-energy in the scope of promoting alternatives of management and development of integrated systems and study the dynamics of the development of the theoretical concept vs. prototyping in case of urban context and real environment.info:eu-repo/semantics/publishedVersio

Development of the Concept vs Prototyping: Implementation of a real scale Water-Energy Integrated System

Currently, the concept of smart cities and renewable energy are more consolidated and developed. With each passing day, new technological advances are emerging within both themes. At the same time, the water sector has becoming increasingly demanding in terms of quality and compliance with all existing legislation. That said, it is understandable that both sectors and their management entities come together in search of more sustainable operations that go against the reduction of greenhouse gas emissions, decentralization of energy production and the improvement of efficiency, quality and use of resources. It is important to understand the synergies between water and energy to contradict what has been said so far about the increasing demand for energy, its exponential trend and the negative repercussions on freshwater resources. As such, this article aims to address the interconnection between both sectors to promote alternative scientific and technological improvements to the management and development of Integrated Water-Energy systems, while studying the dynamics between the theoretical development of the problem and the challenges of prototyping of such complex systems in real and urban context.info:eu-repo/semantics/publishedVersio

Meloidogyne exigua atacando cafeeiros no Ceará

Meloidogyne exigua Goeldi, 1887, successively recorded in the Brazilian States of Rio de Janeiro, São Paulo, Minas Gerais, Paraná and Espírito Santo, has now been observed in the State of Ceará - in the mountains of Baturité and Ibiapaba - where this species of root-knot nematode is attacking roots of cofee trees, Coffee arabica L. An interesting morphological detail was observed among the specimens collected in the Ibiapada zone: the adult females are usually large, with lengths of 500-700 µ, as compared with the standard length of 450 µ.Meloidogyne exigua Goeldi, 1887, nematóide causador de galhas em raízes de cafeeiro, Coffea arabica L., já assinalado, sucessivamente, nos Estados do Rio de Janeiro, São Paulo, Minas Gerais, Paraná e Espírito Santo, neste trabalho tem sua ocorrência registrada também no Ceará, nas serras de Baturité e da Ibiapaba, onde foi encontrado parasitando raizes de cafeeiro. Uma interessante particularidade morfológica foi observada entre os espécimens coligidos na zona da Ibiapaba: as fêmeas adultas são, em geral, grandes, excedendo o comprimento padrão referido para a espécie

Human GLB1 knockout cerebral organoids: A model system for testing AAV9-mediated GLB1 gene therapy for reducing GM1 ganglioside storage in GM1 gangliosidosis

GM1 gangliosidosis is an autosomal recessive neurodegenerative disorder caused by the deficiency of lysosomal gangliosidebeta-galactosidase (beta-gal) and resulting in accumulation of GM1 ganglioside. The disease spectrum ranges from infantile to late onset and is uniformly fatal, with no effective therapy currently available. Although animal models have been useful for understanding disease pathogenesis and exploring therapeutic targets, no relevant human central nervous system (CNS) model system has been available to study its early pathogenic events or test therapies. To develop a model of human GM1 gangliosidosis in the CNS, we employed CRISPR/Cas9 genome editing to target GLB1 exons 2 and 6, common sites for mutations in patients, to create isogenic induced pluripotent stem (iPS) cell lines with lysosomal beta-gal deficiency. We screened for clones with \u3c 5% of parental cell line beta-gal enzyme activity and confirmed GLB1 knockout clones using DNA sequencing. We then generated GLB1 knockout cerebral organoids from one of these GLB1 knockout iPS cell clones. Analysis of GLB1 knockout organoids in culture revealed progressive accumulation of GM1 ganglioside. GLB1 knockout organoids microinjected with AAV9-GLB1 vector showed a significant increase in beta-gal activity and a significant reduction in GM1 ganglioside content compared with AAV9-GFP-injected organoids, demonstrating the efficacy of an AAV9 gene therapy-based approach in GM1 gangliosidosis. This proof-of-concept in a human cerebral organoid model completes the pre-clinical studies to advance to clinical trials using the AAV9-GLB1 vector

Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation

Sandhoff disease, one of the GM2 gangliosidoses, is a lysosomal storage disorder characterized by the absence of beta-hexosaminidase A and B activity and the concomitant lysosomal accumulation of its substrate, GM2 ganglioside. It features catastrophic neurodegeneration and death in early childhood. How the lysosomal accumulation of ganglioside might affect the early development of the nervous system is not understood. Recently, cerebral organoids derived from induced pluripotent stem (iPS) cells have illuminated early developmental events altered by disease processes. To develop an early neurodevelopmental model of Sandhoff disease, we first generated iPS cells from the fibroblasts of an infantile Sandhoff disease patient, then corrected one of the mutant HEXB alleles in those iPS cells using CRISPR/Cas9 genome-editing technology, thereby creating isogenic controls. Next, we used the parental Sandhoff disease iPS cells and isogenic HEXB-corrected iPS cell clones to generate cerebral organoids that modeled the first trimester of neurodevelopment. The Sandhoff disease organoids, but not the HEXB-corrected organoids, accumulated GM2 ganglioside and exhibited increased size and cellular proliferation compared with the HEXB-corrected organoids. Whole-transcriptome analysis demonstrated that development was impaired in the Sandhoff disease organoids, suggesting that alterations in neuronal differentiation may occur during early development in the GM2 gangliosidoses

Case Study in Urban Context O Nexus Água-Energia: Revisão de Literatura em Gestão de Sistemas Integrados, Desafios e Oportunidades. Caso de Estudo em Contexto Urbano

In a society where Energy and Water walk side by side in practically all sectors of activity, it becomes necessary to analyze them from a productive, economic and sustainable point of view. From the extraction of mines and wells, to its treatment and transportation, and to the cooling systems of energy production, and despite being independently developed, managed and regulated, it is known that water needs energy and energy needs water. All these concerns, decision-making and the relationship between water and energy by itself create an opportunity to improve management in both sectors. There is an urgent need to consider all the assumptions and alternatives to improve the system and reduce energy consumption, which includes technological modifications and optimized resources management, leading to a more sustainable future. The increasing demand for energy and its exponential trend will have repercussions at all levels and will consequently increase the strain on freshwater resources with the most affected agricultural and industrial sectors. By improving the efficiency of water and energy use, there are positive repercussions that allow for substantial savings, especially where there is a lack of such resources. This article aims to address all these crucial issues related to energy and water in order to increase knowledge on the subject and to help promote alternatives for managing and developing integrated management systems and, consequently, for a rational use of both resources and a more sustainable future.Numa sociedade onde a Energia e a Água caminham lado a lado em praticamente todos os setores de atividade, torna-se necessário analisá-los sob um ponto de vista produtivo, económico e sustentável. Desde a extração de minas e poços, até ao respetivo tratamento e transporte e ainda aos sistemas de arrefecimento da produção de energia, e apesar de independentemente desenvolvidos, geridos e regulados, é sabido que a água precisa de energia e a energia de água. Todas estas preocupações, tomadas de decisão e a própria relação entre a água e a energia, criam uma oportunidade para melhorar a gestão de ambos os setores. Existe uma necessidade urgente de considerar todas as hipóteses e alternativas para melhorar o sistema e reduzir o consumo energético, que incluem algumas modificações tecnológicas e uma gestão otimizada dos recursos, levando a um futuro mais sustentável. A crescente procura de energia e a sua tendência exponencial trará repercussões a todos os níveis e consequentemente aumentará a tensão sobre os recursos de água doce, com os setores agrícola e industrial mais afetados. Ao melhorar a eficiência do uso da água e energia, surgem repercussões muito positivas que permitem uma poupança substancial, especialmente onde existe falta de tais recursos. O presente artigo pretende abordar todas estas questões cruciais relacionadas com a energia e água, a fim de aumentar o conhecimento sobre a matéria e ajudar a promover alternativas para gerir e desenvolver sistemas de gestão integrados e, consequentemente, encaminhar para um uso racional de ambos os recursos e um futuro mais sustentável.info:eu-repo/semantics/publishedVersio

Feasibility of a Monitoring Mechanism Supporting a Watch List under the Water Framework Directive

This report describes work conducted by the European Commission’s Joint Research Centre in the context of its support to the implementation of the Water Framework Directive 2000/60/EC. The work aimed at the feasibility assessment of an experimental monitoring exercise in support to a so-called Watch List Mechanism in a collaborative design involving EU Member States laboratories and some 200 official monitoring station operated by the Member States. The report includes all details on sampling stations, performance of analytical methods as well as the results of the analyses of all samples with regard to the occurrence and levels of 20 compounds of concern. In total, 219 whole water samples originating from 25 EU Member States and 2 other European countries, were assessed for contents of acesulfame, glyphosate and its metabolite AMPA, 1H-Benzotriazole and tolyltriazoles, bisphenol A, triclosan and triclocarban, carbamazepine and its metabolite 10,11-dihydro-10,11-dihydroxycarbamazepine, sulfamethoxazole, perfluoropropionic acid, tris-2-chloropropyl phosphate, methyl tert-butyl ether, silver, boron and chloride (Cl-) in water. Furthermore, 23 sediment samples were analysed for decabromodiphenylethane and decabromodiphenyl ether. The underlying analytical methods are carefully documented with regards to their performance characteristics. Obtained results are assessed statistically and where possible compared to other findings. Although the analysed single samples are insufficient to make any statement on the performance of the treatment processes leading to the compost, the collective of data allows having a glance at the pan-European situation as regards the studies compounds. Background information from literature describing the situation before the survey is included, too. The report is divided into a core part and two annexes. For practical reasons, the report is split into two volumes: Volume 1 contains the report and the single analytical results; volume 2 contains the documentation of the sampling stations.JRC.H.1-Water Resource

Trabalho, terra e geração de renda em três décadas de reflorestamentos no alto Jequitinhonha.

A partir dos anos 1970, as chapadas do Alto Jequitinhonha, localizadas no nordeste de Minas Gerais, até então áreas de uso comum de agricultores familiares, foram plantadas com eucaliptos. O objetivo deste artigo é comparar os efeitos do reflorestamento sobre a estrutura fundiária, valor da produção agrícola e ocupação rural com os efeitos da produção agrícola familiar sobre essas mesmas variáveis na microrregião homogênea de Capelinha, no Alto Jequitinhonha. Tal microrregião foi escolhida para o estudo por ser a área de maior concentração de eucaliptais da região. Foram utilizados dados secundários dos Censos do IBGE para os anos de 1970, 1980, 1985 e 1996, além de dados secundários de pesquisas realizadas sobre a região em questão, incluindo entrevistas com dirigentes de empresas e lideranças sindicais. O artigo conclui que, em trinta anos, o reflorestamento concentrou terras e criou um número reduzido de empregos; a agricultura familiar, ao contrário, teve suas áreas de terras comprimidas e super-exploradas em decorrência da perda das chapadas, mas continuou sendo a principal responsável pela geração de ocupações e rendas na região

New endoperoxides highly active in vivo and in vitro against artemisinin-resistant Plasmodium falciparum

Background: The emergence and spread of Plasmodium falciparum resistance to artemisinin-based combination therapy in Southeast Asia prompted the need to develop new endoperoxide-type drugs. Methods: A chemically diverse library of endoperoxides was designed and synthesized. The compounds were screened for in vitro and in vivo anti-malarial activity using, respectively, the SYBR Green I assay and a mouse model. Ring survival and mature stage survival assays were performed against artemisinin-resistant and artemisinin-sensitive P. falciparum strains. Cytotoxicity was evaluated against mammalian cell lines V79 and HepG2, using the MTT assay. Results: The synthesis and anti-malarial activity of 21 new endoperoxide-derived compounds is reported, where the peroxide pharmacophore is part of a trioxolane (ozonide) or a tetraoxane moiety, flanked by adamantane and a substituted cyclohexyl ring. Eight compounds exhibited sub-micromolar anti-malarial activity (IC50 0.3–71.1 nM), no cross-resistance with artemisinin or quinolone derivatives and negligible cytotoxicity towards mammalian cells. From these, six produced ring stage survival < 1% against the resistant strain IPC5202 and three of them totally suppressed Plasmodium berghei parasitaemia in mice after oral administration. Conclusion: The investigated, trioxolane–tetrazole conjugates LC131 and LC136 emerged as potential anti-malarial candidates; they show negligible toxicity towards mammalian cells, ability to kill intra-erythrocytic asexual stages of artemisinin-resistant P. falciparum and capacity to totally suppress P. berghei parasitaemia in mice.info:eu-repo/semantics/publishedVersio