2,511 research outputs found
Algumas espécies coprófilas de Psilocybe (Strophariaceae) do Estado de Pernambuco, Brasil
Coprophilous species Psilocybe argentina, P. pegleriana and P. cubensis are reported for the first time for Northeast Brazil. The last one is a hallucinogenic mushroom. Descriptions, discussions and drawings of the species are provided.As espĂ©cies coprĂłfilas Psilocybe argentina, P. pegleriana e P. cubensis sĂŁo referidas pela primeira vez para o Nordeste Brasileiro, sendo esta Ășltima uma espĂ©cie de cogumelo alucinĂłgeno. DescriçÔes, discussĂ”es e desenhos das espĂ©cies sĂŁo fornecidos
The Impact Of Cardiac Diseases During Pregnancy On Severe Maternal Morbidity And Mortality In Brazil.
To evaluate maternal heart disease as a cause or complicating factor for severe morbidity in the setting of the Brazilian Network for Surveillance of Severe Maternal Morbidity. Secondary data analysis of this multicenter cross-sectional study was implemented in 27 referral obstetric units in Brazil. From July 2009 to June 2010, a prospective surveillance was conducted among all delivery hospitalizations to identify cases of severe maternal morbidity (SMM), including Potentially Life-Threatening Conditions (PLTC) and Maternal Near Miss (MNM), using the new criteria established by the WHO. The variables studied included: sociodemographic characteristics, clinical and obstetric history of the women; perinatal outcome and the occurrence of maternal outcomes (PLTC, MNM, MD) between groups of cardiac and non-cardiac patients. Only heart conditions with hemodynamic impact characterizing severity of maternal morbidity were considered. 9555 women were included in the Network with severe pregnancy-related complications: 770 maternal near miss cases and 140 maternal death cases. A total of 293 (3.6%) cases were related to heart disease and the condition was known before pregnancy in 82.6% of cases. Maternal near miss occurred in 15% of cardiac disease patients (most due to clinical-surgical causes, p<0.001) and 7.7% of non-cardiac patients (hemorrhagic and hypertensive causes, p<0.001). Maternal death occurred in 4.8% of cardiac patients and in 1.2% of non-cardiac patients, respectively. In this study, heart disease was significantly associated with a higher occurrence of severe maternal outcomes, including maternal death and maternal near miss, among women presenting with any severe maternal morbidity.10e014438
Worrying news for brazilian caatinga : prevalence of batrachochytrium dendrobatidis in amphibians
The chytrid fungus Batrachochytrium dendrobatidis (Bd) has been linked to the declines of more than 500 amphibian species globally. In Brazil, Bd has been identified in several regions but predominantly in the Atlantic Forest. Data on the occurrence of this amphibian pathogen in northeastern Brazil are scarce, specifically from the Caatinga ecoregion, where there is only one study that reports Bd in the region. This study is the first to show a high prevalence of Bd in Caatingaâs species and includes two new records of species infected by Bd: Rhinella granulosa and R. jimi. In addition, we discuss the possibility of amphibian with terrestrial habits serving as potential Bd reservoirs in semiarid climate regions12CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTĂFICO E TECNOLĂGICO - CNPQFUNDAĂĂO DE AMPARO Ă PESQUISA DO ESTADO DE SĂO PAULO - FAPESP155556/2018â52015/11821â0; 2016/ 25358â3The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Financial support was provided by grants from CoordenacĂŁo de Aperfeiçoamento de Pessoal de N ıvel Superior, Conselho Nacional de Desenvolvimento Cient ıfico e Tecnologico (#155556/2018â5), and FundacĂŁo de Amparo a` Pesquisa do Estado de S~ao Paulo (#2015/11821â0; #2016/ 25358â3
Promoter hypermethylation of cancer-related genes: a strong independent prognostic factor in acute lymphoblastic leukemia
Promoter hypermethylation plays an important
role in the inactivation of cancerrelated
genes. This abnormality occurs
early in leukemogenesis and seems to be
associated with poor prognosis in acute
lymphoblastic leukemia (ALL). To determine
the extent of hypermethylation in
ALL, we analyzed the methylation status
of the CDH1, p73, p16, p15, p57, NES-1,
DKK-3, CDH13, p14, TMS-1, APAF-1,
DAPK, PARKIN, LATS-1, and PTEN genes
in 251 consecutive ALL patients.Atotal of
77.3% of samples had at least 1 gene
methylated, whereas 35.9% of cases had
4 or more genes methylated. Clinical features
and complete remission rate did not
differ among patients without methylated
genes, patients with 1 to 3 methylated
genes (methylated group A), or patients
with more than 3 methylated genes (methylated
group B). Estimated disease-free
survival (DFS) and overall survival (OS) at
11 years were 75.5% and 66.1%, respectively,
for the nonmethylated group; 37.2%
and 45.5% for methylated group A; and
9.4% and 7.8% for methylated group B
(P < .0001 and P .0004, respectively).
Multivariate analysis demonstrated that
the methylation profile was an independent
prognostic factor in predicting DFS
(P < .0001) and OS (P .003). Our results
suggest that the methylation profile may
be a potential new biomarker of risk prediction
in AL
In vivo comparative study of the effects of using the enamel matrix derivative and/or photobiomodulation on the repair of bone defects
The repair of bone defects has been the subject of many studies that have shown inconclusive results as to what is the best bone substitute. Bone defects (Ă 2 mm) were induced on the tibia of seventy-two rats, which were distributed into the following
Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies
To become fertile, mammalian sperm must undergo a series of biochemical and physiological changes known as capacitation. These changes involve crosstalk between metabolic and signaling pathways and can be recapitulated in vitro. In this work, sperm were incubated in the absence of exogenous nutrients (starved) until they were no longer able to move. Once immotile, energy substrates were added back to the media and sperm motility was rescued. Following rescue, a significantly higher percentage of starved sperm attained hyperactivated motility and displayed increased ability to fertilize in vitro when compared with sperm persistently incubated in standard capacitation media. Remarkably, the effects of this treatment continue beyond fertilization as starved and rescued sperm promoted higher rates of embryo development, and once transferred to pseudo-pregnant females, blastocysts derived from treated sperm produced significantly more pups. In addition, the starvation and rescue protocol increased fertilization and embryo development rates in sperm from a severely subfertile mouse model, and when combined with temporal increase in Ca2+ ion levels, this methodology significantly improved fertilization and embryo development rates in sperm of sterile CatSper1 KO mice model. Intracytoplasmic sperm injection (ICSI) does not work in the agriculturally relevant bovine system. Here, we show that transient nutrient starvation of bovine sperm significantly enhanced ICSI success in this species. These data reveal that the conditions under which sperm are treated impact postfertilization development and suggest that this âstarvation and rescue methodâ can be used to improve assisted reproductive technologies (ARTs) in other mammalian species, including humans.Fil: Navarrete, Felipe A.. University of Massachussets; Estados UnidosFil: Aguila, Luis. University of Massachussets; Estados UnidosFil: Martin Hidalgo, David. University of Massachussets; Estados Unidos. Universidad de Extremadura ; EspañaFil: Tourzani, Darya A.. University of Massachussets; Estados UnidosFil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de BiologĂa y Medicina Experimental. FundaciĂłn de Instituto de BiologĂa y Medicina Experimental. Instituto de BiologĂa y Medicina Experimental; ArgentinaFil: Ardestani, Goli. University of Massachussets; Estados UnidosFil: Garcia Vazquez, Francisco A.. Universidad de Murcia; EspañaFil: Levin, Lonny R.. Cornell University; Estados UnidosFil: Buck, Jochen. Cornell University; Estados UnidosFil: Darszon, Alberto. Universidad Nacional AutĂłnoma de MĂ©xico. Instituto de BiologĂa; MĂ©xicoFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de BiologĂa y Medicina Experimental. FundaciĂłn de Instituto de BiologĂa y Medicina Experimental. Instituto de BiologĂa y Medicina Experimental; ArgentinaFil: Mager, Jesse. University of Massachussets; Estados UnidosFil: Fissore, Rafael A.. University of Massachussets; Estados UnidosFil: Salicioni, Ana M.. University of Massachussets; Estados UnidosFil: Gervasi, MarĂa G.. University of Massachussets; Estados UnidosFil: Visconti, Pablo E.. University of Massachussets; Estados Unido
Down-Regulation of hsa-miR-10a in Chronic Myeloid Leukemia CD34+ Cells Increases USF2-Mediated Cell Growth
MicroRNAs (miRNA) are small noncoding,
single-stranded RNAs that inhibit gene expression at a
posttranscriptional level, whose abnormal expression
has been described in different tumors. The aim of our
study was to identify miRNAs potentially implicated
in chronic myeloid leukemia (CML). We detected an
abnormal miRNA expression profile in mononuclear and
CD34+ cells from patients with CML compared with
healthy controls. Of 157 miRNAs tested, hsa-miR-10a,
hsa-miR-150, and hsa-miR-151 were down-regulated,
whereas hsa-miR-96 was up-regulated in CML cells.
Down-regulation of hsa-miR-10a was not dependent
on BCR-ABL1 activity and contributed to the increased
cell growth of CML cells. We identified the upstream
stimulatory factor 2 (USF2) as a potential target of
hsa-miR-10a and showed that overexpression of USF2
also increases cell growth. The clinical relevance of
these findings was shown in a group of 85 newly
diagnosed patients with CML in which expression of
hsa-miR-10a was down-regulated in 71% of the patients,
whereas expression of USF2 was up-regulated in 60% of
the CML patients, with overexpression of USF2 being
significantly associated with decreased expression of
hsa-miR-10a (P = 0.004). Our results indicate that
down-regulation of hsa-miR-10a may increase USF2 and
contribute to the increase in cell proliferation of CML
implicating a miRNA in the abnormal behavior of CML
It Takes Two to Tango, Part II : Synthesis of A-Ring Functionalised Quinones Containing Two Redox-Active Centres with Antitumour Activities
In 2021, our research group published the prominent anticancer activity achieved through
the successful combination of two redox centres (ortho-quinone/para-quinone or quinone/seleniumcontaining triazole) through a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. The
combination of two naphthoquinoidal substrates towards a synergetic product was indicated, but
not fully explored. Herein, we report the synthesis of 15 new quinone-based derivatives prepared
from click chemistry reactions and their subsequent evaluation against nine cancer cell lines and
the murine fibroblast line L929. Our strategy was based on the modification of the A-ring of paranaphthoquinones and subsequent conjugation with different ortho-quinoidal moieties. As anticipated,
our study identified several compounds with IC50 values below 0.5 ”M in tumour cell lines. Some
of the compounds described here also exhibited an excellent selectivity index and low cytotoxicity
on L929, the control cell line. The antitumour evaluation of the compounds separately and in their
conjugated form proved that the activity is strongly enhanced in the derivatives containing two
redox centres. Thus, our study confirms the efficiency of using A-ring functionalized para-quinones
coupled with ortho-quinones to obtain a diverse range of two redox centre compounds with potential
applications against cancer cell lines. Here as well, it literally takes two for an efficient tango
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