Alcanzar por medio de la comunicación, actitudes y comportamientos positivos en las relaciones entre los niños(as), familiares y laborales en el contexto del hogar infantil centro social San Vicente la Dorada- Caldas.

El hogar infantil centro social san Vicente es una Institución de protección al menor, de utilidad común de derecho privado, sin ánimo de lucro, vinculado al sistema nacional de Bienestar Familiar de conformidad con lo preceptuado por la ley 7 de 1979 y su decreto reglamentarios 2388 del mismo año, por lo tanto sujetado a las normas que lo rigen; teniendo en cuenta las disposiciones que sobre la materia emane el gobierno nacional. El hogar infantil centro social san Vicente contribuye al fortalecimiento y convivencia familiar, social e institucional a través de la promoción y prevención, generando diferentes estrategias en pro de la inclusión activa de la familia e institución como en el desarrollo integral en los aspectos bilógicos, cognitivos, psicomotriz, socio- afectivos y espiritual; prevaleciendo el reconocimiento a la igualdad y la dignidad humana, sin discriminación alguna. Es así como nace la necesidad de generar procesos e intervenciones para unificar y trabajar el vínculo Psicoafectivo desde lo individual, grupal y familiar en los diferentes contextos. Se propuso desde la Universidad a través de la experiencia profesional dirigida, y de acuerdo a un diagnóstico inicial realizado el segundo semestre del año 2012, el cual muestra que los niños y niñas del Hogar Infantil, familias y grupo de empleados , en su mayoría exteriorizan la inadecuada actitud, comportamiento en el momento de relacionarse, el irrespeto asía el otro, actos y hábitos agresivos al interior del grupo de compañeros, niños(as) y familia, llevando a experimentar una incomunicación total y apatía en los diferentes contexto. Lo anterior afirma el interés y la calidad en la búsqueda de herramientas necesarias para una atención y orientación donde el amor, interés y sentido de pertenencia prevalezcan; para lograr interiorizar el desarrollo socio-afectivo; ya que el niño juega un papel fundamental en el afianzamiento de su personalidad, siendo esencial para su fortalecimiento, en las relaciones que establece con los padres, hermanos, docentes, niños, niñas y adultos cercanos; lo cual consiste en orientar su vida, es decir, llevar al niño poco a poco a relacionarse con su entorno, sus semejantes, y por otro lado, permitir apreciaciones sobre la sociedad y su papel en ella, durante el tiempo de atención y orientación; el objetivo es trabajar en equipo con el núcleo familiar, considerando que desde allí se puede dar respuesta a muchas de los problemas de conductas en beneficio de los niños y niñas en donde fomentar y fortalecer sus relaciones familiares es de gran ayuda para prevenir actitudes y comportamientos impropios. Como alternativa principal de intervención se busca generar un mejoramiento en la dinámica familiar considerando importante el transformar desde el núcleo familiar, aquellas pautas de comportamientos y actitudes que generan inestabilidad emocional en los niños y niñas, familia y empleados del Hogar Infantil Centro Social San Vicente.The children's home St. Vincent's social center is a child protection institution, common utility private, non-profit, linked to the National Family Welfare System in accordance with the provisions of the law in July 1979 and its regulatory Decree 2388 the same year, therefore subjected to the rules that govern it, taking into account the provisions on the subject adopted the national government. The children's home St. Vincent community center and contributes to strengthening family life, social and institutional development through promotion and prevention, generating different strategies towards active inclusion of the family and institution as integral development bilógicos aspects, cognitive , psychomotor, socio-affective and spiritual prevailing recognition of equality and human dignity without discrimination. Thus was born the need to create processes and interventions to unify and work the link psycho from the individual, group and family in different contexts. It was proposed by the University through targeted professional experience, and according to an initial diagnosis made the second half of 2012, which shows that children Children's Home, families and group of employees, mostly externalized inadequate attitude, behavior at the time of relating, disrespect grabbing the other aggressive acts and habits within the peer group, children (as) and family, leading to experience total isolation and apathy in different context. This affirms the interest and quality in the search for tools to care and guidance where love, interest and ownership prevail, to achieve internalize socio-emotional development, as the child plays a key role in strengthening of his personality, being essential for strengthening established relationships with parents, siblings, teachers, children and adults around, which is to guide your life, that is, take the child gradually relate their environment, peers, and on the other hand, allow insights into society and their role in it, during the time of care and guidance, the aim is to work together with the family, considering that from there you can answer many of the problems of conduct for the benefit of children in which to promote and strengthen family relationships is helpful to prevent improper attitudes and behaviors. Alternatively main intervention is to generate an improvement in family dynamics important considering the transformation from the family, those patterns of behaviors and attitudes that cause emotional instability in children, family and employees of the Children's Home St. Vincent Community Centre

2-hydroxyethilammonium oleate protic ionic liquid as corrosion inhibitor for aluminum in neutral medium

Protic ionic liquid (PIL) 2‐hydroxyethylammonium oleate (2HEAOl) proved to be a good lubricant for aluminum‐forming processes. However, with the aim of keeping the formed component integrity, it is interesting that the same substance employed during forming does not need to be removed and works out as corrosion inhibitor. Then, the aim of this study was to test the performance of 2HEAOl as corrosion inhibitor for aluminum in neutral 0.5 mol/L NaCl medium by electrochemical characterization. Results showed that the concentration of 5 × 10−4 mol/L was a suitable concentration to promote corrosion inhibition until 72 h at the high chloride concentration studied. The PIL worked out as mixed‐type organic corrosion inhibitor, as it promoted the diminution of the oxygen reduction reaction rate and, in consequence, the pit initiation by its adsorption on the metal surface

The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm

Sperm capacitation is required for fertilization. At the molecular level, this process is associated with fast activation of protein kinase A. Downstream of this event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity of the tyrosine kinase(s) mediating this process has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested a role for PYK2 based on the use of small molecule inhibitors directed against this kinase. However, crucially, loss-of-function experiments have not been reported. Here, we used both pharmacological inhibitors and genetically modified mice models to investigate the identity of the tyrosine kinase(s) mediating the increase in tyrosine phosphorylation in mouse sperm. Similar to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphorylation. Yet, sperm from Pyk2(-/-) mice displayed a normal increase in tyrosine phosphorylation, implying that PYK2 is not responsible for this phosphorylation process. Here, we show that PF431396 can also inhibit FER, a tyrosine kinase known to be present in sperm. Sperm from mice targeted with a kinase-inactivating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphorylation. Although these mice are fertile, their sperm displayed a reduced ability to fertilize metaphase II-arrested eggs in vitro.Fil: Alvau, Antonio. University of Massachussets; Estados UnidosFil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gervasi, Maria Gracia. University of Massachussets; Estados UnidosFil: Navarrete, Felipe A.. University of Massachussets; Estados UnidosFil: Xu, Xinran. State University of Colorado - Fort Collins; Estados UnidosFil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: De la Vega Beltran, José Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Greer, Peter. Queens University; CanadáFil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Krapf, Diego. State University of Colorado - Fort Collins; Estados UnidosFil: Salicioni, Ana María. University of Massachussets; Estados UnidosFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Visconti, Pablo E.. University of Massachussets; Estados Unido

Genomic variability in Mexican chicken population using Copy Number Variation

Copy number variants (CNVs) are polymorphisms which influence phenotypic variation and are an important source of genetic variability [1]. In Mexico the backyard poultry population is a unique widespread Creole chicken (Gallus gallus domesticus) population, an undefined cross among different breeds brought to Mexico from Europe and under natural selection for almost 500 years [2-3]. The aim of this study was to investigate genomic variation in the Mexican chicken population using CNVs.A total of 256 DNA samples genotyped with Axiom® Genome-Wide Chicken Genotyping Array were used in the analyses. The individual CNV calling, based on log-R ratio and B-allele frequency values, was performed using the Hidden Markov Model (HMM) of PennCNV software on the autosomes [4-5]. CNVs were summarized to CNV regions (CNVRs) at a population level (i.e. overlapping CNVs), using BEDTools.The HMM detected a total of 1924 CNVs in the genome of 256 samples resulting, at population level, in 1216 CNV regions, of which 959 gains, 226 losses and 31 complex CNVRs (i.e. containing both losses and gains), covering a total of 47 Mb of sequence length corresponding to 5,12 % of the chicken galGal4 assembly autosome. A comparison among this study and 7 previous reports about CNVs in chicken was performed, finding that the 1,216 CNVRs detected in this study overlap with 617 regions (51%) mapped by others studies. This study allowed a deep insight into the structural variation in the genome of unselected Mexican chicken population, which up to now has not been never genetically characterized with SNP markers. Based on a cluster analysis (pvclust – R package) on CNV markers the population, even if presenting extreme morphological variation, does not resulted divided in differentiated genetic subpopulations. Finally this study provides a CNV map based on the 600K SNP chip array jointly with a genome-wide gene copy number estimates in Mexican chicken population.

An amino-based protic ionic liquid as a corrosion inhibitor of mild steel in aqueous chloride solutions

Protic ionic liquids (PILs) have shown to be promising substances as corrosion inhibitors (CIs). In line with this, the aim of this study is to study the performance and propose the corrosion inhibition mechanism of N‐methyl‐2‐ hydroxyethylamine (M‐2HEAOL) and bis‐2‐hidroxyethylamine (B‐HEAOL) oleate, for mild steel, in a neutral chloride solution. Electrochemical characterization was conducted under static and hydrodynamic conditions, and it was revealed that M‐2HEAOL and B‐HEAOL worked as mixed‐type CIs with more interference on the anodic reaction. Inhibition efficiency depended on the concentration reaching 97% of inhibition efficiency in 5 mmol/L concentration. Scanning electron microscopy, optical interferometry, Raman spectroscopy, and Fourier‐transform infrared spectroscopy are used to elicit the chemical composition of the surface film and corrosion morphology of steel in the presence of CIs, the adsorption processes of which involved physical and chemical adsorption between metal and different parts of ionic liquids. The results allowed the proposition of a corrosion inhibition mechanism

N-methyl-2-hydroxyethylammonium oleate ionic liquid performance as corrosion inhibitor for mild steel in hydrochloric acid medium

The aim of the present study is to evaluate the performance of N‐methyl‐2‐ hydroxyethylammonium oleate ([m‐2HEA][Ol]) as a corrosion inhibitor for mild steel in a 0.1‐mol/L hydrochloric acid solution and also investigate the role of chloride in the inhibition mechanism. This protic ionic liquid (PIL) has formerly shown a high efficiency as a corrosion inhibitor in a neutral chloride medium. Electrochemical and weight loss measurements, surface contact angle determination, scanning electron microscopy, and Raman spectroscopy were used to understand the factors that influence the response of the studied inhibitor. Results revealed that [m‐2HEA][Ol] behaves as a mixed‐type adsorption inhibitor, by blocking cathodic sites and by modifying the activation energy of the anodic reaction, and it can reach up to 94–97% of inhibition efficiency. PIL adsorption was enhanced by the excess of positive charge of the mild steel. The effect of inhibitor molecule has been discussed to propose a mechanism that explains the inhibitory action of the corrosion inhibitor, pointing out the role of chloride in the inhibition mechanism

Baseline data of four insecticides with different modes of action for Anastrepha fraterculus and Ceratitis capitata (Diptera: Tephritidae)

La mosca de la fruta del Mediterráneo, Ceratitis capitata (Wiedemann), y la mosca sudamericana de los frutos, Anastrepha fraterculus (Wiedemann) son plagas de gran importancia económica en Argentina y el mundo. Una de las formas de control es el uso de insecticidas en pulverizaciones totales o en cebos. El uso reiterado de un determinado producto ejerce una fuerte presión de selección, pudiendo ocasionar la aparición de resistencia. El objetivo de este trabajo fue determinar el dato de nivel básico de cuatro insecticidas con diferentes mecanismos de acción mediante bioensayos en laboratorio. Los productos utilizados fueron formulaciones comerciales de ciantraniliprol, clorpirifos, lambdacialotrina y spinosad. La concentración letal media se estimó mediante un análisis de la curva dosis-respuesta. Para ciantraniliprol y clorpirifós los machos y las hembras de ambas especies fueron igualmente susceptibles. Para lambdacialotrina se detectaron diferencias entre las dos especies; la CL50 de A. fraterculus (0,058 y 0,074 para machos y hembras respectivamente) fue menor que la de C. capitata (0,523 y 0,624 para machos y hembras respectivamente). Para spinosad, los machos de A. fraterculus fueron igualmente susceptibles que los machos y las hembras de C. capitata. Estos resultados generan valores de referencia para Argentina y evidencian la necesidad de completar este tipo de estudios con evaluaciones de poblaciones naturales sobre las cuales se ejerce control químico así como con ensayos en campo y semi-campo. Asimismo muestran la necesidad de completar este tipo de estudios con evaluaciones de los niveles de ingesta en las distintas concentraciones.The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and the South American fruit fly, Anastrepha fraterculus (Wiedemann) are pests of major economic importance in Argentina and worldwide. One way to control them is the use of insecticide in sprays or baits. The repeated use of a particular product exerts a strong selection pressure and can lead to development of resistance. The aim of this study was to determine baseline data of four insecticides with different mechanisms of action through laboratory bioassays. The products used were commercial formulations of cyantraniliprole, chlorpyrifos, lambdacyalothrin and spinosad. The mean lethal concentration was estimated by a dose-response curve analysis. Cyantraniliprole and chlorpyrifos were equally susceptible for males and females of both species. For lambdacyalothrin differences between the two species were detected; LC50 of A. fraterculus (0.058 and 0.074 for males and females respectively) was lower than that of C. capitata (0.523 and 0.624 for males and females respectively). For spinosad, A. fraterculus males were equally susceptible than males and females of C. capitata. These results generate reference values for Argentina and demonstrate the need to complete these studies with field evaluations of natural populations on which chemical control is exercised as well as with field and semi-field trials. It also shows the need to complete these studies in which the intake at different concentrations is measured.Fil: Paez Jerez, P. G.. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Cátedra. Terapéutica Vegetal; ArgentinaFil: Carrizo, B. N.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Musse, R.. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Cátedra. Terapéutica Vegetal; ArgentinaFil: Varela, E.. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Cátedra. Terapéutica Vegetal; ArgentinaFil: Armiñana, A.. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Cátedra. Terapéutica Vegetal; ArgentinaFil: Milla, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Vera, Maria Teresa. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Cátedra. Terapéutica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de la Vega, M. H.. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Cátedra. Terapéutica Vegetal; Argentin

Morphological and molecular evidence of Oslerus osleri (Nematoda: Filaroididae) in the Andean fox (Lycalopex culpaeus)

Oslerus osleri is a cosmopolitan filaroid nematode that parasitizes the respiratory system of domestic and wild canids. Natural infection by O. osleri is reported in the Andean fox (Lycalopex culpaeus) in this study. Nematodes, enclosed in small and compact fibrous nodules of 1 to 5 mm in diameter, were found on the surface of the trachea near the bronchial bifurcation on four Andean foxes during necropsy (one from Cuzco, Peru and three from Northwestern Patagonia in Argentina). The nematodes were identified as O. osleri by morphological and molecular methods. Ribosomal and mitochondrial DNA analyses were performed amplifying the second internal transcribed spacer region (ITS-2), the partial cytochrome c oxidase 1 (cox1), and the large subunit of nuclear ribosomal RNA (LSU rRNA) genes. Sequences of the ITS-2 and LSU rRNA had a genetic variation of 1.5% and 1.0%, respectively, with previous sequences of O. osleri registered in Genbank. This is the first amplification of the cox1 gene of O. osleri and demonstrated an identity of 92% to Perostrongylus falciformis (KY365437), and 90% to Angiostrongylus cantonensis (KY779735) and Angiostrongylus costaricensis (AP017675).Fil: Gomez Puerta, Luis A.. Universidad Nacional Mayor de San Marcos; PerúFil: Flores, Verónica Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Departamento de Zoología. Laboratorio de Parasitología; ArgentinaFil: Vega, Rocío Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Departamento de Zoología. Laboratorio de Parasitología; ArgentinaFil: Brugni, Norma Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Departamento de Zoología. Laboratorio de Parasitología; ArgentinaFil: Viozzi, Gustavo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Departamento de Zoología. Laboratorio de Parasitología; ArgentinaFil: Lopez Urbina, Maria T.. Universidad Nacional Mayor de San Marcos; PerúFil: Gonzalez, Armando E.. Universidad Nacional Mayor de San Marcos; Per