The molecular chaperone Hsp90 is a component of the cap-binding complex and interacts with the translational repressor Cup during Drosophila oogenesis

In metazoa, the spatio-temporal translation of diverse mRNAs is essential to guarantee proper oocyte maturation and early embryogenesis. The eukaryotic translation initiation factor 4E (eIF4E), which binds the 5′ cap structure of eukaryotic mRNAs, associates with either stimulatory or inhibitory factors to modulate protein synthesis. In order to identify novel factors that might act at the translational level during Drosophila oogenesis, we have undertaken a functional proteomic approach and isolated the product of the Hsp83 gene, the evolutionarily conserved chaperone Hsp90, as a specific component of the cap-binding complex. Here we report that Hsp90 interacts in vitro with the translational repressor Cup. In addition, we show that Hsp83 and cup interact genetically, since lowering Hsp90 activity enhances the oogenesis alterations linked to diverse cup mutant alleles. Hsp90 and Cup co-localize in the cytoplasm of the developing germ-line cells within the germarium, thus suggesting a common function from the earliest stages of oogenesis. Taken together, our data start elucidating the role of Hsp90 during Drosophila female germ-line development and strengthen the idea that Cup has multiple essential functions during egg chamber development

Familiares visitantes e acompanhantes de adultos e idosos hospitalizados: análise da experiência na perspectiva do processo de trabalho em enfermagem

This is a qualitative study using Grounded Theory as the methodological reference and the Process of Work in Nursing as the theoretical reference in order to understand the role assumed by nurses regarding hospital norms and routines applied to family visitors and companions of adult and elderly patients in a teaching hospital. Data analysis identified the theme: defining the modality of family support during hospitalization. This theme aggregates two main categories: becoming the family visitor and becoming the family companion. Through the analysis, it could be observed how established rules, which aims at disciplining and developing an efficient work in the hospital, can expose the lack of autonomy in the work process to modify relations in this context and how the familiar appropriation, as part of the health team, is far from being considered in the institutions.Se trata de un estudio cualitativo que utiliza como referencial metodológico la Grounded Theory y como referencial teórico el Proceso de Trabajo en Enfermería para comprender el rol asumido por el enfermero frente a las normas y rutinas hospitalarias, de los familiares visitantes y de los acompañantes de adultos y ancianos internados en un hospital universitario. El análisis de los datos permitió la identificación del tema: definiéndose la modalidad de apoyo familiar durante la hospitalización, que reúne dos categorías principales: tornándose un familiar visitante y tornándose un familiar acompañante. Por medio del análisis, se puede profundizar la comprensión acerca de las reglas establecidas que, con el objetivo de disciplinar y hacer eficiente el trabajo desarrollado en el hospital, permite explicar la ausencia de autonomía en el proceso de trabajo, para de esta forma, modificar las relaciones en este contexto y resaltar la integración a la institución del familiar como parte del equipo de salud.Trata-se de estudo qualitativo, utilizando-se como referencial metodológico a Grounded Theory e como referencial teórico o Processo de Trabalho em Enfermagem, para compreender o papel assumido pelo enfermeiro perante as normas e rotinas hospitalares, relativas aos familiares visitantes e acompanhantes de adultos e idosos internados em um Hospital Universitário. A análise dos dados permitiu a identificação do tema: definindo-se a modalidade de apoio familiar durante a hospitalização, que reúne duas categorias principais: tornando-se familiar visitante e tornando-se familiar acompanhante. Por meio da análise, pôde-se aprofundar a compreensão do quanto as regras estabelecidas, com o objetivo de disciplinar e tornar eficiente o trabalho desenvolvido no hospital, podem explicitar o desprovimento de autonomia no processo de trabalho, para modificar as relações nesse contexto e o quanto a apropriação do familiar como parte da equipe de saúde, está distante de ser pensada no concreto das instituições

The role of single nucleotide polymorphisms related to iron homeostasis in mesothelioma susceptibility after asbestos exposure: a genetic study on autoptic samples

Asbestos-related diseases still represent a major public health problem all over the world. Among them, malignant mesothelioma (MM) is a poor-prognosis cancer, arising from the serosal lining of the pleura, pericardium and peritoneum, triggered by asbestos exposure. Literature data suggest the key role of iron metabolism in the coating process leading to the formation of asbestos bodies, considered to be both protective and harmful. Two sample sets of individuals were taken into consideration, both residing in Broni or neighboring cities (Northwestern Italy) where an asbestos cement factory was active between 1932 and 1993. The present study aims to compare the frequency of six SNPs involved in iron trafficking, previously found to be related to protection/predisposition to MM after asbestos exposure, between 48 male subjects with documented asbestos exposure who died of MM and 48 male subjects who were exposed to asbestos but did not develop MM or other neoplastic respiratory diseases (Non-Mesothelioma Asbestos Exposed - NMAE). The same analysis was performed on 76 healthy male controls. The allelic and genotypic frequencies of a sub-group of 107 healthy Italian individuals contained in the 1000 genomes database were considered for comparison. PCR-multiplex amplification followed by SNaPshot mini-sequencing reaction was used. The findings presented in this study show that the allelic and genotypic frequencies for six SNP markers involved in iron metabolism/homeostasis and the modulation of tumor microenvironment are not significantly different between the two sample sets of MM and NMAE. Therefore, the SNPs here considered do not seem to be useful markers for individual susceptibility to mesothelioma. This finding is not in agreement with previous literature

Perfil, evolução e desfecho dos pacientes atendidos pelo serviço de atendimento móvel de urgência

Objective: to analyze the profile, the evolution and outcome of patients served by the SAMU 192. Method: cross-sectional, exploratory and descriptive study. The sample consisted of data from 600 adult patients, served in the year 2015 by the SAMU and referred to another health service. Descriptive analysis was performed to identify the population profile, the evolution and the intra-hospital outcome; correlation testing between response time in the pre-hospital service and length of stay; and multiple logistic regression between response time and outcome. It used p < 0.05 as the significance level. Results: there was a predominance of care for clinical occurrences, male gender and average age of 55.5 years. After pre-hospital care, 50.2% of the surveyed patients remained under observation and 34.8% required hospitalization. The pre-hospital response time was positively correlated with the length of stay. The main outcome was medical discharge, with 41% of them being referred for the continuity of treatment. Conclusion: the analysis showed a profile of care provided to patients with an average age of 55 years and suffering from clinical diseases that required referral to a health unit, remaining under hospital observation for up to 12 hours. These results are important for the support of care flow protocols in the RUE, in order to reduce the overload of tertiary services.Objetivo: analisar perfil, evolução e desfecho dos pacientes atendidos pelo SAMU 192. Método: estudo transversal, exploratório e descritivo. A amostra constou dados de 600 pacientes adultos, atendidos no ano de 2015 pelo SAMU e encaminhados para outro serviço de saúde. Realizou-se análise descritiva para identificar o perfil da população, a evolução e o desfecho intra-hospitalar; teste de correlação entre o tempo resposta no serviço pré-hospitalar e o tempo de internação; e regressão logística múltipla entre o tempo resposta e o desfecho. Utilizou-se p < 0,05 como nível de significância. Resultados: predominaram o atendimento de ocorrências clínicas, o sexo masculino e a média de idade de 55,5 anos. Após o atendimento pré-hospitalar, 50,2% dos pacientes permaneceram em observação e 34,8% precisaram de internação hospitalar. O tempo resposta pré-hospitalar apresentou correlação positiva com o tempo de internação. O principal desfecho foi a alta médica, sendo que 41% deles foram encaminhados para continuidade do tratamento. Conclusão: a análise mostrou um perfil de atendimentos a pacientes com idade média de 55 anos e acometidos por doenças de natureza clínica que necessitaram de encaminhamento a uma unidade de saúde, permanecendo em observação hospitalar por até 12h. Esses resultados são importantes para o embasamento de protocolos de fluxo assistencial na RUE, a fim de diminuir a sobrecarga dos serviços terciários.info:eu-repo/semantics/publishedVersio

O absenteísmo da equipe de enfermagem em unidade de pronto socorro de um hospital universitário

O absenteísmo ou ausentismo é o termo utilizado para caracterizar as ausências dos funcionários ao trabalho. Essas ausências são definidas em previstas e não previstas. Para este estudo especificamos as ausências previstas como folgas, feriados e férias e para as não previstas ficam as faltas, licenças medicas, nojo e outras licenças. O objetivo foi investigar o real numero de ausências dos técnicos e auxiliares de enfermagem de um Pronto Socorro de um Hospital Universitário do interior do estado de São Paulo para definir o Índice de Segurança Técnica-IST. Foi utilizada metodologia quantitativa descritiva, e a coleta de dados feita de janeiro de 2006 a junho de 2007, a partir de arquivos administrativos após autorização do serviço. Entre os principais achados encontramos um IST cuja média gira em torno de 51%. Concluímos que, embora próximo, está ligeiramente superior ao encontrado em outra realidade de Hospital Universitário. Entre as ausências não previstas, destacam-se as licenças médicas, que podem estar relacionadas à sobrecarga da equipe

Health impact of the emissions from a refinery: case-control study on the adult population living in two municipalities in Lomellina, Italy

Background: In the municipalities of Sannazzaro de’ Burgondi and Ferrer Erbognone (District of Lomellina, Pavia, Lombardy, Italy), an oil refinery is operating since 1963. In 2008, the company running the plant (eni S.p.A.) asked the competent bodies the permission for building a new facility (“EST”). The present work is aimed at evaluating the ante-operam health impacts of the existing facility refinery. Methods: A case-control study design was implemented. Cases were subjects admitted to hospital in 2002-2014 due to acute respiratory, cardiovascular or gastrointestinal conditions. Controls were selected among those who had not been hospitalised in that timespan. Cases and controls had to be alive at enrolment, aged 20-64 years, and were frequency-matched by age, gender and municipality. Data were extracted from the health insurance registry and from Hospital Discharge Records (ATS Pavia). Enrolled subjects were asked to complete a mailed survey. Environmental exposure was the fallout of refinery emissions (PM10) at participants’ homes, as predicted by an AERMOD model. Results: 541 respondents (125 cases, 416 controls) were included in the analyses. Response bias was excluded. Individual PM10 exposure was not significantly different between cases and controls, while it was significantly associated with municipality (being higher in Sannazzaro). The crude effect estimate of PM10 over case/control status indicated a not-significant excess of hospitalisation with the increase in PM10 exposure. Multivariate analyses confirmed those results. Conclusion: Findings indicate a possible excess of hospitalisation risk in most exposed people, but the effect is not statistically significant and may be affected by bias

Systematic literature review informing the 2018 update of the EULAR recommendation for the management of large vessel vasculitis : focus on giant cell arteritis

© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.Objectives: To analyse the current evidence for the management of large vessel vasculitis (LVV) to inform the 2018 update of the EULAR recommendations. Methods: Two systematic literature reviews (SLRs) dealing with diagnosis/monitoring and treatment strategies for LVV, respectively, were performed. Medline, Embase and Cochrane databases were searched from inception to 31 December 2017. Evidence on imaging was excluded as recently published in dedicated EULAR recommendations. This paper focuses on the data relevant to giant cell arteritis (GCA). Results: We identified 287 eligible articles (122 studies focused on diagnosis/monitoring, 165 on treatment). The implementation of a fast-track approach to diagnosis significantly lowers the risk of permanent visual loss compared with historical cohorts (level of evidence, LoE 2b). Reliable diagnostic or prognostic biomarkers for GCA are still not available (LoE 3b).The SLR confirms the efficacy of prompt initiation of glucocorticoids (GC). There is no high-quality evidence on the most appropriate starting dose, route of administration, tapering and duration of GC (LoE 4). Patients with GCA are at increased risk of dose-dependent GC-related adverse events (LoE 3b). The addition of methotrexate or tocilizumab reduces relapse rates and GC requirements (LoE 1b). There is no consistent evidence that initiating antiplatelet agents at diagnosis would prevent future ischaemic events (LoE 2a). There is little evidence to guide monitoring of patients with GCA. Conclusions: Results from two SLRs identified novel evidence on the management of GCA to guide the 2018 update of the EULAR recommendations on the management of LVV.info:eu-repo/semantics/publishedVersio