Leveraging Change: Increasing Access to Arts Education in Rural Areas

In 2015, Massachusetts College of Liberal Arts (MCLA) received funding in the first round of collective impact grants from the National Endowment for the Arts to launch the pilot initiative, Leveraging Change: Improving Access to Arts Education in Rural Areas. The authors conducted research which included a literature review and interviews with arts education leaders in rural areas. Using the research compiled through this process, a pilot convening was held in western Massachusetts' Berkshire County to activate ideas, stimulate the exchange of information, and generate cross-sector collaboration focused on strengthening support for arts education in the region. This working paper is a summary of the research results and insights gleaned from this pilot initiative

New species of Styracosaurus

12 p. : ill. ; 24 cm.Includes bibliographical references (p. 12)

Human antibodies neutralizing diphtheria toxin in vitro and in vivo

Diphtheria is an infectious disease caused by Corynebacterium diphtheriae. The bacterium primarily infects the throat and upper airways and the produced diphtheria toxin (DT), which binds to the elongation factor 2 and blocks protein synthesis, can spread through the bloodstream and affect organs, such as the heart and kidneys. For more than 125 years, the therapy against diphtheria has been based on polyclonal horse sera directed against DT (diphtheria antitoxin; DAT). Animal sera have many disadvantages including serum sickness, batch-to-batch variation in quality and the use of animals for production. In this work, 400 human recombinant antibodies were generated against DT from two different phage display panning strategies using a human immune library. A panning in microtiter plates resulted in 22 unique in vitro neutralizing antibodies and a panning in solution combined with a functional neutralization screening resulted in 268 in vitro neutralizing antibodies. 61 unique antibodies were further characterized as scFv-Fc with 35 produced as fully human IgG1. The best in vitro neutralizing antibody showed an estimated relative potency of 454 IU/mg and minimal effective dose 50% (MED50%) of 3.0 pM at a constant amount of DT (4x minimal cytopathic dose) in the IgG format. The targeted domains of the 35 antibodies were analyzed by immunoblot and by epitope mapping using phage display. All three DT domains (enzymatic domain, translocation domain and receptor binding domain) are targets for neutralizing antibodies. When toxin neutralization assays were performed at higher toxin dose levels, the neutralizing capacity of individual antibodies was markedly reduced but this was largely compensated for by using two or more antibodies in combination, resulting in a potency of 79.4 IU/mg in the in vivo intradermal challenge assay. These recombinant antibody combinations are candidates for further clinical and regulatory development to replace equine DAT

A Randomized Controlled Dosing Study of Iyengar Yoga and Coherent Breathing for the Treatment of Major Depressive Disorder: Impact on Suicidal Ideation and Safety Findings

BACKGROUND: Yoga interventions offer promise for the treatment of major depressive disorder (MDD), yet their safety and potential impact on suicidal ideation (SI) have not been well documented. This study evaluated the safety of a randomized controlled dose-finding trial of Iyengar yoga plus coherent breathing for individuals with MDD, as well as the potential effects of the intervention on SI without intent. METHODS: Participants with Beck Depression Inventory-II (BDI-II) scores \u3e/=14 and a diagnosis of MDD (using DSM-IV criteria) were randomized to either a low dose group (LDG) or high dose group (HDG) and received a 12-week manualized intervention. The LDG included two 90-min yoga classes plus three 30-min homework sessions weekly. The HDG offered three 90-min classes plus four 30-min homework sessions weekly. RESULTS: Thirty-two individuals with MDD were randomized, of which 30 completed the protocol. At screening, SI without intent was endorsed on the BDI-II by 9 participants; after completing the intervention, 8 out of 9 reported resolution of SI. There were 17 adverse events possibly-related and 15 definitely-related to the intervention. The most common protocol-related adverse event was musculoskeletal pain, which resolved over the course of the study. CONCLUSIONS: The Iyengar yoga plus coherent breathing intervention was associated with the resolution of SI in 8 out of 9 participants, with mild side effects that were primarily musculoskeletal in nature. This preliminary evidence suggests that this intervention may reduce SI without intent and be safe for use in those with MDD

Liger for Next Generation Keck AO: Filter Wheel and Pupil Design

Liger is a next-generation near-infrared imager and integral field spectrograph (IFS) for the W.M. Keck Observatory designed to take advantage of the Keck All-Sky Precision Adaptive Optics (KAPA) upgrade. Liger will operate at spectral resolving powers between R$\sim$4,000 - 10,000 over a wavelength range of 0.8-2.4$\mu$m. Liger takes advantage of a sequential imager and spectrograph design that allows for simultaneous observations between the two channels using the same filter wheel and cold pupil stop. We present the design for the filter wheels and pupil mask and their location and tolerances in the optical design. The filter mechanism is a multi-wheel design drawing from the heritage of the current Keck/OSIRIS imager single wheel design. The Liger multi-wheel configuration is designed to allow future upgrades to the number and range of filters throughout the life of the instrument. The pupil mechanism is designed to be similarly upgradeable with the option to add multiple pupil mask options. A smaller wheel mechanism allows the user to select the desired pupil mask with open slots being designed in for future upgrade capabilities. An ideal pupil would match the shape of the image formed of the primary and would track its rotation. For different pupil shapes without tracking we model the additional exposure time needed to achieve the same signal to noise of an ideal pupil and determine that a set of fixed masks of different shapes provides a mechanically simpler system with little compromise in performance.Comment: 9 pages, 7 figures, 1 tabl

Liger for Next Generation Keck Adaptive Optics: Opto-Mechanical Dewar for Imaging Camera and Slicer

Liger is a next generation adaptive optics (AO) fed integral field spectrograph (IFS) and imager for the W. M. Keck Observatory. This new instrument is being designed to take advantage of the upgraded AO system provided by Keck All-Sky Precision Adaptive-optics (KAPA). Liger will provide higher spectral resolving power (R$\sim$4,000-10,000), wider wavelength coverage ($\sim$0.8-2.4 $\mu$m), and larger fields of view than any current IFS. We present the design and analysis for a custom-made dewar chamber for characterizing the Liger opto-mechanical system. This dewar chamber is designed to test and assemble the Liger imaging camera and slicer IFS components while being adaptable for future experiments. The vacuum chamber will operate below $10^{-5}$ Torr with a cold shield that will be kept below 90 K. The dewar test chamber will be mounted to an optical vibration isolation platform and further isolated from the cryogenic and vacuum systems with bellows. The cold head and vacuums will be mounted to a custom cart that will also house the electronics and computer that interface with the experiment. This test chamber will provide an efficient means of calibrating and characterizing the Liger instrument and performing future experiments.Comment: 8 pages, 6 figure

Conceptual hydrodynamic-thermal mapping modelling for coral reefs at south Singapore sea

YesCoral reefs are important ecosystems that not only provide shelter and breeding ground for many marine species, but can also control of carbon dioxide level in ocean and act as coastal protection mechanism. Reduction of coral reefs at Singapore coastal waters (SCW) region remains as an important study to identify the environmental impact from its busy industrial activities especially at the surrounding of Jurong Island in the south. This kind of study at SCW was often being related to issues such as turbidity, sedimentation, pollutant transport (from industry activities) effects in literatures, but seldom investigated from the thermal change aspect. In this paper, a computational model was constructed using the Delft3D hydrodynamic module to produce wave simulations on sea regions surrounding Singapore Island. The complicated semi-diurnal and diurnal tidal wave events experienced by SCW were simulated for 2 weeks duration and compared to the Admiralty measured data. To simulate the thermal mapping at the south Singapore coastal waters (SSCW) region, we first adapted a conversion of industrial to thermal discharge; then from the discharge affected area a thermal map was further computed to compare with the measured coral map. The outcomes show that the proposed novel thermal modelling approach has quite precisely simulated the coral map at SSCW, with the condition that the near-field thermal sources are considered (with the coverage area in the limit of 20 km × 20 km).The author also acknowledges the support of Nazarbayev University’s (Kazakhstan) research seed grant no. KF-12/6 for purchasing and providing the Delft3D software used in this study (which the author is the principal investigator of the grant

The ACS Survey of Galactic Globular Clusters XI: The Three-Dimensional Orientation of the Sagittarius Dwarf Spheroidal Galaxy and its Globular Clusters

We use observations from the ACS study of Galactic globular clusters to investigate the spatial distribution of the inner regions of the disrupting Sagittarius dwarf spheroidal galaxy (Sgr). We combine previously published analyses of four Sgr member clusters located near or in the Sgr core (M54, Arp 2, Terzan 7 and Terzan 8) with a new analysis of diffuse Sgr material identified in the background of five low-latitude Galactic bulge clusters (NGC 6624, 6637, 6652, 6681 and 6809) observed as part of the ACS survey. By comparing the bulge cluster CMDs to our previous analysis of the M54/Sgr core, we estimate distances to these background features. The combined data from four Sgr member clusters and five Sgr background features provides nine independent measures of the Sgr distance and, as a group, provide uniformly measured and calibrated probes of different parts of the inner regions of Sgr spanning twenty degrees over the face of the disrupting dwarf. This allows us, for the first time, to constrain the three dimensional orientation of Sgr's disrupting core and globular cluster system and compare that orientation to the predictions of an N-body model of tidal disruption. The density and distance of Sgr debris is consistent with models that favor a relatively high Sgr core mass and a slightly greater distance (28-30 kpc, with a mean of 29.4 kpc). Our analysis also suggests that M54 is in the foreground of Sgr by ~2 kpc, projected on the center of the Sgr dSph. While this would imply a remarkable alignment of the cluster and the Sgr nucleus along the line of sight, we can not identify any systematic effect in our analysis that would falsely create the measured 2 kpc separation. Finally, we find that the cluster Terzan 7 has the most discrepant distance (25 kpc) among the four Sgr core clusters, which may suggest a different dynamical history than the other Sgr core clusters.Comment: 41 pages, 16 figures, accepted to Ap

Panoramic SETI: on-sky results from prototype telescopes and instrumental design

The Panoramic SETI (Search for Extraterrestrial Intelligence) experiment (PANOSETI) aims to detect and quantify optical transients from nanosecond to second precision over a large field-of-view (∼4,450 square-degrees). To meet these challenging timing and wide-field requirements, the PANOSETI experiment will use two assemblies of ∼45 telescopes to reject spurious signals by coincidence detection, each one comprising custom-made fast photon-counting hardware combined with (f/1.32) focusing optics. Preliminary on-sky results from pairs of PANOSETI prototype telescopes (100 sq.deg.) are presented in terms of instrument performance and false alarm rates. We found that a separation of >1 km between telescopes surveying the same field-of-view significantly reduces the number of false positives due to nearby sources (e.g., Cherenkov showers) in comparison to a side- by-side configuration of telescopes. Design considerations on the all-sky PANOSETI instrument and expected field-of-views are reported