FDI in hot labour markets: The implications of the war for talent

This paper highlights an inherent contradiction that exists within investment promotion activities in rich countries. Since the financial crisis, many inward investment agencies have shifted their activities from job creation per se to seeking to attract investment in high-tech activities. Such knowledge-intensive sectors are engaged in what has become referred to as “the war for talent”, so locations need to understand their value proposition to firms, especially where labour is tight. This paper explores the implications of this, in terms of the impact on employment and earnings of high skilled labour. We show that, because skill shortages already exist in many of these sectors, seeking to attract inward investment in these sectors simply causes the earnings of such workers to be bid up, and employment in the incumbent sector to fall. We highlight the over-riding importance that firms place on the availability of skilled labour when determining locations, and how policies which promote labour market flexibility, particularly through investment in skills to address skill shortages, can significantly mitigate the adverse effects, which tend to be more keenly felt in poorer regions of Europe where skilled labour is in even shorter supply

Protease of bovine follicular fluid

Tato bakalářská práce se zabývá izolací a částečnou charakterizací proteáz folikulární tekutiny krávy. Folikulární tekutina byla nejprve rozdělena podle molekulových hmotností pomocí gelové chromatografie na Sephadexu G-100. Jako další metoda izolace byla zvolena ionexová chromatografie na Sephadexu DEAE. S výslednými frakcemi byla provedena SDS elektroforéza, stanovení proteolytické aktivity s proteolytickým substrátem azakaseinem; zymografie a diferenční SDS elektroforézy. Sebrané frakce folikulární tekutiny, stanovené metodou gelové chromatografie, měly relativní molekulové hmotnosti v rozmezí: 114 000 - 131 000 pro vzorek frakce I; 44 000 - 51 000 pro vzorek frakce II; 151 000 - 204 000 pro vzorek frakce III; 57 000 - 99 000 pro vzorek frakce IV; 14 000 - 38 000 pro vzorek frakce V; 180 - 500 pro vzorek frakce VI. Z výsledků proteolytické aktivity bylo rozhodnuto, že se nadále bude pracovat jen se vzorky frakcí III, IV a V, sebraných z gelové chromatografie a se vzorkem opožděné frakce PBS II, sebrané z ionexové chromatografie. Tyto vzorky vykazovaly nejvyšší aktivitu. Byly použity inhibitory, jimiž bylo možné určit, které typy proteáz se vyskytují ve vybraných frakcích. Ve frakci III, sebrané z gelové chromatografie, se vyskytovaly aspartátové proteázy, metalloproteázy a serinové proteázy,...This bachelor thesis deals with isolation and partial characterisation of cow protease follicular fluids. At first, the follicular fluid was isolated according to molecular weights by means of gel chromatography on Sephadex G-100. As another method of separation there was selected the ion exchange chromatography on Sephadex DEAE. For the characterization of resulting fractions SDS electrophoresis, assessment of proteolytic activity of the proteolytic substrate azakasein, zymography and differential SDS electrophoresis were used. The collected fractions of the follicular fluid defined by the gel chromatography method had the relative molecular weights raging 114 000 - 131 000 in fraction pattern I, 44 000 - 51 000 in fraction pattern II, 151 000 - 204 000 in fraction pattern III, 57 000 - 99 in fraction pattern IV, 14 000 - 38 000 in fraction pattern V and 180 - 500 in fraction pattern VI. From the results of the proteolytic activity I decided to work further only with fraction patterns III, IV and V collected from the gel chromatography and with the pattern of the delayed fraction PBS II collected from the ion exchange chromatography. These patterns reported the highest specific activity. Inhibitors were used to determine which type of protease occurs in thee chosen fractions. In the fraction...Department of BiochemistryKatedra biochemieFaculty of SciencePřírodovědecká fakult

Protease of bovine follicular fluid

This bachelor thesis deals with isolation and partial characterisation of cow protease follicular fluids. At first, the follicular fluid was isolated according to molecular weights by means of gel chromatography on Sephadex G-100. As another method of separation there was selected the ion exchange chromatography on Sephadex DEAE. For the characterization of resulting fractions SDS electrophoresis, assessment of proteolytic activity of the proteolytic substrate azakasein, zymography and differential SDS electrophoresis were used. The collected fractions of the follicular fluid defined by the gel chromatography method had the relative molecular weights raging 114 000 - 131 000 in fraction pattern I, 44 000 - 51 000 in fraction pattern II, 151 000 - 204 000 in fraction pattern III, 57 000 - 99 in fraction pattern IV, 14 000 - 38 000 in fraction pattern V and 180 - 500 in fraction pattern VI. From the results of the proteolytic activity I decided to work further only with fraction patterns III, IV and V collected from the gel chromatography and with the pattern of the delayed fraction PBS II collected from the ion exchange chromatography. These patterns reported the highest specific activity. Inhibitors were used to determine which type of protease occurs in thee chosen fractions. In the fraction..

Protease of bovine follicular fluid

This bachelor thesis deals with isolation and partial characterisation of cow protease follicular fluids. At first, the follicular fluid was isolated according to molecular weights by means of gel chromatography on Sephadex G-100. As another method of separation there was selected the ion exchange chromatography on Sephadex DEAE. For the characterization of resulting fractions SDS electrophoresis, assessment of proteolytic activity of the proteolytic substrate azakasein, zymography and differential SDS electrophoresis were used. The collected fractions of the follicular fluid defined by the gel chromatography method had the relative molecular weights raging 114 000 - 131 000 in fraction pattern I, 44 000 - 51 000 in fraction pattern II, 151 000 - 204 000 in fraction pattern III, 57 000 - 99 in fraction pattern IV, 14 000 - 38 000 in fraction pattern V and 180 - 500 in fraction pattern VI. From the results of the proteolytic activity I decided to work further only with fraction patterns III, IV and V collected from the gel chromatography and with the pattern of the delayed fraction PBS II collected from the ion exchange chromatography. These patterns reported the highest specific activity. Inhibitors were used to determine which type of protease occurs in thee chosen fractions. In the fraction..