Immunonutrition before esophagectomy: Impact on immune surveillance mechanisms

Preoperative oral immunonutrition was demonstrated to improve immune response and to decrease the infection rate in patients with cancer. This study aimed to assess how immunonutrition could influence the immune cell response in the mucosal microenvironment of esophageal adenocarcinoma. Therefore, A prospective cohort of consecutive patients undergoing esophagectomy for esophageal adenocarcinoma was enrolled. A subgroup of them was given preoperative oral immunonutrition with Oral Impact and was compared to those who received no preoperative supplementation. Mucosal samples from healthy esophagus were obtained at esophagectomy. Histology, immunohistochemistry, gene expression analysis, and cytofluorimetry were performed. Markers of activation of antigen-presenting cells (CD80, CD86, and HLA-I), innate immunity (TLR4 and MyD88), and cytotoxic lymphocyte infiltration and activation (CD8, CD38, CD69, and CD107) were measured. In all, 50 patients received preoperative Oral Impact and 129 patients received no nutritional support. CD80, CD86, MyD88, and CD69 messenger RNA expression was significantly increased in patients receiving immunonutrition compared to controls. In the subgroup of patients with stages I-II cancer, the rate of epithelial cells expressing CD80 and HLA-ABC was significantly higher in those receiving immunonutrition compared to controls as well as CD8+ CD28+ cell rate. Immunonutrition administration before surgery was significantly associated to increased degranulating CD8 and natural killer cells (CD107+) infiltrating the healthy esophageal mucosa. All the comparisons were adjusted for cancer stage and preoperative therapy. In conclusion, in healthy esophageal mucosa of patients undergoing esophagectomy, a 5-day course of immunonutrition enhances expression of antigen-presenting cells activity and increased CD8+ T cell activation and degranulating activity. Further studies are warranted to understand the clinical implication in terms of cancer recurrence

Spectroscopy of 10 gamma-ray BL Lac objects at high redshift

We present high S/N optical spectra of 10 BL Lac objects detected at GeV energies by Fermi satellite (3FGL catalog), for which previous observations suggested that they are at relatively high redshift. The new observations, obtained at the 10 m Gran Telescopio Canarias, allowed us to find the redshift for J0814.5+2943 (z = 0.703) and we can set spectroscopic lower limit for J0008.0+4713 (z>1.659) and J1107.7+0222 (z>1.0735) on the basis of Mg II intervening absorption features. In addition we confirm the redshifts for J0505.5+0416 (z=0.423) and for J1450+5200 (z>2.470). Finally we contradict the previous z estimates for five objects (J0049.7+0237, J0243.5+7119, J0802.0+1005, J1109.4+2411, and J2116.1+3339).Comment: 10 pages, 2 figures, 4 tables; accepted for publication in Ap

On the redshift of the very high energy {\gamma}-ray BL Lac object S2 0109+22

The determination of the redshift of extragalactic gamma ray sources is of fundamental importance for the interpretation of their emission models and extragalactic population studies, AGN classification schemes, and to study the interaction with the extragalactic background light. Here we focus on the BL Lac S2 0109+22, recently detected at very high energies. We obtained a high signal-to-noise optical spectrum at Gran Telescopio Canarias for the source over the spectral range 4000-10000 Angstrom, to search for spectral features of the host galaxy and/or of the nuclear source and it was compared with previous observations in order to confirm and/or dispute its redshift determination. We find the S2 0109+22 optical spectrum is well represented by a power law continuum without any absorption or emission feature of Equivalent Width >0.1 Angstrom. Based on the new optical spectrum we are able to set a redshift lower limit z>0.35, assuming the source is hosted by a massive elliptical galaxy typical for this class of sources. The redshift z=0.265 proposed in the literature for this object is most probably referred to a r=18.3 galaxy at 15" from S2 0109. This galaxy could belong to a group of faint galaxies located at z~0.26.Comment: 5 pages, 5 figures; accepted on MNRA

Mitochondrial DNA reveals genetic structuring of <i>Pinna nobilis</i> across the Mediterranean Sea

Pinna nobilis is the largest endemic Mediterranean marine bivalve. During past centuries, various human activities have promoted the regression of its populations. As a consequence of stringent standards of protection, demographic expansions are currently reported in many sites. The aim of this study was to provide the first large broad-scale insight into the genetic variability of P. nobilis in the area that encompasses the western Mediterranean, Ionian Sea, and Adriatic Sea marine ecoregions. To accomplish this objective twenty-five populations from this area were surveyed using two mitochondrial DNA markers (COI and 16S). Our dataset was then merged with those obtained in other studies for the Aegean and Tunisian populations (eastern Mediterranean), and statistical analyses (Bayesian model-based clustering, median-joining network, AMOVA, mismatch distribution, Tajima’s and Fu’s neutrality tests and Bayesian skyline plots) were performed. The results revealed genetic divergence among three distinguishable areas: (1) western Mediterranean and Ionian Sea; (2) Adriatic Sea; and (3) Aegean Sea and Tunisian coastal areas. From a conservational point of view, populations from the three genetically divergent groups found may be considered as different management units

EUREKA study - The evaluation of real-life use of a biophotonic system in chronic wound management: An interim analysis

Objective: Interest has grown regarding photobiomodulation (PBM) with low-level light therapy, which has been shown to positively affect the stages of the wound healing process. In a real-life context clinical setting, the objective of the EUREKA study was to investigate efficacy, safety, and quality of life associated with the use of a BioPhotonic gel (LumiHeal\u2122) in the treatment of chronic wounds such as venous leg ulcers (VLUs), diabetic foot ulcers (DFUs), and pressure ulcers (PUs). This BioPhotonic gel represents a new, first-in-class emission spectrum of light, including fluorescence, to induce PBM and modulate healing. Design: The multicenter, prospective, interventional, uncontrolled, open-label study enrolled 100 patients in 12 wound centers in Italy. We performed an early interim analysis based on the first 33 subjects (13 VLU, 17 DFU, 3 PU) in seven centers who completed the study. Main results: Seventeen patients (52%) achieved total wound closure (full re-epithelialization for 2 weeks) during the study period. Two patients (6%) were considered \u201calmost closed\u201d (decrease of the wound area of more than 90% at study end) and three others (9%) were considered \u201cready for skin grafting\u201d. No related serious adverse events were observed, and the compliance was excellent. After the treatment, the average time to \u201cpain-free\u201d was 11.9 days in the VLU group. Quality of life was improved with overall increase of 26.4% of the total score (Cardiff Wound Impact Schedule, p=0.001). Conclusion: The study revealed a positive efficacy profile of the BioPhotonic gel in promoting wound healing and reactivating the healing process in different types of chronic, hard-to-heal wounds. The treatment was shown to be safe and well tolerated by the patients, and a reduction of pain perception was also detected during the treatment period. The improvement of the quality of life was accompanied by a high level of clinician satisfaction

The molecular landscape of colitis-associated carcinogenesis

In spite of the well-established histopathological phenotyping of IBD-associated preneoplastic and neoplastic lesions, their molecular landscape remains to be fully elucidated. Several studies have pinpointed the initiating role of longstanding/relapsing inflammatory insult on the intestinal mucosa, with the activation of different pro-inflammatory cytokines (TNF-\u3b1, IL-6, IL-10, IFN-\u3b3), chemokines and metabolites of arachidonic acid resulting in the activation of key transcription factors such as NF-\u3baB. Longstanding inflammation may also modify the intestinal microbiota, prompting the overgrowth of genotoxic microorganisms, which may act as further cancer promoters. Most of the molecular dysregulation occurring in sporadic colorectal carcinogenesis is documented in colitis-associated adenocarcinoma too, but marked differences have been established in both their timing and prevalence. Unlike sporadic cancers, TP53 alterations occur early in IBD-related carcinogenesis, while APC dysregulation emerges mainly in the most advanced stages of the oncogenic cascade. From the therapeutic standpoint, colitis-associated cancers are associated with a lower prevalence of KRAS mutations than the sporadic variant. Epigenetic changes, including DNA methylation, histone modifications, chromatin remodeling, and non-coding RNAs, are significantly involved in colitis-associated cancer development and progression. The focus now is on identifying diagnostic and prognostic biomarkers, with a view to ultimately designing patient-tailored therapie

Involvement of pro-inflammatory cytokines and growth factors in the pathogenesis of Dupuytren's contracture: a novel target for a possible future therapeutic strategy?

Dupuytren's contracture (DC) is a benign fibro-proliferative disease of the hand causing fibrotic nodules and fascial cords which determine debilitating contracture and deformities of fingers and hands. The present study was designed to characterize pro-inflammatory cytokines and growth factors involved in the pathogenesis, progression and recurrence of this disease, in order to find novel targets for alternative therapies and strategies in controlling DC. The expression of pro-inflammatory cytokines and of growth factors was detected by immunohistochemistry in fibrotic nodules and normal palmar fascia resected respectively from patients affected by DC and carpal tunnel syndrome (CTS; as negative controls). Reverse transcription (RT)-PCR analysis and immunofluorescence were performed to quantify the expression of transforming growth factor (TGF)-β1, interleukin (IL)-1β and vascular endothelial growth factor (VEGF) by primary cultures of myofibroblasts and fibroblasts isolated from Dupuytren's nodules. Histological analysis showed high cellularity and high proliferation rate in Dupuytren's tissue, together with the presence of myofibroblastic isotypes; immunohistochemical staining for macrophages was completely negative. In addition, a strong expression of TGF-β1, IL-1β and VEGF was evident in the extracellular matrix and in the cytoplasm of fibroblasts and myofibroblasts in Dupuytren's nodular tissues, as compared with control tissues. These results were confirmed by RT-PCR and by immunofluorescence in pathological and normal primary cell cultures. These preliminary observations suggest that TGF-β1, IL-1β and VEGF may be considered potential therapeutic targets in the treatment of Dupuytren's disease (DD)

Buone pratiche per la tutela della qualità dell’aria indoor. L’esperienza del Treno Verde 2017

La qualità dell’aria ed in particolare la qualità dell’aria all’interno degli edifici, nell’ambito dei temi relativi a salute e benessere della popolazione, assume oggi, importanza sempre più rilevante. I principali problemi legati alla qualità dell'aria Indoor - IAQ (Indoor Air Quality) - sono strettamente correlati alla concentrazione e definizione dei principali composti inquinanti presenti negli ambienti confinati. L’irrinunciabile tema del risparmio energetico ha prodotto precauzioni che hanno condotto, oltre a un maggior isolamento degli edifici ed a una diminuzione dei tassi di ventilazione, anche all’utilizzo di nuovi materiali e di nuove apparecchiature, cioè a misure che aumentano le concentrazioni di agenti inquinanti che si formano nei locali. Nel corso degli ultimi decenni si è difatti assistito a un progressivo deterioramento della qualità dell’aria negli ambienti confinati. Numerosi studi scientifici hanno dimostrato la presenza, nell’aria degli ambienti di vita, di agenti inquinanti a bassa concentrazione di difficile misurazione che possono determinare effetti sulla salute non ancora completamente noti. Gli inquinanti indoor, che possono agire singolarmente o combinati con altri fattori, determinano una diminuzione del comfort ambientale e un rischio per la salute; sono agenti di tipo chimico (composti organici e inorganici), fisico (radiazioni ionizzanti e non ionizzanti) e biologico (microrganismi, muffe, acari). Considerato che gran parte della popolazione trascorre il proprio tempo in ambienti confinati, l’esposizione all’inquinamento indoor è dominante rispetto a quella outdoor. Vengono considerati come sintomi specifici, non gravi, che possono impattare sulla salute e quindi, sui costi sociali del paese: malattie respiratorie trasmissibili, allergie e asma, sintomi della sindrome dell’edificio malato (SBS, dall’inglese Sick Building Syndrome)

Low-power Radio Galaxies in the Distant Universe: A search for FRI at 1<z<2 in the COSMOS field

We present a search for FRI radio galaxies between 1 < z < 2 in the COSMOS field. In absence of spectroscopic redshift measurements, the selection method is based on multiple steps which make use of both radio and optical constraints. The basic assumptions are that 1) the break in radio power between low-power FRIs and the more powerful FRIIs does not change with redshift, and 2) that the photometric properties of the host galaxies of low power radio galaxies in the distant universe are similar to those of FRIIs in the same redshift bin, as is the case for nearby radio galaxies. We describe the results of our search, which yields 37 low-power radio galaxy candidates that are possibly FRIs. We show that a large fraction of these low-luminosity radio galaxies display a compact radio morphology, that does not correspond to the FRI morphological classification. Furthermore, our objects are apparently associated with galaxies that show clear signs of interactions, at odds with the typical behavior observed in low-z FRI hosts. The compact radio morphology might imply that we are observing intrinsically small and possibly young objects, that will eventually evolve into the giant FRIs we observe in the local universe. One of the objects appears as point-like in HST images. This might belong to a population of FRI-QSOs, which however would represent a tiny minority of the overall population of high-z FRIs. As for the local FRIs, a large fraction of our objects are likely to be associated with groups or clusters, making them "beacons" for high redshift clusters of galaxies. Our search for candidate high-z FRIs we present in this paper constitutes a pilot study for objects to be observed with future high-resolution and high-sensitivity instruments (shortened)Comment: 44 pages, 11 figures, version accepted for publication in Ap