Sex Steroids in Insects and the Role of the Endosymbiont Wolbachia:A New Perspective

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The calculation of Solvency Capital Requirement using Copulas

Our aim is to present an alternative methodology to the standard formula imposed to the insurance regulation (the European directive knows as Solvency II) for the calculus of the capital requirements. We want to demonstrate how this formula is now obsolete and how is possible to obtain lower capital requirement through the theory of the copulas, function that are gaining increasing importance in various economic areas. A lower capital requirement involves the advantage for the various insurance companies not to have unproductive capital that can therefore be used for the production of further profits. Indeed the standard formula is adequate only with some particular assumptions, otherwise it can overestimate the capital requirements that are actually needed as the standard formula underestimates the effect of diversification

Progressive Collapse Analysis of the Champlain Towers South in Surfside, Florida

Since the Ronan Point collapse in the UK in 1968, the progressive collapse analysis of residential buildings has gradually drawn the attention of civil engineers and the scientific community. Recent advances in computer science and the development of new numerical methodologies allow us to perform high-fidelity collapse simulations. This paper assesses different scenarios that could have hypothetically caused the collapse of the Champlain Tower South Condo in Surfside, Florida, in 2021, one of the most catastrophic progressive collapse events that has ever occurred. The collapse analysis was performed using the latest developments in the Applied Element Method (AEM). A high-fidelity numerical model of the building was developed according to the actual structural drawings. Several different collapse hypotheses were examined, considering both column failures and degradation scenarios. The analyses showed that the failure of deep beams at the pool deck level, directly connected to the perimeter columns of the building, could have led to the columns’ failure and subsequent collapse of the eastern wing of the building. The simulated scenario highlights the different stages of the collapse sequence and appears to be consistent with what can be observed in the footage of the actual collapse. To improve the performance of the structure against progressive collapse, two modifications to the original design of the building were introduced. From the analyses, it was found that disconnecting the pool deck beam from the perimeter columns could have been effective in preventing the local collapse of the pool deck slab from propagating to the rest of the building. Moreover, these analyses indicate that enhancing the torsional strength and stiffness of the core could have prevented the collapse of the eastern part of the building, given the assumptions and initiation scenarios considered

The Correlation between Retinal and Choroidal Thickness with Age-Related White Matter Hyperintensities in Progressive Supranuclear Palsy

Choroid; Optical coherence tomography; RetinaCoroide; Tomografia de coherència òptica; RetinaCoroides; Tomografía de coherencia óptica; RetinaProgressive supranuclear palsy (PSP) is a rare neurodegenerative disease. Recently, several retinal layers in PSP compared to healthy controls. were found to be thinner. However, no studies evaluating the correlation between retinal layers and cerebral white matter changes, nor eventual choroidal changes in PSP, have been conducted so far. The goals of the present study were to explore potential differences in choroidal structure between PSP and healthy controls, and to describe the relationship between retinal layers’ thickness and volume, using spectral-domain optical coherence tomography (SD-OCT) and age-related white matter change scores (ARWMC) using magnetic resonance imaging (MRI) of the brain. Choroidal structures of 26 PSP patients and 26 healthy controls using standard SD-OCT with an enhanced depth imaging (EDI) approach were analyzed; then, retinal the structures of 16 of these PSP patients using standard SD-OCT were examined; finally, the same patients underwent brain MRI, and their cerebral white matter changes were calculated. Non-statistically significant differences between PSP patients’ and healthy controls’ choroidal structure were found. On the contrary, PSP patients’ inner retinal layers (INR), retinal pigmented epithelium (RPE) and all retinal layers’ thicknesses in the macular region were found to be significantly correlated with ARWMC, independently from age and axial length (AL). PSP patients’ neurological alterations go hand in hand with retinal ones, independently from age and axial length. Our results suggest a mutual relationship between cerebral and retinal structure pathological alterations. On the other hand, no significant differences in the choroidal evaluation compared to healthy controls have been found

miR-155 is positively regulated by CBX7 in mouse embryonic fibroblasts and colon carcinomas, and targets the KRAS oncogene

Background: Loss of CBX7 expression has been described in several malignant neoplasias, including human colon and thyroid carcinomas proposing CBX7 as a tumor suppressor gene with a key role in cancer progression. This role is supported from the development of benign and malignant neoplasias in Cbx7 null mice. The aim of our work has been to investigate the mechanisms underlying the CBX7 oncosuppressor activity by analyzing the microRNAs (miRNAs) regulated by CBX7. Methods: The miRNA expression profiles of the mouse embryonic fibroblasts (MEFs) null for Cbx7 and the wild-type counterpart were analyzed by the miRNACHIP microarray and then validated by qRT-PCR. To asses KRAS as target of miR-155 we evaluated the protein levels after transfection of the synthetic miR-155. Human colon carcinoma samples have been investigated for the expression of CBX7 and miR-155. Results: Twenty miRNAs were found upregulated and nine, including miR-155, downregulated in cbx7-null MEFS in comparison with the wild-type ones. Then, we focused on miR-155 since several studies have shown its deregulated expression in several human malignancies and, moreover, was the most downregulated miRNA. Subsequently, we searched for miR-155 target genes demonstrating that KRAS protein levels are directly modulated by miR-155. A direct significant correlation (r = 0.6779) between CBX7 and miR-155 expression levels was found in a set of human colon carcinoma tissue samples. Conclusion: miR-155 is positively regulated by CBX7 in MEFs and colon carcinomas, and has KRAS as one of the target genes likely accounting for the anti-apoptotic activity ascribed to miR-155 in some tissue contexts

A methodology to quantify debris generation after a seismic event

Seismic damage simulation at the regional scale can potentially provide valuable information that can facilitate decision making, enhance planning for disaster mitigation, and reduce human and economic losses. When an earthquake happens, building damage assessment is one of the important issues in earthquake loss estimation. The amount of debris generated and the effects on related critical infrastructures is also an essential information to evaluate. Indeed, as cascading consequence of debris accumulation, the road network can be interrupted. This entails an overall increase in the average number of people who have difficulty evacuating, with high risk that residents cannot evacuate any areas. This study proposes a method to evaluate the debris affected area and the debris amount as a function of the geometric characteristics and the level of damage of the buildings. The first part of this work is focused on the evaluation of the debris area’s extension by numerical simulations. Comparison of the results with images of real seismic damaged structures allows the validation of the results. Besides, experimental tests on a small shaking table are performed to validate the numerical simulations. A mathematic model based on the results is also proposed

The role of HMGA1 protein in gastroenteropancreatic neuroendocrine tumors

Neuroendocrine tumors (NETs) are neoplasms derived from neuroendocrine cells. One of their main features is to often remain asymptomatic and clinically undetectable. High Mobility Group A (HMGA) proteins belong to a family of non-histone chromatinic proteins able to modulate gene expression through the interaction with DNA and transcription factors. They are overexpressed in most of the human malignancies, playing a critical role in carcinogenesis. However, their expression levels and their role in neuroendocrine carcinogenesis has not been exhaustively evaluated until now. Therefore, in this study, we have addressed the validity of using the expression of HMGA1 as a diagnostic marker and have investigated its role in NET carcinogenesis. The expression of HMGA1 has been evaluated by qRT-PCR and immunohistochemistry, using NET tissue microarrays, in a cohort of gastroenteropancreatic (GEP)-NET samples. The expression levels of HMGA1 have been then correlated with the main clinical features of NET samples. Finally, the contribution of HMGA1 overexpression to NET development has been addressed as far as the modulation of proliferation and migration abilities of NET cells is concerned. Here, we report that HMGA1 is overexpressed in GEP-NET samples, at both mRNA and protein levels, and that the silencing of HMGA1 protein expression interferes with the ability of NET cells to proliferate and migrate through the downregulation of Cyclin E, Cyclin B1 and EZH2. These results propose the HMGA proteins as new diagnostic and prognostic markers