INTEGRAÇÃO CURRICULAR NO IFES: LIMITES E POSSIBILIDADES DA PESQUISA-AÇÃO

Neste trabalho, foi analisado a pesquisa-ação no contexto da integração curricular do ensino médio à educação profissional na Rede Federal. Identificou-se a emergência de uma “resistência muda” dos sujeitos envolvidos que por meio de discursos e práticas destoantes apoiaram, mas também boicotaram a pesquisaação, resultando na descontinuidade da mobilização e do envolvimento dos grupos focais. De todo modo, houve êxito no campo do diagnóstico e da formação docente e os resultados futuros são promissores para os objetivos do estudo

Use of Bone Marrow Aspirate Concentrate (BMAC) Associated with Hyperbaric Oxygenation Therapy in Maxillary Appositional Bone Reconstruction. A Randomized Clinical Trial

Objectives: The objective of this study was to evaluate bone reconstruction using xenograft alone and associated with bone marrow aspirate concentrate (BMAC) and hyperbaric oxygen therapy. Material and Methods: Twenty-four maxillary edentulous patients were randomly assigned into three groups: Control group (CG)—xenograft bone alone (n = 8); Group 1 (G1)—xenogeneic bone block combined with BMAC (n = 8), and Group 2 (G2)—xenogeneic bone block combined with BMAC and hyperbaric oxygenation (n = 8). Bone biopsies were harvested 6 months after grafting. Vital Mineralized Tissue (VMT), Non-vital Mineralized Tissue (NVMT), and Non-Mineralized Tissue (NMT) were measured. Computed tomography was also performed on three occasions T0 (preoperative), T4 (4 months postoperative), and T8 (8 months postoperative). The difference between T4 and T8 values with respect to T0 was used to determine the thickness level gain after 4 and 8 months, respectively. Results: The tomographic evaluation did not show significant differences between the groups either at 4 or at the 8 months postoperatively. Regarding the histomorphometric analysis, CG had the lowest percentages of VMT (36.58 ± 9.56%), whereas G1 and G2 had similar results (55.64 ± 2.83% and 55.30 ± 1.41%, respectively). Concerning NMT and NVMT levels, the opposite was observed, with CG levels of 51.21 ± 11.54% and 11.16 ± 2.37%, G1 of 39.76 ± 11.48% and 3.65 ± 0.87%, and G2 of 40.3 ± 11.48% and 4.10 ± 0.87%, respectively. Conclusions: The use of bone block xenograft associated with BMAC resulted in a significant increase of bone neoformation when compared to the xenograft alone, though hyperbaric oxygenation did not enhance the results.Odontologí

CAPACIDADES DINÂMICAS BASEADAS EM CONHECIMENTO E TIPOS DE INOVAÇÃO: PROPOSIÇÃO DE UM FRAMEWORK DE ANÁLISE

As Capacidades Dinâmicas (CD) consistem um conceito bastante desenvolvido e pesquisado na área de Estratégia, mas que, no entanto, evidenciam uma falta de uniformidade em sua definição e dimensões componentes quando abordadas pelos diferentes autores. Esta pesquisa tem como objetivo propor um framework de análise das Capacidades Dinâmicas Baseadas em Conhecimento (CDBC) e os distintos tipos de Inovação, construído a partir da síntese de estudos tipo survey desenvolvidos analisando a relação entre as CDBC e desempenho de inovação. Partindo-se das categorias identificados por Denford (2013) para compreensão deste e por meio da estratégia de análise de conteúdo, buscou-se identificar a dimensões associadas, de que forma foram operacionalizadas e que tipo de relação foi encontrada. Como resultado, após discussão das convergências e divergências entre as abordagens de CDBC, este trabalho propõe um framework para orientar pesquisas futuras entre CDBC e Inovação, o Modelo Unificado das Capacidades Dinâmicas Baseadas em Conhecimento. The Dynamic Capabilities (DC) are one of the most important and researched topic in the Strategy field. However, there is some lack of uniformity about your definition and distinguished dimensions are used when this theme is presented by different authors. This research aim to propose an analytic framework of Knowledge-Based Dynamic Capabilities (KDBC) and types of innovation, developed from the synthesis of survey studies that relate KDBC and Innovation Performance. From Denford (2013) categories proposal, and using content analysis strategy, this study identified the associated dimensions, how they were operationalized and the type of relationship. As result, this paper introduces a framework to better understand the relationship between the different types of Knowledge-Based Dynamic Capabilities and Innovation, and can guide future research in the field.

ODINOFAGIA EM PACIENTE HIV

Caso Clínico: Homem, 39 anos, com diagnóstico recente de infecção pelo vírus da imunideficiência humana (HIV), com CD4 de 112 células/mm3, em uso de tenofovir, lamivudina e efavirenz, foi admitido no Hospital de Clínicas por insuficiência renal aguda provavelmente relacionada a antirretroviral. Durante internamento, queixava-se de odinofagia, febre noturna, linfonodomegalia generalizada, pancitopenia, e lesões violáceas em membros inferiores, além de relatos de perda de peso. Feito um curso de 7 dias de fluconazol, sem melhora clínica. Realizado então endoscopia digestiva alta, que mostrou a seguinte imagem

15-Deoxy-Δ 12,14

The cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-γ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ2 decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ2 downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ2 shows the potential to become a new therapeutic approach for thyroid tumors

The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain

An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was transferred, by electroporation assay, to non-pathogenic receptor strains (E. coli strains K12 MS101 and HB101), resulting in transformant strains A and B, respectively. These strains exhibited adhesion properties to in vitro cultivated HEp-2 cells but did not have the capacity for invasion. The adherence occurred despite the absence of fimbriae; this finding suggests that the 88 MDa plasmid has afimbrial adhesin genes

Impact of COVID-19 In-hospital Mortality in Chagas Disease Patients

The COVID-19 virus infection caused by the new SARS-CoV-2 was first identified in Rio de Janeiro (RJ), Brazil, in March 2020. Until the end of 2021, 504,399 COVID-19 cases were confirmed in RJ, and the total death toll reached 68,347. The Evandro Chagas National Institute of Infectious Diseases from Oswaldo Cruz Foundation (INI-Fiocruz) is a referral center for treatment and research of several infectious diseases, including COVID-19 and Chagas disease (CD). The present study aimed to evaluate the impact of COVID-19 on in-hospital mortality of patients with CD during the COVID-19 pandemic period. This observational, retrospective, longitudinal study evaluated all patients with CD hospitalized at INI-Fiocruz from May 1, 2020, to November 30, 2021. One hundred ten hospitalizations from 81 patients with CD (58% women; 68 ± 11 years) were evaluated. Death was the study's main outcome, which occurred in 20 cases. The mixed-effects logistic regression was performed with the following variables to test whether patients admitted to the hospital with a COVID-19 diagnosis would be more likely to die than those admitted with other diagnoses: admission diagnosis, sex, age, COVID-19 vaccination status, CD clinical classification, and the number of comorbidities. Results from multiple logistic regression analysis showed a higher risk of in-hospital mortality in patients diagnosed with COVID-19 (OR 6.37; 95% CI 1.78–22.86) compared to other causes of admissions. In conclusion, COVID-19 infection had a significant impact on the mortality risk of INI-Fiocruz CD patients, accounting for one-third of deaths overall. COVID-19 presented the highest percentage of death significantly higher than those admitted due to other causes during the COVID-19 pandemic