Evaluation of two enzyme-linked immunosorbent assays for diagnosis of bluetongue virus in wild ruminants

Bluetongue (BT) is a reportable re-emerging vector-borne disease of animal health concern. Enzyme-linked immunosorbent assays (ELISA) are frequently used in BT surveillance programs in domestic ruminants, but their diagnostic accuracy has not been evaluated for wild ruminants, which can play an important role as natural reservoirs of bluetongue virus (BTV). The aim of this study was to assess two commercial ELISAs for BT diagnosis in wild ruminants using control sera of known BTV infection status and field samples. When control sera were tested, the double recognition ELISA (DR-ELISA) showed 100 % sensitivity (Se) and specificity (Sp), while the competitive ELISA (C-ELISA) had 86.4 % Se and 97.1 % Sp. Using field samples, the selected latent-class analysis model showed 95.7 % Se and 85.9 % Sp for DR-ELISA, 58.2 % Se and 95.8 % Sp for C-ELISA and 84.2 % Se for the serum neutralization test (SNT). Our results indicate that the DR-ELISA may be a useful diagnostic method to assess BTV circulation in endemic areas, while the C-ELISA should be selected when free-areas are surveyed. The discrepancy between control and field samples point out that the inclusion of field samples is required to assess the accuracy of commercial ELISAs for the serological diagnosis of BTV in wild ruminants.info:eu-repo/semantics/acceptedVersio

Description of the first Schmallenberg disease outbreak in Spain and subsequent virus spreading in domestic ruminants

Schmallenberg disease (SBD) is an emerging disease transmitted mainly among ruminant species by biting midges of the genus Culicoides. Since the Schmallenberg virus (SBV) was first identified in Germany in late 2011, it rapidly spread to other European countries. The aims of the present study were to describe the first SBD outbreak in Spain and to assess the spread and risk factors associated with SBV infection in domestic ruminants from nearby farms during the following year. In March 2012, one malformed stillborn lamb from a sheep farm located in Cordoba province (Southern Spain) was subjected to necropsy. Pathological compatible lesions and molecular analyses confirmed the first SBV infection in Spain. Afterwards, serum samples from 505 extensively reared domestic ruminants from 29 farms were analysed using both blocking ELISA and virus neutralization test against SBV. The overall seroprevalence was 54.4% (CI95%: 50.0–58.7). Antibodies were detected in 70.6%, 46.0% and 34.8% of cattle, sheep and goats, respectively. A generalized estimating equation model indicated that the main risk factors associated with SBV infection were: species (cattle), age (adult), and absence of animal insecticide treatment. Pathological and molecular results confirmed the presence of SBV in Spain few months after it was firstly identified in Germany. The seroprevalence detected indicates a widespread circulation of SBV in nearby domestic ruminant farms one year after this first outbreak was reported in Spain. Further studies are warranted to determine the spatio-temporal trend of SBV in domestic ruminants in this country.info:eu-repo/semantics/acceptedVersio

Extinction risk of Mesoamerican crop wild relatives

Ensuring food security is one of the world's most critical issues as agricultural systems are already being impacted by global change. Crop wild relatives (CWR)—wild plants related to crops—possess genetic variability that can help adapt agriculture to a changing environment and sustainably increase crop yields to meet the food security challenge. Here we report the results of an extinction risk assessment of 224 wild relatives of some of the world's most important crops (i.e. chilli pepper, maize, common bean, avocado, cotton, potato, squash, vanilla and husk tomato) in Mesoamerica—an area of global significance as a centre of crop origin, domestication and of high CWR diversity. We show that 35% of the selected CWR taxa are threatened with extinction according to The International Union for Conservation of Nature (IUCN) Red List demonstrates that these valuable genetic resources are under high anthropogenic threat. The dominant threat processes are land use change for agriculture and farming, invasive and other problematic species (e.g. pests, genetically modified organisms) and use of biological resources, including overcollection and logging. The most significant drivers of extinction relate to smallholder agriculture—given its high incidence and ongoing shifts from traditional agriculture to modern practices (e.g. use of herbicides)—smallholder ranching and housing and urban development and introduced genetic material. There is an urgent need to increase knowledge and research around different aspects of CWR. Policies that support in situ and ex situ conservation of CWR and promote sustainable agriculture are pivotal to secure these resources for the benefit of current and future generations

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p < 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

Dental microwear analysis in Gliridae (Rodentia): methodological issues and paleodiet inferences based on Armantomys from the Madrid Basin (Spain)

In the present study we analyze the microwear of the species included in the lineage Armantomys aragonensis-A. tricristatus of two samples from two middle Miocene localities from the Madrid Basin (El Cañaveral and Casa Montero). The methodological part of the study compares light stereomicroscope photographs from resin casts and Environmental Scanning Electron Microscope (ESEM) photographs from original material. Furthermore two regions of the tooth crown (the most lingual part of the anteroloph, and the most lingual part of the protoloph) and two homologous regions of first and second upper molars have been analyzed. The paleoecological part includes the results of the microwear analyses of the two species included in the lineage A. aragonensis-A. tricristatus. The replacement of A. aragonensis with A. tricristatus in the Madrid Basin occurred during the Middle Miocene Climate Transition (MMCT) and is marked by a change in dental morphology. Therefore, in order to infer changes in diet associated to a shift in the environment, we checked for correlations on microwear features between the two different morphologies of the species. The two methodologies tested, ESEM on original teeth and light stereomicroscopy on resin casts, showed similar results on microwear analysis in the glirid Armantomys. Besides, both regions of the tooth crown and dental elements showed the same microwear patterns. The substitution of the species included in the lineage Armantomys aragonensis-A. tricristatus might not imply a change in diet

Dietary adaptations and tooth morphology in squirrels: Insights from extant and extinct species

An important part of the ecological evolution of many lineages consists of adaptations to new diets as climate and available resources changed. In this study, we inferred when squirrels adapted morphologically to new diets by analyzing data from both extant and extinct species. We relied on the relationship between tooth morphology in extant squirrels and their dietary preferences to infer the diets of extinct species. To achieve this, we employed two approaches, namely geometric morphometrics and Elliptic Fourier analyses, to measure the outline of the fourth lower premolar (p4) in occlusal view. We then used both datasets to infer the diets of extinct species. Additionally, we used phylogenetic comparative methods on extant data to explore the evolution of diet and tooth size, identifying potential drivers of squirrel morphological evolution in the context of past climatic changes. Our findings indicate that the morphology of squirrel teeth has been maintained through the evolutionary history of most lineages, but that it also has changed in some lineages as a result of adaptations to particularly demanding diets, sometimes mediated by climatic factors, which have played a crucial role in shaping tooth morphology. Furthermore, we demonstrated that both geometric morphometrics and Elliptic Fourier analyses can yield a similar percentage of correct classifications and congruent dietary inferences. This study provides valuable insights into the macroevolutionary changes in squirrels and provides further evidence of the usefulness of tooth morphology for inferring the diets of extinct mammalian species.Universidad Complutense de MadridAmerican Museum of Natural HistoryAlexander von Humboldt FoundationMCIN/AEI/10.13039/501100011033European Union Next Generation EU/PRTRMinisterio de Ciencia, Innovación y Universidades-Agencia Estatal de InvestigaciónDepto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEpu

Description of the first Schmallenberg disease outbreak in Spain and subsequent virus spreading in domestic ruminants

Schmallenberg disease (SBD) is an emerging disease transmitted mainly among ruminant species by biting midges of the genus Culicoides. Since the Schmallenberg virus (SBV) was first identified in Germany in late 2011, it rapidly spread to other European countries. The aims of the present study were to describe the first SBD outbreak in Spain and to assess the spread and risk factors associated with SBV infection in domestic ruminants from nearby farms during the following year. In March 2012, one malformed stillborn lamb from a sheep farm located in Cordoba province (Southern Spain) was subjected to necropsy. Pathological compatible lesions and molecular analyses confirmed the first SBV infection in Spain. Afterwards, serum samples from 505 extensively reared domestic ruminants from 29 farms were analysed using both blocking ELISA and virus neutralization test against SBV. The overall seroprevalence was 54.4% (CI95%: 50.0–58.7). Antibodies were detected in 70.6%, 46.0% and 34.8% of cattle, sheep and goats, respectively. A generalized estimating equation model indicated that the main risk factors associated with SBV infection were: species (cattle), age (adult), and absence of animal insecticide treatment. Pathological and molecular results confirmed the presence of SBV in Spain few months after it was firstly identified in Germany. The seroprevalence detected indicates a widespread circulation of SBV in nearby domestic ruminant farms one year after this first outbreak was reported in Spain. Further studies are warranted to determine the spatio-temporal trend of SBV in domestic ruminants in this country.J. Cabezas. S. Jiménez-Ruiz holds a PhD contract from the UCLM co-supported by the European Social Fund (2018/12504) and J. Caballero-Gómez is supported by the FPU grant of the Spanish Ministry of Education, Culture and Sport (FPU17/01319).Peer reviewe

Ni tú, ni yo, nosotros

El trabajo obtuvo un Premio Tomás García Verdejo a las buenas prácticas educativas en la Comunidad Autónoma de Extremadura para el curso 2017/2018. Modalidad ASe presenta un proyecto transversal e interdisciplinar llevado a cabo en el Colegio San José (Villanueva de la Serena, Badajoz) que pretendía convertir al colegio en un centro solidario, basado en los 17 objetivos de desarrollo sostenible propuestos por la ONU. Se ha trabajado y experimentado sobre diferentes aspectos, entre ellos: la protección animal, la protección de la infancia, salud, deporte y vida sana, consumo responsable, calentamiento global y energías alternativas e igualdad de géneroExtremaduraES

Laser-driven neutrons for time-of-flight experiments?

Neutron beams, both pulsed and continuous, are a powerful tool in a wide variety of research fields and applications. Nowadays, pulsed neutron beams are produced in conventional accelerator facilities in which the time-of-fight technique is used to determine the kinetic energy of the neutrons inducing the reactions of interest. In the last decades, the development of ultra-short (femtosecond) and ultra-high power (> 1018 W/cm2) lasers has opened the door to a vast number of new applications, including the production and acceleration of pulsed ion beams. These have been recently used to produce pulsed neutron beams, reaching fluxes per pulse similar and even higher than those of conventional neutron beams, hence becoming an alternative for the pulsed neutron beam users community. Nevertheless, these laser-driven neutrons have not been exploited in nuclear physics experiments so far. Our main goal is to produce and characterize laser-driven neutrons but optimizing the analysis, diagnostic and detection techniques currently used in conventional neutron sources to implement them in this new environment. As a result, we would lay down the viability of carrying out nuclear physics experiments using this kind of sources by identifying the advantages and limitations of this production method. To achieve this purpose, we plan to perform experiments in both medium (50TW@L2A2, in Santiago de Com-postela) and high (1PW@APOLLON, in Paris) power laser facilities

Whole-Exome Sequencing of 24 Spanish Families: Candidate Genes for Non-Syndromic Pediatric Keratoconus

Keratoconus is a corneal dystrophy that is one of the main causes of corneal transplantation and for which there is currently no effective treatment for all patients. The presentation of this disease in pediatric age is associated with rapid progression, a worse prognosis and, in 15–20% of cases, the need for corneal transplantation. It is a multifactorial disease with genetic variability, which makes its genetic study difficult. Discovering new therapeutic targets is necessary to improve the quality of life of patients. In this manuscript, we present the results of whole-exome sequencing (WES) of 24 pediatric families diagnosed at the University Hospital La Paz (HULP) in Madrid. The results show an oligogenic inheritance of the disease. Genes involved in the structure, function, cell adhesion, development and repair pathways of the cornea are proposed as candidate genes for the disease. Further studies are needed to confirm the involvement of the candidate genes described in this article in the development of pediatric keratoconus