Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination

Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein, which binds both single-stranded (ss) and double-stranded (ds) DNA and facilitates the formation of higher order protein–DNA complexes in vitro. LrpC binds at different sites within the same DNA molecule promoting intramolecular ligation. When bound to separate molecules, it promotes intermolecular ligation, and joint molecule formation between a circular ssDNA and a homologous ssDNA-tailed linear dsDNA. LrpC binding showed a higher affinity for 4-way (Holliday) junctions in their open conformation, when compared with curved dsDNA. Consistent with these biochemical activities, an lrpC null mutant strain rendered cells sensitive to DNA damaging agents such as methyl methanesulfonate and 4-nitroquinoline-1-oxide, and showed a segregation defect. These findings collectively suggest that LrpC may be involved in DNA transactions during DNA repair and recombination

Influencia de nuevas técnicas de desinfección de suelos sobre la precocidad y productividad del fresón ecológico.

Una pérdida del equilibrio del suelo puede presentar peligrosidad para el cultivo por la presencia de hongos patógenos, nemátodos parásitos, ácaros,…En agricultura ecológica se hace necesaria una rotación de cultivos para controlar la presión de los patógenos, que puede verse complementado con los tratamientos de biofumigación y solarización. Con este trabajo se pretende estudiar la influencia de nuevas técnicas de desinfección de suelos, como son la biofumigación y la solarización, en la precocidad y productividad en el cultivo ecológico de fresón. El ensayo se desarrolló durante las campañas 2003/04 y 2004/05 en una explotación comercial ecológica certificada de Almonte (Huelva). Para ello se diseñó un experimento en bloques al azar, con tres repeticiones en el que se dispusieron tres tratamientos: control (C), biofumigación (BF) y biofumigación+solarización (BF+S). Durante la campaña 2003-04 los resultados mostraron diferencias significativas en la producción de fresón de primera categoría comercial entre los tratamientos biofumigados y el tratamiento control, alcanzándose valores máximos de producción de 623,5 g planta-1 en el tratamiento biofumigación. La precocidad durante la primera campaña mostró diferencias significativas entre el tratamiento biofumigación+solarización y el tratamiento control. En la campaña 2004/05 la influencia de las heladas sobre el cultivo del fresón pudo ser la causa que provocó la ausencia de diferencias significativas entre los tratamientos, a pesar de ello se siguieron obteniendo las mayores producciones con los tratamientos biofumigados

Point of care medical device communication standars (ISO11073/IEEE1073) in patient telemonitoring

Proceeding of: European Medical and Biological Engineering and IFMBE Conference (EMBEC 2005). November 20-25, 2005. Prague, Czech Republic.This paper reviews the use of ISO11073/ IEEE1073 international standard in patient telemonitoring. The purpose of this family of standards is to allow interoperability between medical instrumentation devices and medical information systems. Its application in the field of telemonitoring can encourage telemedicine services and e-care, preventing failures and problems that are making difficult its spread (use problems, high costs of reconfigurations and actualizations). An application guide for the system engineer that want to apply them is proposed, showing the steps to follow, the benefits and handicaps in the standard implementation for different telemonitoring scenarios. The study also includes the conformity levels that have to be fulfilled, the main application points of the standard.This work was supported by projects G03/117 from Fondo de Investigaciones Sanitarias (Spanish Government) and 41/2003 from Departamento de Salud (Navarra Regional Government), and a personal grant to Miguel Galarraga from Departamento de Salud (Navarra Regional Government).No publicad

Stability of class II malocclusion treatment with the austro repositioner followed by fixed appliances in brachyfacial patients

One of the goals of functional-appliance devices is to modify the vertical growth pattern, solving several kinds of malocclusion. This study aimed to evaluate Class II malocclusion treatment’s stability with Austro Repositioner, followed by fixed appliances, and assess its capacity to modify vertical dimensions in brachyfacial patients. A test group of 30 patients (16 boys and 14 girls, mean 11.9 years old) with Class II malocclusion due to mandibular retrognathism and brachyfacial pattern treated with Austro Repositioner and fixed appliance were compared to a matched untreated Class II control group of 30 patients (17 boys and 13 girls, mean age 11.7 years old). Lateral cephalograms were taken at T1 (initial records), T2 (end of treatment), and T3 (one year after treatment). Statistical comparisons were performed with a paired-sample t-test and two-sample t-tests. Significant improvements in the skeletal Class II relationship were observed in the treated group. The ANB angle decreased (4.75°), the SNB angle increased (3.92°), and the total mandibular length (Co-Pg) increased (8.18 mm) (p < 0.001). Vertical dimensions were also significantly modified, the FMA angle increased (3.94°), LAFH-distance increased (3.15 mm), and overbite decreased (3.35 mm). These changes remained stable one year after treatment. The Austro Repositioner was adequate for treating the skeletal Class II malocclusion resulting from the mandible retrusion in brachyfacial patients

Effect of the mother tree age and acorn weight in the regenerative characteristics of Quercus faginea

[EN] The establishment of oak trees is often a slow and difficult process. Hence, it is necessary to determine the characteristics that can lead to improving their regeneration. In this genus, seed size is highly variable both at the interspecific and intraspecific levels, and the effects of intrapopulation variability are not well understood, being even less so for Quercus faginea. In this study, the effects of the age of the mother tree, seed weight and the interaction between these two factors on seed germination, emergence and growth (biomass) were analysed. For this purpose, 16 trees—8 young and 8 old—were selected with the intent to cover the entire range of acorn weights produced in this population. Among the main results, it should be noted that: (1) in older trees, it is easier to find larger acorns; (2) the percentage and the speed of germination of the acorns of young trees is greater than that of old trees; (3) the percentage and the speed of seedling emergence of young trees is greater than that of old trees; and (4) cotyledon weight is the variable that most influences biomass, quite often in a positive way. Therefore, maintaining intrapopulation variability seems to be an approach that most favours the persistence of these populations

Design of an innovative learning experience for the final project of the building engineering degree

This essay presents the bases for a new teaching methodology for the Final Project of the Degree of Building Engineering. The aim of this methodology is to approach students to advanced forms of work in architectural and engineering offices by employing Building Information Modelling (BIM) technologies. This initiative has been funded within the Call 2011 for Teaching Research Incentives ofthe I Teaching Plan of the University of Seville. Following the guidelines of the European Higher Education Area, the learning experience designed has to enable the future Building Engineers to acquire specific and generic competences ascribed to the Final Project in the Verification Report of the Building Engineering Degree. The specific competence “E71. Presentation and defence before a university board of a final project, consisting of an integration exercise of the formative contents received and skills acquired through t he degree” is trained by the development of a building execution project with the use of BIM technologies. For a decade, architecture and engineering offices have increasingly been incorporating in their projects new tools for information processing in digital integrated systems, i.e. programs which allow the construction of building virtual models in three dimensions, and the identification of their constructive components, providing them with parametric dimensions. The operating capacity of BIM programs is stronger than that of 2D drawing programs, since they can manage and generate all the technical documentation in an integrated way. As far as the generic competences are concerned, the problem is that the Final Project has ascribed twenty four competences and their training and evaluation throughout a single term, which seems rather unattainable. In order to solve this matter, the four most important generic competences of Building Engineers have been identified according to their professional profile: “G01. Capacity for organization and planning”, “G06. Information management skills”, “G09. Ability to work in an interdisciplinary team” and “G13. Positive social attitude towards social and technological innovations”. The use of BIM technologies and collaborative work methodologies allow the training of these genericcompetences. Finally, assessment matrixes of the five competences involved have been established with the descriptors of the assessment indicators for each of their corresponding criteria at each level of student achievement. This study is limited to the design of the experience; its implementation could be carried out in the first term of the 2012/2013 academic year, provided the main pre-requisite are met by students, and command of BIM programs such as ALLPLAN, REVIT or ARCHICAD, is achieved. Aware of this challenge− since BIM programs are taught as optional subjects− a curricular line for students interested in participating in this experience is also proposed for the next academic yea

Implementation experience of a patient monitoring solution based on end-to-end standards

Proceeding of: 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS), Cité Internationale, Lyon, France, August 23-26, 2007.This paper presents a proof-of-concept design of a patient monitoring solution for Intensive Care Unit (ICU). It is end-to-end standards-based, using ISO/IEEE 11073 (X73) in the bedside environment and EN13606 to communicate the information to an Electronic Healthcare Record (EHR) server. At the bedside end a plug-and-play sensor network is implemented, which communicates with a gateway that collects the medical information and sends it to a monitoring server. At this point the server transforms the data frame into an EN13606 extract, to be stored on the EHR server. The presented system has been tested in a laboratory environment to demonstrate the feasibility of this end-to-end standardsbased solution.This research work has been partially supported by projects TSI2005-07068-C02-01 and TSI2004-04940-C02-01 from Ministerio de Educación y Ciencia (Spanish Government), and a personal grant to both M.Galarraga and M. Martínez-Espronceda from Navarre Regional Government

Proposal of an ISO/IEEE11073 platform for healthcare telemonitoring: plug-and-play solution with new use cases

Proceeding of: 29th Annual International Conference of IEEE Engineering in Medicine and Biology Society, (EMBS 2007), Lyon, France, 23-26th august 2007.Remote patient monitoring in e-Health is everyday closer to be a mature technology / service. However, there is still a lack of development in areas such as standardization of the sensor’s communication interface, integration into Electronic Healthcare Record systems or incorporation in ambient-intelligent scenarios. This work identifies a set of use cases involved in the personal monitoring scenario and highlights the related features and functionalities, as well as the integration and implementation difficulties found when these are to be implemented in a system based on the ISO/IEEE11073 (X73) standard. It is part of a cooperative research effort devoted to the development of an end-to-end standards-based telemonitoring solution. Standardization committees are working towards adapting the X73 standard to this emerging personal health devices market and use case identification is essential to direct these revisions.This research work has been partially supported by projects TSI2005-07068-C02-01 and TSI2004-04940-C02-01 from Ministerio de Educación y Ciencia (Spanish Government), and a personal grant to both M.Galarraga and M. Martinez-Espronceda from Navarre Regional Governmen

Cognition and the five-factor model of the Positive and Negative Syndrome Scale in schizophrenia

Different exploratory and confirmatory factorial analyses of the Positive and Negative Syndrome Scale (PANSS) have found a number of factors other than the original positive, negative, and general psychopathology. Based on a review of previous studies and using confirmatory factor analyses (CFA), Wallwork et al. (Schizophr Res 2012; 137: 246–250) have recently proposed a consensus five-factor structure of the PANSS. This solution includes a cognitive factor which could be a useful measure of cognition in schizophrenia. Our objectives were 1) to study the psychometric properties (factorial structure and reliability) of this consensus five-factor model of the PANSS, and 2) to study the relationship between executive performance assessed using the Wisconsin Card Sorting Test (WCST) and the proposed PANSS consensus cognitive factor (composed by items P2-N5-G11). This cross-sectional study included a final sample of 201 Spanish outpatients diagnosed with schizophrenia. For our first objective, CFA was performed and Cronbach's alphas of the five factors were calculated; for the second objective, sequential linear regression analyses were used. The results of the CFA showed acceptable fit indices (NNFI = 0.94, CFI = 0.95, RMSEA = 0.08). Cronbach's alphas of the five factors were adequate. Regression analyses showed that this five-factor model of the PANSS explained more of the WCST variance than the classical three-factor model. Moreover, higher cognitive factor scores were associated with worse WCST performance. These results supporting its factorial structure and reliability provide robustness to this consensus PANSS five-factor model, and indicate some usefulness of the cognitive factor in the clinical assessment of schizophrenic patients

Identifying social cognition subgroups in euthymic patients with bipolar disorder: a cluster analytical approach

Background: Bipolar Disorder (BD) is associated with social cognition (SC) impairments even during remission periods although a large heterogeneity has been described. Our aim was to explore the existence of different profiles on SC in patients with BD, and further explore the potential impact of distinct variables on SC. Methods: Hierarchical cluster analysis was conducted using three SC domains (Theory of Mind (ToM), Emotional Intelligence (EI) and Attributional Bias (AB)). The sample comprised of 131 individuals, 71 patients with BD and 60 healthy control subjects who were compared in terms of SC performance, demographic, clinical and neurocognitive variables. A logistic regression model was used to estimate the effect of SC associated risk factors. Results: A two-cluster solution was identified with an adjusted performance group (N=48, 67.6%) and a low performance group (N=23, 32.4%) with mild deficits in ToM and AB domains and with moderate difficulties in EI. Patients with low SC performance were mostly males, showed lower estimated IQ, higher subthreshold depressive symptoms, longer illness duration, and poorer visual memory and attention. Low estimated IQ (OR=0.920; 95%CI=0.863-0.981), male gender (OR=5.661; 95%CI=1.473–21.762) and longer illness duration (OR=1.085; 95%CI=1.006–1.171) contributed the most to the patients clustering. The model explained up to 35% of the variance in SC performance. Conclusions: Our results confirmed the existence of two discrete profiles of SC among BD. Nearly two thirds of patients exhibited adjusted social cognitive abilities. Longer illness duration, male gender and lower estimated IQ were associated with low SC performance.