Antifungal effect and reduction of Ulmus minor symptoms to Ophiostoma novo-ulmi by carvacrol and salicylic acid

There are still no effective means to control Dutch elm disease (DED), caused by the vascular fungi Ophiostoma ulmi and O. novo-ulmi. Plant phenolics may provide a new strategy for DED control, given their known antifungal activity against pathogens and their involvement in plant defence mechanisms. The in vitro antifungal activity of salicylic acid, carvacrol, thymol, phenol, o-cresol, m-cresol, p-cresol, and 2,5-xylenol against the DED pathogens was tested. Also, the protective effect of watering Ulmus minor seedlings with these compounds was tested against O. novo-ulmi. Salicylic acid, carvacrol, and thymol showed the strongest antifungal in vitro activity, while carvacrol and salicylic acid provided the strongest in vivo protection against O. novo-ulmi (63 and 46% reduction of leaf wilting symptoms with respect to controls, respectively). The effect of the treatments on tree phenology was low, and a significant negative relation was observed between the number of days to bud burst and the leaf wilting symptoms after inoculation, probably determined by genetic differences among the elm tree progenies used. The treatments with salicylic acid, carvacrol and thymol induced the highest shift in phenolic metabolite profile with respect to control trees. The protective effect of carvacrol and salicylic acid is discussed in terms of their combined activity as antifungal compounds and as inductors of tree defence responses

Real-Time fusion of visual images and laser data images for safe navigation in outdoor environments

[EN]In recent years, two dimensional laser range finders mounted on vehicles is becoming a fruitful solution to achieve safety and environment recognition requirements (Keicher & Seufert, 2000), (Stentz et al., 2002), (DARPA, 2007). They provide real-time accurate range measurements in large angular fields at a fixed height above the ground plane, and enable robots and vehicles to perform more confidently a variety of tasks by fusing images from visual cameras with range data (Baltzakis et al., 2003). Lasers have normally been used in industrial surveillance applications to detect unexpected objects and persons in indoor environments. In the last decade, laser range finder are moving from indoor to outdoor rural and urban applications for 3D imaging (Yokota et al., 2004), vehicle guidance (Barawid et al., 2007), autonomous navigation (Garcia-Pérez et al., 2008), and objects recognition and classification (Lee & Ehsani, 2008), (Edan & Kondo, 2009), (Katz et al., 2010). Unlike industrial applications, which deal with simple, repetitive and well-defined objects, cameralaser systems on board off-road vehicles require advanced real-time techniques and algorithms to deal with dynamic unexpected objects. Natural environments are complex and loosely structured with great differences among consecutive scenes and scenarios. Vision systems still present severe drawbacks, caused by lighting variability that depends on unpredictable weather conditions. Camera-laser objects feature fusion and classification is still a challenge within the paradigm of artificial perception and mobile robotics in outdoor environments with the presence of dust, dirty, rain, and extreme temperature and humidity. Real time relevant objects perception, task driven, is a main issue for subsequent actions decision in safe unmanned navigation. In comparison with industrial automation systems, the precision required in objects location is usually low, as it is the speed of most rural vehicles that operate in bounded and low structured outdoor environments. To this aim, current work is focused on the development of algorithms and strategies for fusing 2D laser data and visual images, to accomplish real-time detection and classification of unexpected objects close to the vehicle, to guarantee safe navigation. Next, class information can be integrated within the global navigation architecture, in control modules, such as, stop, obstacle avoidance, tracking or mapping.Section 2 includes a description of the commercial vehicle, robot-tractor DEDALO and the vision systems on board. Section 3 addresses some drawbacks in outdoor perception. Section 4 analyses the proposed laser data and visual images fusion method, focused in the reduction of the visual image area to the region of interest wherein objects are detected by the laser. Two methods of segmentation are described in Section 5, to extract the shorter area of the visual image (ROI) resulting from the fusion process. Section 6 displays the colour based classification results of the largest segmented object in the region of interest. Some conclusions are outlined in Section 7, and acknowledgements and references are displayed in Section 8 and Section 9.projects: CICYT- DPI-2006-14497 by the Science and Innovation Ministry, ROBOCITY2030 I y II: Service Robots-PRICIT-CAM-P-DPI-000176- 0505, and SEGVAUTO: Vehicle Safety-PRICIT-CAM-S2009-DPI-1509 by Madrid State Government.Peer reviewe

Firewood and timber exploitation during the third and second millennia BC in Northwestern Iberia: wood resources, territories and chaîne opératoire

Human societies established productive strategies in order to obtain the material resources needed for their day‐to‐day life, including firewood and timber. These strategies were determined by the environmental supply, and also by the cultural characteristics and technical capacities of these communities. This paper presents charcoal analysis data from four Chalcolithic and Bronze Age open‐air settlements located in the northwest of the Iberian Peninsula and occupied during the third and second millennia BC. These results contribute to the knowledge of the exploitation of wooden resources, of the territories where these resources were collected, and of the firewood and timber production process. The identified wood taxa point to a diversified exploitation of the territory, where firewood and timber were collected and/or felled in the deciduous woodland, the river banks and scrubland areas. The characteristics of the wood elements used, the degree of growth ring curvature, the minimum diameter of twigs or logs, etc., as well as other data from archaeological contexts, such as the length of the postholes, were all taken into account to establish hypotheses related to the operative chaine of forest resources. The wooden resources consumed in each site allowed us to hypothesize about the territory of each community.As sociedades humanas estabelecem toda uma série de estratégias produtivas destinadas a obter os meios materiais necessários para a sua existência, entre elas, o aprovisionamento de lenha e de madeira. Estas estratégias, além de estarem condicionadas pela oferta ambiental, são igualmente resultantes das caraterísticas culturais e das capacidades técnicas das comunidades. Os dados arqueobotânicos que se apresentam procedem de vários lugares de habitação do Calcolítico e da Idade do Bronze da fachada ocidental do Noroeste peninsular com cronologias que abarcam o 3º e parte do 2º milénios AC. Os resultados obtidos permitiram identificar uma exploração diversificada dos recursos lenhosos que combinava a recolha de lenha e de madeira da floresta climácica com a proveniente das áreas de mato. As formações arbóreas situadas nas margens dos cursos de água também constituíram fonte de aprovisionamento de combustíveis e, provavelmente, de madeira. A análise dendrológica das amostras registando o grau de curvatura dos anéis de crescimento anual, o diâmetro mínimo dos ramos ou troncos consumidos, entre outras características, em inter‑relação com determinados contextos arqueológicos (dimensões dos buracos de poste, por exemplo), proporcionaram hipóteses relacionadas com a cadeia técnica‑operativa dos recursos florestais. A caracterização dos recursos usados em cada um dos casos de estudo permitiu, igualmente, colocar hipóteses sobre o território de vivência, de circulação e de exploração de cada comunidade.Fundação para a Ciência e Tecnologi

Caracterización anual del metabolismo de las acículas de Pinus pinaster (Aiton) mediante procedimientos transcriptómicos y metabolómicos

El presente trabajo pretende iniciar una serie de estudios sobre el metabolismo nitrogenado en árboles adultos de la conífera Pinus pinaster (Aiton) en condiciones naturales. Durante un año completo se ha caracterizado el metabolismo de las acículas de árboles de P. pinaster que crecen en el paraje natural de Los Reales de Sierra Bermeja, Estepona (Málaga). Para ello se han seguido dos aproximaciones diferentes. En primer lugar se ha analizado la expresión génica mediante el empleo de un microarray para genes de P. pinaster desarrollado en nuestro laboratorio. Por otra parte se ha observado la acumulación de los distintos metabolitos en las acículas a lo largo del año mediante H1-NMR. Para la determinación de los metabolitos presentes en las muestras se ha generado una base de datos de espectros H1-NMR de 90 metabolitos. Para el análisis de los datos se han empleado herramientas estadísticas de clustering. Una de las principales técnicas que se han empleado para el análisis de las muestras ha sido el análisis de las redes de co-expresión de genes (WGCNA). Los resultados de los análisis de los datos sugieren que las acículas de P. pinaster presentan diferentes niveles de actividad a lo largo del año pudiéndose dividir en dos períodos principales, uno ligado al otoño y al invierno y el otro a la primavera y al verano

Biodiesel production from waste cooking oil in an oscillatory flow reactor. Performance as a fuel on a TDI diesel engine

This paper describes the biodiesel production from waste cooking oil (50% (v/v) olive oil/sunflower oil) in an oscillatory flow reactor (OFR) in batch mode. We mainly focused on the characteristics of the biodiesel and its performance as a fuel. First at all, we verified that biodiesel yield in OFR was higher than in stirred tank reactor (STR) under the same experimental conditions, and that composition and properties of the resulting biofuel did not depend on reactor type. Besides, biodiesel production in OFR took half the time than in STR. Subsequently, we modify some OFR operational parameters to assess their influence on biodiesel yield. The most suitable conditions were found to be 6:1 methanol to waste cooking oil molar ratio, 0.67 Hz oscillation frequency and 30 min reaction time. Finally, the biofuel obtained was tested in a 2.0 TDI 140 hp EURO4 engine installed on an engine test bench. Specific fuel consumption, particle size distribution and concentration of exhaust gas sample pollutants and were analysed running with commercial diesel, 50% (v/v) diesel/biodiesel blend (B50) and biodiesel (B100) in order to ensure the viability of using this biofuel in vehicle engines.Fondos de la Unión Europea - LIFE 13-Bioseville ENV/111

The complete nucleotide sequence of a Spanish isolate of <i>Citrus psorosis</i> virus: comparative analysis with other ophioviruses

The complete genomic sequence (11278 nt) of Citrus psorosis virus (CPsV), isolate P-121 from Spain, was determined and compared with those from isolate CPV-4 and from other ophioviruses. The three RNAs of P-121 had similar size and identical organization as those of CPV-4. The 24K and the RdRp proteins were potentially encoded in the viral complementary (vc) strand of RNA 1, the 54K protein potentially encoded in vcRNA 2 and the coat protein encoded in vcRNA 3. These four proteins from P-121 and CPV-4 had 87, 92, 93 and 94% amino acid identity, respectively, but only 22, 38, 25 and 33% identity with their homologous proteins from Mirafiori lettuce big vein virus (MLBVV), the only other ophiovirus completely sequenced. Biological and genetic differences between CPsV and MLBVV (and the other ophioviruses), would support their future allocation in different genera within a tentative family Ophioviridae.Instituto de Biotecnología y Biología Molecula

Impact of sensor-augmented pump therapy with predictive low-glucose management on hypoglycemia and glycemic control in patients with type 1 diabetes mellitus : 1-year follow-up

Q2Artículo original2625-2631AIMS: To describe real-life experience with sensor-augmented pump therapy with predictive low-glucose management (SAPT-PLGM), in terms of hypoglycemia and glycemic control after one year of follow-up in T1D patients with hypoglycemia as the main indication of therapy. METHODS: Retrospective cohort study under real life conditions. Baseline and one-year follow-up variables of glycemic control, hypoglycemia and glycemic variability were compared. RESULTS: Fifty patients were included, 31 on prior treatment with SAPT with low-glucose suspend (LGS) feature and 19 on multiple dose insulin injections (MDI). Mean HbA1c decreased in the MDI group (8.24%-7.08%; p = 0.0001). HbA1c change was not significant in the SAPT-LGS group. Area under the curve (AUC) below 70 mg/dl improved in both SAPT-LGS and MDI groups while AUC, %time and events below 54 mg/dl decreased in SAPT-LGS group. Glycemic variability improved in the MDI group. Less patients presented severe hypoglycemia with SAPT-PLGM in both groups, however the change was non-significant. CONCLUSIONS: Under real life conditions, SAPT-PLGM reduced metrics of hypoglycemia in patients previously treaded with MDI and SAPT-LGS without deteriorating glycemic control in SAPT-LGS patients, while improving it in patients treated with MDI

Targeting of prolamins by RNAi in bread wheat: Effectiveness of seven silencing-fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins

Gluten proteins are responsible for the viscoelastic properties of wheat flour but also for triggering pathologies in susceptible individuals, of which coeliac disease (CD) and noncoeliac gluten sensitivity may affect up to 8% of the population. The only effective treatment for affected persons is a strict gluten-free diet. Here, we report the effectiveness of seven plasmid combinations, encompassing RNAi fragments from a-, c-, x-gliadins, and LMW glutenin subunits, for silencing the expression of different prolamin fractions. Silencing patterns of transgenic lines were analysed by gel electrophoresis, RP-HPLC and mass spectrometry (LC-MS/ MS), whereas gluten immunogenicity was assayed by an anti-gliadin 33-mer monoclonal antibody (moAb). Plasmid combinations 1 and 2 downregulated only c- and a-gliadins, respectively. Four plasmid combinations were highly effective in the silencing of x-gliadins and c-gliadins, and three of these also silenced a-gliadins. HMW glutenins were upregulated in all but one plasmid combination, while LMW glutenins were downregulated in three plasmid combinations. Total protein and starch contents were unaffected regardless of the plasmid combination used. Six plasmid combinations provided strong reduction in the gluten content as measured by moAb and for two combinations, this reduction was higher than 90% in comparison with the wild type. CD epitope analysis in peptides identified in LC-MS/MS showed that lines from three plasmid combinations were totally devoid of CD epitopes from the highly immunogenic a- and x-gliadins. Our findings raise the prospect of breeding wheat species with low levels of harmful gluten, and of achieving the important goal of developing nontoxic wheat cultivars

The informational role of thin options markets: Empirical evidence from the Spanish case

This study investigates the informational role of thin options markets, specifically the Spanish options market. Firstly, we examine the effect of options markets by analysing stock market reaction to earnings news, conditional on the availability of options markets. Secondly, we examine options trading activity before the release of earnings news (including the announcement period). The results show that the impact on prices before the earnings release is significantly bigger when options trading is available. Moreover, the dissemination of earnings news is associated with significant unusual activity in the options market due to informed trading, especially when the earnings surprise is highly good

Prototype of a high resolution regional reanalysis for Iberian Peninsula an Balearic Islands (IBERA)

Póster presentado en: 1st ACCORD All Staff Workshop celebrado del 12 al 16 de abril de 2021 de forma online