Loco afán: imaginario travesti

This article explains how Pedro Lemebel creates a transvestite imagery in his body of work, particularly in Loco afán, through a social and cultural vision within the artistic field and the sexual-generic frame. He values subversion and new ways of expression in the post dictatorial Chile. Because it comes from marginality, his writing generates new ideas and strategies to question the normalization and categorization that is imposed on homosexuality. Loco afán contains some of the stories of Lemebel’s friends, who suffered due to AIDS, discrimination, prostitution and poberty. I will analize two of them: “El último beso de Loba Lamar” and “La muerte de Madonna”, studying the main characters and their struggles. The author gives these characters life beyond death, allowing their voices to be heard after they where silenced by the military dictatorship and AIDS.Este artículo explica cómo Pedro Lemebel construye un imaginario travesti en sus escritos, particularmente en Loco afán, mediante una visión social y cultural que valora la diferencia, lo subversivo y las nuevas formas de expresión en el Chile post dictadura, tanto en lo genérico-sexual como en lo artístico. Al provenir de la marginalidad, su escritura genera nuevas ideas y estrategias para cuestionar la normalización y la categorización que se le impone a la homosexualidad. Loco afán contiene algunas de las historias de las amigas de Lemebel, quienes sufrieron por el SIDA, la discriminación, la prostitución y la pobreza. Se analizarán dos de ellas: “El último beso de Loba Lamar” y “La muerte de Madonna”, estudiando a los personajes principales y los desafíos que enfrentan. El autor da vida a los protagonistas más allá de la muerte, permitiendo escuchar sus voces que fueron silenciadas por la dictadura militar y por el SIDA

The Land of Elyon: Books 1 & 2

This poster provides context for the children\u27s fantasy series The Land of Elyon through maps and a diagram of the fictional world.https://digitalcommons.brockport.edu/graphic_design_posters/1007/thumbnail.jp

Beach Hut Deli Case Study

Beach Hut Deli Case Study describes the origin, development and growth of a quick service restaurant in Granite Bay, CA, which has now expanded to 35 locations in California, Reno, and Las Vegas with plans to expand into the Pacific Northwest. The case discusses in-house and external marketing, menu development and franchising options and issues

Strategies for an urban renewal in Rome: Massimina Co_Goal

Abstract The paper presents a research project about the regeneration of an informal neighbourhood in Rome. To address the complexity of the regeneration process, the project relies on a combined top down/bottom up strategy. The project envisions the implementation of a Public Call to promote a set of retrofitting actions for a defined number of private houses to be involved in a co-financed refurbishment program. Main goal of the Public Call is to foster the transition towards sustainable development, transforming the existing district into a low energy district and developing new community services implemented and managed by a local community company

Glove Port Technique for Transanal Endoscopic Microsurgery

Introduction. Despite initial enthusiasm, the use of transanal endoscopic microsurgery (TEM) is still quite limited at present because of the expense of highly specialized equipment and the complexity of the learning curve. Furthermore, some authors report a relevant, although temporary, effect on anorectal function because of the considerable anal dilatation which can even produce a rupture of the internal anal sphincter. The “glove TEM” proposes itself as an alternative to traditional TEM that could settle these problems. Materials and Methods. The technique is accurately described together with the necessary equipment to perform it. Between 2011 and 2012, we operated eight patients with this technique for rectal adenomas or early carcinomas achieving R0 resection in all cases and reporting no early or late complications during the first five months of followup. Discussion. This technique offers multiple advantages compared to the original TEM. (i) It allows the use of all available laparoscopic instruments. (ii) It gives a great manoeuvrability of the instruments in contrast to rigid rectoscope systems. (iii) Given the limited length of the device, it permits to operate on tumors closer to the dentate line. (iv) It is less traumatic to the anal sphincter. It is definitively much cheaper. Conclusions. We believe that this new technique is easy to perform, cost-effective, and less traumatic to the anal sphincter compared to traditional TEM

A Drosophila protein-interaction map centered on cell-cycle regulators

BACKGROUND: Maps depicting binary interactions between proteins can be powerful starting points for understanding biological systems. A proven technology for generating such maps is high-throughput yeast two-hybrid screening. In the most extensive screen to date, a Gal4-based two-hybrid system was used recently to detect over 20,000 interactions among Drosophila proteins. Although these data are a valuable resource for insights into protein networks, they cover only a fraction of the expected number of interactions. RESULTS: To complement the Gal4-based interaction data, we used the same set of Drosophila open reading frames to construct arrays for a LexA-based two-hybrid system. We screened the arrays using a novel pooled mating approach, initially focusing on proteins related to cell-cycle regulators. We detected 1,814 reproducible interactions among 488 proteins. The map includes a large number of novel interactions with potential biological significance. Informative regions of the map could be highlighted by searching for paralogous interactions and by clustering proteins on the basis of their interaction profiles. Surprisingly, only 28 interactions were found in common between the LexA- and Gal4-based screens, even though they had similar rates of true positives. CONCLUSIONS: The substantial number of new interactions discovered here supports the conclusion that previous interaction mapping studies were far from complete and that many more interactions remain to be found. Our results indicate that different two-hybrid systems and screening approaches applied to the same proteome can generate more comprehensive datasets with more cross-validated interactions. The cell-cycle map provides a guide for further defining important regulatory networks in Drosophila and other organisms

PDGFRα depletion attenuates glioblastoma stem cells features by modulation of STAT3, RB1 and multiple oncogenic signals.

Platelet derived growth factor receptors (PDGFRs) play an important role in tumor pathogenesis, and they are frequently overexpressed in glioblastoma (GBM). Earlier we have shown a higher protein expression of PDGFR isoforms (α and β) in peritumoral-tissue derived cancer stem cells (p-CSC) than in tumor core (c-CSC) of several GBM affected patients. In the current study, in order to assess the activity of PDGFRα/PDGF-AA signaling axis, we performed time course experiments to monitor the effects of exogenous PDGF-AA on the expression of downstream target genes in c-CSC vs p-CSC. Interestingly, in p-CSC we detected the upregulation of Y705-phosphorylated Stat3, concurrent with a decrement of Rb1 protein in its active state, within minutes of PDGF-AA addition. This finding prompted us to elucidate the role of PDGFRα in self-renewal, invasion and differentiation in p-CSC by using short hairpin RNA depletion of PDGFRα expression. Notably, in PDGFRα-depleted cells, protein analysis revealed attenuation of stemness-related and glial markers expression, alongside early activation of the neuronal marker MAP2a/b that correlated with the induction of tumor suppressor Rb1. The in vitro reduction of the invasive capacity of PDGFRα-depleted CSC as compared to parental cells correlated with the downmodulation of markers of epithelial-mesenchymal transition phenotype and angiogenesis. Surprisingly, we observed the induction of anti-apoptotic proteins and compensatory oncogenic signals such as EDN1, EDNRB, PRKCB1, PDGF-C and PDGF-D. To conclude, we hypothesize that the newly discovered PDGFRα/Stat3/Rb1 regulatory axis might represent a potential therapeutic target for GBM treatment.BRC, Q

Ultrasound tissue characterization detectspreclinical myocardial structural changes inchildren affected by Duchenne muscular dystrophy

AbstractObjectivesOur goal was to identify early changes in myocardial physical properties in children with Duchenne muscular dystrophy (DMDch).BackgroundDuchenne muscular dystrophy (DMD) is caused by the absence of dystrophin, which triggers complex molecular and biological events in skeletal and cardiac muscle tissues. Although about 30% of patients display overt signs of cardiomyopathy in the late stage of the disease, it is unknown whether changes in myocardial physical properties can be detected in the early (preclinical) stages of the disease.MethodsWe performed an ultrasonic tissue characterization (UTC) analysis of myocardium in DMDch with normal systolic myocardial function and no signs of cardiomyopathy. Both the cyclic variation of integrated backscatter (cvIBS) and the calibrated integrated backscatter (cIBS) were assessed in 8 myocardial regions of 20 DMDch, age 7 ± 2 years (range 4 to 10 years), and in 20 age-matched healthy controls.ResultsWe found large differences in the UTC data between DMDch and controls; the mean value of cvIBS was 4.4 ± 1.5 dB versus 8.8 ± 0.8 dB, whereas the mean value of cIBS was 36.4 ± 7.1 dB versus 26.9 ± 2.0 dB (p < 10−6for both). In DMDch, all eight sampled segments showed cIBS mean values to be significantly higher and cvIBS mean values to be significantly lower than those in the controls. Finally, interindividual differences were greater in DMDch than in controls for both parameters.ConclusionsThe myocardium in DMDch displays UTC features different from those in healthy controls. These results show that lack of dystrophin is commonly associated with changes in myocardial features well before the onset of changes of systolic function and overt cardiomyopathy