Editorial: Environmental phytoremediation: plants and microorganisms at work

Human industry, farming, and waste disposal practices have resulted in the large-scale contamination of soil and water with organic compounds and heavy metals, with detrimental effects on ecosystems and human health. Conventional soil remediation methods are expensiveand often involve the storage of soil in designated areas, postponing rather than solving the problem. In the last decade, the pressing need to find alternative methods has highlighted the scientific and economic benefits of plants and their associated microorganisms, which can beused for the reclamation of polluted soil and water (Meagher, 2000). This is an elegant and low-cost approach for the decontamination of polluted sites and has been greeted with a high degree of public acceptance, therefore prompting research into the use of phytoremediation technology to address the large areas of land and water currently affected (reviewed by Kr\ue4mer,2005; Vangronsveld et al., 2009; Lee, 2013). This Frontiers in Plant Science research topic provides a snapshot of current research into the application of environmental phytoremediation strategies

Stellar masses and disk properties of Lupus young stellar objects traced by velocity-aligned stacked ALMA 13CO and C18O spectra

In recent ALMA surveys, the gas distributions and velocity structures of most of the protoplanetary disks can still not be imaged at high S/N due to the short integration time. In this work, we re-analyzed the ALMA 13CO (3-2) and C18O (3-2) data of 88 young stellar objects in Lupus with the velocity-aligned stacking method to enhance S/N and to study the kinematics and disk properties traced by molecular lines. This method aligns spectra at different positions in a disk based on the projected Keplerian velocities at their positions and then stacks them. This method enhances the S/N ratios of molecular-line data and allows us to obtain better detections and to constrain dynamical stellar masses and disk orientations. We obtain 13CO detections in 41 disks and C18O detections in 18 disks with 11 new detections in 13CO and 9 new detections in C18O after applying the method. We estimate the disk orientations and the dynamical stellar masses from the 13CO data. Our estimated dynamical stellar masses correlate with the spectroscopic stellar masses, and in a subsample of 16 sources, where the inclination angles are better constrained, the two masses are in a good agreement within the uncertainties and with a mean difference of 0.15 Msun. With more detections of fainter disks, our results show that high gas masses derived from the 13CO and C18O lines tend to be associated with high dust masses estimated from the continuum emission. Nevertheless, the scatter is large (0.9 dex), implying large uncertainties in deriving the disk gas mass from the line fluxes. We find that with such large uncertainties it is expected that there is no correlation between the disk gas mass and the mass accretion rate with the current data. Deeper observations to detect disks with gas masses <1E-5 Msun in molecular lines are needed to investigate the correlation between the disk gas mass and the mass accretion rate.Comment: Submitted to A&

The Role of the Atypical Kinases ABC1K7 and ABC1K8 in Abscisic Acid Responses

The ABC1K family of atypical kinases (activity of bc1 complex kinase) is represented in bacteria, archaea, and eukaryotes. In plants they regulate diverse physiological processes in the chloroplasts and mitochondria, but their precise functions are poorly defined. ABC1K7 and ABC1K8 are probably involved in oxidative stress responses, isoprenyl lipid synthesis and distribution of iron within chloroplasts. Because reactive oxygen species take part in abscisic acid (ABA)-mediated processes, we investigated the functions of ABC1K7 and ABC1K8 during germination, stomatal movement, and leaf senescence. Both genes were upregulated by ABA treatment and some ABA-responsive physiological processes were affected in abc1k7 and abc1k8 mutants. Germination was more severely affected by ABA, osmotic stress and salt stress in the single and double mutants; the stomatal aperture was smaller in the mutants under standard growth conditions and was not further reduced by exogenous ABA application; ABA-induced senescence symptoms were more severe in the leaves of the single and double mutants compared to wild type leaves. Taken together, our results suggest that ABC1K7 and ABC1K8 might be involved in the cross-talk between ABA and ROS signaling

Evolution of the metal hyperaccumulation and hypertolerance traits

In this review, we overview the most updated concepts regarding the evolution of hyperaccumulation and hypertolerance, highlighting also the ecological context concerning the plant populations displaying this fascinating phenomenon

Plants expressing murine pro-apoptotic protein Bid do not have enhanced PCD

The purpose of this study was to explore whether plant programmed cell death (PCD) cascade can sense the presence of the animal-only BH3 protein Bid, a BCL-2 family protein known to play a regulatory role in the signaling cascade of animal apoptosis

An ALMA Survey of Protoplanetary Disks in the σ\sigma Orionis Cluster

The $\sigma$ Orionis cluster is important for studying protoplanetary disk evolution, as its intermediate age ($\sim$3-5 Myr) is comparable to the median disk lifetime. We use ALMA to conduct a high-sensitivity survey of dust and gas in 92 protoplanetary disks around $\sigma$ Orionis members with $M_{\ast}\gtrsim0.1 M_{\odot}$. Our observations cover the 1.33 mm continuum and several CO $J=2-1$ lines: out of 92 sources, we detect 37 in the mm continuum and six in $^{12}$CO, three in $^{13}$CO, and none in C$^{18}$O. Using the continuum emission to estimate dust mass, we find only 11 disks with $M_{\rm dust}\gtrsim10 M_{\oplus}$, indicating that after only a few Myr of evolution most disks lack sufficient dust to form giant planet cores. Stacking the individually undetected continuum sources limits their average dust mass to 5$\times$ lower than that of the faintest detected disk, supporting theoretical models that indicate rapid dissipation once disk clearing begins. Comparing the protoplanetary disk population in $\sigma$ Orionis to those of other star-forming regions supports the steady decline in average dust mass and the steepening of the $M_{\rm dust}$-$M_{\ast}$ relation with age; studying these evolutionary trends can inform the relative importance of different disk processes during key eras of planet formation. External photoevaporation from the central O9 star is influencing disk evolution throughout the region: dust masses clearly decline with decreasing separation from the photoionizing source, and the handful of CO detections exist at projected separations $>1.5$ pc. Collectively, our findings indicate that giant planet formation is inherently rare and/or well underway by a few Myr of age.Comment: 16 pages, 9 figures; published in AJ; The full machine readable tables can be obtained by downloading and extracting the gzipped tar source file listed under "Other formats.

Probing UV-sensitive Pathways for CN and HCN Formation in Protoplanetary Disks with the Hubble Space Telescope

The UV radiation field is a critical regulator of gas-phase chemistry in surface layers of disks around young stars. In an effort to understand the relationship between photocatalyzing UV radiation fields and gas emission observed at infrared and submillimeter wavelengths, we present an analysis of new and archival Hubble Space Telescope (HST), Spitzer, ALMA, IRAM, and SMA data for five targets in the Lupus cloud complex and 14 systems in Taurus-Auriga. The HST spectra were used to measure Lyα and far-UV (FUV) continuum fluxes reaching the disk surface, which are responsible for dissociating relevant molecular species (e.g., HCN, N₂). Semi-forbidden C II] λ2325 and UV-fluorescent H₂ emission were also measured to constrain inner disk populations of C⁺ and vibrationally excited H2. We find a significant positive correlation between 14 μm HCN emission and fluxes from the FUV continuum and C II] λ2325, consistent with model predictions requiring N₂ photodissociation and carbon ionization to trigger the main CN/HCN formation pathways. We also report significant negative correlations between submillimeter CN emission and both C II] and FUV continuum fluxes, implying that CN is also more readily dissociated in disks with stronger FUV irradiation. No clear relationships are detected between either CN or HCN and Lyα or UV-H₂ emission. This is attributed to the spatial stratification of the various molecular species, which span several vertical layers and radii across the inner and outer disk. We expect that future observations with the James Webb Space Telescope will build on this work by enabling more sensitive IR surveys than were possible with Spitzer

Enhancement of Zn tolerance and accumulation in plants mediated by the expression of Saccharomyces cerevisiae vacuolar transporter ZRC1

Main conclusion Transgenic Arabidopsis thaliana and Populus alba plants overexpressing the zinc transporter ScZRC1 in shoots exhibit Zn tolerance. Increased Zn concentrations were observed in shoots of P. alba, a species suitable for phytoremediation. Genetic engineering of plants for phytoremediation is worth to consider if genes leading to heavy metal accumulation and tolerance are expressed in high biomass producing plants. The Saccharomyces cerevisiae ZRC1 gene encodes a zinc transporter which is primarily involved in the uptake of Zn into the vacuole. The ZRC1 gene was expressed in the model species A. thaliana and P. alba (cv. Villafranca). Both species were transformed with constructs carrying ScZRC1 under the control of either the CaMV35S promoter for constitutive expression or the active promoter region of the tobacco Rubisco small subunit (pRbcS) to limit the expression to the above-ground tissues. In hydroponic cultures, A. thaliana and poplar ScZRC1-expressing plants accumulated more Zn in vegetative tissues and were more tolerant than untransformed plants. No differences were found between plants carrying the CaMV35::ScZRC1 or pRbcS::ScZRC1 constructs. The higher Zn accumulation in transgenic plants was accompanied by an increased superoxide dismutase (SOD) activity, indicating the activation of defense mechanisms to prevent cellular damage. In the presence of cadmium in addition to Zn, plants did not show symptoms of metal toxicity, neither in hydroponic cultures nor in soil. Zn accumulation increased in shoots, while no differences were observed for Cd accumulation, in comparison to control plants. These data suggest that ectopic expression of ScZRC1 can increase the potential of poplar for the remediation of Zn-polluted soils, although further tests are required to assay its application in remediating multimetal polluted soils

New insights into the nature of transition disks from a complete disk survey of the Lupus star forming region

Transition disks with large dust cavities around young stars are promising targets for studying planet formation. Previous studies have revealed the presence of gas cavities inside the dust cavities hinting at recently formed, giant planets. However, many of these studies are biased towards the brightest disks in the nearby star forming regions, and it is not possible to derive reliable statistics that can be compared with exoplanet populations. We present the analysis of 11 transition disks with large cavities (>20 AU radius) from a complete disk survey of the Lupus star forming region, using ALMA Band 7 observations at 0.3" (22-30 AU radius) resolution of the 345 GHz continuum, 13CO and C18O 3-2 observations and the Spectral Energy Distribution of each source. Gas and dust surface density profiles are derived using the physical-chemical modeling code DALI. This is the first study of transition disks of large cavities within a complete disk survey within a star forming region. The dust cavity sizes range from 20-90 AU radius and in three cases, a gas cavity is resolved as well. The deep drops in gas density and large dust cavity sizes are consistent with clearing by giant planets. The fraction of transition disks with large cavities in Lupus is ~11%, which is inconsistent with exoplanet population studies of giant planets at wide orbits. Furthermore, we present a hypothesis of an evolutionary path for large massive disks evolving into transition disks with large cavities.Comment: 29 pages, 15 figures, Accepted by Ap

disrupting the pcsk9 ldlr protein protein interaction by an imidazole based minimalist peptidomimetic

We report on a tetraimidazole-based β-strand minimalist peptidomimetic as a novel inhibitor of LDLR–PCSK9 protein–protein interaction, a promising target for hypercholesterolemia