Induction and recovery times during immersion anaesthesia with metacaine and benzocaine of medium sized fish (200–400 g).

<p>Times for induction and recovery with different doses of (A) metacaine at 6°C, (B) benzocaine at 6°C, (C) metacaine at 12°C, (D) benzocaine at 12°C. The colored boxes show times for induction and recovery. Average time in minutes ± S.D. is shown. Red boxes = induction times, black boxes = initial respiration, blue boxes = normal respiration, grey boxes = swimming activity. Single letters means that this measurement is statistically different the other. Shared letters means that the differences are not statistically significant (P < 0.05). For all anaesthetic concentrations N = 10, except for metacaine 1600 mg L^-1 at 6°C and benzocaine 800 mg L^-1 6°C for which N = 5. Statistical analyses for induction and each of the phases during recovery, as well as statistical analyses of the effect of temperature, are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#pone.0179344.s004" target="_blank">S4</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#pone.0179344.s007" target="_blank">S7</a> Tables.</p

Induction and recovery times during immersion anaesthesia with metacaine, benzocaine and isoeugenol of small fish (10–20 g).

<p>Induction and recovery with different doses of (A) metacaine at 6°C, (B) benzocaine at 6°C, (C) isoeugenol at 6°C, (D) metacaine at 12°C, (E) benzocaine at 12°C, (F) isoeugenol at 12°C. The colored boxes show times for induction and recovery. Average time in minutes ± S.D. is shown. Red boxes = induction times (no respiration), black boxes = initial respiration, blue boxes = normal respiration, grey boxes = swimming activity. Single letters means that this measurement is statistically different the other. Shared letters means that the differences are not statistically significant (P < 0.05). N = 10 for all anaesthetic concentrations, except for isoeugenol at 6°C for which N = 5. Statistical analyses for induction and each of the phases during recovery, as well as statistical analyses of the effect of temperature, are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#pone.0179344.s004" target="_blank">S4</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#pone.0179344.s007" target="_blank">S7</a> Tables.</p

Induction and recovery times during immersion anaesthesia with metacaine of large fish (600–1300 g).

<p>Induction and recovery times with different doses of (A) metacaine at 6°C, (B) metacaine at 12°C. The colored boxes show times for induction and recovery. Average time in minutes ± S.D. is shown. Red boxes = induction times, black boxes = initial respiration, blue boxes = normal respiration, grey boxes = swimming activity. Single letters means that this measurement is statistically different the other. Shared letters means that the differences are not statistically significant (P < 0.05). N = 5. Statistical analyses for induction and each of the phases during recovery, as well as statistical analyses of the effect of temperature, are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#pone.0179344.s004" target="_blank">S4</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#pone.0179344.s007" target="_blank">S7</a> Tables.</p

Overview of recommended concentrations for full anaesthesia of lumpfish.

<p>Overview of recommended concentrations for full anaesthesia of lumpfish.</p

Overview of behaviour of lumpfish at different stages of anaesthesia ¹.

<p>Overview of behaviour of lumpfish at different stages of anaesthesia <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179344#t001fn001" target="_blank">¹</a>.</p

Diplotaxis glabrimargo (dorsal)

Paratipo de Diplotaxis glabrimargo Vaurie y Cazie