Antibacterial activity of Nigella sativa seed extracts

The development and alarming increase of bacterial resistance to existing antimicrobial agents has become a real challenge and a serious problem facing patients suffering from various infections worldwide. This study was designed to evaluate the antibacterial activity of black seed extracts against bacterial isolates (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa). The powdered seeds were extracted using percolation technique with methanol and petroleum ether. Agar well diffusion method was used to test the antibacterial activity. Methanolic extracts at 100mg/ml had a remarkable activity against S. aureus (19mm) and P. aeruginosa (12mm). Also the petroleum ether extract had the same activity against S. aureus and P. aeruginosa (10mm). This implies that methanolic and petroleum ether extracts of N. sativa were found to be active against S. aureus and P. aeruginosa. While E. coli showed resistance to the extracts at all concentrations. Our study shows that species, strains and concentrations of N. sativa extracts are some of the factors that may influence the sensitivity of tested organisms.Keywords: Antibacterial activity, Nigella sativa, Black seed extract, Agar well diffusion, Bacterial isolate

Differential grain yield performance of single cross hybrid maize in varied nitrogen conditions

Presence of genotypic differences in maize ( Zea mays L.) grain yield in multi-environments would help plant breeders to make logical decisions in improvement programmes of the crop. Hence, the grain yield performance, heterosis and stability in hybrid maize in varied soil nitrogen (N) conditions in Nigeria were assessed using multiple biometrical techniques. Grain yield performance, heterosis and stability of 150 single cross hybrid maize were assessed with checks in severe N stress, N stress and optimal conditions in a replicated trial laid out in 19 7 8 lattice design for 2 years. The grain yield was subjected to analysis of variance for each N condition and combined across years. Heterosis was estimated for the trait, additive mean effects and multiplicative interaction (AMMI); and genotype + genotype 7 environment (GGE) models were used to analyse the hybrids\u2019 yield stability. The AMMI captured 93.7%; while GGE bi-plot accounted for 88.7% of total variation among the hybrids. Eight hybrids had high yields across the N conditions. Genotypes and environments affected heterosis for grain yield. Hybrids TZEI7 7BD74-399, BD74-179 7BD74-55 and BD74-175 7BD74-147 were the most ideal genotypes for the N conditions; while severe N stress condition was ideal test environment. Hybrid BD74-170 7BD74-31 adapted most to N stress; while TZEI1 7BD74-399 adapted most to severe N stress and to optimal conditions. Based on yield, heterosis and stability, hybrids involving inbred line BD74-171 were recommended for N stress; while those with inbred lines TZEI1, TZEI4 BD74-170, BD74-128, BD74-179 and BD74-175 were adapted to N stress and optimal conditions.La pr\ue9sence de diff\ue9rences g\ue9notypiques dans le rendement en grains de ma\uefs ( Zea mays L.) dans plusieurs environnements aiderait les selectioneurs \ue0 prendre des d\ue9cisions logiques dans les programmes d\u2019am\ue9lioration de ma\uefs. Par cons\ue9quent, les performances de rendement en grain, l\u2019h\ue9t\ue9rosis et la stabilit\ue9 d\u2018 hybride de ma\uefs dans les diverses conditions d\u2019azote (N) du sol au Nig\ue9ria ont \ue9t\ue9 \ue9valu\ue9es \ue0 l\u2019aide de multiples techniques biom\ue9triques. La performance de rendement en grain, l\u2019h\ue9t\ue9rosis et la stabilit\ue9 de 150 des hybrides de ma\uefs simples crois\ue9s ont \ue9t\ue9 \ue9valu\ue9s avec des contr\uf4les du stress grave en N, du stress en N et des conditions optimales dans le cadre d\u2019un essai r\ue9p\ue9t\ue9 pr\ue9sent\ue9 sur un r\ue9seau 19 7 8 pendant 2 ans. Le rendement en grains a \ue9t\ue9 soumis \ue0 une analyse de variance pour chaque condition d\u2019azote et combin\ue9 sur plusieurs ann\ue9es. L\u2019h\ue9t\ue9rosis a \ue9t\ue9 estim\ue9e pour le trait, les effets moyens additifs et l\u2019interaction multiplicative (MAIM); et des mod\ue8les g\ue9notype + g\ue9notype 7 environnement (GGE) ont \ue9t\ue9 utilis\ue9s pour analyser la stabilit\ue9 du rendement des hybrides. Le MAIM a captur\ue9 93,7%; alors que les bi-parcelles GGE ont repr\ue9sent\ue9 88,7% de la variation totale parmi les hybrides. Huit hybrides ont eu des rendements \ue9lev\ue9s dans les conditions d\u2019azote (N) du sol. Les g\ue9notypes et les environnements ont affect\ue9 l\u2019h\ue9t\ue9rosis pour le rendement en grain. Les hybrides TZEI7 7 BD74-399, BD74-179 7 BD74-55 et BD74-175 7 BD74-147 \ue9taient les g\ue9notypes les plus id\ue9aux pour les conditions N; tandis que la condition de stress N grave \ue9tait l\u2019environnement de test id\ue9al. Les hybrides BD74-170 7 BD74-31 \ue9taient les mieux adapt\ue9s au stress N; TZEI1 7 BD74-399 \ue9taient plus adapt\ue9s \ue0 l\u2018 intense stress li\ue9 \ue0 l\u2019azote et aux conditions optimales. En se basant sur le rendement, l\u2019h\ue9t\ue9rosis et la stabilit\ue9, des hybrides impliquant la lign\ue9e consanguine BD74-171 ont \ue9t\ue9 recommand\ue9s pour le stress sous N; tandis que ceux avec des lign\ue9es consanguines TZEI1, TZEI4 BD74-170, BD74-128, BD74-179 et BD74-175 ont \ue9t\ue9 adapt\ue9s au stress N et \ue0 des conditions optimales

Comparative assessment of brain and circulating oxidative stress biomarkers in weaned New Zealand White rabbits supplemented with microalga Chlorella vulgaris biomass

The brain is central to human and animal well-being but it requires a high amount of oxygen for its normal functioning and this makes it an organ highly vulnerable to oxidative stress damage. Therefore, for the promotion of normal physiological and cellular functions of the brain, antioxidant intake is very critical. This study investigated the antioxidant enzymatic activities in the brain by measuring activities of superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in connection with the circulating oxidative stress biomarkers of the serum and liver of New Zealand White rabbits supplemented with microalga Chlorella vulgaris biomass in addition to regular basal diets. The study involved a random distribution of 40 rabbits of eight weeks old into five experimental group using completely randomized design. The rabbits were observed for a period of 120 when they are being supplemented after which their blood, brain, and liver were collected for analyses. The results show that the total antioxidant capacity was higher in the brain of the supplemented rabbits (P<0.05). Although, there was no significant difference in the brain malondialdehyde concentrations, there were higher activities of antioxidant enzymes in the brain of the supplemented rabbits (P< 0.05). There was a lower concentration of the circulating malondialdehyde (MDA) in the serum and liver of the supplemented rabbits. The study concluded that Chlorella vulgaris intake led to reduced circulating malondialdehyde and increased activities of the brain antioxidant enzymes in the rabbits. The study indicated that the microalga Chlorella vulgaris contains antioxidant compounds that can cross the blood-brain barrier, which could be a very important therapeutic agent against oxidative stress-induced brain complications in animals and humans

Combining ability of extra-early maturing provitamin A maize (Zea mays L.) inbred lines and performance of derived hybrids under Striga hermonthica infestation and low soil nitrogen

Open Access JournalLow soil nitrogen (low-N), Striga hermonthica infestation and vitamin A deficiency in normal endosperm maize are major challenges confronting maize production and nutrition of the people of sub-Saharan Africa (SSA). Development of pro-vitamin A (PVA) maize hybrids with combined resistance/tolerance to the two stress factors is crucial in mitigating the food insecurity and nutrition challenges resulting from low-N deficiency and Striga infestation. One hundred and fifty hybrids plus six hybrid checks were evaluated under low-N, Striga-infested and optimal conditions in Nigeria for two years. The study examined the combining ability of the PVA inbreds in contrasting environments, classified them into heterotic groups, examined the inter-relationships of grain yield and other agronomic traits of the inbreds in hybrid combinations and assessed the performance and stability of the PVA hybrids across environments. Additive gene action conditioned the inheritance of grain yield under Striga infestation and optimal conditions while non-additive gene action played the major role in the inheritance of grain yield under low-N. Hybrids TZEEIOR 217 × TZEEIOR 197 and TZEEIOR 245 × TZEEIOR 195 were the top-yielding under Striga and low-N conditions, respectively. Inbred lines were classified into three heterotic groups. Inbreds TZEEIOR 195 and TZEEIOR 221 were identified as testers while TZEEIOR 197 × TZEEIOR 82, TZEEIOR 132 × TZEEIOR 195 and TZEEIOR 205 × TZEEIOR 221 were identified as single-cross testers. Ear aspect had direct contribution to grain yield, justifying its inclusion in the multiple trait base index used for selection of Striga resistant and low-N tolerant maize genotypes in SSA. Hybrids TZEEIOR 195 × TZEEIOR 149, TZEEIOR 195 × TZEEIOR 150, TZEEIOR 245 × TZEEIOR 195, TZEEIOR 30 × TZEEIOR 150 and TZEEIOR 245 × TZEEIOR 30 were high-yielding and stable across test environments. These hybrids should be tested extensively in on-farm trials and commercialized to contribute to food security in SSA

Genotypic variability and stability of fibre yield components in kenaf (Hibiscus cannabinus L.)

Multi-environment yield trials (METs) are essential to reduce the challenges of the genotypes × environment interaction (G × E) in identification of promising genotypes. Therefore, this study estimated genetic variability, broad-sense heritability (h2b), genetic advances (GA) and repeatability as well as stability of core and bast fibres yield of 33 kenaf genotypes evaluated across six locations. The varieties were grown in an experiment laid out in randomized complete block design with three replications in 2016. Mature plants were harvested, retted and dried. Core fibre (CF) and bast fibre (BF) were weighed and data collected were subjected to analysis of variance (ANOVA) separately and across locations. Genetic variances, broad-sense heritability (h2b), genetic advance (GA) and repeatability were estimated for both traits. The G × E was assessed using joint regression analysis. Highly significant variation existed for both core and bast fibre yields among the genotypes in each of the locations. Phenotypic parameters were higher than those of the genotypes for both core fibre weight (CRW) and bast fibre weight (BSW). Broad-sense heritability was 82% for CRW and 66% for BSW while GA was 53.7% and 8.8% for CRW and BSW, respectively. Combined ANOVA showed significant differences for genotypes, environments, and G × E for CRW and BSW (p<0.001). Partitioning of G × E showed that only Gen × Env. (linear) was significant (P<0.05). Genotypic variance was 85% of the phenotypic variance for CRW, but only 32% for BSW. Hence, environmental effect was more on the CRW than BSW. Inheritance of the CRW and BSW is genetic because of their high heritability. Genetic advance and repeatability were higher for CRW than BSW. Based on high heritability with high GA for CRW, gene action governing inheritance of CRW is additive. Both additive and non-additive gene actions are responsible for BSW because its high heritability but low GA. Progress can therefore be made in selection for CRW than BSW in kenaf improvement programmes. Linear response of genotypes on environments accounted for the major part of total variation for the two types of fibre yield. Genotypes A-60-282-15, AC-313-293, AU-2452-5A and Cuba 108 are adapted to favourable environments while AU-2452-43, AU-24526, 2QQ 171 and AU-7192 are adapted to less favourable environments both in CF and BF yields.Keywords: Bast fibre, Core fibre, Genotype, Gene action, Regression analysis, Stabilit

Phenotypic status of elbow dysplasia in Boerboel dogs in Nigeria

Elbow dysplasia (ED) is a developmental, multifactorial, polygenic disease of rapidly growing, large breed of dogs. ED can be a significant health concern for affected dogs because treatments are largely ineffective, making selectively breeding of normal dogs the best option for disease control. Despite the relative availability of data on ED prevalence in other breeds, there is dearth of information on ED  prevalence in Boerboel dogs. This study evaluated ED prevalence and relative percentages of different ED grades in Boerboel dogs using radiographs of dogs presented for screening between April 2016 and September 2020. Extended latero-medial, flexed latero-medial and cranio-lateral-caudo-medial oblique radiographs from 64 dogs were reviewed. Radiographs were graded using international elbow working group (IEWG) criteria and grades 0-3 assigned to each joint. ED prevalence expressed as percentages were obtained by dividing the number of dogs with grades 1, 2 and 3 by total number of dogs. Age and sex difference in ED prevalence were compared using chi-square test. Differences were considered significant at P ≤ 0.05. Mean age of the dogs was 2.3 ± 0.6 years. Thirty-six (56.3%) dogs were graded as non-dysplastic, while 28(43.7%) were graded as dysplastic, with grade 3 (46.4%) accounting for majority of ED cases. There were no significant (p > 0.05) difference in sex and age prevalence of ED in the breed. In conclusion, ED prevalence in Boerboel dog is high with majority of dogs having severe grade. Widespread phenotypic screening and strict breeding restrictions are recommended| to control the disease in the breed. Keywords: Boerboel, Dog, Elbow dysplasia, Prevalence, Radiographic screenin