PROGRAM PEMERINTAH KABUPATEN KEPULAUAN TALAUD DALAM MEMAKSIMALKAN PEMANFAATAN POTENSI TANAMAN LOKAL (Suatu Studi Di Dinas Ketahanan Pangan dan Pertanian tentang Memaksimalkan Pemanfaatan Serat Pisang Abaka di Kecamatan Essang)

Sumber daya alam merupakan sumber daya yang sangat penting bagi keberlangsungan hidup manusia. Hilang atau berkurangnya ketersediaan sumber daya alam tersebut akan berdampak terhadap kelangsungan hidup manusia, pemanfaatan sumberdaya yang dimiliki oleh alam perlu dilakukan dalam rangka meningkatkan kesejahteraan masyarakat. Oleh karena itu, yang menjadi persoalan mendasar sehubungan dengan pengelolaan sumber daya alam adalah bagaimana mengelola sumber daya alam tersebut, agar menghasilkan manfaat yang sebesar-besarnya bagi manusia tanpa mengorbankan kelestarian sumber daya alam itu sendiri. Kabupaten Kepulauan Talaud Provinsi Sulawesi Utara berada diujung selatan Indonesia dan berbatasan langsung dengan Negara Filipina. Meskipun berada di daerah kepulauan yang identic dengan pulau karang dan lautan, namun Kabupaten Talaud menyimpan potensi sumberdaya alam yang luar biasa yang tidak dimiliki oleh kebanyakan daerah di Indonesia. Tujuan Penelitian ini adalah untuk mengetahui pelaksanaan program Pemerintah Daerah dalam memaksimalkan serat pisang Abaka di Kecamatan Essang Kabupaten Kepulauan Talaud, dengan menggunakan metode penelitian kualitatif diharapkan dapat menemukan jawaban terhadap masalah penelitian, hasil penelitian menunjukkan bahwa sarana dan prasarana merupakan salah satu aspek yang tidak dapat diabaikan dalam produksi serta pisang abaka, dalam rangka memenuhi aspek tersebut, pemerintah kabupaten mendapatkan bantuan dari Bank Indonesia berupa peralatan mesin yang diberikan kepada kelompok tani dan hasilnya cukup untuk meningkatkan produksi namun masih terkendala jumlah tempat produksi masih sedikit dan masih terpusat di satu tempat serta untuk mengangkut serat pisang abaka masih melalui teknik konvensional yakni melalui sungai karena infrastruktur jalan yang belum memadai.Kata Kunci: Program Pemerintah, Pemanfaatan, Tanaman Lokal

Mindfulness Factors and Anxiety Levels in Groups of Adults with Different Meditation Experience

The study includes the methodological model of mindfulness, definition of mindfulness in Latvian, model of other psychological notions related to mindfulness, proven links between the mindfulness and its subscales and anxiety scales, differences in mindfulness indicators among respondent groups with different meditation experience. The study involved 198 respondents in age of 22–56 years, M=36.91; SD=8.23, with different meditation experience. The study uses the Five Facet Mindfulness Questionnaire – FFMQ- adapted in Latvian by M. Majors (2012), as well as Spielberger’s State-Trait Anxiety Inventory Form (Spielberger, et al. 1983), adapted in Latvian by D. Škuškovnika (LU Rīga, 2004). The results show that there are statistically significant links between indicators of mindfulness and its subscales, as well as indicators of the anxiety scale (r between -0.27 and -0.63r, p less then 0.01). Results indicate statistically significant differences in the respondent groups of mindfulness and its subscales with different meditation practice. Results were between (F(2.198) is 6.51, p less then 0.05) and (F(2.198) is 19.83, p less then 0.01)

Acceptance of low-sugar yoghurt among Latvian teenagers

Over a thousand year history, yoghurt has become one of a widely consumed product in the world. Its reputation as a healthy food has been undermined recently by concerns over the high sugar content. The majority of consumers expects and prefers yoghurts to be sweet. However, governments across Europe are calling for significant cuts in the amount of added sugar used in yoghurt production. The aim of the study was to evaluate the acceptance of low-sugar yoghurt produced by different commercial β-galactosidases by teenagers. Standardised milk with fat content 2.0% (SC Tukuma piens) was pasteurized at 95 ± 1 °C 5 min, cooled down till 43 ± 1 °C and fermented with β-galactosidase and starter YC-X11 (Chr. Hansen, Denmark) and fermented till pH 4.50 ± 0.20. Different commercial β-galactosidases: Nola™ Fit 5500, Ha-Lactase 5200 (Chr. Hansen, Denmark), GODO-YNL2 (Danisco, Denmark) and BrennZyme (Brenntag PolskaSp, Poland) were used. Fermented samples were gently mixed and cooled down till 6 ± 1 °C and 5% (w/w) of sugar was added to each sample. Sensory evaluation of the yoghurt’s samples was performed by teenagers (14–18 years, n = 50) at Aizputes Secondary School (Latvia). Lactose and monosaccharides concentration prior to sugar addition was detected by HPLC (Shimadzu LC 20 Prominence, Japan). The lactose hydrolysis into glucose and galactose by the use of β-galactosidase helps to increase sweetness through an occurrence of natural sugars in milk. During sensory evaluation, teenagers admitted the yoghurt with reduced sugar as sweet, significantly sweeter (P < 0.05) was yoghurt sample with Nola™ Fit 5500. The results demonstrated that it is possible to reduce sugar in yoghurt production and to gain consumer acceptance through the occurrence of glucose and galactose, but it is problematic to offer lactose-free or reduced lactose products to consumers without lactose intolerance

The Development of Mindful-Based Dance Movement Therapy Intervention for Chronic Pain: A Pilot Study with Chronic Headache Patients

Funding Information: The study is part of a doctoral study that is financially supported by Riga Stradins University. Publisher Copyright: © Copyright © 2021 Majore-Dusele, Karkou and Millere.Chronic pain is of significant global concern. There is growing evidence that body–mind therapies and psychological approaches can contribute toward changing chronic pain perceptions. This is the first model described in the literature that combines a mindfulness-based approach with dance movement therapy and explores the potential psychological and pain-related changes for this client population. In this paper, the results from the pilot study are presented involving patients with chronic headache recruited in an outpatient rehabilitation setting. Methods: In this pilot study, 29 patients (n = 29) with chronic headache were randomized to either the Mindful-Based Dance Movement Therapy (MBDMT) group or the waiting list control group (treatment as usual, TAU). The MBDMT group was offered 10 sessions in a clinical outpatient rehabilitation setting for 5 weeks. Data were collected pre- and post-intervention and 16 weeks after the intervention was finished. The Hospital Anxiety and Depression Scale (HADS), Patient Health Questionnaire−9 (PHQ-9), Five Facet Mindfulness Questionnaire (FFMQ), and Numeric Rating Scale (NRS) were used as outcome measures. Results: The working model of MBDMT identifies nine therapeutic mechanisms (safe therapeutic environment, mindfulness skills, body awareness, relaxation/releasing, distancing and staying with discomfort, meaning making, self-regulation, acceptance and integration, creative process). Per-protocol analysis reveals statistically significant reduction of pain intensity and depression scores in favor of the MBDMT group, and these improvements were maintained in the follow-up assessment. Conclusions: The results suggest that MBDMT is a feasible and promising therapy approach for chronic pain patients. The pilot study offered sufficient information and preliminary results in the desirable direction to enable the researchers to move to a randomized controlled trial (RCT) stage in order to establish the efficacy of the intervention. Clinical Trial Registration: The study was registered in the www.researchregistry.com, registry (5483).publishersversionPeer reviewe

Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord

Background: A variety of cell types can be identified in the adherent fraction of bone marrow mononuclear cells including more primitive and embryonic-like stem cells, mesenchymal stem cells (MSC), lineage-committed progenitors as well as mature cells such as osteoblasts and fibroblasts. Different methods are described for the isolation of single bone marrow stem cell subpopulations - beginning from ordinary size sieving, long term cultivation under specific conditions to FACS-based approaches. Besides bone marrow-derived subpopulations, also other tissues including human umbilical cord (UC) have been recently suggested to provide a potential source for MSC. Although of clinical importance, these UC-derived MSC populations remain to be characterized. It was thus the aim of the present study to identify possible subpopulations in cultures of MSC-like cells obtained from UC. We used counterflow centrifugal elutriation (CCE) as a novel strategy to successfully address this question. Results: UC-derived primary cells were separated by CCE and revealed differentially-sized populations in the fractions. Thus, a subpopulation with an average diameter of about 11 μm and a small flat cell body was compared to a large sized subpopulation of about 19 μm average diameter. Flow cytometric analysis revealed the expression of certain MSC stem cell markers including CD44, CD73, CD90 and CD105, respectively, although these markers were expressed at higher levels in the small-sized population. Moreover, this small-sized subpopulation exhibited a higher proliferative capacity as compared to the total UC-derived primary cultures and the large-sized cells and demonstrated a reduced amount of aging cells. Conclusion: Using the CCE technique, we were the first to demonstrate a subpopulation of small-sized UC-derived primary cells carrying MSC-like characteristics according to the presence of various mesenchymal stem cell markers. This is also supported by the high proliferative capacity of these MSC-like cells as compared to whole primary culture or other UC-derived subpopulations. The accumulation of a self-renewing MSC-like subpopulation by CCE with low expression levels of the aging marker senescence-associated β-galactosidase provides a valuable tool in the regenerative medicine and an alternative to bone-marrow-derived MSC.DFG/KA 1784/5-

Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells

Following cultivation of distinct mesenchymal stem cell (MSC) populations derived from human umbilical cord under hypoxic conditions (between 1.5% to 5% oxygen (O2)) revealed a 2- to 3-fold reduced oxygen consumption rate as compared to the same cultures at normoxic oxygen levels (21% O2). A simultaneous measurement of dissolved oxygen within the culture media from 4 different MSC donors ranged from 15 μmol/L at 1.5% O2 to 196 μmol/L at normoxic 21% O2. The proliferative capacity of the different hypoxic MSC populations was elevated as compared to the normoxic culture. This effect was paralleled by a significantly reduced cell damage or cell death under hypoxic conditions as evaluated by the cellular release of LDH whereby the measurement of caspase3/7 activity revealed little if any differences in apoptotic cell death between the various cultures. The MSC culture under hypoxic conditions was associated with the induction of hypoxia-inducing factor-alpha (HIF-1α) and an elevated expression of energy metabolism-associated genes including GLUT-1, LDH and PDK1. Concomitantly, a significantly enhanced glucose consumption and a corresponding lactate production could be observed in the hypoxic MSC cultures suggesting an altered metabolism of these human stem cells within the hypoxic environment

Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord

<p>Abstract</p> <p>Background</p> <p>A variety of cell types can be identified in the adherent fraction of bone marrow mononuclear cells including more primitive and embryonic-like stem cells, mesenchymal stem cells (MSC), lineage-committed progenitors as well as mature cells such as osteoblasts and fibroblasts. Different methods are described for the isolation of single bone marrow stem cell subpopulations – beginning from ordinary size sieving, long term cultivation under specific conditions to FACS-based approaches. Besides bone marrow-derived subpopulations, also other tissues including human umbilical cord (UC) have been recently suggested to provide a potential source for MSC. Although of clinical importance, these UC-derived MSC populations remain to be characterized. It was thus the aim of the present study to identify possible subpopulations in cultures of MSC-like cells obtained from UC. We used counterflow centrifugal elutriation (CCE) as a novel strategy to successfully address this question.</p> <p>Results</p> <p>UC-derived primary cells were separated by CCE and revealed differentially-sized populations in the fractions. Thus, a subpopulation with an average diameter of about 11 μm and a small flat cell body was compared to a large sized subpopulation of about 19 μm average diameter. Flow cytometric analysis revealed the expression of certain MSC stem cell markers including CD44, CD73, CD90 and CD105, respectively, although these markers were expressed at higher levels in the small-sized population. Moreover, this small-sized subpopulation exhibited a higher proliferative capacity as compared to the total UC-derived primary cultures and the large-sized cells and demonstrated a reduced amount of aging cells.</p> <p>Conclusion</p> <p>Using the CCE technique, we were the first to demonstrate a subpopulation of small-sized UC-derived primary cells carrying MSC-like characteristics according to the presence of various mesenchymal stem cell markers. This is also supported by the high proliferative capacity of these MSC-like cells as compared to whole primary culture or other UC-derived subpopulations. The accumulation of a self-renewing MSC-like subpopulation by CCE with low expression levels of the aging marker senescence-associated β-galactosidase provides a valuable tool in the regenerative medicine and an alternative to bone-marrow-derived MSC.</p

Koordinasi Pemerintah Daerah dalam Pembangunan Jalan di Kecamatan Salibabu Kabupaten Kepulauan Talaud Coordination Of Local Government In Road Construction In Kecamatan Salibabu Kabupaten Kepulauan Talaud

The purpose of this study is to know how the coordination of local goverment in road constructionin the District of Salibabu Talaud Islands Regency.This research uses the qualitative method. Data collection by interview technique: while dataanalysis using qualitative analysis techniques interactive model of Miles and Hubernann.Based on research results drawn conclusions: (1) Coordination of district road constructionplanning in sub-districts of Salibabu run orderly and smoothly in accordance with the mechanism that hasbeen set in UU.No.25 Tahun 2004 about The National Development Planning System. Related governmentinstallations (Dinas PUTR, Bappeda, and Camat) coordinate well so that the district road developmentprogram can be set according to the priority scale. Coordination mechanism of district road developmentplaning in Salibabu sub-distric implemented accordingto the bottom. (2) Coordination of local government(Dinas PUTR and Camat, and also with Kepala Desa) in the implementation of road construction programsor projects in the District of Salibabu Talaud Islands Regency well done and running smoothly

Molecular analysis of sarcomeric and non-sarcomeric genes in patients with hypertrophic cardiomyopathy.

Background: Hypertrophic cardiomyopathy (HCM) is a common genetic heart disorder characterized by unexplained left ventricle hypertrophy associated with non-dilated ventricular chambers. Several genes encoding heart sarcomeric proteins have been associated to HCM, but a small proportion of HCM patients harbor alterations in other non-sarcomeric loci. The variable expression of HCM seems influenced by genetic modifier factors and new sequencing technologies are redefining the understanding of genotype–phenotype relationships, even if the interpretations of the numerous identified variants pose several challenges. Methods and results: We investigated 62 sarcomeric and non-sarcomeric genes in 41 HCM cases and in 3 HCM-related disorders patients. We employed an integrated approach that combines multiple tools for the prediction, annotation and visualization of functional variants. Genotype–phenotype correlations were carried out for inspecting the involvement of each gene in age onset and clinical variability of HCM. The 80% of the non-syndromic patients showed at least one rare non-synonymous variant (nsSNV) and among them, 58% carried alterations in sarcomeric loci, 14% in desmosomal and 7% in other non-sarcomeric ones without any sarcomere change. Statistical analyses revealed an inverse correlation between the number of nsSNVs and age at onset, and a relationship between the clinical variability and number and type of variants. Conclusions: Our results extend the mutational spectrum of HCM and contribute in defining the molecular pathogenesis and inheritance pattern(s) of this condition. Besides, we delineate a specific procedure for the identification of the most likely pathogenetic variants for a next generation sequencing approach embodied in a clinical context

Los embera katio del Alto Sinú- Córdoba

De Santa Marta a Montería; de Montería a Tierralta; de Tierralta a Puerto Frasquillo; de Puerto Frasquillo, en Johnson, hasta Pawarandó, y de Pawarandó a Begui- dó. Más de ocho horas en flota y lancha por el rio Zenú, el rio Esmeraldas y el río Verde. Era nuestra primera vez por estos lados del Caribe colombiano. No puedo negar mi temor en estas tierras, cuando las noticias escritas y visuales son muchas: el paramilitarismo en Tierralta (Córdoba) fue una peste que aún no ha terminado. Buena parte de este país se ha deconstruido (o más bien destruido) a partir del rumor, del chisme. Hay algo interesante que me pasa y creo que les pasa a los jóvenes investigadores con los que siempre salgo de campo y es que cuando estamos en los lugares que históricamente han sido señalados de violentos, nos encontramos con todo lo contrario (creo que es suerte): han sido territorios seguros y amables. &nbsp