Macroevolutionary patterns in cranial and lower jaw shape of ceratopsian dinosaurs (dinosauria, ornithischia). phylogeny, morphological integration, and evolutionary rates

Organisms: Ceratopsians were herbivorous, beaked dinosaurs, ranging from 1 m to 9 m in body length, usually four-footed, and with a bony frill that extended backwards from the cranium over the nape of the neck. Known from Asia, Europe, and North America, they appeared in the Late Jurassic and persisted until the end of the Late Cretaceous. Questions: Which evolutionary processes drive the phenotypic evolution of skulls and lower jaws within Ceratopsia? What is the degree of morphological integration between the skull and lower jaw, and between the snout and frill among clades? Finally, are there any morphological evolution rate shifts across the ceratopsian phylogeny? Data: Photographs from 121 ceratopsian skulls and 122 lower jaws in lateral view, both from original photos and published pictures. Fifty-five ceratopsian species are represented in the sample. Methods: We investigated cranial and lower jaw shape changes across ceratopsians applying two-dimensional geometric morphometrics. We also investigated the morphological variation of the snout and the frill. Using phylogenetic generalized least squares regression, we estimated the degree of phylogenetic signal in size and shape data, as well as in the shape-size relationship. We performed phenotypic evolutionary rate analysis on shape data to describe phenotypic shifts across the phylogeny. Using a rarefied version of Escoufier's RV coefficient, we tested morphological integration between skulls and lower jaws, and between snouts and frills. Finally, we explored the potential link between cranial and frill shape evolution in ceratopsians and the radiation of angiosperms using a linear regression model. Results: Skull, snout, and frill shapes differ among clades (with the exception of leptoceratopsids and protoceratopsids). Lower jaws show distinct morphologies among groups. Size and shape changes are phylogenetically structured. The frill drives the morphological variation of the skull, co-varying much more with the lower jaw than with the snout. The frill appears to evolve to co-vary better with the lower jaw in the more morphologically derived clades than in basal ones. A significant linear relationship does exist between cranial shape and angiosperm occurrences, suggesting the hypothesis that the frill evolved in response to changes in dietary compositions associated with the turnover between gymnosperms and angiosperms during the Cretaceous. Significant negative shifts in evolutionary rates characterize skull, snout, frill, and lower jaw shapes, corresponding to nodes where psittacosaurids diverge from other taxa. In contrast, a significant positive shift in skull and snout shape rate of evolution characterizes the clade Ceratopsoidea. Conclusion: The frill is the main driving force in the overall cranial shape within Ceratopsia and evolved secondarily to better co-vary with the lower jaw to produce a more efficient masticatory apparatus. The changes in frill shape are correlated with the angiosperm diversification that occurred in the Cretaceous and thus correlated with changes in diet. Ceratopsians exhibit a slowdown in the phenotypic evolutionary rate in the Early Cretaceous and an acceleration of the phenotypic rate in the Late Cretaceous

Pequeños vertebrados del relleno kárstico del Pleistoceno Superior de Avetrana (Apulia, Sur de Italia)

The fossiliferous deposit (karst cavity) in La Grave, a locality near the small town of Avetrana (Taranto, south­ern Italy), has yielded numerous fossils of vertebrates. The remains of large mammals have been the subject of several studies. This paper examines the remains of small vertebrates and identifies four taxa of amphibians (Bufo bufo, Bufotes gr. B. viridis, Hyla gr. H. Arborea and Rana (s.l.) sp.), four taxa of reptiles (Testudo hermanni, Podarcis sp., Zamenis gr. Z. longissimus, Natrix natrix), and nine taxa of small mammals (Erinaceus europaeus, Crocidura suaveolens, Arvicola italicus, Microtus (Terricola) savii, Microtus (Microtus) arvalis, Apodemus gr. A. sylvaticus - A. flavicollis, Hystrix (Acanthion) vinogradovi, Oryctolagus cuniculus and Lepus corsicanus). From a biochronological point of view, the data on small and large vertebrates indicate an age between the beginning of the Late Pleistocene (MIS 5e) and the central part of MIS 3. The most recent fossiliferous layer (bed 8) is likely to have been deposited during a cooler period when compared to the previous layers.The data from small fossil vertebrates combined with those emerging from the large mammals and birds evidence the presence, near the karstic cavity, of open spaces (prairies) with pools of water, bordered by wooded areas and, not far, the presence of a rocky coastline.El depósito (cavidad kárstica) de La Grave, localidad cercana a la pequeña ciudad de Avetrana (Tarento, Italia meridional), ha dado lugar a numerosos fósiles de vertebrados. Los restos de grandes mamíferos han sido objeto de varios estudios. En este trabajo se examinan los restos de pequeños vertebrados y se identifican cuatro taxones de anfibios (Bufo bufo, Bufotes gr. B. viridis, Hyla gr. H. Arborea and Rana (s.l.) sp.), cuatro de reptiles (Testudo hermanni, Podarcis sp., Zamenis gr. Z. longissimus, Natrix natrix), y nueve de pequeños mamíferos (Erinaceus europaeus, Crocidura suaveolens, Arvicola italicus, Microtus (Terricola) savii, Microtus (Microtus) arva­lis, Apodemus gr. A. sylvaticus - A. flavicollis, Hystrix (Acanthion) vinogradovi, Oryctolagus cuniculus and Lepus corsicanus). Desde un punto de vista biocronológico, los datos sobre los vertebrados pequeños y grandes indican una edad entre el comienzo del Pleistoceno tardío (MIS 5e) y la parte central del MIS 3. Es probable que el estrato fosilífero más reciente (nivel 8) se haya depositado durante un período más frío en comparación con las capas anteriores. Los datos de pequeños vertebrados fósiles combinados con los que proceden de los grandes mamífe­ros y aves evidencian la presencia, cerca de la cavidad kárstica, de espacios abiertos (praderas) con charcos de agua, bordeados por zonas boscosas y, no muy lejos, la presencia de una costa rocosa

Lentiviral-mediated administration of IL-25 in the CNS induces alternative activation of microglia

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Small vertebrates from the Late Pleistocene of Avetrana (Apulia, southern Italy) karst filling

The fossiliferous deposit (karst cavity) in La Grave, a locality near the small town of Avetrana (Taranto, southern Italy), has yielded numerous fossils of vertebrates. The remains of large mammals have been the subject of several studies. This paper examines the remains of small vertebrates and identifies four taxa of amphibians (Bufo bufo, Bufotes gr. B. viridis, Hyla gr. H. Arborea and Rana (s.l.) sp.), four taxa of reptiles (Testudo hermanni, Podarcis sp., Zamenis gr. Z. longissimus, Natrix natrix), and nine taxa of small mammals (Erinaceus europaeus, Crocidura suaveolens, Arvicola italicus, Microtus (Terricola) savii, Microtus (Microtus) arvalis, Apodemus gr. A. sylvaticus - A. flavicollis, Hystrix (Acanthion) vinogradovi, Oryctolagus cuniculus and Lepus corsicanus). From a biochronological point of view, the data on small and large vertebrates indicate an age between the beginning of the Late Pleistocene (MIS 5e) and the central part of MIS 3. The most recent fossiliferous layer (bed 8) is likely to have been deposited during a cooler period when compared to the previous layers.The data from small fossil vertebrates combined with those emerging from the large mammals and birds evidence the presence, near the karstic cavity, of open spaces (prairies) with pools of water, bordered by wooded areas and, not far, the presence of a rocky coastline

Selenoprotein gene nomenclature

The human genome contains 25 genes coding for selenocysteine-containing proteins (selenoproteins). These proteins are involved in a variety of functions, most notably redox homeostasis. Selenoprotein enzymes with known functions are designated according to these functions: TXNRD1, TXNRD2, and TXNRD3 (thioredoxin reductases), GPX1, GPX2, GPX3, GPX4 and GPX6 (glutathione peroxidases), DIO1, DIO2, and DIO3 (iodothyronine deiodinases), MSRB1 (methionine-R-sulfoxide reductase 1) and SEPHS2 (selenophosphate synthetase 2). Selenoproteins without known functions have traditionally been denoted by SEL or SEP symbols. However, these symbols are sometimes ambiguous and conflict with the approved nomenclature for several other genes. Therefore, there is a need to implement a rational and coherent nomenclature system for selenoprotein-encoding genes. Our solution is to use the root symbol SELENO followed by a letter. This nomenclature applies to SELENOF (selenoprotein F, the 15 kDa selenoprotein, SEP15), SELENOH (selenoprotein H, SELH, C11orf31), SELENOI (selenoprotein I, SELI, EPT1), SELENOK (selenoprotein K, SELK), SELENOM (selenoprotein M, SELM), SELENON (selenoprotein N, SEPN1, SELN), SELENOO (selenoprotein O, SELO), SELENOP (selenoprotein P, SeP, SEPP1, SELP), SELENOS (selenoprotein S, SELS, SEPS1, VIMP), SELENOT (selenoprotein T, SELT), SELENOV (selenoprotein V, SELV) and SELENOW (selenoprotein W, SELW, SEPW1). This system, approved by the HUGO Gene Nomenclature Committee, also resolves conflicting, missing and ambiguous designations for selenoprotein genes and is applicable to selenoproteins across vertebrates

MRX87 family with Aristaless X dup24bp mutation and implication for polyAlanine expansions

<p>Abstract</p> <p>Background</p> <p>Cognitive impairments are heterogeneous conditions, and it is estimated that 10% may be caused by a defect of mental function genes on the X chromosome. One of those genes is <it>Aristaless related homeobox </it>(<it>ARX</it>) encoding a polyA-rich homeobox transcription factor essential for cerebral patterning and its mutations cause different neurologic disorders. We reported on the clinical and genetic analysis of an Italian family with X-linked mental retardation (XLMR) and intra-familial heterogeneity, and provided insight into its molecular defect.</p> <p>Methods</p> <p>We carried out on linkage-candidate gene studies in a new MRX family (MRX87). All coding regions and exon-intron boundaries of ARX gene were analysed by direct sequencing.</p> <p>Results</p> <p>MRX87 patients had moderate to profound cognition impairment and a combination of minor congenital anomalies. The disease locus, MRX87, was mapped between DXS7104 and DXS1214, placing it in Xp22-p21 interval, a hot spot region for mental handicap. An in frame duplication of 24 bp (ARXdup24) in the second polyAlanine tract (polyA_II) in ARX was identified.</p> <p>Conclusion</p> <p>Our study underlines the role of ARXdup24 as a critical mutational site causing mental retardation linked to Xp22. Phenotypic heterogeneity of MRX87 patients represents a new observation relevant to the functional consequences of polyAlanine expansions enriching the puzzling complexity of ARXdup24-linked diseases.</p

Breast cancer "tailored follow-up" in Italian oncology units: a web-based survey

urpose: Breast cancer follow-up procedures after primary treatment are still a controversial issue. Aim of this study was to investigate, through a web-based survey, surveillance methodologies selected by Italian oncologists in everyday clinical practice. Methods: Referents of Italian medical oncology units were invited to participate to the study via e-mail through the SurveyMonkey website. Participants were asked how, in their institution, exams of disease staging and follow-up are planned in asymptomatic women and if surveillance continues beyond the 5th year. Results: Between February and May 2013, 125 out of 233 (53.6%) invited referents of Italian medical oncology units agreed to participate in the survey. Ninety-seven (77.6%) referents state that modalities of breast cancer follow-up are planned according to the risk of disease progression at diagnosis and only 12 (9.6%) oncology units apply the minimal follow-up procedures according to international guidelines. Minimal follow-up is never applied in high risk asymptomatic women. Ninety-eight (78.4%) oncology units continue follow-up in all patients beyond 5 years. Conclusions: Our survey shows that 90.4% of participating Italian oncology units declare they do not apply the minimal breast cancer follow-up procedures after primary treatment in asymptomatic women, as suggested by national and international guidelines. Interestingly, about 80.0% of interviewed referents performs the so called "tailored follow-up", high intensity for high risk, low intensity for low risk patients. There is an urgent need of randomized clinical trials able to determine the effectiveness of risk-based follow-up modalities, their ideal frequency and persistence in time