Uno spettacolo a misura dei “particolari”: “Con chi vengo vengo” a palazzo Girifalco (Napoli 1665)

En el año 1665 el palacio del duque de Girifalco acoge a unos nobles napolitanos seleccionados para exhibir su magnificencia y su lealtad a la corona de España con un espectáculo muy rico sobre la obra de Calderón “Con quien vengo vengo”. Esta ocasión atestigua el mito de Calderón en Nápoles a lo largo del siglo XVII y las técnicas representativas en las casas aristocráticas. El texto fue puesto en escena utilizando las estrategias típicas del teatro napoletano, con prólogos y «licenze» musicales, bailes entre los actos e idiomas múltiples, y entregado a una compañía de valientes diletantes y profesionales talentosos. Entre «mensajes» performativos y otros se celebra una liturgia de propaganda, en la que se encuentran todos los elementos que amplifican y subrayan el homenaje del duque y de sus huéspedes, que se reconocen en él, al poder español. El esplendor y el mito de la «cattolicissima» ciudad se constituye a través de ocasiones parecidas, que unen los lenguajes más diferentes y la experimentación más atrevida. En este tiempo Nápoles anda perfeccionando y definiendo un papel artístico que en unas décadas se convertirá en una primacía absoluta en toda Europa.In 1665 the palace of the Duke of Girifalco hosts a selected group of aristocratic people for a rich performance inspired to Calderón’s “Con quien vengo vengo”, in order to show the magnificence of the house as well as its loyalty to the Spanish king. This event testifies both to the myth of Calderón in Naples in the 17th century and to the performing strategies in private houses. The text was staged using typical resources of Neapolitan theatres, such as prologues, musical insertions, dances between the acts and multiple language scenes, and was entrusted to a troupe formed by talented amateurs and skilled practitioners. Technical solutions and other «messages» constitute a propagandistic «liturgy» aimed at emphasizing the tribute of the Duke (and of his entourage) to the Spanish power. The glamour and the myth of the «catholic» nation is based also upon such events, where different «languages» and bold experiments are combined. At that time Naples is gaining (and bringing to perfection) an artistic role, which will soon turn into an absolute primacy in Europe

Targeting the MET oncogene by concomitant inhibition of receptor and ligand via an antibody-“decoy” strategy

MET, a master gene sustaining "invasive growth," is a relevant target for cancer precision therapy. In the vast majority of tumors, wild-type MET behaves as a "stress-response" gene and relies on the ligand (HGF) to sustain cell "scattering," invasive growth and apoptosis protection (oncogene "expedience"). In this context, concomitant targeting of MET and HGF could be crucial to reach effective inhibition. To test this hypothesis, we combined an anti-MET antibody (MvDN30) inducing "shedding" (i.e., removal of MET from the cell surface), with a "decoy" (i.e., the soluble extracellular domain of the MET receptor) endowed with HGF-sequestering ability. To avoid antibody/decoy interaction-and subsequent neutralization-we identified a single aminoacid in the extracellular domain of MET-lysine 842-that is critical for MvDN30 binding and engineered the corresponding recombinant decoyMET (K842E). DecoyMET(K842E) retains the ability to bind HGF with high affinity and inhibits HGF-induced MET phosphorylation. In HGF-dependent cellular models, MvDN30 antibody and decoyMET(K842E) used in combination cooperate in restraining invasive growth, and synergize in blocking cancer cell "scattering." The antibody and the decoy unbridle apoptosis of colon cancer stem cells grown in vitro as spheroids. In a preclinical model, built by orthotopic transplantation of a human pancreatic carcinoma in SCID mice engineered to express human HGF, concomitant treatment with antibody and decoy significantly reduces metastatic spread. The data reported indicate that vertical targeting of the MET/HGF axis results in powerful inhibition of ligand-dependent MET activation, providing proof of concept in favor of combined target therapy of MET "expedience.

Polymorphism of Genetic variability of gene in sheep

MUC1 gene encodes the polypeptide of a mucin present on the apical surface of the secreting mammary cells during lactation and on the surface of the milk fat globules (Mather, 2000). MUC1 contains a minisatellite region: the sequence, coding for the extracellular protein domain, consists mostly of a motif of 60 bp that in human, cattle and goat is tandemly repeated a variable number of times (VNTR polymorphism), giving rise to molecular variants of different size (Patton et al., 1995)

Structural assessment of the EU-DEMO WCLL Central Outboard Blanket segment under normal and off-normal operating conditions

Within the framework of the EUROfusion design activities concerning the EU-DEMO Breeding Blanket (BB) system, a research campaign has been carried out at the University of Palermo with the aim of investigating the structural behaviour of the DEMO Water-Cooled Lithium Lead (WCLL) Central Outboard Blanket (COB) segment. The assessment has been performed considering three different loading scenarios: the Normal Operation (NO), the Over-Pressurization (OP) and the Upward Vertical Displacement Event (VDE-up). In particular, NO scenario represents the loading case referring to the nominal operating conditions, whereas the OP scenario refers to the loading conditions due to an in-box LOCA accident, listed as one of the BB design basis accidental events. Lastly, the VDE-up scenario is an off-normal event reproducing the plasma disruption caused by an uncontrolled vertical motion of the plasma volume. This event brings the plasma in contact with the upper part of the plasma chamber, generating a sudden energy discharge accompanied by Electro Magnetic (EM) forces acting on the structure. The study has been carried out following a theoretical-numerical approach based on the Finite Element Method (FEM) and adopting the quoted ABAQUS v. 6.14 commercial FEM code. In particular, a detailed 3D FEM model of the whole COB segment, including the back-supporting structure and its attachment system to the vacuum vessel, has been set up. Several simulations have been run to assess the thermo-mechanical performances of the segment under the afore-mentioned loading scenarios, also taking into account the impact of the tungsten (W)-armour on the overall structural response. EM loads have been considered in all the assessed scenarios. In the first two, only magnetization forces have been taken into account, while in the VDE-up scenario Lorentz's forces have been also taken into account. The structural response has been evaluated according to the RCC-MRx structural design rules. The obtained results are herewith presented and critically discussed

High Grade Glioma Treatment in Elderly People: Is It Different Than in Younger Patients? Analysis of Surgical Management Guided by an Intraoperative Multimodal Approach and Its Impact on Clinical Outcome

Objective: Age is considered a negative prognostic factor for High Grade Gliomas (HGGs) and many neurosurgeons remain skeptical about the benefits of aggressive treatment. New surgical and technological improvements may allow extended safe resection, with lower level of post-operative complications. This opportunity opens the unsolved question about the most appropriate HGG treatment in elderly patients. The aim of this study is to analyze if HGG maximal safe resection guided by an intraoperative multimodal imaging protocol coupled with neuromonitoring is associated with differences in outcome in elderly patients versus younger ones. Methods: We reviewed 100 patients, 53 (53%) males and 47 (47%) females, with median (IQR) age of 64 (57; 72) years. Eight patients were diagnosed with Anaplastic Astrocytoma (AA), 92 with Glioblastoma (GBM). Surgery was aimed to achieve safe maximal resection. An intraoperative multimodal imaging protocol, including neuronavigation, neurophysiological monitoring, 5-ALA fluorescence, 11C MET-PET, navigated i-US system and i-CT, was used, and its impact on EOTR and clinical outcome in elderly patients was analyzed. We divided patients in two groups according to their age: 65 years, and surgical and clinical results (EOTR, post-operative KPS, OS and PFS) were compared. Yet, to better understand age-related differences, the same patient cohort was also divided into 70 years and all the above data reanalyzed. Results: In the first cohort division, we did not found KPS difference over time and survival analysis did not show significant difference between the two groups (p = 0.36 for OS and p = 0.49 for PFS). Same results were obtained increasing the age cut-off for age up to 70 years (p = 0.52 for OS and p = 0.92 for PFS). Conclusions: Our data demonstrate that there is not statistically significant difference in post-operative EOTR, KPS, OS, and PFS between younger and elderly patients treated with extensive tumor resection aided by a intraoperative multimodal protocol

Preoperative Localization in Colonic Surgery (PLoCoS Study): a multicentric experience on behalf of the Italian Society of Colorectal Surgery (SICCR)

The aim of this prospective multicentric study was to compare the accurate colonic lesion localization ratio between CT and colonoscopy in comparison with surgery. All consecutive patients from 1st January to 31st December 2019 with a histologically confirmed diagnosis of dysplastic adenoma or adenocarcinoma with planned elective, curative colonic resection who underwent both colonoscopy and CT scans were included. Each patient underwent conventional colonoscopy and CT to stage the tumour, and the localization results of each procedure were registered. CT and colonoscopic localization were compared with surgical localization, adopted as the reference. Our analysis included 745 patients from 23 centres. After comparing the accuracy of colonoscopy and CT (for visible lesions) in localizing colonic lesions, no significant differences were found between the two preoperative tools (510/661 vs 499/661 correctly localized lesions, p = 0.518). Furthermore, after analysing only the patients who underwent complete colonoscopy and had a visible lesion on CT, no significant difference was observed between conventional colonoscopy and CT (331/427 vs 340/427, p = 0.505). Considering the intraoperative localization results as a reference, a comparison between colonoscopy and CT showed that colonoscopy significantly failed to correctly locate the lesions localized in the descending colon (17/32 vs 26/32, p = 0.031). We did not identify an advantage in using CT to localize colonic tumours. In this setting, colonoscopy should be considered the reference to properly localize lesions; however, to better identify lesions in the descending colon, CT could be considered a valuable tool to improve the accuracy of lesion localizatio