First report of Longidorus mindanaoensis Coomans, De Ley, Jimenez and De Ley, 2012 (Nematoda: Longidoridae) from a mangrove forest in Vietnam

Longidorus mindanaoensis was recovered from a mangrove forest in Vietnam. The recovered population is in general morphological agreement with the type population, and the characters of pharyngeal bulb, i.e. the same unique pattern of pharyngeal glands nuclei as well as the lip region morphology, amphidial fovea shape and size and position of vulva corroborated its identity. Molecular studies of the recovered population using D2-D3 expansion segments of large subunit ribosomal DNA (LSU rDNA D2-D3) revealed the D2-D3 sequence of the recovered population is 99.6% similar to the sequence of the type population. A new morphometric range for body size was recorded for the species based upon present Vietnamese population, and the present study emphasized that the diversity of Longidorus spp. in Vietnam could be higher than previously assumed

Morphological diversity of Meloidogyne spp. from carrot (Daucus carota subsp. Sativus) in Vietnam

Carrot (Daucus carota subsp. Sativus) is known as one of the most widely cultivated and widely consumed vegetables in the world due to its nutritional and economic values. During a survey of nematodes parasites carrots from Vietnam, six populations of three species of root-knot nematodes, namely M. incognita, M. arenaria, and M. graminicola were found. The species specific primers were confirmed before morphological studies. By combining the morphology and morphometry of the females, males, and juveniles, this study provided useful references for classification of Meloidogyne on carrots in the future. Quantitative morphological studies reveal profound changes corresponding with the generation of morphological disparity at high taxonomic diversity. Especially, this study provided the first morphological and morphometric information of M. graminicola, that is known as aquatic root-knot nematodes, on carrots

A STUDY OF SUSTAINABLE COLORATION OF LYOCELL FABRICS USING EXTRACTS OF TROPICAL ONION SKINS

Lyocell is considered as a new fiber that represents a milestone in the development of environmentally sustainable textiles.  Lyocell is spun from wood pulp cellulose via a green chemical process with NMMO (N-methylmorpholine-N-oxide) solvent. Following the concept of lower the environmental impact of fashion clothing, this study aims to determine the suitable natural dyes recipes with the color extracting from tropical onion skins. Colorants were extracted by dissolving crushed dried onion skins with boiled in water at 100ºC for 20-25 minutes. The ratio of extracting and dyeing are 1:25 and 1:30 respectively. The optimal dyeing condition was found out at 80ºC, 45 minutes with 75% v/v. In addition, a variety of the most commonly used mordants including Potassium aluminum sulfate, Copper (II) sulphate and Iron (II) sulphate were used for mordanting in order to compare the differently mordanted and unmordanted dyed fabrics via color strength (K/S) and CIE L*a*b* color values. It was found that mordant type had an effect on color strength and the color coordinates of fabric dyed with onion skin, which can supply variety of color choices for the same colorants

ANTIBACTERIAL FINISHING ON COTTON 100% AND CVC FABRICS WITH TANNIN FROM PIPER BETLE EXTRACT

Following the recent trend of using natural ingredients from bio-macromolecules, biomaterials, plant extract in textile chain, this research aims to develop an antibacterial textiles finishing with Tannin extracted from piper betle plant. The extracting processes were carried out with different solvent: distilled water, Ethanol 30%, Ethanol 50%, Ethanol 70% in 60 minutes. Two important types of fabric, including Cotton and CVC (Cotton/Polyester) were padded with piper betle extracts, then dried at 60oC in 5 minutes. The presence of tannin on fabric after treatment was determined by FeCl3 test and FT-IR spectrum. The antibacterial effect of finished fabrics was proved according to ASTM 2149-01 standard. The test was performed with Escherichia Coli ATCC 25922 and Staphylococus aureus AATCC 6538. The final results exhibited good antibacterial activity of 83.02%, 65,33% against the bacteria Ecoli and 93.88 %, 85.14% against the bacteria S.Aureus on cotton and then CVC fabrics

Description of Geocenamus vietnamensis sp. n. (Nematoda : Merliniidae) from Vietnam

A new species of the genus Geocenamus was isolated from soil and root samples from the rhizosphere of Casuarina equisetifolia (Casuarinaceae) in Quang Nam province, Vietnam. This species is characterized by a round-to-hexagonal labial disc, the presence of a labial region, which is continuous or slightly offset from the body with six sectors, lateral sectors of first labial annulus being smaller than the submedian sectors, the presence of six to seven labial annules; the absence of deirids; stylet length 24 to 28 mu m long, body length 776 to 979 mu m long; lateral field with six to eight lateral lines, without areolation at mid-body and with areolation in outer bands at the tail region and a pointed tail terminus. Geocenamus vietnamensis n. sp. most closely resembles G. boghiae in having a non-sclerotized head framework and lacking a bursa in the males. It can be clearly distinguished from all other species of the genus Geocenamus by these characteristics. The combination of morphology, morphometric features, and phylogenetic trees, based on D2-D3 of 28S and ITS rDNA sequences, showed that this new species can be clearly separated from all other sequenced species. This record is the first for Geocenamus in Vietnam

First report of Mesocriconema sphaerocephalum (Taylor, 1936) Loof, 1989 associated with carrot (Daucus carota subsp. Stativus) in Vietnam

Our study recorded the presence of Mesocriconema sphaeroce phalum on carrot in Hanoi city and Hai Duong province in Vietnam. This species was identified by morphometric, morphological characterizations, and molecular characterization of D2D3 of 28S rDNA sequence. To our knowledge, this is the first report of M. sphaerocephalum on carrot in Vietnam

Morphological and molecular characteristics of Pratylenchus haiduongensis sp. n., a new species of root-lesion nematodes associated with carrot in Vietnam

Pratylenchus haiduongensis sp. n. is described as associated with carrot (Daucus carota subsp. sativus (Hoffm.) Schubl. & G. Martens) in Hai Duong Province, Vietnam. P. haiduongensis sp. n. is characterized by the lip region with three annuli and slightly separated from the body. Stylet knobs are rounded (never indented anteriorly). The lateral field includes four incisures, bearing areolation at the pharynx region and tail region and occasionally appears in the vulval region. Sometimes the appearances of oblique broken striaes divide the lateral field into five or six incisures. The ovary is distinct with one row of oocytes. Spermatheca is oval in shape with round central cavity, without sperm or reduced in some specimens. The postvuval uterine sac is long surpassing the vulva body diameter by 2 to 2.5 times (PUS = 31 to 65 mm). High vulva position with V = 66 to 75%. The tail shape can be subhemispherical with a smooth, slightly indented, broadly smooth, or cleft terminus observed in some specimens. The matrix code of P. haiduongensis sp. n. is: A2, B1, C4, D(1,3), E1, F(5,6), G(1,2), H(1,4); I(1,2,3,4), J1, K(1,2) according to Castillo and Vovlas (2007). The LSU-D2D3 segment and the ITS-rDNA region of this species were amplified and sequenced. The morphological characters and molecular phylogenetic analyses confirmed that this is a new species of the genus Pratylenchus in Vietnam

First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda : Longidoridae) in Vietnam

For the first time, a survey of plant-parasitic nematodes in the Central Highlands of Vietnam discovered a population of Xiphinema hunaniense Wang & Wu, 1992. The Vietnamese population of X. hunaniense is characterized by having an offset lip region, lack of anterior genital branch, vagina directed backward, and a digitate tail. Morphological features and morphometrics of this population are in agreement with the type population of X. hunaniense except for some variations. In addition, molecular characterization of this population and phylogenetic tree of 28S rDNA sequences of the genus are also provided

First report of Hemicriconemoides litchi associated with Piper sarmentosum and revision of the genus Hemicriconemoides in Vietnam

The taxonomic status of Hemicriconemoides litchi, H. mangiferae, and H. strictathecatus has been in debates for many years. In this study, a population of H. litchi collected from the rhizosphere of Piper sarmentosum is characterized using a combination of morphology and molecular data. Our results are in agreement with other authors to maintain the validity of H. litchi and provide sequences of H. litchi with correct names on GenBank. A revision on the genus Hemicriconemoides in Vietnam is also discussed