34 research outputs found
Microplastics in Ghanaian coastal lagoon sediments: Their occurrence and spatial distribution
This is the author accepted manuscript.Each year millions of tonnes of plastic are produced worldwide and around 8 million tons are deposited into our marine environment. Rivers comprise the major conduit for plastic transport with their deltas, estuaries and coastal lagoons being the key interface between lotic aquatic and the oceanic environment. However, we have very little knowledge of the role of coastal lagoons in the plastic pollution pathways. We present the spatial and temporal distribution and abundance of microplastics in sediments from two coastal lagoons in Ghana, West Africa. Sediment cores were taken from Mukwei Lagoon, Kpeshie Lagoon and from the mangroves at Kpeshie Lagoon; areas approximately 5-15km East from the centre of Accra. Microplastics were detected in all samples with a decreasing trend recorded from West to East. All three sites recorded a similar depth profile for plastics: after an initial increase from the surface samples, there was a significant decrease in microplastic concentrations with depth.Academy of Medical Science
Microplastic ingestion by pelagic and demersal fish species from the Eastern Central Atlantic Ocean, off the Coast of Ghana
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordWe present data on the occurrence of microplastics in fish from the Guinea current region off Ghana's Coast. Frequency of occurrence of microplastics in the fish species followed the order: Sardinella maderensis (41%) > Dentex angolensis > (33%) > Sardinella aurita (26%). Mean numbers of microplastics ingested were 40.0 ± 3.8, 32.0 ± 2.7 and 25.7 ± 1.6 for S. maderensis, D. angolensis and S. aurita respectively. Industrially produced pellets were the most dominant (31%) microplastic type followed by microbeads (29%), burnt film plastics (22%) and unidentified fragments (9%). Microfibers (2%), threads (2%) and foams (1 and below 1 for S. maderensis. The findings of the study show the common occurrence of microplastics in fish stocks and pave the way for future studies on microplastics in this Region.Academy of Medical Science
Jet formation and jet velocity fluctuations in a flue organ pipe
Flow visualization of the initial transient in a small recorderlike flue organ pipe is presented and the various stages of the jet formation are related to measurements of the acoustic response of the pipe. An initial acoustic signal, due to the unsteady volume flow of the jet, appears before the forming jet reaches the labium. This signal can easily be modeled using a low-frequency approximation. The initial trajectory of the jet makes a curve towards the exterior of the pipe. Under certain conditions, the jet may even, at first, miss the labium. This effect is related to the steepness of the pressure rise in the foot of the pipe. The initial impact of the jet with the labium appears to be a crucial factor in the triggering of the transient. Moving the labium towards the exterior of the pipe, using a steep pressure rise or putting ears around the mouth increase the chance that the jet will hit the labium. This initial impact is followed by an impulsive vortex shedding at the labium and subsequently a high-frequency varicoselike oscillation is observed on the jet. This oscillation is also observed without labium. After about three periods of the fundamental mode of the pipe, turbulence appears therefore destroying these coherent structures. Whereas the time dependency of the jet velocity dominates the first stage of the starting transient, the jet velocity fluctuations during steady-state result in a non-negligible damping. This loss mechanism is, for the fundamental mode of our experimental organ pipe, of the same order of magnitude as the radiation or visco-thermal damping.</p
The levels of Brachyspira hyodysenteriae binding to porcine colonic mucins differ between individuals, and binding is increased to mucins from infected pigs with de novo MUC5AC synthesis
Brachyspira hyodysenteriae colonizes the pig colon, resulting in mucohemorrhagic diarrhea and growth retardation. Fecal mucus is a characteristic feature of swine dysentery; therefore, we investigated how the mucin environment changes in the colon during infection with B. hyodysenteriae and how these changes affect this bacterium's interaction with mucins. We isolated and characterized mucins, the main component of mucus, from the colon of experimentally inoculated and control pigs and investigated B. hyodysenteriae binding to these mucins. Fluorescence microscopy revealed a massive mucus induction and disorganized mucus structure in the colon of pigs with swine dysentery. Quantitative PCR (qPCR) and antibody detection demonstrated that the mucus composition of pigs with swine dysentery was characterized by de novo expression of MUC5AC and increased expression of MUC2 in the colon. Mucins from the colon of inoculated and control pigs were isolated by two steps of isopycnic density gradient centrifugation. The mucin densities of control and inoculated pigs were similar, whereas the mucin quantity was 5-fold higher during infection. The level of B. hyodysenteriae binding to mucins differed between pigs, and there was increased binding to soluble mucins isolated from pigs with swine dysentery. The ability of B. hyodysenteriae to bind, measured in relation to the total mucin contents of mucus in sick versus healthy pigs, increased 7-fold during infection. Together, the results indicate that B. hyodysenteriae binds to carbohydrate structures on the mucins as these differ between individuals. Furthermore, B. hyodysenteriae infection induces changes to the mucus niche which substantially increase the amount of B. hyodysenteriae binding sites in the mucus
New planetary systems from the CalanâHertfordshire Extrasolar Planet Search
We report the discovery of eight new giant planets, and updated orbits for four known planets, orbiting dwarf and subgiant stars using the CORALIE, HARPS, and MIKE instruments as part of the CalanâHertfordshire Extrasolar Planet Search. The planets have masses in the range 1.1â5.4 MJâs, orbital periods from 40 to 2900 d, and eccentricities from 0.0 to 0.6. They include a double-planet system orbiting the most massive star in our sample (HD147873), two eccentric giant planets (HD128356b and HD154672b), and a rare 14 Herculis analogue (HD224538b). We highlight some population correlations from the sample of radial velocity detected planets orbiting nearby stars, including the mass function exponential distribution, confirmation of the growing body of evidence that low-mass planets tend to be found orbiting more metal-poor stars than giant planets, and a possible periodâmetallicity correlation for planets with masses >0.1 MJ, based on a metallicity difference of 0.16 dex between the population of planets with orbital periods less than 100 d and those with orbital periods greater than 100 d
Weakly haemolytic variants of Brachyspira hyodysenteriae newly emerged in Europe belong to a distinct subclade with unique genetic properties.
Brachyspira (B.) hyodysenteriae is widespread globally, and can cause mucohaemorrhagic colitis (swine dysentery, SD) with severe economic impact in infected herds. Typical strains of B. hyodysenteriae are strongly haemolytic on blood agar, and the haemolytic activity is believed to contribute to virulence in vivo. However, recently there have been reports of atypical weakly haemolytic isolates of B. hyodysenteriae (whBh). In this study, 34 European whBh and 82 strongly haemolytic isolates were subjected to comparative genomic analysis. A phylogenetic tree constructed using core single nucleotide polymorphisms showed that the whBh formed a distinct sub-clade. All eight genes previously associated with haemolysis in B. hyodysenteriae were present in the whBh. No consistent patterns of amino acid substitutions for all whBh were found in these genes. In contrast, a genome region containing six coding sequences (CDSs) had consistent nucleotide sequence differences between strongly and whBh isolates. Two CDSs were predicted to encode ABC transporter proteins, and a TolC family protein, which may have a role in the export of haemolysins from B. hyodysenteriae. Another difference in this region was the presence of three CDSs in whBh that are pseudogenes in strongly haemolytic isolates. One of the intact CDSs from whBh encoded a predicted PadR-like transcriptional repressor that may play a role in repression of haemolysis functions. In summary, a sub-clade of whBh isolates has emerged in Europe, and several genomic differences, that potentially explain the weakly haemolytic phenotype, were identified. These markers may provide targets for discriminatory molecular tests needed in SD surveillance
Novel health monitoring technology for inâservice diagnostics of intake separation in aircraft engines
Improved signal detection algorithms for unevenly sampled data. Six signals in the radial velocity data for GJ876
Human health risk and food safety implications of microplastic consumption by fish from coastal waters of the eastern equatorial Atlantic Ocean
Data availability:
Data will be made available on request.We examined the gastro-intestinal tracts (guts) of 160 fish species obtained from Nigerian coastal waters for microplastics and estimated annual microplastic intake by adult human population in the region from the fish species. A total of 5744 microplastics were recovered from the fish species analyzed with an average of 39.65 ± 5.67 items/individual. Microbeads (43%) occurred in all guts assessed, followed by fragments (27%), burnt film (14%), thread (9%), fibers (4%), and pellets (3%). Most microplastics recovered were below 1000 Όm with the least size being 85 Όm. Based on the size classes estimated for this study, we argue based on literature that close to 15% (i.e., >100 Όm) of the microplastics in the guts studied have the potential to translocate gut barriers of the fish species into muscles, where they get ingested by humans, and thereon get translocated to other human organ tissues. The estimated annual intake of microplastics from the consumption of whole fish by the adult population followed the trend; M. cephalus (178,220) > I. Africana (131,670) > P. senegalensis (115,710) > P. jubelini (109,060) > S. maderensis (101,080) > G. decadactylus (101,346) > S. melanotheron (65,170). Estimated annual intakes were generally higher for fish species with broad habitat and feeding preferences.Global Challenges Research Fund (GCRF), U
Neutrophil elastase and interleukin 17 expressed in the pig colon during Brachyspira hyodysenteriae infection synergistically with the pathogen induce increased mucus transport speed and production via mitogen-activated protein kinase 3
Brachyspira hyodysenteriae colonizes the pig colon, resulting in mucoid hemorrhagic diarrhea and mucus layer changes. These changes are characterized by a disorganized mucus structure and massive mucus induction with de novo expression of MUC5AC and increased production of MUC2. To investigate the mechanisms behind this altered mucin environment, we quantified the mRNA levels of mucin pathway genes and factors from the immune system in the colons of infected and control pigs and observed upregulation of neutrophil elastase, SPDEF, FOXA3, MAPK3/ ERK1, IL-17A, IL-1 beta, IL-6, and IL-8 expression. In vitro, colonic mucus-producing mucosal surfaces were treated with these factors along with B. hyodysenteriae infection and analyzed for their effect on mucin production. Neutrophil elastase and infection synergistically induced mucus production and transport speed, and interleukin 17A (IL-17A) also had similar effects, in both the presence and absence of infection. A mitogen-activated protein kinase 3 (MAPK3)/extracellular signal-regulated kinase 1 (ERK1) inhibitor suppressed these effects. Therefore, we suggest that the SPDEF, FOXA3, and MAPK3/ERK1 signaling pathways are behind the transcriptional program regulating mucin biosynthesis in the colon during B. hyodysenteriae infection. In addition to furthering the knowledge on this economically important disease, this mechanism may be useful for the development of therapies aimed at conditions where enhancing mucus production may be beneficial, such as chronic inflammatory disorders of the colon