Characterization and optimization of lipase activity produced by Pseudomonas monteilli 2403-KY120354 isolated from ground beef

A total of 56 Gram negative bacterial isolates were recovered from twenty ground beef samples and were screened for their potentiality to produce lipase. Forty four bacterial isolates were recorded as positive producers for lipase on tween as carbon source in solid medium. Also, the highly producer isolates were screened for lipase activity in submerged culture using olive oil as carbon and the most active isolate was 2043 which gave an activity of 20.0 ± 0.29 U/ml. The bacterial isolate 2403 was identified phenotypically according to Bergey’s Manual and genotypically using 16S rRNA genes analysis as Pseudomonas monteilli. Effect of some different factors on lipase activity were studied and the maximum lipase activity was achieved at reaction medium of pH 6 and incubated at 40°C for 60 min. Also, addition of Ba2+ in the reaction medium enhanced the lipase activity, while the other tested metals reduced the enzyme activity.Key words: Food contamination, lipase activity, olive oil, cultural conditions, Pseudomonas

Sex difference and intra-operative tidal volume: Insights from the LAS VEGAS study

BACKGROUND: One key element of lung-protective ventilation is the use of a low tidal volume (VT). A sex difference in use of low tidal volume ventilation (LTVV) has been described in critically ill ICU patients.OBJECTIVES: The aim of this study was to determine whether a sex difference in use of LTVV also exists in operating room patients, and if present what factors drive this difference.DESIGN, PATIENTS AND SETTING: This is a posthoc analysis of LAS VEGAS, a 1-week worldwide observational study in adults requiring intra-operative ventilation during general anaesthesia for surgery in 146 hospitals in 29 countries.MAIN OUTCOME MEASURES: Women and men were compared with respect to use of LTVV, defined as VT of 8 ml kg-1 or less predicted bodyweight (PBW). A VT was deemed 'default' if the set VT was a round number. A mediation analysis assessed which factors may explain the sex difference in use of LTVV during intra-operative ventilation.RESULTS: This analysis includes 9864 patients, of whom 5425 (55%) were women. A default VT was often set, both in women and men; mode VT was 500 ml. Median [IQR] VT was higher in women than in men (8.6 [7.7 to 9.6] vs. 7.6 [6.8 to 8.4] ml kg-1 PBW, P < 0.001). Compared with men, women were twice as likely not to receive LTVV [68.8 vs. 36.0%; relative risk ratio 2.1 (95% CI 1.9 to 2.1), P < 0.001]. In the mediation analysis, patients' height and actual body weight (ABW) explained 81 and 18% of the sex difference in use of LTVV, respectively; it was not explained by the use of a default VT.CONCLUSION: In this worldwide cohort of patients receiving intra-operative ventilation during general anaesthesia for surgery, women received a higher VT than men during intra-operative ventilation. The risk for a female not to receive LTVV during surgery was double that of males. Height and ABW were the two mediators of the sex difference in use of LTVV.TRIAL REGISTRATION: The study was registered at Clinicaltrials.gov, NCT01601223

La reconstrucción virtual de sitios patrimoniales en Luxor mediante la tecnología de la realidad aumentada

The research aims to investigate the virtual reconstruction of historical sites in Luxor through augmented reality technology. To achieve that, this research employed a method of descriptive-analytical methodology by using a questionnaire tool. Where a questionnaire was prepared and distributed to a random sample of 436 visitors to Luxor, whether foreigners or Egyptians. The results of the tools were analyzed using descriptive statistics, reliability analysis, coefficient analysis, and Pearson correlation analysis with the support of SPSS 25.0. The research results show a very strong positive correlation between the use of augmented reality at historical sites and the future of augmented reality at historical sites. The research recommended that the Ministry of Tourism and Antiquities pay more attention to the studies and research that concern the usage of new technologies in cultural heritage sites.  Keywords: Historical sites, Augmented Reality, reconstruction, temple of Amenhotep III, Luxor. Este trabajo ha tenido como objetivo investigar la reconstrucción virtual de yacimientos arqueológicos en Luxor a través de tecnología de realidad aumentada.  Para lograrlo, esta investigación empleó un sistema de metodología analítica descriptiva mediante el uso de un cuestionario que se elaboró y distribuyó a una muestra aleatoria de 436 visitantes de Luxor, ya fueran extranjeros o egipcios.  Los resultados de este cuestionario se analizaron mediante estadística descriptiva, análisis de fiabilidad, análisis de coeficientes y análisis de correlación de Pearson con el apoyo de SPSS 25.0.  Los resultados de la investigación muestran una correlación positiva muy fuerte entre el uso de la realidad aumentada y el futuro de la misma en yacimientos arqueológicos.  Esta investigación recomendó que el Ministerio de Turismo y Antigüedades egipcio preste más atención a los estudios e investigaciones que se refieren al uso de nuevas tecnologías en los lugares Patrimonio de la Humanidad. Palabras claves: Sitios históricos, Realidad Aumentada, reconstrucción virtual, templo de Amenhotep III, Luxor

The behavior of 2-chloroquinoline-3-carbaldehyde and 2-oxoquinoline-3-carbaldehyde toward stabilized methylenetriphenylphosphoranes and secondary amines

<p>In this study use was made of the Wittig carbonyl olefination reaction and stereo-identification of the resulting alkenes. Condensation of 2-chloroquinoline-3-carbaldehyde with some selected stabilized phosphonium ylides yielded a mixture of the corresponding E and Z olefins in each case. On the other hand reaction of 2-oxoquinoline-3-carbaldehyde with the selected ylides afforded the respective olefins only in one of the possible stereoisomers. The reaction of 2-chloroquinoline-3-carbaldehyde with acetylmethylenetriphenylphosphorane produced the respective olefine together with acridin-3-ol. Heating of (E) and/or (Z)-methyl 3-(2-chloroquinolin-3-yl)acrylate with hydrazine hydrate yielded the corresponding propan-1-ol derivative. Dechlorination of (E)-ethyl 3-(2-chloroquinolin-3-yl)acrylate and/or (E)-4-(2-chloroquinolin-3-yl)but-3-en-2-one was effected upon treatment with morpholine or piperidine in absolute ethanol to give the respective enone derivatives.</p

Association between night-time surgery and occurrence of intraoperative adverse events and postoperative pulmonary complications

Background: The aim of this post hoc analysis of a large cohort study was to evaluate the association between night-time surgery and the occurrence of intraoperative adverse events (AEs) and postoperative pulmonary complications (PPCs). Methods: LAS VEGAS (Local Assessment of Ventilatory Management During General Anesthesia for Surgery) was a prospective international 1-week study that enrolled adult patients undergoing surgical procedures with general anaesthesia and mechanical ventilation in 146 hospitals across 29 countries. Surgeries were defined as occurring during 'daytime' when induction of anaesthesia was between 8: 00 AM and 7: 59 PM, and as 'night-time' when induction was between 8: 00 PM and 7: 59 AM. Results: Of 9861 included patients, 555 (5.6%) underwent surgery during night-time. The proportion of patients who developed intraoperative AEs was higher during night-time surgery in unmatched (43.6% vs 34.1%; P<0.001) and propensity-matched analyses (43.7% vs 36.8%; P = 0.029). PPCs also occurred more often in patients who underwent night-time surgery (14% vs 10%; P = 0.004) in an unmatched cohort analysis, although not in a propensity-matched analysis (13.8% vs 11.8%; P = 0.39). In a multivariable regression model, including patient characteristics and types of surgery and anaesthesia, night-time surgery was independently associated with a higher incidence of intraoperative AEs (odds ratio: 1.44; 95% confidence interval: 1.09-1.90; P = 0.01), but not with a higher incidence of PPCs (odds ratio: 1.32; 95% confidence interval: 0.89-1.90; P = 0.15). Conclusions: Intraoperative adverse events and postoperative pulmonary complications occurred more often in patients undergoing night-time surgery. Imbalances in patients' clinical characteristics, types of surgery, and intraoperative management at night-time partially explained the higher incidence of postoperative pulmonary complications, but not the higher incidence of adverse events

Intraoperative ventilator settings and their association with postoperative pulmonary complications in neurosurgical patients: post-hoc analysis of LAS VEGAS study

Background: Limited information is available regarding intraoperative ventilator settings and the incidence of postoperative pulmonary complications (PPCs) in patients undergoing neurosurgical procedures. The aim of this post-hoc analysis of the 'Multicentre Local ASsessment of VEntilatory management during General Anaesthesia for Surgery' (LAS VEGAS) study was to examine the ventilator settings of patients undergoing neurosurgical procedures, and to explore the association between perioperative variables and the development of PPCs in neurosurgical patients. Methods: Post-hoc analysis of LAS VEGAS study, restricted to patients undergoing neurosurgery. Patients were stratified into groups based on the type of surgery (brain and spine), the occurrence of PPCs and the assess respiratory risk in surgical patients in Catalonia (ARISCAT) score risk for PPCs. Results: Seven hundred eighty-four patients were included in the analysis; 408 patients (52%) underwent spine surgery and 376 patients (48%) brain surgery. Median tidal volume (VT) was 8 ml [Interquartile Range, IQR = 7.3-9] per predicted body weight; median positive end-expiratory pressure (PEEP) was 5 [3 to 5] cmH20. Planned recruitment manoeuvres were used in the 6.9% of patients. No differences in ventilator settings were found among the sub-groups. PPCs occurred in 81 patients (10.3%). Duration of anaesthesia (odds ratio, 1.295 [95% confidence interval 1.067 to 1.572]; p = 0.009) and higher age for the brain group (odds ratio, 0.000 [0.000 to 0.189]; p = 0.031), but not intraoperative ventilator settings were independently associated with development of PPCs. Conclusions: Neurosurgical patients are ventilated with low VT and low PEEP, while recruitment manoeuvres are seldom applied. Intraoperative ventilator settings are not associated with PPCs