Biochemical responses of cucumber to Tetranychus urticae Koch (Acari: Tetranychidae) mediated biotic stress

The effect of two spotted spider mite (Tetranychus urticae Koch) feeding on leaf level physiological characteristics of cucumber (Cucumis sativus Linnaeus) was investigated. Young cucumber plants were artificially infested with different densities of T. urticae (5, 10, 15 and 20 mites/ grown up leaf) while uninfested plants acted as control. Post infestation, the plants differed in their support to mite density in accordance with initial infestation density and observation period. Highly significant negative correlation of -0.92, -0.93, -0.95 and -0.92 for total chlorophyll, chlorophyll a, chlorophyll b and carotenoids, respectively, at the highest infestation level) was recorded between mite density and photosynthetic pigments in infested leaves as compared to uninfested ones. There was a significant decrease (P= 0.05) in the level of (a progressive decline from 2.82, 0.36 and 2.17% dry weight in control to the maximum of 2.09, 0.26 and 1.87% dry weight for N, P and K, respectively, at highest infestation level) in the infested leaves in response to mite infestation. Interaction between initial infestation level and observation period also suggested a significant impact of T. urticae infestation on the leaf phytochemicals of cucumber (P= 0.05)

Pleiotropic morphological and abiotic stress resistance phenotypes of the hyper-abscisic acid producing Abo<SUP>&#8722;</SUP> mutant in the periwinkle Catharanthus roseus

The pleiotropic properties of aabo abo (Abo&#8722;) &#947;-ray induced mutant ofCatharanthus roseuscv. Nirmal, selected among the M2 generation seeds for ability to germinate at 45&#176;C, are described. The mutant produced seeds possessing tricotyledonous embryos, unlike the typically dicotyledonous embryos present in the wild type Abo+ seeds. In comparison to Abo+ adults, the mutant plants had short stature and lanceolate leaves. The vascular bundles in the leaves and stem were poorly developed. Leaf surfaces were highly trichomatous, epidermal, cortex and mesophyll cells were small sized and a large majority of stomata were closed. Besides high temperature, the mutant was salinity and water-stress tolerant. The abscisic acid (ABA) content in the leaves was about 500-fold higher. The genetic lesionabo responsible for the above pleiotropy was recessive and inherited in Mendelian fashion. The seedlings and adult plants of the mutant accumulated higher proline than Abo+ plants. The phenotypes ofabo abo mutants permitted the conclusions that (i) the mutant synthesizes ABA constitutively, (ii) both ABA-dependent and ABA independent pathways for proline and betaine accumulation are functional in the mutant, and (iii) cell division, elongation and differentiation processes in embryo and adult plant stages are affected in the mutant

Activities of sucrose to starch metabolizing enzymes during grain filling in late sown wheat under water stress

Tolerance to water deficit in relation to activities of sucrose-to- starch metabolizing enzymes and starch accumulation was studied in the grains of contrasting wheat (Triticum aestivum L.) genotypes (WH1021 and WH1080; tolerant) and (WH711 and HD2687; susceptible) under late planting conditions. The activities of starch metabolizing enzymes i.e. sucrose synthase (SuSase), ADP-glucose pyrophosphorylase (AGPase), soluble starch synthase (SSS) and starch branching enzymes (SBE) were substantially enhanced by water deficit in all genotypes at early to mid-grain filling stage showing peaks at 14 to 21 days after anthesis (DAA); while decreased significantly at mid-late grain filling stage with maximum decline at 35 DAA. Activities of all the enzymes under study showed maximum decline in activity (28.4–60%) in susceptible genotype WH711; whereas WH1021 proved to be most tolerant one with minimum decline in enzyme activity (14.9–32.8%). Starch content was also markedly reduced (21%) in WH711 due to drought while WH1021 reported 12% decline corresponding well with enzyme activity. A faster pre-mature cessation of starch deposition occurred in susceptible wheat genotypes compared to tolerant ones. A significant and positive correlation of the enzyme activities with starch accumulation (r = 0.491–0.555 at P0.05 for SuSase, AGPase, SSS and r = 0.638 at P0.01 for SBE) under well watered conditions indicated that enhancing the activities of the enzymes would lead to increase in starch accumulation and thus faster grain filling. Genotype WH1021 proved to be most efficient based on comparatively higher enzyme activity and least yield penalty under late planting conditions combined with water scarcity

Effects of different rates of nitrogen and pinching on yield and yield attributes of African marigold (Tagetes erecta L.)

Proper pinching practice and the optimum rate of nitrogen (N) enhance the production of marigold. An experiment was conducted at a farmer’s field in Gadawa-4, Gangaparaspur, Dang, Nepal from July 2018 to November 2018 to investigate the effects of different rates of nitrogen and pinching on yield and yield attributes of African marigold (cv. Kolkata Local). Two factorial experiment was laid in the Randomized Complete Block Design (RCBD) with three replications. There were eight treatments, consist of four rates of nitrogen (0, 50, 100 and 150 kg/ha) and two levels of pinching (pinching and non-pinching). The maximum plant height (89.70 cm), the diameter of flower (4.29 cm) and the fresh weight per flower (4.32 g) and early days to 50% flowering (61.58) were obtained at non- pinching. The highest number of flowers (60.66), yield per plant (237.49 g) and yield per hectare (9.89 t/ha) were obtained with pinching. The highest plant height (92.20 cm) was recorded at 150 kg/ha of N but the highest yield per plant (238.18 g) and yield per ha (9.91 t/ha) was obtained at 50 kg/ha of nitrogen application. No significant effect was noted on the days to 50% flowering, number of flowers per plant, fresh weight per flower and diameter of flowers by different rates of nitrogen. The interaction of pinching and different rates of nitrogen showed non- significant effect on yield per plant and yield per ha. But, the combination of pinching and nitrogen rates at 50 kg/ha recorded the maximum yield per plant (249.20 g) and yield per ha (10.36 t/ha). Hence it is suggested to use pinching practice with optimum application of nitrogen @ 50 kg/ha to obtain high yield of marigold

Duplication of intrachromosomal insertion segments 4q32→q35 confirmed by comparative genomic hybridization and fluorescent in situ hybridization

A 35-year-old man with infertility was referred for chromosomal analysis. In routine cytogenetic analysis, the patient was seen to have additional material of unknown origin on the terminal region of the short arm of chromosome 4. To determine the origin of the unknown material, we carried out high-resolution banding, comparative genomic hybridization (CGH), and FISH. CGH showed a gain of signal on the region of 4q32→q35. FISH using whole chromosome painting and subtelomeric region probes for chromosome 4 confirmed the aberrant chromosome as an intrachromosomal insertion duplication of 4q32→q35. Duplication often leads to some phenotypic abnormalities; however, our patient showed an almost normal phenotype except for congenital dysfunction in spermatogenesis

Etiologic factors related to unsatisfactory ThinPrep cervical cytology: Evaluation and potential solutions to improve

BACKGROUND: In cervical cytology, the unsatisfactory rates for ThinPrep (TP) are slightly higher compared to SurePath. We examined various causes and explored potential for resolution of this discrepancy. MATERIALS AND METHODS: Totally, 19,422 cases were reviewed and 1000 unsatisfactory specimens were selected and analyzed. 531 specimens were available for wash protocol. Out of 114 unsatisfactory specimens associated with atrophic cellular changes (ACC), 48 were resubmitted by provider and reevaluated. RESULTS: Lubricant and lubricant-like debris/contamination (LUBE) was the most common cause of unsatisfactory specimens (68%; 681/1000) followed by blood (7.5%); ACC only (without other interfering factors) (2.4%); inflammation (3.0%); and combinations thereof (1.9%). 11.5% showed scant cellularity without an identifiable cause. 3.3% were virtually acellular. Wash protocol improved cellularity in 48% (256/531) of cases. However, only 29% (73/256) of those were satisfactory (with more than 5000 cells). Quantitative reduction in LUBE after wash protocol varied with different morphological subtypes. Interpretation patterns on satisfactory specimens after wash protocol were comparable to the results on selected cohort of specimens during the same study period. Out of 114 ACC, wash protocol was performed on 68 ACC specimens leading to satisfactory TP in 24% (16/68). Totally, 48 cases reported as unsatisfactory with ACC, were resubmitted by the providers between 2 weeks and 2 years. 44 (92%) showed increased cellularity, out of which 52% (23/44) did not show ACC. CONCLUSION: LUBE was the most common cause of unsatisfactory TP in addition to interference by blood and association with atrophic changes. Knowing the morphological spectrum of LUBE would help to identify it as the cause of unsatisfactory TP. Communicating the cause of unsatisfactory TP such as LUBE, ACC, and blood would hint the provider to take appropriate precaution during submission of the repeat specimen, leading to improved patient care

<span style="font-weight:normal; mso-bidi-font-weight:bold" lang="EN-US">Genetic variability among <i style="mso-bidi-font-style: normal">Jatropha</i> species as revealed by amplified fragment length polymorphism (AFLP) markers </span>

496-501There is a mounting interest in Jatropha species as a biodiesel tree to lessen the energy crisis and generate income in rural areas of developing countries. The present study was conducted on 23 accessions of Jatropha belonging to seven species, comprising 6 accessions of J. curcas and <i style="mso-bidi-font-style: normal">J. integerrima each, 4 accessions of <i style="mso-bidi-font-style: normal">J. gossypifolia, 2 accessions of <i style="mso-bidi-font-style: normal">J. dioca, J. podagrica, J. tanjorensis each and 1 accession of J. glandulifera. These different species were used to determine the genetic diversity based on amplified fragment length polymorphism (AFLP) markers. Four AFLP primer combinations produced a total of 369 fragments with an average of 92.25 fragments per primer combination. A total of 361 (97.54%) fragments showed polymorphism in the germplasm analyzed. In order to access the discriminatory power of four primer combination used, a variety of marker attributes like polymorphic information content (PIC), marker index (MI) and resolving power (RP) values were calculated. The PIC (0.331), RP (45.18) and MI (42.659) showed the efficiency of used primer combinations. The Jaccard’s similarity coefficient ranged from 0.165 to 0.993. Genotyping data obtained from all 361 polymorphic fragments were used to group the accessions using the UPGMA-phenogram and principal component analysis (PCA). Majority of group obtained in phenogram and PCA contained accessions as per their species. AFLP is reliable molecular technique and provides one of the most informative approaches to establish genetic relatedness in <i style="mso-bidi-font-style: normal">Jatropha, which may also be true for other related species

Efficient Group Mobility for Heterogeneous Sensor Networks

Abstract — Mobility management protocols allow wireless devices to move between networks. These protocols have traditionally supported the mobility of individual nodes and are therefore not optimized to support the migration of groups. Accordingly, the time required to re-establish connectivity, frequency of dropped packets and contention for the air interface increase significantly for mobile groups. We propose a protocol for mobile groups that reduces all of the above by allowing a single node to perform handoffs on behalf of all group members. This “gateway ” node eliminates the need for multiple handoff messages by obscuring group membership to external parties. Through extensive simulation and implementation, we show significant reduction in handoff times, message complexity and packet loss for groups of heterogeneous, mobile sensors running AODV and DSDV. By leveraging the naturally occurring hierarchy, we demonstrate that it is possible for groups to efficiently use traditional mobility protocols to support their collective movements