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Comparisons of host mitochondrial, nuclear and endosymbiont bacterial genes reveal cryptic fig wasp species and the effects of Wolbachia on host mtDNA evolution and diversity
Background
Figs and fig-pollinating wasp species usually display a highly specific one-to-one association. However, more and more studies have revealed that the "one-to-one" rule has been broken. Co-pollinators have been reported, but we do not yet know how they evolve. They may evolve from insect speciation induced or facilitated by Wolbachia which can manipulate host reproduction and induce reproductive isolation. In addition, Wolbachia can affect host mitochondrial DNA evolution, because of the linkage between Wolbachia and associated mitochondrial haplotypes, and thus confound host phylogeny based on mtDNA. Previous research has shown that fig wasps have the highest incidence of Wolbachia infection in all insect taxa, and Wolbachia may have great influence on fig wasp biology. Therefore, we look forward to understanding the influence of Wolbachia on mitochondrial DNA evolution and speciation in fig wasps.
Results
We surveyed 76 pollinator wasp specimens from nine Ficus microcarpa trees each growing at a different location in Hainan and Fujian Provinces, China. We found that all wasps were morphologically identified as Eupristina verticillata, but diverged into three clades with 4.22-5.28% mtDNA divergence and 2.29-20.72% nuclear gene divergence. We also found very strong concordance between E. verticillata clades and Wolbachia infection status, and the predicted effects of Wolbachia on both mtDNA diversity and evolution by decreasing mitochondrial haplotypes.
Conclusions
Our study reveals that the pollinating wasp E. verticillata on F. microcarpa has diverged into three cryptic species, and Wolbachia may have a role in this divergence. The results also indicate that Wolbachia strains infecting E. verticillata have likely resulted in selective sweeps on host mitochondrial DNA
Parents’ Beliefs about and Associations to their Elementary Children’s Home Technology Usage
This study sought to gather information through a survey of how newcomer parents’ beliefs about technology usage and how they engage with technology as they support their children with twenty-first century literacies. Parent respondents (N = 70) were drawn from two publicly funded schools in the Niagara Region, Ontario, Canada, where the population tends to be immigrant, visible minority, with post-secondary education, but unemployed and low income. Descriptive statistics quantified daily technology activities as being communication-oriented with the majority of parents holding distinct beliefs about the amount and type of their children’s technology usage. Chi-square tests indicated significant associations for demographic characteristics such as the gender, age, education, first language, and ethnicity of the parents as determinants of their beliefs about their children’s technology usage (e.g., social media, mobile phones, television). As well, levels of access and use varied in terms of the number of new technologies and the types of literacy practices that families engage in. Immigrant parents might hold misconceptions about twenty-first century literacies, therefore there should be an attempt to assist them to provide responsive twenty-first century literacy and technology support for their children
Exploiting solar visible-range observations by inversion techniques: from flows in the solar subsurface to a flaring atmosphere
Observations of the Sun in the visible spectral range belong to standard
measurements obtained by instruments both on the ground and in the space.
Nowadays, both nearly continuous full-disc observations with medium resolution
and dedicated campaigns of high spatial, spectral and/or temporal resolution
constitute a holy grail for studies that can capture (both) the long- and
short-term changes in the dynamics and energetics of the solar atmosphere.
Observations of photospheric spectral lines allow us to estimate not only the
intensity at small regions, but also various derived data products, such as the
Doppler velocity and/or the components of the magnetic field vector. We show
that these measurements contain not only direct information about the dynamics
of solar plasmas at the surface of the Sun but also imprints of regions below
and above it. Here, we discuss two examples: First, the local time-distance
helioseismology as a tool for plasma dynamic diagnostics in the near subsurface
and second, the determination of the solar atmosphere structure during flares.
The methodology in both cases involves the technique of inverse modelling.Comment: 29 pages, 15 figures. Accepted for publication in the book "Reviews
in Frontiers of Modern Astrophysics: From Space Debris to Cosmology" (eds
Kabath, Jones and Skarka; publisher Springer Nature) funded by the European
Union Erasmus+ Strategic Partnership grant "Per Aspera Ad Astra Simul"
2017-1-CZ01-KA203-03556
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress
In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the genes of secretion II system during copper stress suggests that this pathway may have an important role in the adaptative response of X. axonopodis pv. citri to toxic compounds. These findings highlight the potential role of these genes in attenuating the toxicity of certain metals and could represent an important means of bacterial resistance against chemicals used to control diseases
Identification of metabolic engineering targets through analysis of optimal and sub-optimal routes
Identification of optimal genetic manipulation strategies for redirecting substrate uptake towards a desired product is a challenging task owing to the complexity of metabolic networks, esp. in terms of large number of routes leading to the desired product. Algorithms that can exploit the whole range of optimal and suboptimal routes for product formation while respecting the biological objective of the cell are therefore much needed. Towards addressing this need, we here introduce the notion of structural flux, which is derived from the enumeration of all pathways in the metabolic network in question and accounts for the contribution towards a given biological objective function. We show that the theoretically estimated structural fluxes are good predictors of experimentally measured intra-cellular fluxes in two model organisms, namely, Escherichia coli and Saccharomyces cerevisiae. For a small number of fluxes for which the predictions were poor, the corresponding enzyme-coding transcripts were also found to be distinctly regulated, showing the ability of structural fluxes in capturing the underlying regulatory principles. Exploiting the observed correspondence between in vivo fluxes and structural fluxes, we propose an in silico metabolic engineering approach, iStruF, which enables the identification of gene deletion strategies that couple the cellular biological objective with the product flux while considering optimal as well as sub-optimal routes and their efficiency.This work was supported by the Portuguese Science Foundation [grant numbers MIT-Pt/BS-BB/0082/2008, SFRH/BPD/44180/2008 to ZS] (http://www.fct.pt/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
A study of a couple with type 2 diabetes: dyadic adjustment and psychological morbidity
Objective: this study assessed dyadic adjustment and psychological morbidity
in type 2 diabetic patients and their partners, focusing on the role of gender.
Methods: 214 diabetic patients and their partners participated in the cross-sectional
study and were assessed on psychological morbidity (HADS) and marital
adjustment (RDAS). Data was analyzed using dyadic analysis, a statistical process
that studies the patient/partner dyads simultaneously.
Results: results revealed that the negative relationship between dyadic adjustment
and psychological morbidity in female patients was stronger than in male
diabetic patients or in partners of male diabetic patients. On the other hand, the
relationship between dyadic adjustment and psychological morbidity in partners
of diabetic men was stronger than the same relationship in partners of diabetic
women.
Conclusion: since gender is a moderator, it is important to attend to the different
needs of female and male patients and the education of diabetic patients
should be centered on the patient/partner dyad.Fundação para a Ciência e a Tecnologia (FCT
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