The Na+/glucose cotransporters: from genes to therapy

Glucose enters eukaryotic cells via two types of membrane-associated carrier proteins, the Na+/glucose cotransporters (SGLT) and the facilitative glucose transporters (GLUT). The SGLT family consists of six members. Among them, the SGLT1 and SGLT2 proteins, encoded by the solute carrier genes SLC5A1 and SLC5A2, respectively, are believed to be the most important ones and have been extensively explored in studies focusing on glucose fluxes under both physiological and pathological conditions. This review considers the regulation of the expression of the SGLT promoted by protein kinases and transcription factors, as well as the alterations determined by diets of different compositions and by pathologies such as diabetes. It also considers congenital defects of sugar metabolism caused by aberrant expression of the SGLT1 in glucose-galactose malabsorption and the SGLT2 in familial renal glycosuria. Finally, it covers some pharmacological compounds that are being currently studied focusing on the interest of controlling glycemia by antagonizing SGLT in renal and intestinal tissues

Story Map Caminhos d’O Conspirador: um percurso literário na vila de Marvão com Branquinho da Fonseca

Os autores pretendem apresentar uma nova forma de divulgação da investigação em turismo literário, num formato mais atrativo, recorrendo às novas tecnologias e bem assim, aferir as suas potencialidades. A ferramenta tecnológica utilizada foi o story map que permite combinar mapas na Web com aplicações e templates que incorporam funções interativas e mapas dinâmicos. Um dos recursos utilizados na produção do story map foi a base de dados do Museu Virtual de Turismo (MUVITUR) em cujo site o produto final será disponibilizado. Os conteúdos e itinerários turísticos literários organizados por especialistas permitem envolver os viajantes online nas geografias imaginárias, para as quais os escritores nos remetem. Experiências virtuais mais envolventes possibilitam a cada utilizador a descoberta dos melhores percursos por territórios literários. O objeto do presente trabalho foi o conto “O Conspirador”, de Branquinho da Fonseca (1905-1974) tendo o percurso na vila de Marvão sido enriquecido com desenhos e fotografias da sua autoriaThe authors intend to present a more attractive format to disseminate research in literary tourism, making use of new technologies. The story map is the technological tool used, and it allows the combination of online maps with apps and templates that incorporate interactive functions and dynamic maps. One of the resources the authors use in the production of the story map is the database of the Virtual Tourism Museum (MUVITUR) on whose website the final product will be made available. The literary content and tourist itineraries organized by specialists allow online travellers to be involved in imaginary geographies created by writers. More engaging virtual experiences enable users to discover the best routes through literary territories. The object of the present study was the short story “O Conspirador”, by Branquinho da Fonseca (1905-1974) and the itinerary in the village of Marvão was enhanced by drawings and photographs authored by the writer.info:eu-repo/semantics/publishedVersio

Innovative moments and poor outcome in narrative therapy

To analyse a poor outcome case of narrative therapy with a woman victim of intimate violence. Method: The Innovative Moments Coding System: version 1 was applied to all sessions to track the innovative moments (i-moments) in the therapeutic process. I moments are the narrative details that occur in psychotherapeutic conversations that are outside the influence of the problematic narrative. This research aims to describe the processes involved in the stability of meanings in psychotherapy through a dialogical approach to meaning making. Findings: Contrarily to what usually occurs in good outcome cases, re-conceptualization i-moments are absent. Moreover, two specific types of i-moments emerged with higher duration: reflection and protest. Qualitative analysis showed that the potential meanings of these i-moments were surpassed by a return to the problematic narrative. Conclusion: The therapeutic stability seems to be maintained by a systematic return to the problematic narrative after the emergence of novelties. This process was referred from a dialogical perspective as a mutual in-feeding of voices, one that emerges in the i-moment and another one that supports the problematic narrative, which is maintained by an oscillation between these two types of voices during therapy.This article was supported by the Portuguese Foundation for Science and Technology (FCT), by the Grant PTDC/PSI/72846/2006 (Narrative Processes in Psychotherapy, 2007-2010) and by the PhD Grant SFRH/BD/16995/2004

Application Of The Method Of Continued Fractions To Multichannel Studies On Electronic Excitation Of H2 By Electron Impact

In the present work, the method of continued fractions at a five-channel close-coupling level of approximation is applied to study the low-energy electron-impact excitation in linear molecules. Particularly, cross sections for the X1Σg +→b3Σ u +,X1Σg +→a3Σg +, and X1Σg +→c3Πu transitions in H2 in the (15-40)-eV energy range are reported. As in our early two-state studies, no orthogonality constraint between the bound and continuum orbitals is imposed and the one-electron exchange terms are considered explicitly. In general, our calculated cross sections are in good agreement with the results obtained by the four-state Kohn variational method. Comparison between our calculated results with available experimental data is encouraging. ©2001 The American Physical Society.63316Pitchford, L., McKoy, V., Chutjian, A., Trajmar, S., (1986) Proceedings of the Meeting of the Fourth International Swarm and the Inelastic Electron-Molecule Collisions Symposium, , Swarm Studies and Inelastic Electron-Molecule Collisions, edited by L. Pitchford, V. McKoy, A. Chutjian, and S. Trajmar Springer-Verlag, New YorkBaluja, K.L., Noble, C.J., Tennyson, J., (1985) J. Phys. B, 18, pp. L851Schneider, B.I., Collins, L.A., (1985) J. Phys. B, 18, pp. L857Lima, M.A.P., Gibson, T.L., Huo, W.M., McKoy, V., (1985) J. Phys. B, 18, pp. L865Pritchard, H.P., McKoy, V., Lima, M.A.P., (1990) Phys. Rev. A, 41, p. 546Rescigno, T.N., Schneider, B.I., (1992) Phys. Rev. A, 45, p. 2894Sun, Q., Winstead, C., McKoy, V., Lima, M.A.P., (1992) J. Chem. Phys., 96, p. 3531Lee, M.-T., Fujimoto, M.M., Kroin, T., Iga, I., (1996) J. Phys. B, 29, pp. L425Lee, M.-T., Fujimoto, M.M., Iga, I., (1998) J. Mol. Struct.: THEOCHEM, 432, p. 197Branchett, S.E., Tennyson, J., Morgan, L.A., (1990) J. Phys. B, 23, p. 4625Branchett, S.E., Tennyson, J., Morgan, L.A., (1991) J. Phys. B, 24, p. 3479Parker, S.D., McCurdy, C.W., Rescigno, T.N., Lengsfield III, B.H., (1991) Phys. Rev. A, 43, p. 3514Sartori, C.S., Lima, M.A.P., (1995) Scientific Program and Abstract of Contributed Papers, XIX ICPEAC, p. 28. , edited by J. B. A. Mitchell, J. W. McConkey, and C. E. Brion Whistler, CanadaLee, M.-T., Iga, I., Fujimoto, M.M., Lara, O., (1995) J. Phys. B, 28, pp. L299Lee, M.-T., Iga, I., Fujimoto, M.M., Lara, O., (1995) J. Phys. B, 28, p. 3325Horacek, J., Sasakawa, T., (1983) Phys. Rev. A, 28, p. 2151Horacek, J., Sasakawa, T., (1984) Phys. Rev. A, 30, p. 2274Fano, U., Dill, D., (1972) Phys. Rev. A, 6, p. 185Fliflet, A.W., McKoy, V., (1980) Phys. Rev. A, 21, p. 1863Goddard III, W.A., Hunt, W.J., (1974) Chem. Phys. Lett., 24, p. 464Huzinaga, S., (1965) J. Chem. Phys., 42, p. 1293Kolos, W., Roothaan, C.C.J., (1960) Rev. Mod. Phys., 32, p. 219Lima, M.A.P., Gibson, T.L., McKoy, V., Huo, W.M., (1988) Phys. Rev. A, 38, p. 4527Khakoo, M.A., Trajmar, S., (1986) Phys. Rev. A, 34, p. 146Nishimura, H., Danjo, A., (1986) J. Phys. Soc. Jpn., 55, p. 303

The effects of an intronic polymorphism in TOMM40 and APOE genotypes in sporadic inclusion body myositis.

A previous study showed that, in carriers of the apolipoprotein E (APOE) genotype ε3/ε3 or ε3/ε4, the presence of a very long (VL) polyT repeat allele in "translocase of outer mitochondrial membrane 40" (TOMM40) was less frequent in patients with sporadic inclusion body myositis (sIBM) compared with controls and associated with a later age of sIBM symptom onset, suggesting a protective effect of this haplotype. To further investigate the influence of these genetic factors in sIBM, we analyzed a large sIBM cohort of 158 cases as part of an International sIBM Genetics Study. No significant association was found between APOE or TOMM40 genotypes and the risk of developing sIBM. We found that the presence of at least 1 VL polyT repeat allele in TOMM40 was significantly associated with about 4 years later onset of sIBM symptoms. The age of onset was delayed by 5 years when the patients were also carriers of the APOE genotype ε3/ε3. In addition, males were likely to have a later age of onset than females. Therefore, the TOMM40 VL polyT repeat, although not influencing disease susceptibility, has a disease-modifying effect on sIBM, which can be enhanced by the APOE genotype ε3/ε3

Expression of E-cadherin, Snail and Hakai in epithelial cells isolated from the primary tumor and from peritumoral tissue of invasive ductal breast carcinomas

Epithelial intercellular cohesion, mainly mediated by E-cadherin (CDH1) expression and function, may be deregulated during cancer cell invasion of adjacent tissues and lymphatic and vascular channels. CDH1 expression is down-modulated in invasive lobular breast carcinomas but its regulation in invasive ductal carcinomas (IDC) is less clear. CDH1 expression is repressed by transcription factors such as Snail (SNAI1) and its product is degraded after Hakai ubiquitination. We compared CDH1, SNAI1 and HAKAI mRNA expression in IDC and paired adjacent normal breast tissue and evaluated its relation with node metastasis and circulating tumor cells. Matched tumor/peritumoral and blood samples were collected from 30 patients with early IDC. Epithelial cells from each compartment (tumor/peritumoral) were recovered by an immunomagnetic method and gene expression was determined by real time RT-PCR. There were no differences in CDH1, SNAI1 and HAKAI mRNA expression between tumor and corresponding peritumoral samples and no differential tumoral gene expression according to nodal involvement. Another 30 patients with a long-term follow-up (at least 5 years) and a differential prognosis (good or poor, as defined by breast cancer death) had E-cadherin and Snail protein detected by immunohistochemistry in tumor samples. In this group, E-cadherin-positive expression, but not Snail, may be associated with a better prognosis. This is the first report simultaneously analyzing CDH1, SNAI1 and HAKAI mRNA expression in matched tumor and peritumoral samples from patients with IDC. However, no clear pattern of their expression could distinguish the invasive tumor compartment from its adjacent normal tissue.FAPESPCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Clinical outcomes of patients with acute myeloid leukemia: evaluation of genetic and molecular findings in a real-life setting

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Hard diffractive quarkonium hadroproduction at high energies

We present a study of heavy quarkonium production in hard diffractive process by the Pomeron exchange for Tevatron and LHC energies. The numerical results are computed using recent experimental determination of the diffractive parton density functions in Pomeron and are corrected by unitarity corrections through gap survival probability factor. We give predictions for single as well as central diffractive ratios. These processes are sensitive to the gluon content of the Pomeron at small Bjorken-x and may be particularly useful in studying the small-x physics. They may also be a good place to test the different available mechanisms for quarkonium production at hadron colliders.Comment: 7 pages, 3 figures, 1 table. Final version to be published in European Physical Journal