The influence of bile acids homeostasis by cryptotanshinone-containing herbs

Background: Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids. Aim: The present study aims to investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids. Methods: The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS. Results: Initial screening showed that 100 μM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuronidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour. Conclusion: All these data indicated the possibility of cryptotanshinone’s influence towards the bile acids metabolism and homeostasis of bile acids.Keywords: herbs, lithocholic acid (LCA), homeostasisAfrican Health sciences Vol 14 No. 1 March 201

ProRes: Exploring Degradation-aware Visual Prompt for Universal Image Restoration

Image restoration aims to reconstruct degraded images, e.g., denoising or deblurring. Existing works focus on designing task-specific methods and there are inadequate attempts at universal methods. However, simply unifying multiple tasks into one universal architecture suffers from uncontrollable and undesired predictions. To address those issues, we explore prompt learning in universal architectures for image restoration tasks. In this paper, we present Degradation-aware Visual Prompts, which encode various types of image degradation, e.g., noise and blur, into unified visual prompts. These degradation-aware prompts provide control over image processing and allow weighted combinations for customized image restoration. We then leverage degradation-aware visual prompts to establish a controllable and universal model for image restoration, called ProRes, which is applicable to an extensive range of image restoration tasks. ProRes leverages the vanilla Vision Transformer (ViT) without any task-specific designs. Furthermore, the pre-trained ProRes can easily adapt to new tasks through efficient prompt tuning with only a few images. Without bells and whistles, ProRes achieves competitive performance compared to task-specific methods and experiments can demonstrate its ability for controllable restoration and adaptation for new tasks. The code and models will be released in \url{https://github.com/leonmakise/ProRes}

The influence of bile acids homeostasis by cryptotanshinone-containing herbs

Background: Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids. Objective:To investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids. Methods:The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS. Results:Initial screening showed that 100 μM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuro-nidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour. Conclusion: All these data indicated the possibility of cryptotanshinone’s influence towards bile acids metabolism and homeostasis of bile acids

Probe substrate and enzyme source-dependent inhibition of UDPglucuronosyltransferase (UGT) 1A9 by wogonin

Background: Drug-metabolizing enzymes (DMEs) inhibition based drug-drug interaction and herb-drug interaction severely challenge the R&D process of drugs or herbal ingredients. Objective: To evaluate the inhibition potential of wogonin (an important flavonoid isolated from the root of Scutellaria baicalensis ) towards one of the most important phase II DMEs, UDP-glucuronosyltransferase (UGT) 1A9. Methods: Both recombinant UGT1A9-catalyzed 4-methylumbelliferone (4-MU) glucuronidation reaction and human liver microsomes (HLMs)-catalyzed propofol glucuronidation reaction were used as two different probe reactions. Results: Wogonin noncompetitively inhibited recombinant UGT1A9-catalyzed 4-MU glucuronidation, and exerted competitive inhibition towards HLMs-catalyzed propofol glucuronidation. The inhibition kinetic parameters (Ki) were calculated to be 3.2 μM and 52.0μM, respectively. Conclusion: Necessary monitoring was needed when wogonin was co-administered with the clinical drugs mainly undergoing UGT1A9-mediated glucuronidation elimination. Additionally, probe reactions-dependent inhibition of wogonin towards the activity of UGT1A9 should be paid attention when translating these in vitro data into in vivo situation