Background: Herbs might affect the homeostasis of bile acids through
influence of multiple metabolic pathways of bile acids. Objective:To
investigate the inhibition of cryptotanshinone towards the
glucuronidation of LCA, trying to indicate the possible influence of
cryptotanshinone-containing herbs towards the homeostasis of bile
acids. Methods:The LCA-3-glucuronidation and LCA-24-glucuronidation
reaction was monitored by LC-MS. Results:Initial screening showed that
100 μM of cryptotanshinone inhibited LCA-24-glucuronidation and
LCA-3-glucuro-nidation reaction activity by 82.6% and 79.1%,
respectively. This kind of inhibition behaviour exerted
cryptotanshinone concentrations-dependent and LCA
concentrations-independent inhibition behaviour. Conclusion: All these
data indicated the possibility of cryptotanshinone’s influence
towards bile acids metabolism and homeostasis of bile acids